##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548616_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262079
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979433682210328	31.0	31.0	33.0	28.0	34.0
2	31.147642504740936	31.0	31.0	34.0	28.0	34.0
3	31.353301866994304	31.0	31.0	34.0	28.0	34.0
4	35.35789971726083	37.0	35.0	37.0	33.0	37.0
5	34.97205804356702	35.0	35.0	37.0	32.0	37.0
6	34.97450005532683	35.0	35.0	37.0	32.0	37.0
7	33.8998279144838	35.0	35.0	37.0	30.0	37.0
8	34.6352168620912	37.0	35.0	37.0	32.0	37.0
9	35.929299943910046	39.0	35.0	39.0	32.0	39.0
10	35.60911404576483	37.0	35.0	39.0	30.0	39.0
11	36.07757966109455	37.0	35.0	39.0	31.0	39.0
12	36.038576154518296	37.0	35.0	39.0	31.0	39.0
13	36.02743447586415	37.0	35.0	39.0	31.0	39.0
14	37.034958924599074	39.0	36.0	40.0	32.0	41.0
15	37.02891876113691	39.0	36.0	40.0	32.0	41.0
16	36.95170540180633	38.0	36.0	40.0	31.0	41.0
17	36.86861595167869	38.0	35.0	40.0	31.0	41.0
18	36.653138939022206	38.0	35.0	40.0	31.0	41.0
19	36.598254724720405	38.0	35.0	40.0	31.0	41.0
20	36.652371994703884	38.0	35.0	40.0	31.0	41.0
21	36.738945127232626	38.0	35.0	40.0	31.0	41.0
22	36.70245994528367	38.0	35.0	40.0	31.0	41.0
23	36.63464833122837	38.0	35.0	40.0	31.0	41.0
24	36.32376878727407	38.0	35.0	40.0	30.0	41.0
25	36.402054342392944	38.0	35.0	40.0	30.0	41.0
26	36.34633831783546	38.0	35.0	40.0	30.0	41.0
27	36.26132578344698	38.0	35.0	40.0	30.0	41.0
28	36.09600540295102	38.0	35.0	40.0	30.0	41.0
29	35.8169750342454	38.0	34.0	40.0	29.0	41.0
30	35.81550219590276	38.0	34.0	40.0	29.0	41.0
31	35.627108619919944	38.0	34.0	40.0	28.0	41.0
32	35.57857745183704	38.0	34.0	40.0	28.0	41.0
33	35.58168720118743	38.0	34.0	40.0	28.0	41.0
34	35.45208887396549	38.0	34.0	40.0	27.0	41.0
35	35.482007333666566	38.0	34.0	40.0	27.0	41.0
36	35.22151717611865	38.0	34.0	40.0	26.0	41.0
37	35.24678818218934	38.0	34.0	40.0	27.0	41.0
38	35.136271887484305	38.0	34.0	40.0	26.0	41.0
39	34.96032875583317	38.0	34.0	40.0	25.0	41.0
40	34.80339134383144	38.0	33.0	40.0	25.0	41.0
41	34.73236314241126	38.0	33.0	40.0	25.0	41.0
42	34.694946943478875	37.0	33.0	40.0	25.0	41.0
43	34.63052362074031	37.0	33.0	40.0	24.0	41.0
44	34.5931265000248	37.0	33.0	40.0	24.0	41.0
45	34.33367038183143	37.0	33.0	40.0	24.0	41.0
46	34.18743966513914	37.0	33.0	40.0	23.0	41.0
47	34.022172703650426	37.0	33.0	40.0	23.0	41.0
48	33.87839926129144	36.0	33.0	40.0	23.0	41.0
49	33.654260738174365	36.0	33.0	40.0	22.0	41.0
50	33.52777216030281	36.0	32.0	39.0	21.0	40.0
51	31.43725365252462	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	4.0
12	11.0
13	8.0
14	18.0
15	40.0
16	77.0
17	129.0
18	258.0
19	442.0
20	784.0
21	1086.0
22	1600.0
23	1998.0
24	2353.0
25	2609.0
26	3077.0
27	3798.0
28	4558.0
29	5534.0
30	7137.0
31	9294.0
32	12046.0
33	15921.0
34	22454.0
35	25200.0
36	30336.0
37	39854.0
38	46580.0
39	24867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.796099649342374	34.10689143349906	18.512738525406462	25.584270391752106
2	24.81541825174852	34.07980036553864	18.63216816303481	22.472613219678035
3	21.94033096890632	31.132215858576995	21.464901804417753	25.462551368098936
4	22.207426005135854	32.95227774831253	17.740452306365636	27.099843940185973
5	17.576379641253208	35.807142121268775	17.42184608457755	29.194632152900464
6	19.19726494682901	38.92528588707985	21.025721251988905	20.851727914102234
7	76.40711388550781	11.889163191251493	6.697980379961767	5.005742543278934
8	79.63438505183551	6.876934054235555	5.69370304373872	7.79497785019021
9	73.38626902575179	11.003552363981854	9.882134776155281	5.728043834111088
10	32.18876750903353	33.635659476722665	14.216324085485674	19.95924892875812
11	26.22682473605287	30.162279312726316	24.203007490107943	19.407888461112872
12	25.59113854982658	24.909283078766325	30.797202370277667	18.70237600112943
13	25.53314077053102	27.56268148153801	26.321834256083086	20.58234349184788
14	19.340733137718015	31.182200786785664	30.139385452478066	19.33768062301825
