##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255736
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00088372384021	31.0	31.0	33.0	28.0	34.0
2	31.18075280758282	31.0	31.0	34.0	28.0	34.0
3	31.379113617167704	31.0	31.0	34.0	28.0	34.0
4	35.33404761159946	37.0	35.0	37.0	33.0	37.0
5	34.95294366065004	35.0	35.0	37.0	32.0	37.0
6	34.95962633340633	35.0	35.0	37.0	32.0	37.0
7	32.55608518159352	35.0	35.0	37.0	25.0	37.0
8	33.99124878781243	36.0	35.0	37.0	28.0	37.0
9	34.32009963399756	38.0	34.0	39.0	28.0	39.0
10	34.84209497294084	37.0	34.0	39.0	27.0	39.0
11	35.77858807520255	37.0	35.0	39.0	30.0	39.0
12	35.82830340663809	37.0	35.0	39.0	31.0	39.0
13	35.96393546469797	37.0	35.0	39.0	31.0	39.0
14	36.90062017080114	39.0	36.0	40.0	31.0	41.0
15	36.98522695279507	39.0	36.0	40.0	31.0	41.0
16	36.89090702912378	38.0	35.0	40.0	31.0	41.0
17	36.813882284856255	38.0	35.0	40.0	31.0	41.0
18	36.58199080301561	38.0	35.0	40.0	31.0	41.0
19	36.49839287390121	38.0	35.0	40.0	30.0	41.0
20	36.60314934150843	38.0	35.0	40.0	31.0	41.0
21	36.71066255826321	38.0	35.0	40.0	31.0	41.0
22	36.71744298808146	38.0	35.0	40.0	31.0	41.0
23	36.65994228423061	38.0	35.0	40.0	31.0	41.0
24	36.31544639784778	38.0	35.0	40.0	30.0	41.0
25	36.43986376575844	38.0	35.0	40.0	30.0	41.0
26	36.33396940594989	38.0	35.0	40.0	30.0	41.0
27	36.230558075515376	38.0	35.0	40.0	30.0	41.0
28	36.09269715644258	38.0	35.0	40.0	30.0	41.0
29	35.73336174805268	38.0	34.0	40.0	29.0	41.0
30	35.72005896705978	38.0	34.0	40.0	29.0	41.0
31	35.644672631150875	38.0	34.0	40.0	29.0	41.0
32	35.53954859699065	38.0	34.0	40.0	28.0	41.0
33	35.53880564331968	38.0	34.0	40.0	28.0	41.0
34	35.30392670566522	38.0	34.0	40.0	27.0	41.0
35	35.26257546845184	38.0	34.0	40.0	27.0	41.0
36	35.14132542934902	38.0	34.0	40.0	26.0	41.0
37	34.93139409390935	38.0	34.0	40.0	25.0	41.0
38	34.887360402915505	38.0	34.0	40.0	25.0	41.0
39	34.80150624081084	38.0	33.0	40.0	24.0	41.0
40	34.6042246691901	37.0	33.0	40.0	24.0	41.0
41	34.55388369255795	37.0	33.0	40.0	24.0	41.0
42	34.51676729126912	37.0	33.0	40.0	24.0	41.0
43	34.52508837238402	37.0	33.0	40.0	24.0	41.0
44	34.339514186504836	37.0	33.0	40.0	23.0	41.0
45	34.11593987549661	37.0	33.0	40.0	23.0	41.0
46	34.01225873557106	37.0	33.0	40.0	23.0	41.0
47	33.85535865110896	37.0	33.0	40.0	22.0	41.0
48	33.781849250789875	37.0	33.0	40.0	22.0	41.0
49	33.62555526011199	36.0	32.0	40.0	21.0	41.0
50	33.42019504489004	36.0	32.0	40.0	20.0	41.0
51	31.393120249006788	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	7.0
12	4.0
13	9.0
14	17.0
15	25.0
16	64.0
17	127.0
18	260.0
19	467.0
20	819.0
21	1147.0
22	1581.0
23	2221.0
24	2595.0
25	2936.0
26	3373.0
27	3942.0
28	4765.0
29	6020.0
30	7409.0
31	9416.0
32	12113.0
33	15767.0
34	22234.0
35	25049.0
36	29122.0
37	38246.0
38	42125.0
39	23868.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.78599806049989	36.406685018925764	17.729220758907623	23.07809616166672
2	24.648074576907437	35.90069446616824	17.59001470266212	21.86121625426221
3	20.59545781587262	30.621813119779773	23.93874933525198	24.84397972909563
4	24.619138486564268	32.643429161322615	17.06447273751056	25.672959614602558
5	16.600713235524132	38.16944036037163	18.804157412331467	26.425688991772766
6	18.279006475427785	38.45919229205118	23.318969562361186	19.94283167015985
7	70.53875871992993	18.182813526449152	6.548550067256858	4.7298776863640635
8	77.05289830137329	7.542543873369412	4.966058748083961	10.438499077173336
9	70.01751806550504	15.11285075233835	9.19424719241718	5.6753839897394185
10	33.15958644852504	34.18642662745957	13.89049644946351	18.76349047455188
11	26.99854537491788	29.384599743485467	24.448650170488317	19.16820471110833
12	26.24386085650827	25.786357806487942	31.363984734257205	16.605796602746583
13	26.2090593424469	28.607626614946664	25.251431163387245	19.931882879219195
14	18.88744642913004	31.173553977539335	31.458613570244314	18.480386023086307
15	18.50032846372822	32.861622923640006	26.595786279600837	22.042262333030937
16	19.66090030343792	29.13629680608127	29.495260737635686	21.70754215284512
