Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548612_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 356755 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2807 | 0.7868144805258511 | No Hit |
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT | 2605 | 0.7301929895866911 | TruSeq Adapter, Index 20 (95% over 23bp) |
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT | 1548 | 0.4339112275931662 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC | 1336 | 0.37448669254810724 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT | 1313 | 0.36803969110453955 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT | 898 | 0.25171336070973077 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 747 | 0.20938739471065576 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT | 728 | 0.2040616109094477 | No Hit |
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC | 651 | 0.18247817129402533 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 619 | 0.1735084301551485 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 568 | 0.15921290521506357 | No Hit |
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC | 489 | 0.13706885677846142 | No Hit |
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 438 | 0.12277333183837648 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT | 429 | 0.12025059214306737 | No Hit |
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 362 | 0.10147019663354404 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 358 | 0.10034897899118443 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TACCAGT | 25 | 3.8853785E-5 | 45.000004 | 24 |
CGGTTAG | 25 | 3.8853785E-5 | 45.000004 | 39 |
TCGGGTA | 25 | 3.8853785E-5 | 45.000004 | 5 |
CGTTAAG | 25 | 3.8853785E-5 | 45.000004 | 1 |
CCGTAGT | 25 | 3.8853785E-5 | 45.000004 | 27 |
CGTAGTG | 25 | 3.8853785E-5 | 45.000004 | 28 |
TACGATT | 25 | 3.8853785E-5 | 45.000004 | 11 |
AAGCACG | 30 | 2.1612777E-6 | 45.000004 | 1 |
ATAACGG | 30 | 2.1612777E-6 | 45.000004 | 2 |
ACCGTAG | 25 | 3.8853785E-5 | 45.000004 | 26 |
CGGTAGT | 35 | 1.208773E-7 | 45.000004 | 12 |
TAGTGCC | 30 | 2.1612777E-6 | 45.000004 | 30 |
CATCCGG | 30 | 2.1612777E-6 | 45.000004 | 2 |
ACATACG | 50 | 2.1827873E-11 | 45.000004 | 17 |
GGTTAGC | 25 | 3.8853785E-5 | 45.000004 | 40 |
GTAACGG | 50 | 2.1827873E-11 | 45.000004 | 2 |
GTACGAT | 25 | 3.8853785E-5 | 45.000004 | 10 |
ACTATCG | 20 | 7.026739E-4 | 45.0 | 1 |
TAGCGAT | 20 | 7.026739E-4 | 45.0 | 45 |
TAAACGG | 20 | 7.026739E-4 | 45.0 | 2 |