##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248917
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.918780155634206	31.0	31.0	33.0	28.0	34.0
2	31.088816754179103	31.0	31.0	34.0	28.0	34.0
3	31.266839950666288	31.0	31.0	34.0	28.0	34.0
4	35.22719621399904	37.0	35.0	37.0	33.0	37.0
5	34.90817421068067	35.0	35.0	37.0	32.0	37.0
6	34.90356223158724	35.0	35.0	37.0	32.0	37.0
7	32.44938674337229	35.0	35.0	37.0	25.0	37.0
8	33.90306809097008	35.0	35.0	37.0	27.0	37.0
9	34.17704696746305	37.0	34.0	39.0	27.0	39.0
10	34.74772715403126	37.0	34.0	39.0	27.0	39.0
11	35.679969628430364	37.0	35.0	39.0	30.0	39.0
12	35.8161756730155	37.0	35.0	39.0	31.0	39.0
13	35.93418288023719	37.0	35.0	39.0	31.0	39.0
14	37.00419818654411	39.0	36.0	40.0	31.0	41.0
15	37.03950312754854	39.0	36.0	40.0	32.0	41.0
16	36.96821832176991	38.0	36.0	40.0	31.0	41.0
17	36.90570752499829	38.0	36.0	40.0	31.0	41.0
18	36.668664655286705	38.0	35.0	40.0	31.0	41.0
19	36.56894466830309	38.0	35.0	40.0	30.0	41.0
20	36.58389744372622	38.0	35.0	40.0	31.0	41.0
21	36.73240076009272	38.0	35.0	40.0	31.0	41.0
22	36.67120767163352	38.0	35.0	40.0	31.0	41.0
23	36.617233857068825	38.0	35.0	40.0	31.0	41.0
24	36.29582953353929	38.0	35.0	40.0	30.0	41.0
25	36.36427805252353	38.0	35.0	40.0	30.0	41.0
26	36.249183462760676	38.0	35.0	40.0	30.0	41.0
27	36.12115283407722	38.0	35.0	40.0	30.0	41.0
28	36.02553059855293	38.0	35.0	40.0	30.0	41.0
29	35.59016459301695	38.0	34.0	40.0	27.0	41.0
30	35.669327526846295	38.0	34.0	40.0	28.0	41.0
31	35.534941365997504	38.0	34.0	40.0	27.0	41.0
32	35.41904731295974	38.0	34.0	40.0	27.0	41.0
33	35.33296641049024	38.0	34.0	40.0	27.0	41.0
34	35.186511969853406	38.0	34.0	40.0	26.0	41.0
35	35.09810499082023	38.0	34.0	40.0	26.0	41.0
36	34.772293575770234	38.0	33.0	40.0	24.0	41.0
37	34.8288304936987	38.0	33.0	40.0	25.0	41.0
38	34.70049454235749	38.0	33.0	40.0	24.0	41.0
39	34.54668021870744	37.0	33.0	40.0	24.0	41.0
40	34.38810527203847	37.0	33.0	40.0	23.0	41.0
41	34.259648798595514	37.0	33.0	40.0	23.0	41.0
42	34.20866393215409	37.0	33.0	40.0	23.0	41.0
43	34.12720304358481	37.0	33.0	40.0	23.0	41.0
44	34.04893599071176	37.0	33.0	40.0	23.0	41.0
45	33.82915188597002	37.0	33.0	40.0	22.0	41.0
46	33.66702153729958	37.0	32.0	40.0	21.0	41.0
47	33.54522591867972	36.0	32.0	40.0	20.0	41.0
48	33.41380460153385	36.0	32.0	40.0	20.0	41.0
49	33.24265518224951	36.0	32.0	39.0	20.0	41.0
50	32.97055243314036	36.0	31.0	39.0	18.0	40.0
51	30.93274063241964	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	5.0
12	2.0
13	8.0
14	18.0
15	31.0
16	58.0
17	117.0
18	273.0
19	518.0
20	846.0
21	1219.0
22	1845.0
23	2351.0
24	2708.0
25	3099.0
26	3559.0
27	4129.0
28	4925.0
29	5921.0
30	7455.0
31	9306.0
32	11805.0
33	15318.0
34	21367.0
35	24698.0
36	28996.0
37	37507.0
38	39892.0
39	20931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.879180610404273	36.8215107847194	18.878180276959792	22.421128327916534
2	24.949682022521564	36.5495325751154	17.185246487785086	21.315538914577953
3	20.89813070220194	31.894969005732836	22.515135567277447	24.69176472478778
4	23.337498041515847	33.006986264497804	17.66090704933773	25.994608644648622
5	17.06030524230969	37.88933660617796	17.661710530016027	27.38864762149632
6	19.149756746224643	39.228337156562226	22.80278165010827	18.81912444710486
7	70.3053628317873	17.676976662903698	6.868554578433775	5.149105926875223
8	76.92604362096603	8.104307861656697	5.876657681074414	9.092990836302864
9	68.79843481963867	15.369781895169876	9.813311264397369	6.01847202079408
10	31.119610151174893	34.6565320970444	14.469481795136533	19.754375956644182
11	25.6547363177284	31.600895077475627	24.057416729271203	18.686951875524773
12	24.074691563854618	27.40351201404484	31.60451074052797	16.917285681572572
13	24.957315088965398	29.621520426487546	25.27669865858901	20.14446582595805
14	18.31976120554241	32.716527999292936	30.762864730010403	18.20084606515425
15	17.35156698819285	33.69516746546037	27.745794782999955	21.20747076334682
16	18.58450808904173	30.4916900010847	28.510306648400874	22.4134952614727