17	18.59534832796321	29.06121938248819	28.231457440485503	24.1119748490631
18	21.549175712453465	29.744736759783525	25.44420808959239	23.261879438170613
19	20.119967466449776	34.42651797165827	26.250899364970127	19.202615196921826
20	22.277661338255076	34.10626583664404	22.663997247161134	20.95207557793975
21	20.611489974035724	31.438280101354522	25.301482779115965	22.648747145493793
22	20.1700190821785	31.578659242343665	24.804094847811804	23.447226827666032
23	21.86317139550161	31.301420214596305	21.566380955360216	25.26902743454187
24	18.155832577345386	36.17871555041136	24.016172928332345	21.649278943910907
25	22.32341164325711	28.469984671692682	23.460912816341853	25.745690868708355
26	22.73633747301905	31.117636938092406	21.39550161103638	24.75052397785216
27	19.213172959614603	28.949776331842216	26.515234460537428	25.321816248005756
28	24.68835048643914	29.381471517502423	23.848812838239432	22.081365157819
29	20.310789251415525	33.21433040322833	22.431726467920043	24.043153877436104
30	24.46233615916414	28.906372196327464	23.59855475959583	23.032736884912566
31	27.545202865455	30.414959176650918	19.296070948165294	22.74376700972878
32	22.046563643757626	28.38747771138987	22.39809803860231	27.167860606250194
33	25.445381174336035	26.986814527481467	25.090327525260424	22.477476772922074
34	20.523508618262586	29.97700753902462	22.949838896361875	26.549644946350924
35	22.977601901961396	27.814621328244755	27.087699815434668	22.120076954359185
36	23.87110144836863	29.02055244470861	24.49400944724247	22.614336659680294
37	20.68343917164576	31.22438764976382	22.365642694028217	25.726530484562204
38	24.502612068695843	28.46372821972659	26.00455156880533	21.029108142772234
39	20.599759126599306	26.648575093064725	25.289751931679543	27.461913848656426
40	22.453624049801356	26.93324365752182	28.33116964369506	22.28196264898176
41	20.192307692307693	25.657709512935213	30.947148622016456	23.202834172740637
42	24.41384865642694	26.037788969875187	26.27670722933025	23.271655144367628
43	22.190462038977696	25.288578846935906	27.18936715988363	25.33159195420277
44	22.209622423123847	24.628914192761286	26.275534144586604	26.88592923952826
45	26.83978790627835	25.563080676948104	25.64949791972972	21.947633497043824
46	20.486751962961804	24.08303875871993	31.84964181812494	23.580567460193326
47	22.582663371601967	27.156129758813773	25.362092157537457	24.8991147120468
48	20.974364188068947	25.736306190759223	30.45914536866143	22.830184252510403
49	22.932633653455127	23.66072825100885	28.559530140457344	24.847107955078677
50	21.017377295335816	23.238417743297777	27.52017705759064	28.224027903775767
51	20.29866737573122	22.612772546688774	31.707307535896394	25.38125254168361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3074.0
1	2415.0
2	1756.0
3	1217.5
4	679.0
5	663.5
6	648.0
7	683.5
8	719.0
9	799.5
10	880.0
11	939.5
12	999.0
13	1050.0
14	1101.0
15	1069.5
16	1038.0
17	992.0
18	946.0
19	883.5
20	821.0
21	775.0
22	729.0
23	747.5
24	766.0
25	943.0
26	1369.5
27	1619.0
28	1914.0
29	2209.0
30	2636.0
31	3063.0
32	3917.5
33	4772.0
34	5115.5
35	5459.0
36	5816.5
37	6174.0
38	6642.5
39	7111.0
40	8297.0
41	9483.0
42	11072.0
43	12661.0
44	19211.0
45	25761.0
46	26270.5
47	26780.0
48	25959.5
49	25139.0
50	23819.5
51	22500.0
52	20619.5
53	18739.0
54	16816.5
55	14894.0
56	13531.0
57	12168.0
58	11509.0
59	10850.0
60	9824.5
61	8799.0
62	7507.0
63	6215.0
64	5343.0
65	4471.0
66	3755.5
67	3040.0
68	2642.0
69	2244.0
70	2030.5
71	1817.0
72	1669.0
73	1521.0
74	1360.0
75	899.0
76	599.0
77	572.5
78	546.0
79	424.5
80	303.0
81	217.0
82	131.0
83	107.5
84	84.0
85	77.0
86	70.0
87	46.0
88	22.0
89	15.5
90	9.0
91	7.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	255736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.00965057715769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42720846936511	29.025635811931057
2	8.799011062157279	6.512966496699722
3	3.320760298794468	3.687005349266431
4	1.7306412247614822	2.5620170801138675
5	1.0660665419928788	1.972737510557763
