##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301582
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.992754872638287	31.0	31.0	33.0	28.0	34.0
2	31.17240087273113	31.0	31.0	34.0	28.0	34.0
3	31.36735614194481	31.0	31.0	34.0	28.0	34.0
4	35.36369544601468	37.0	35.0	37.0	33.0	37.0
5	35.01124735561141	35.0	35.0	37.0	32.0	37.0
6	35.00079580346307	35.0	35.0	37.0	32.0	37.0
7	32.593105689331594	35.0	35.0	37.0	26.0	37.0
8	34.03628200622053	36.0	35.0	37.0	28.0	37.0
9	34.22371361686042	38.0	34.0	39.0	27.0	39.0
10	34.78156852862571	37.0	34.0	39.0	27.0	39.0
11	35.78144252641073	37.0	35.0	39.0	30.0	39.0
12	35.95050434044472	37.0	35.0	39.0	31.0	39.0
13	36.051272953956136	37.0	35.0	39.0	32.0	39.0
14	37.111876703516785	39.0	36.0	40.0	32.0	41.0
15	37.11316325244875	39.0	36.0	40.0	32.0	41.0
16	37.057894701938444	39.0	36.0	40.0	32.0	41.0
17	36.96301834990152	38.0	36.0	40.0	32.0	41.0
18	36.782755602124794	38.0	35.0	40.0	31.0	41.0
19	36.702687826196524	39.0	35.0	40.0	31.0	41.0
20	36.744444297073436	38.0	35.0	40.0	31.0	41.0
21	36.82293041361885	39.0	35.0	40.0	31.0	41.0
22	36.7942350670796	39.0	35.0	40.0	31.0	41.0
23	36.74395023575678	38.0	35.0	40.0	31.0	41.0
24	36.43725421278458	38.0	35.0	40.0	30.0	41.0
25	36.49419395056734	38.0	35.0	40.0	30.0	41.0
26	36.446047841051524	38.0	35.0	40.0	30.0	41.0
27	36.37769495526921	38.0	35.0	40.0	30.0	41.0
28	36.22620381853029	38.0	35.0	40.0	30.0	41.0
29	35.85920910399162	38.0	34.0	40.0	29.0	41.0
30	35.892012785908975	38.0	35.0	40.0	29.0	41.0
31	35.751205310661774	38.0	34.0	40.0	29.0	41.0
32	35.77484730521052	38.0	34.0	40.0	29.0	41.0
33	35.737129536908704	38.0	34.0	40.0	29.0	41.0
34	35.596086636470346	38.0	34.0	40.0	28.0	41.0
35	35.56072975177564	38.0	34.0	40.0	28.0	41.0
36	35.31587760542738	38.0	34.0	40.0	27.0	41.0
37	35.34528254338787	38.0	34.0	40.0	27.0	41.0
38	35.211362083944	38.0	34.0	40.0	27.0	41.0
39	35.11233429050805	38.0	34.0	40.0	26.0	41.0
40	34.94668116797422	38.0	34.0	40.0	25.0	41.0
41	34.82237666704246	38.0	33.0	40.0	25.0	41.0
42	34.79711985463324	38.0	33.0	40.0	25.0	41.0
43	34.721309627232394	38.0	33.0	40.0	24.0	41.0
44	34.629802176522475	37.0	33.0	40.0	24.0	41.0
45	34.37917050752366	37.0	33.0	40.0	24.0	41.0
46	34.2642962776293	37.0	33.0	40.0	23.0	41.0
47	34.14641125796632	37.0	33.0	40.0	23.0	41.0
48	34.02843339456599	37.0	33.0	40.0	23.0	41.0
49	33.816819306192016	36.0	33.0	40.0	22.0	41.0
50	33.61824644706912	36.0	33.0	40.0	22.0	41.0
51	31.568495467236108	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	5.0
12	5.0
13	11.0
14	14.0
15	34.0
16	77.0
17	136.0
18	264.0
19	484.0
20	833.0
21	1201.0
22	1702.0
23	2317.0
24	2700.0
25	3084.0
26	3522.0
27	4313.0
28	5148.0
29	6438.0
30	8353.0
31	10642.0
32	13732.0
33	18200.0
34	25875.0
35	30120.0
36	36786.0
37	47953.0
38	50800.0
39	26824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.813636092339728	33.95726535403307	18.586653049585188	25.642445504042016
2	25.009781750900252	33.50067311709585	18.858884150910864	22.630660981093037
3	21.924385407617166	31.061535502781997	21.54140499101405	25.472674098586783
4	21.947927926733026	32.93034730189468	17.819365877273842	27.302358894098454