17	17.878650313156594	30.521820526520887	27.029893498636092	24.569635661686426
18	20.630973376667725	30.370364418661644	24.33984018769309	24.658822016977545
19	20.150491931045288	34.604305852955	26.24850853899091	18.996693677008803
20	22.20459028511512	34.18850460193559	23.1109164902357	20.495988622713597
21	21.743392375771844	31.41930844418019	24.39005773008674	22.447241449961233
22	19.708175817642026	32.67434526368227	24.564814777616636	23.05266414105907
23	20.741050229594606	32.20310384586026	22.209812909524057	24.84603301502107
24	18.727929390117993	33.97839440456056	24.66324116070819	22.630435044613264
25	22.041081967081396	29.170366025623	23.20492372959661	25.583628277698995
26	23.291297902513687	30.991053242647148	23.073152898355676	22.64449595648349
27	18.61905775820856	29.147868566630642	25.384365069480992	26.848708605679803
28	24.57204610372132	28.973513259439894	24.943254177095177	21.51118645974361
29	20.456618069476974	32.02874853866952	26.631367082200093	20.88326630965342
30	23.427889617824416	28.19373526115131	24.046167999775026	24.33220712124925
31	27.016636067444168	29.782216562147223	22.529598219486815	20.67154915092179
32	23.483329784627006	26.987710763025426	25.885737012739185	23.643222439608383
33	25.057750173752698	29.4202485165738	22.729263168043968	22.79273814162954
34	22.19936766070618	29.56929418239815	25.541445542088326	22.689892614807345
35	25.423735622717615	27.138765130545522	25.10917293716379	22.32832630957307
36	24.076700265550365	28.43196728226678	26.982488138616485	20.508844313566367
37	22.69350827785969	28.844152870233852	23.806730757642107	24.655608094264352
38	25.620990129239868	29.930458747293276	24.069870679784827	20.378680443682033
39	21.562609223154706	28.434377724301672	26.958785458606684	23.044227593936935
40	24.435052648071444	30.26189452709136	23.810346420694447	21.492706404142748
41	23.248311686224728	26.71010818867333	28.707962895262273	21.333617229839668
42	27.308299553666483	26.46826050450552	23.691029539967136	22.53241040186086
43	23.089624252260794	25.547873387514713	27.022260432192258	24.340241928032235
44	22.645299437161786	24.601774888818362	25.64629977060627	27.10662590341359
45	27.04154396847142	26.51687108554257	25.859222150355336	20.582362795630672
46	21.553369195354275	24.749615333625265	31.06939260878124	22.627622862239217
47	23.946536395666026	27.95148583664434	24.1578518140585	23.94412595363113
48	21.77151419951229	25.91827798021027	30.511375277703007	21.79883254257443
49	23.1133269322706	23.701876529124167	28.85058071566024	24.334215822944998
50	20.823808739459338	25.180280977193203	26.638598408304777	27.357311875042683
51	21.089760843976105	23.586978792127496	30.749205558479332	24.574054805417067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3087.0
1	2376.5
2	1666.0
3	1208.0
4	750.0
5	693.5
6	637.0
7	725.5
8	814.0
9	926.0
10	1038.0
11	1065.0
12	1092.0
13	1087.0
14	1082.0
15	1027.0
16	972.0
17	948.5
18	925.0
19	860.5
20	796.0
21	861.5
22	927.0
23	967.5
24	1008.0
25	1273.0
26	1746.5
27	1955.0
28	2262.0
29	2569.0
30	3103.5
31	3638.0
32	4175.0
33	4712.0
34	4910.5
35	5109.0
36	5586.0
37	6063.0
38	7395.5
39	8728.0
40	9151.5
41	9575.0
42	11299.5
43	13024.0
44	18750.5
45	24477.0
46	24859.0
47	25241.0
48	25249.0
49	25257.0
50	23718.0
51	22179.0
52	19603.0
53	17027.0
54	15173.5
55	13320.0
56	12082.0
57	10844.0
58	9951.0
59	9058.0
60	7877.5
61	6697.0
62	6042.5
63	5388.0
64	5223.5
65	5059.0
66	4121.0
67	3183.0
68	2838.5
69	2494.0
70	2135.5
71	1777.0
72	1646.5
73	1516.0
74	1397.5
75	1096.0
76	913.0
77	732.0
78	551.0
79	511.0
80	471.0
81	381.0
82	291.0
83	183.5
84	76.0
85	59.5
86	43.0
87	33.0
88	23.0
89	23.0
90	23.0
91	16.0
92	9.0
93	9.0
94	9.0
95	7.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	248917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.100181988373635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2554995016799	30.9890445409514
2	8.705703453306892	6.807891787222246
3	3.1984957925344455	3.751853027314326