15	18.383388215003873	31.2039499540215	28.566958817761055	21.845703013213573
16	19.88293606126397	28.337638650941127	27.884721782363332	23.89470350543157
17	18.525711712880465	29.27933943581897	26.600376222436743	25.594572628863816
18	21.79953372837961	28.186157608965235	25.374410006143183	24.639898656511967
19	20.8639379729013	33.062168277504114	25.219494885130057	20.85439886446453
20	23.182322887373655	31.062008020482374	24.188126480946583	21.56754261119739
21	21.185215145051682	30.845279476798982	25.103880890876418	22.86562448727292
22	20.290446773682742	29.116793028056424	25.947138076686798	24.64562212157403
23	21.50840013888942	31.0551398624079	22.148283532827886	25.288176465874795
24	19.70207456530283	31.152820332800413	25.489260871721886	23.65584423017487
25	21.926213088419903	28.70966387997512	23.73978838441844	25.624334647186537
26	23.501692237836682	29.514764632038432	22.39439253049653	24.589150599628358
27	19.161016334769286	27.174248985992772	25.54764021535491	28.11709446388303
28	20.584251313535233	29.71279652318576	27.29596800964595	22.406984153633065
29	19.980616531656487	33.18617668718211	23.35173745321067	23.481469327950734
30	21.780837075843543	27.38868814365134	25.352660838907354	25.47781394159776
31	23.73063084031914	32.68594584075794	20.485807714467775	23.097615604455147
32	21.147821839979546	27.45851441740849	22.354328275062098	29.039335467549858
33	21.1001262977957	26.623651647022463	24.459037160550825	27.81718489463101
34	19.44299238016018	30.648773843001536	23.815719687575122	26.09251408926316
35	19.430019192686174	27.286047336871704	25.129445701486958	28.15448776895516
36	21.00282739174066	31.575212054380547	23.51542855398563	23.90653199989316
37	19.070967151126187	30.820096230525913	20.16949087870451	29.93944573964339
38	20.870806130975776	29.993246311226766	22.48520484281457	26.65074271498289
39	20.436967479271516	25.774289431812548	23.62684534052709	30.161897748388846
40	22.79923229255301	26.6858466340302	22.8045741932776	27.7103468801392
41	20.066468507587405	24.99017471831013	27.366175847740564	27.577180926361898
42	23.0903658820432	27.974770966006435	22.635541191778053	26.299321960172318
43	19.916895287298868	27.094120475123912	24.978727788186006	28.010256449391214
44	22.13950755306606	23.455904517340194	24.79710316354992	29.60748476604383
45	24.921874701902862	26.02192468683107	24.362882947508194	24.693317663757874
46	21.0531938842868	23.623792825827326	29.130529344205375	26.1924839456805
47	22.221925449959745	26.330228671507445	24.392263401493445	27.055582477039366
48	21.14896653299196	25.691108406243917	27.89845809851228	25.261466962251838
49	21.422166598621025	23.232307815582324	28.04803131880082	27.29749426699583
50	20.622026182944836	23.57075538291889	26.749567878387815	29.05765055574846
51	20.625460261982074	21.984592432052928	29.775754638868435	27.61419266709656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2663.0
1	2094.0
2	1525.0
3	1048.5
4	572.0
5	532.5
6	493.0
7	553.0
8	613.0
9	669.5
10	726.0
11	747.5
12	769.0
13	804.5
14	840.0
15	834.5
16	829.0
17	804.0
18	779.0
19	742.5
20	706.0
21	749.5
22	793.0
23	778.5
24	764.0
25	787.0
26	1233.0
27	1656.0
28	2030.0
29	2404.0
30	2574.0
31	2744.0
32	3344.5
33	3945.0
34	4214.5
35	4484.0
36	5395.5
37	6307.0
38	6569.5
39	6832.0
40	8264.5
41	9697.0
42	11190.5
43	12684.0
44	17846.5
45	23009.0
46	24052.0
47	25095.0
48	25979.0
49	26863.0
50	25946.5
51	25030.0
52	22437.5
53	19845.0
54	17681.0
55	15517.0
56	13632.0
57	11747.0
58	11124.5
59	10502.0
60	9971.0
61	9440.0
62	8306.0
63	7172.0
64	6792.0
65	6412.0
66	5676.0
67	4940.0
68	4205.0
69	3470.0
70	3259.0
71	3048.0
72	2690.5
73	2333.0
74	1828.5
75	1156.0
76	988.0
77	796.0
78	604.0
79	546.5
80	489.0
81	383.5
82	278.0
83	205.5
84	133.0
85	111.5
86	90.0
87	57.5
88	25.0
89	27.0
90	29.0
91	27.5
92	26.0
93	25.0
94	24.0
95	15.0
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.17434989082136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.515	30.354334315686604
