FastQCFastQC Report
Sat 18 Jun 2016
SRR3548612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356755
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28070.7868144805258511No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT26050.7301929895866911TruSeq Adapter, Index 20 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT15480.4339112275931662No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC13360.37448669254810724No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT13130.36803969110453955No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT8980.25171336070973077No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7470.20938739471065576No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT7280.2040616109094477No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC6510.18247817129402533No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.1735084301551485No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.15921290521506357No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC4890.13706885677846142No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4380.12277333183837648No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT4290.12025059214306737No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3620.10147019663354404No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.10034897899118443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGT253.8853785E-545.00000424
CGGTTAG253.8853785E-545.00000439
TCGGGTA253.8853785E-545.0000045
CGTTAAG253.8853785E-545.0000041
CCGTAGT253.8853785E-545.00000427
CGTAGTG253.8853785E-545.00000428
TACGATT253.8853785E-545.00000411
AAGCACG302.1612777E-645.0000041
ATAACGG302.1612777E-645.0000042
ACCGTAG253.8853785E-545.00000426
CGGTAGT351.208773E-745.00000412
TAGTGCC302.1612777E-645.00000430
CATCCGG302.1612777E-645.0000042
ACATACG502.1827873E-1145.00000417
GGTTAGC253.8853785E-545.00000440
GTAACGG502.1827873E-1145.0000042
GTACGAT253.8853785E-545.00000410
ACTATCG207.026739E-445.01
TAGCGAT207.026739E-445.045
TAAACGG207.026739E-445.02