4	1.7199749298755742	2.6900533109430054
5	0.9997225846887297	1.9544667499608304
6	0.638055215921584	1.4968845036698981
7	0.4767433497385104	1.304852621556583
8	0.3976286128206973	1.2437880900059055
9	0.36680468934622457	1.2907917096863615
>10	3.968066415280446	34.1306539930981
>50	0.2219322490162031	5.322256013048526
>100	0.0410985646326302	2.8419111591414006
>500	0.005137320579078775	1.2731151347637968
>1k	0.005137320579078775	4.902437358637618
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	2647	1.0634066777279172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2644	1.0622014567104698	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT	2615	1.0505509868751433	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	2536	1.0188135000823568	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	1761	0.7074647372417312	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	781	0.3137592048755208	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	711	0.28563738113507714	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	595	0.23903550179377062	No Hit
AACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	546	0.2193502251754601	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	536	0.21533282178396815	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	436	0.1751587878690487	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.1707396441384076	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	394	0.15828569362478256	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTAT	350	0.140609118702218	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	335	0.1345830136149801	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	321	0.12895864886689137	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9585524492099776	0.0
2	0.0	0.0	0.0	1.2257097747441918	0.0
3	0.0	0.0	0.0	1.784530586500721	0.0
4	0.0	0.0	0.0	4.371336630282383	0.0
5	0.0	0.0	0.0	4.792360505710739	0.0
6	0.0	0.0	0.0	6.435880233170093	0.0
7	0.0	0.0	0.0	7.776889485250104	0.0
8	0.0	0.0	0.0	8.589610191348923	0.0
9	0.0	0.0	0.0	10.761820205128616	0.0
10	0.0	0.0	0.0	12.384851175291363	0.0
11	0.0	0.0	0.0	14.970050257716428	0.0
12	0.0	0.0	0.0	15.90088262352511	0.0
13	0.0	0.0	0.0	16.421136362723317	0.0
14	0.0	0.0	0.0	16.967101483627072	0.0
15	0.0	0.0	0.0	17.270415439684715	0.0
16	0.0	0.0	0.0	17.877445092139148	0.0
17	0.0	0.0	0.0	18.490902590019967	0.0
18	0.0	0.0	0.0	19.58604675454067	0.0
19	0.0	0.0	0.0	19.91989297637365	0.0
20	0.0	0.0	0.0	20.347746437567544	0.0
21	0.0	0.0	0.0	20.72939975975928	0.0
22	0.0	0.0	0.0	21.13194357958677	0.0
23	0.0	0.0	0.0	21.56060052145896	0.0
24	0.0	0.0	0.0	21.848246604289784	0.0
25	0.0	0.0	0.0	22.12906310135507	0.0
26	0.0	0.0	0.0	22.356448133313513	0.0
27	0.0	0.0	0.0	22.64369247580519	0.0
28	0.0	0.0	0.0	22.90723413828706	0.0
29	0.0	0.0	0.0	23.20773591197066	0.0
30	0.0	0.0	0.0	23.66973730199223	0.0
31	0.0	0.0	0.0	23.938099848543892	0.0
32	0.0	0.0	0.0	24.300067894117316	0.0
33	0.0	0.0	0.0	24.573653065077917	0.0
34	4.0174033914919434E-4	0.0	0.0	24.82233033501127	0.0
35	4.0174033914919434E-4	0.0	0.0	25.092299842919527	0.0
36	4.0174033914919434E-4	0.0	0.0	25.343789295226923	0.0
37	4.0174033914919434E-4	0.0	0.0	25.620186648561567	0.0
38	4.0174033914919434E-4	0.0	0.0	25.884130051382588	0.0
39	4.0174033914919434E-4	0.0	0.0	26.143654310472968	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTGCA	25	3.8819722E-5	45.000004	31
TACGCAG	25	3.8819722E-5	45.000004	25
CTAAACG	35	1.2068085E-7	45.000004	1
ACTACCG	25	3.8819722E-5	45.000004	1
CGGGATG	35	1.2068085E-7	45.000004	6
ACGTAGG	50	2.1827873E-11	45.000004	2
AATACGG	25	3.8819722E-5	45.000004	2
CCCGCTG	25	3.8819722E-5	45.000004	35
GGCTACC	25	3.8819722E-5	45.000004	8
AATCCGG	20	7.022628E-4	45.0	2
CGAAAGA	20	7.022628E-4	45.0	29
GGCAACG	20	7.022628E-4	45.0	28
TAACGGG	80	0.0	45.0	3
TAGCCCG	20	7.022628E-4	45.0	1
ATCGTTC	20	7.022628E-4	45.0	26
CTAACGG	40	6.7775545E-9	45.0	2
AATTGGC	20	7.022628E-4	45.0	41
CGCTAAC	40	6.7775545E-9	45.0	38
GTCACGG	20	7.022628E-4	45.0	2
ACGGAAC	20	7.022628E-4	45.0	5
>>END_MODULE