2	8.396	6.410236833666723
3	2.827	3.237566614240559
4	1.428	2.180518865763716
5	0.899	1.71593702759242
6	0.63	1.4429904258730473
7	0.541	1.4456626303654048
8	0.44600000000000006	1.3620608041045061
9	0.42700000000000005	1.4670402663042648
>10	4.644	38.07166088961505
>50	0.192	4.641619203224969
>100	0.047	3.3807204263311394
>500	0.003	0.6791216845577119
>1k	0.005	3.610530012673884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2264	0.8638616600338066	No Hit
AATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	1986	0.7577867742169346	No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1791	0.6833817284101359	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	1749	0.6673560262363638	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGT	1668	0.636449314901232	No Hit
AAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	725	0.2766341446663029	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	550	0.20986038560891945	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	504	0.19230842608526438	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.1827693176484953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	449	0.1713223875243724	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	427	0.16292797210001564	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	407	0.1552966853506004	No Hit
AACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	393	0.1499547846260097	TruSeq Adapter, Index 16 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	304	0.11599555859111185	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	297	0.11332460822881651	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.10760114316675506	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	267	0.10187767810469361	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8817951838949325	0.0
2	0.0	0.0	0.0	1.1569030712113524	0.0
3	0.0	0.0	0.0	1.6086752467767353	0.0
4	0.0	0.0	0.0	3.9198104388371444	0.0
5	0.0	0.0	0.0	4.360135684278404	0.0
6	0.0	0.0	0.0	6.114568507968971	0.0
7	0.0	0.0	0.0	7.421426363806333	0.0
8	0.0	0.0	0.0	8.303221547701266	0.0
9	0.0	0.0	0.0	10.023695145356934	0.0
10	0.0	0.0	0.0	11.715169853364825	0.0
11	0.0	0.0	0.0	13.833615055002499	0.0
12	0.0	0.0	0.0	14.781420869279874	0.0
13	0.0	0.0	0.0	15.249218747019029	0.0
14	0.0	0.0	0.0	15.741055178018842	0.0
15	0.0	0.0	0.0	16.082173695717703	0.0
16	0.0	0.0	0.0	16.663296181685674	0.0
17	0.0	0.0	0.0	17.356980147207523	0.0
18	0.0	0.0	0.0	18.416202748026357	0.0
19	0.0	0.0	0.0	18.794333006459883	0.0
20	0.0	0.0	0.0	19.285024744447284	0.0
21	0.0	0.0	0.0	19.70360082265271	0.0
22	0.0	0.0	0.0	20.12828193025767	0.0
23	0.0	0.0	0.0	20.60332953040877	0.0
24	0.0	0.0	0.0	20.951697770519576	0.0
25	0.0	0.0	0.0	21.249699518084242	0.0
26	0.0	0.0	0.0	21.494282258403	0.0
27	0.0	0.0	0.0	21.83501921176439	0.0
28	0.0	0.0	0.0	22.172322086088545	0.0
29	0.0	0.0	0.0	22.48024450642745	0.0
30	0.0	0.0	0.0	22.96521277935279	0.0
31	0.0	0.0	0.0	23.251004468118392	0.0
32	0.0	0.0	0.0	23.581057620030602	0.0
33	0.0	0.0	0.0	23.93095211749129	0.0
34	0.0	0.0	0.0	24.22551978601872	0.0
35	0.0	0.0	0.0	24.603268480114775	0.0
36	0.0	0.0	0.0	24.902796485029324	0.0
37	0.0	0.0	0.0	25.19812728223169	0.0
38	0.0	0.0	0.0	25.492313386421653	0.0
39	0.0	0.0	0.0	25.81587994459686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTGG	30	2.1590677E-6	45.000004	2
GTCACGG	30	2.1590677E-6	45.000004	2
CTCGCGG	30	2.1590677E-6	45.000004	2
ACCGTAG	30	2.1590677E-6	45.000004	26
AATGTTC	35	1.207136E-7	45.000004	19
CTTACGG	30	2.1590677E-6	45.000004	2
TATCCGG	30	2.1590677E-6	45.000004	2
TTAGACC	20	7.02331E-4	45.0	40
GACAACG	20	7.02331E-4	45.0	1
ATAGCGG	40	6.7811925E-9	45.0	2
TAAACGG	25	3.882539E-5	45.0	2
GAATCCT	25	3.882539E-5	45.0	13
ATTCGCG	20	7.02331E-4	45.0	1
TACGGTT	20	7.02331E-4	45.0	32
TAAAACG	25	3.882539E-5	45.0	21
CGCGACC	100	0.0	45.0	10
TCGATAT	20	7.02331E-4	45.0	25
ATACTAG	20	7.02331E-4	45.0	1
AGACGAA	20	7.02331E-4	45.0	14
CCGTAGT	25	3.882539E-5	45.0	27
>>END_MODULE