5	17.69170573840614	35.59330464019736	17.366089488099423	29.34890013329708
6	19.073419501163862	39.00232772512948	21.05231744600142	20.871935327705234
7	73.7086430887785	15.16569291270699	6.344211524560485	4.781452473954015
8	79.88142528400236	6.823683111061005	5.739400892626217	7.555490712310417
9	70.53570836455756	14.640462627079865	9.354669708404348	5.46915929995822
10	32.36764793654794	33.63264385805519	14.308214681247556	19.69149352414932
11	26.37292676618631	30.185488523850896	24.115829194050043	19.325755515912753
12	25.481626887546337	24.990549833876027	30.947470339741763	18.580352938835873
13	25.42558906035506	27.634938424707045	26.33247342348018	20.606999091457713
14	19.261759654090763	31.347693164711423	30.156309063538274	19.23423811765954
15	18.184109131181568	31.198811600161815	28.796811480791295	21.820267787865326
16	19.802906008979313	28.478158510786457	28.001339602496174	23.717595877738063
17	18.67385984574676	29.028257654634558	26.49030777698934	25.807574722629333
18	21.95721230046886	28.145247395401583	25.376182928689378	24.52135737544018
19	20.804292033344165	33.099455537797354	25.325782042694854	20.77047038616363
20	23.314388789781884	31.007487184248394	24.173524945122722	21.504599080847
21	21.2917216544754	30.624175182869006	25.13313128767632	22.950971874979274
22	20.21241320768481	29.14464391110875	25.907713325065817	24.735229556140617
23	21.66608086689524	31.034677135903337	22.191974322074927	25.1072676751265
24	19.67823013309813	31.369909344722164	25.431557586328097	23.520302935851607
25	21.764893130226607	28.58592356307737	23.92019417604499	25.72898913065103
26	23.443375267754707	29.537571871000257	22.35909304931992	24.659959811925113
27	19.039266269207047	27.088486713398012	25.688535787944904	28.183711229450033
28	20.459112281236944	29.776976079474238	27.357401966960893	22.406509672327925
29	19.991246161906215	33.104097724665266	23.455643904477057	23.449012208951462
30	21.94759634195675	27.420071489677767	25.28035492834453	25.351977240020958
31	23.747106922826962	32.780139398239946	20.48961808065468	22.983135598278412
32	20.987989999403148	27.557347587057585	22.517922157157923	28.93674025638135
33	21.1819670935268	26.87693562613153	24.34462268968307	27.596474590658595
34	19.395056734155222	30.736914006804117	23.812097538977792	26.05593172006287
35	19.577096776332805	27.33253310873991	25.237912076980724	27.85245803794656
36	20.98334781253523	31.54067550450624	23.717595877738063	23.758380805220472
37	19.15366301702356	30.901380055838878	20.377210841495845	29.567746085641716
38	20.801307770357646	29.95271601090251	22.575617908230598	26.670358310509247
39	20.55692979023947	25.96441432180966	23.437406741781672	30.041249146169203
40	22.673103832456846	26.81061867087558	22.969872207227223	27.54640528944035
41	20.282377595479836	24.887758553229304	27.379618146971634	27.450245704319226
42	22.928755694968533	28.071303990291195	22.661829950063332	26.338110364676936
43	19.974335338315946	27.050022879349562	24.995192020743946	27.98044976159055
44	22.10675703457103	23.498086755840866	24.903343037714453	29.491813171873655
45	24.862558110232044	25.909702833723497	24.562142302922588	24.66559675312187
