SRR3548615_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301582
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2624	0.8700784529580677	No Hit
AATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	2129	0.7059439886995909	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	1988	0.6591905352441458	No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1920	0.6366427704571228	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGT	1910	0.6333269226943252	No Hit
AAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	730	0.24205688668421854	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	564	0.1870138138217798	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	530	0.17573993142826827	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	490	0.1624765403770782	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	488	0.1618133708245187	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	459	0.1521974123124059	No Hit
AACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	456	0.15120265798356666	TruSeq Adapter, Index 16 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	452	0.14987631887844766	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	320	0.10610712840952047	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	313	0.10378603497556221	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	25	3.883939E-5	45.000004	2
ATTCGCG	30	2.1601572E-6	45.000004	1
CACTGCG	30	2.1601572E-6	45.000004	1
ACTAGTG	25	3.883939E-5	45.000004	23
ACCTACG	25	3.883939E-5	45.000004	28
ATATACG	25	3.883939E-5	45.000004	1
ACTACCG	25	3.883939E-5	45.000004	1
ACATACG	25	3.883939E-5	45.000004	17
GATTATG	25	3.883939E-5	45.000004	37
CGTATTT	20	7.025003E-4	45.0	42
ATTAGTA	20	7.025003E-4	45.0	33
CTCGTAA	40	6.7866495E-9	45.0	33
ACGTTGA	20	7.025003E-4	45.0	30
TTTGTCG	20	7.025003E-4	45.0	16
CGTTGAC	20	7.025003E-4	45.0	31
TAACGGA	20	7.025003E-4	45.0	10
GCGTTGA	20	7.025003E-4	45.0	42
CCAGACG	20	7.025003E-4	45.0	1
CACTAAG	20	7.025003E-4	45.0	20
GTCACTC	20	7.025003E-4	45.0	25