6	0.6962714084968357	1.5461256921199988
7	0.5261656470886558	1.3631244721118654
8	0.4162836645641172	1.232521037319736
9	0.36239923082612235	1.2071042012074953
>10	4.357243230107663	35.22226045609535
>50	0.25040413325303496	5.841570995088685
>100	0.03592295582532991	2.4842024587856226
>500	0.006339345145646455	1.3998811274126444
>1k	0.0052827876213720456	5.942847311289768
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC	3816	1.4921637939124721	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT	3783	1.4792598617324115	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	3239	1.2665404948853505	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2619	1.0241029811993618	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	1741	0.6807801795601715	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	770	0.3010917508680827	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	624	0.24400162667751116	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	599	0.23422592048049554	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	542	0.21193731035129976	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	530	0.20724497137673226	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	515	0.20137954765852284	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	465	0.1818281352644915	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	404	0.15797541214377328	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	347	0.13568680201457753	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	343	0.13412268902305505	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	335	0.13099446304001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.895063659398755	0.0
2	0.0	0.0	0.0	1.1085650827415772	0.0
3	0.0	0.0	0.0	1.5914849688741515	0.0
4	0.0	0.0	0.0	4.175790659117214	0.0
5	0.0	0.0	0.0	4.506209528576345	0.0
6	0.0	0.0	0.0	5.976084712359621	0.0
7	0.0	0.0	0.0	7.093643444802453	0.0
8	0.0	0.0	0.0	7.957033816122877	0.0
9	0.0	0.0	0.0	10.556198579785404	0.0
10	0.0	0.0	0.0	12.05696499515125	0.0
11	0.0	0.0	0.0	15.083523633747301	0.0
12	0.0	0.0	0.0	15.88747771138987	0.0
13	0.0	0.0	0.0	16.308224106109424	0.0
14	0.0	0.0	0.0	16.846670003441048	0.0
15	0.0	0.0	0.0	17.12938342665874	0.0
16	0.0	0.0	0.0	17.629508555698063	0.0
17	0.0	0.0	0.0	18.14605687114837	0.0
18	0.0	0.0	0.0	18.969953389432852	0.0
19	0.0	0.0	0.0	19.268698970813652	0.0
20	0.0	0.0	0.0	19.60967560296556	0.0
21	0.0	0.0	0.0	19.949479150373822	0.0
22	0.0	0.0	0.0	20.28419933055964	0.0
23	0.0	0.0	0.0	20.63338755591704	0.0
24	0.0	0.0	0.0	20.891857227766135	0.0
25	0.0	0.0	0.0	21.111615103075046	0.0
26	0.0	0.0	0.0	21.348969249538587	0.0
27	3.910282478806269E-4	0.0	0.0	21.597663215190664	0.0
28	3.910282478806269E-4	0.0	0.0	21.84361998310758	0.0
29	3.910282478806269E-4	0.0	0.0	22.109519191666404	0.0
30	3.910282478806269E-4	0.0	0.0	22.512278286983452	0.0
31	3.910282478806269E-4	0.0	0.0	22.769183845841024	0.0
32	3.910282478806269E-4	0.0	0.0	23.028044545938	0.0
33	3.910282478806269E-4	0.0	0.0	23.280648794068885	0.0
34	3.910282478806269E-4	0.0	0.0	23.536772296430694	0.0
35	3.910282478806269E-4	0.0	0.0	23.82026777614415	0.0
36	3.910282478806269E-4	0.0	0.0	24.065442487565303	0.0
37	3.910282478806269E-4	0.0	0.0	24.30944411424281	0.0
38	3.910282478806269E-4	0.0	0.0	24.562048362373698	0.0
39	3.910282478806269E-4	0.0	0.0	24.8768261019176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	20	7.02299E-4	45.0	13
TCACGCC	25	3.8822724E-5	45.0	43
CAGCGCG	20	7.02299E-4	45.0	28
CCGGGTA	20	7.02299E-4	45.0	5
GTACCGG	25	3.8822724E-5	45.0	2
TACCCCC	45	3.8198777E-10	45.0	14
GTCGAAC	25	3.8822724E-5	45.0	13
GGTCGAA	25	3.8822724E-5	45.0	12
CTATCCG	25	3.8822724E-5	45.0	1
GACAATG	25	3.8822724E-5	45.0	14
GCTATGT	20	7.02299E-4	45.0	15
GGCCGAT	20	7.02299E-4	45.0	8
GATCACG	25	3.8822724E-5	45.0	1
CAATTGG	35	1.2069722E-7	45.0	2
CAATTAC	20	7.02299E-4	45.0	22
CGACATG	40	6.7793735E-9	45.0	1
TTCCGCA	25	3.8822724E-5	45.0	41
CGCTGGA	20	7.02299E-4	45.0	19
ATCACGC	25	3.8822724E-5	45.0	42
TAGCACG	20	7.02299E-4	45.0	1
>>END_MODULE