46	21.084812754076836	23.64928941382443	29.220245240100535	26.0456525919982
47	22.101451678150553	26.39680087007845	24.527325901413214	26.97442155035778
48	21.164061515607695	25.64974036912017	27.850136944512606	25.33606117075953
49	21.3441120491276	23.216239696003075	28.26992327128277	27.16972498358655
50	20.69022687030393	23.56904589796473	26.791718338627636	28.949008893103702
51	20.605672752352593	22.123336273385018	30.064791665285064	27.206199308977325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3112.0
1	2370.0
2	1628.0
3	1158.0
4	688.0
5	667.0
6	646.0
7	675.0
8	704.0
9	731.0
10	758.0
11	832.5
12	907.0
13	930.0
14	953.0
15	972.0
16	991.0
17	943.0
18	895.0
19	842.5
20	790.0
21	832.0
22	874.0
23	850.0
24	826.0
25	960.0
26	1555.0
27	2016.0
28	2451.0
29	2886.0
30	3120.0
31	3354.0
32	3982.5
33	4611.0
34	5001.0
35	5391.0
36	6474.0
37	7557.0
38	7963.0
39	8369.0
40	9799.5
41	11230.0
42	13216.5
43	15203.0
44	21028.0
45	26853.0
46	27775.0
47	28697.0
48	29978.0
49	31259.0
50	29832.0
51	28405.0
52	25328.5
53	22252.0
54	19826.5
55	17401.0
56	15252.0
57	13103.0
58	12567.5
59	12032.0
60	11301.0
61	10570.0
62	9354.0
63	8138.0
64	7761.5
65	7385.0
66	6402.0
67	5419.0
68	4790.5
69	4162.0
70	3790.0
71	3418.0
72	2991.5
73	2565.0
74	2047.0
75	1315.0
76	1101.0
77	883.5
78	666.0
79	585.0
80	504.0
81	407.5
82	311.0
83	240.0
84	169.0
85	116.5
86	64.0
87	47.5
88	31.0
89	29.0
90	27.0
91	20.0
92	13.0
93	15.5
94	18.0
95	11.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	301582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.27326096343289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92378642403482	26.428062709034904
2	10.27066964418859	7.245600212573451
3	3.9816683591411857	4.21339281305492
4	2.023229478508105	2.8546360553730645
5	1.2130279942463198	2.1393726498500003
6	0.8104775547320877	1.7152911775841935
7	0.671050605868524	1.6569100198329139
8	0.5127414156848256	1.4468849409768698
9	0.40023621949798505	1.270587295563321
>10	4.861573503778574	37.04754074742825
>50	0.2688152435018657	5.9641718578087515
>100	0.05475866071334302	3.6646136334119013
>500	0.002986836038909619	0.6405611180971067
>1k	0.004978060064849365	3.7123747694103706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2624	0.8700784529580677	No Hit
AATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	2129	0.7059439886995909	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	1988	0.6591905352441458	No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1920	0.6366427704571228	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGT	1910	0.6333269226943252	No Hit
AAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	730	0.24205688668421854	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	564	0.1870138138217798	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	530	0.17573993142826827	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	490	0.1624765403770782	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	488	0.1618133708245187	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	459	0.1521974123124059	No Hit
AACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	456	0.15120265798356666	TruSeq Adapter, Index 16 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	452	0.14987631887844766	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	320	0.10610712840952047	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	313	0.10378603497556221	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.315847762797514E-4	0.0	0.0	0.8223302451737836	0.0
2	3.315847762797514E-4	0.0	0.0	1.0816295402245493	0.0
3	3.315847762797514E-4	0.0	0.0	1.517000351479863	0.0
4	3.315847762797514E-4	0.0	0.0	3.620574172198606	0.0
5	3.315847762797514E-4	0.0	0.0	4.022454921049665	0.0
6	3.315847762797514E-4	0.0	0.0	5.573940089262622	0.0
7	3.315847762797514E-4	0.0	0.0	6.742444840872466	0.0
8	3.315847762797514E-4	0.0	0.0	7.568090933809047	0.0
9	3.315847762797514E-4	0.0	0.0	9.202140711315662	0.0
10	3.315847762797514E-4	0.0	0.0	10.861722516595819	0.0
11	3.315847762797514E-4	0.0	0.0	12.974912295826673	0.0
12	3.315847762797514E-4	0.0	0.0	13.906333932396496	0.0
13	3.315847762797514E-4	0.0	0.0	14.378510653818863	0.0
14	3.315847762797514E-4	0.0	0.0	14.863950766292419	0.0
15	3.315847762797514E-4	0.0	0.0	15.187909092717735	0.0
16	3.315847762797514E-4	0.0	0.0	15.758234907918908	0.0
17	3.315847762797514E-4	0.0	0.0	16.440967962278915	0.0
18	3.315847762797514E-4	0.0	0.0	17.50203924637412	0.0
19	3.315847762797514E-4	0.0	0.0	17.88999343462143	0.0
20	3.315847762797514E-4	0.0	0.0	18.359185893057276	0.0
21	3.315847762797514E-4	0.0	0.0	18.79986206073307	0.0
22	3.315847762797514E-4	0.0	0.0	19.25015418692097	0.0
23	3.315847762797514E-4	0.0	0.0	19.70442533042423	0.0
24	3.315847762797514E-4	0.0	0.0	20.028383656849545	0.0
25	3.315847762797514E-4	0.0	0.0	20.306914868924537	0.0
26	3.315847762797514E-4	0.0	0.0	20.561571977107388	0.0
27	3.315847762797514E-4	0.0	0.0	20.885530303532704	0.0
28	3.315847762797514E-4	0.0	0.0	21.204183273537545	0.0
29	3.315847762797514E-4	0.0	0.0	21.506920174280957	0.0
30	3.315847762797514E-4	0.0	0.0	21.993686625859635	0.0
31	3.315847762797514E-4	0.0	0.0	22.29178133973513	0.0
32	3.315847762797514E-4	0.0	0.0	22.604797368543217	0.0
33	3.315847762797514E-4	0.0	0.0	22.964235266030467	0.0
34	3.315847762797514E-4	0.0	0.0	23.276588125285993	0.0
35	3.315847762797514E-4	0.0	0.0	23.645310396509075	0.0
36	3.315847762797514E-4	0.0	0.0	23.93080488888594	0.0
37	3.315847762797514E-4	0.0	0.0	24.214641457381408	0.0
38	3.315847762797514E-4	0.0	0.0	24.49284108468012	0.0
39	3.315847762797514E-4	0.0	0.0	24.830725971709185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	25	3.883939E-5	45.000004	2
ATTCGCG	30	2.1601572E-6	45.000004	1
CACTGCG	30	2.1601572E-6	45.000004	1
ACTAGTG	25	3.883939E-5	45.000004	23
ACCTACG	25	3.883939E-5	45.000004	28
ATATACG	25	3.883939E-5	45.000004	1
ACTACCG	25	3.883939E-5	45.000004	1
ACATACG	25	3.883939E-5	45.000004	17
GATTATG	25	3.883939E-5	45.000004	37
CGTATTT	20	7.025003E-4	45.0	42
ATTAGTA	20	7.025003E-4	45.0	33
CTCGTAA	40	6.7866495E-9	45.0	33
ACGTTGA	20	7.025003E-4	45.0	30
TTTGTCG	20	7.025003E-4	45.0	16
CGTTGAC	20	7.025003E-4	45.0	31
TAACGGA	20	7.025003E-4	45.0	10
GCGTTGA	20	7.025003E-4	45.0	42
CCAGACG	20	7.025003E-4	45.0	1
CACTAAG	20	7.025003E-4	45.0	20
GTCACTC	20	7.025003E-4	45.0	25
>>END_MODULE