SRR3548616_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548616_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262079
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2264	0.8638616600338066	No Hit
AATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	1986	0.7577867742169346	No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1791	0.6833817284101359	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	1749	0.6673560262363638	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGT	1668	0.636449314901232	No Hit
AAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	725	0.2766341446663029	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	550	0.20986038560891945	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	504	0.19230842608526438	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.1827693176484953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	449	0.1713223875243724	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	427	0.16292797210001564	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	407	0.1552966853506004	No Hit
AACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	393	0.1499547846260097	TruSeq Adapter, Index 16 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	304	0.11599555859111185	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	297	0.11332460822881651	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.10760114316675506	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	267	0.10187767810469361	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTGG	30	2.1590677E-6	45.000004	2
GTCACGG	30	2.1590677E-6	45.000004	2
CTCGCGG	30	2.1590677E-6	45.000004	2
ACCGTAG	30	2.1590677E-6	45.000004	26
AATGTTC	35	1.207136E-7	45.000004	19
CTTACGG	30	2.1590677E-6	45.000004	2
TATCCGG	30	2.1590677E-6	45.000004	2
TTAGACC	20	7.02331E-4	45.0	40
GACAACG	20	7.02331E-4	45.0	1
ATAGCGG	40	6.7811925E-9	45.0	2
TAAACGG	25	3.882539E-5	45.0	2
GAATCCT	25	3.882539E-5	45.0	13
ATTCGCG	20	7.02331E-4	45.0	1
TACGGTT	20	7.02331E-4	45.0	32
TAAAACG	25	3.882539E-5	45.0	21
CGCGACC	100	0.0	45.0	10
TCGATAT	20	7.02331E-4	45.0	25
ATACTAG	20	7.02331E-4	45.0	1
AGACGAA	20	7.02331E-4	45.0	14
CCGTAGT	25	3.882539E-5	45.0	27