FastQCFastQC Report
Sat 18 Jun 2016
SRR3548617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255736
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC38161.4921637939124721No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT37831.4792598617324115No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT32391.2665404948853505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26191.0241029811993618No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT17410.6807801795601715No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT7700.3010917508680827No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240.24400162667751116No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT5990.23422592048049554No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT5420.21193731035129976No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG5300.20724497137673226No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.20137954765852284No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT4650.1818281352644915No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4040.15797541214377328No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT3470.13568680201457753No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3430.13412268902305505No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.13099446304001No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCC207.02299E-445.013
TCACGCC253.8822724E-545.043
CAGCGCG207.02299E-445.028
CCGGGTA207.02299E-445.05
GTACCGG253.8822724E-545.02
TACCCCC453.8198777E-1045.014
GTCGAAC253.8822724E-545.013
GGTCGAA253.8822724E-545.012
CTATCCG253.8822724E-545.01
GACAATG253.8822724E-545.014
GCTATGT207.02299E-445.015
GGCCGAT207.02299E-445.08
GATCACG253.8822724E-545.01
CAATTGG351.2069722E-745.02
CAATTAC207.02299E-445.022
CGACATG406.7793735E-945.01
TTCCGCA253.8822724E-545.041
CGCTGGA207.02299E-445.019
ATCACGC253.8822724E-545.042
TAGCACG207.02299E-445.01