FastQCFastQC Report
Sat 18 Jun 2016
SRR3548613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248917
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT26471.0634066777279172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26441.0622014567104698No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT26151.0505509868751433No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC25361.0188135000823568No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT17610.7074647372417312No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT7810.3137592048755208No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT7110.28563738113507714No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.23903550179377062No Hit
AACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG5460.2193502251754601No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT5360.21533282178396815No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT4360.1751587878690487No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.1707396441384076No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3940.15828569362478256No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTAT3500.140609118702218No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC3350.1345830136149801No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.12895864886689137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCA253.8819722E-545.00000431
TACGCAG253.8819722E-545.00000425
CTAAACG351.2068085E-745.0000041
ACTACCG253.8819722E-545.0000041
CGGGATG351.2068085E-745.0000046
ACGTAGG502.1827873E-1145.0000042
AATACGG253.8819722E-545.0000042
CCCGCTG253.8819722E-545.00000435
GGCTACC253.8819722E-545.0000048
AATCCGG207.022628E-445.02
CGAAAGA207.022628E-445.029
GGCAACG207.022628E-445.028
TAACGGG800.045.03
TAGCCCG207.022628E-445.01
ATCGTTC207.022628E-445.026
CTAACGG406.7775545E-945.02
AATTGGC207.022628E-445.041
CGCTAAC406.7775545E-945.038
GTCACGG207.022628E-445.02
ACGGAAC207.022628E-445.05