FastQCFastQC Report
Sat 18 Jun 2016
SRR3548614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214285
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT24971.1652705509018364No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC22741.0612035373451245No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT22491.0495368317894394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21170.9879366264554216No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT16310.7611358704529015No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT7160.3341344471148237No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT6680.31173437244790814No Hit
AACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG5470.25526751755839183No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT5280.2464008213360711No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.22960076533588444No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT4140.19320064400214668No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.1885339617798726No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3530.16473388244627482No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTAT2970.13860046200154No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC2730.12740042466808224No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.11013370044566816No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT2340.10920036400121334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT253.880151E-545.0000046
CATATGG253.880151E-545.0000042
CGTGCAA253.880151E-545.00000432
GGACTAA253.880151E-545.0000048
TCGTGCA253.880151E-545.00000431
TGGATCC253.880151E-545.00000430
TTTGATC253.880151E-545.00000410
CGATAGG253.880151E-545.0000042
TCTATTC253.880151E-545.00000432
AACACCG253.880151E-545.0000041
CGGGTAC207.02043E-445.06
TAATAGG351.2057535E-745.02
GGTACGG207.02043E-445.08
CATGCTC207.02043E-445.032
GAATGGT207.02043E-445.010
TGGACGG207.02043E-445.02
TCCTATC207.02043E-445.026
ATTCCGG207.02043E-445.02
CCCGTGC207.02043E-445.028
ACTAGCA207.02043E-445.035