##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356755
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94529298818517	31.0	31.0	33.0	28.0	34.0
2	31.130742386231447	31.0	31.0	34.0	28.0	34.0
3	31.346217992740115	31.0	31.0	34.0	28.0	34.0
4	35.34883603593502	37.0	35.0	37.0	33.0	37.0
5	34.93772476909924	35.0	35.0	37.0	32.0	37.0
6	34.97411669072613	35.0	35.0	37.0	32.0	37.0
7	33.94091743633586	35.0	35.0	37.0	30.0	37.0
8	34.700845117797925	37.0	35.0	37.0	32.0	37.0
9	36.01385544701546	39.0	35.0	39.0	32.0	39.0
10	35.626962481254644	37.0	35.0	39.0	30.0	39.0
11	36.06732351333548	37.0	35.0	39.0	31.0	39.0
12	36.089515213521885	37.0	35.0	39.0	31.0	39.0
13	36.0623425039593	37.0	35.0	39.0	31.0	39.0
14	37.12855881487295	39.0	36.0	40.0	32.0	41.0
15	37.107533181034604	39.0	36.0	40.0	32.0	41.0
16	37.00998444310521	39.0	36.0	40.0	31.0	41.0
17	36.9254446328713	38.0	36.0	40.0	31.0	41.0
18	36.7501534666648	38.0	35.0	40.0	31.0	41.0
19	36.72053650264187	38.0	35.0	40.0	31.0	41.0
20	36.73280822973749	38.0	35.0	40.0	31.0	41.0
21	36.74536867037603	38.0	35.0	40.0	31.0	41.0
22	36.74371487435355	39.0	35.0	40.0	31.0	41.0
23	36.61591007834508	38.0	35.0	40.0	31.0	41.0
24	36.302369973791535	38.0	35.0	40.0	30.0	41.0
25	36.41101316029208	38.0	35.0	40.0	30.0	41.0
26	36.3265854718224	38.0	35.0	40.0	30.0	41.0
27	36.25440428305139	38.0	35.0	40.0	30.0	41.0
28	36.09452985942734	38.0	35.0	40.0	30.0	41.0
29	35.80859693627279	38.0	34.0	40.0	29.0	41.0
30	35.85361102156943	38.0	34.0	40.0	29.0	41.0
31	35.646931367465065	38.0	34.0	40.0	28.0	41.0
32	35.54735602864711	38.0	34.0	40.0	27.0	41.0
33	35.54647026670965	38.0	34.0	40.0	27.0	41.0
34	35.46946223598828	38.0	34.0	40.0	27.0	41.0
35	35.510944485711484	38.0	34.0	40.0	27.0	41.0
36	35.24185505458929	38.0	34.0	40.0	26.0	41.0
37	35.248882286162775	38.0	34.0	40.0	27.0	41.0
38	35.057431570685765	38.0	34.0	40.0	26.0	41.0
39	34.97483427001724	38.0	34.0	40.0	25.0	41.0
40	34.7606088211798	38.0	33.0	40.0	25.0	41.0
41	34.74091743633586	38.0	33.0	40.0	25.0	41.0
42	34.69738055528304	37.0	33.0	40.0	25.0	41.0
43	34.647542431080154	37.0	33.0	40.0	24.0	41.0
44	34.54532662471444	37.0	33.0	40.0	24.0	41.0
45	34.24543734495662	37.0	33.0	40.0	24.0	41.0
46	34.10276520301047	37.0	33.0	40.0	23.0	41.0
47	33.89822707460302	36.0	33.0	40.0	23.0	41.0
48	33.80559487603537	36.0	33.0	39.0	23.0	41.0
49	33.60188364563916	36.0	33.0	39.0	22.0	40.0
50	33.49202674104077	36.0	32.0	39.0	22.0	40.0
51	31.45638042914605	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	9.0
12	2.0
13	7.0
14	21.0
15	39.0
16	81.0
17	163.0
18	360.0
19	640.0
20	951.0
21	1565.0
22	2167.0
23	2817.0
24	3254.0
25	3845.0
26	4483.0
27	5095.0
28	6229.0
29	7699.0
30	9601.0
31	12374.0
32	15825.0
33	20855.0
34	28810.0
35	35177.0
36	42382.0
37	55358.0
38	63943.0
39	32998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.91756247284551	32.35778054967695	19.052010483384958	26.672646494092582
2	28.263934633011452	31.22731286176788	17.49211643845216	23.016636066768513
3	20.98695182968704	31.832209779820886	18.972684335188013	28.208154055304057
4	21.880001681826464	35.097756163193225	17.45287382096957	25.569368334010733
5	17.76457232554554	36.877969474849685	17.209849896988132	28.14760830261664
6	18.717046712730028	39.13554119774075	19.588232820843434	22.559179268685792
7	78.13064988577595	11.02801642583846	5.898445712043279	4.942887976342307
8	81.5430757802974	7.104875895222211	5.012683774579193	6.339364549901193
9	75.14232456447702	10.83376546929966	8.801558492522881	5.222351473700439
10	30.19046684699584	36.38883827837031	13.322588330927388	20.098106543706464
11	25.599921514765033	30.55149892783563	23.92398144384802	19.92459811355132
12	23.400653109276675	28.252161847766676	29.55725918347325	18.7899258594834
13	23.539403792518677	31.042592255189135	25.739232806828216	19.678771145463976
14	19.243178091407266	32.80991156395846	28.4929433364634	19.453967008170874
15	17.59470785272806	31.92190719120965	26.63396448543118	23.849420470631106
16	19.277375229499235	29.947442923014396	26.811116872923996	23.964064974562373
17	17.902762399966363	30.179815279393424	27.262406973973736	24.655015346666477
18	23.80345054729436	27.881599416966825	24.73518240809519	23.579767627643623
19	20.42494148645429	32.668918445431736	23.876329694047733	23.029810374066237
20	25.314011015963334	29.948283836246166	23.164356491149388	21.573348656641112
21	21.173354262729323	33.295959411921345	23.262182730445264	22.268503594904065
22	22.5090047791902	26.93501142240473	23.615646592199127	26.94033720620594
23	20.658154756065088	34.11556950848622	21.41161301173074	23.81466272371796
24	18.441227172709564	32.59323625457247	26.42878165687937	22.5367549158386
25	21.797872489523623	30.448066600327955	22.701573909265463	25.052487000882962
26	25.370912811313083	28.994127622598143	21.82309988647671	23.811859679612056
27	20.977982088548163	27.345937688329524	23.58649493349778	28.08958528962453
28	20.44540370842735	31.491639920954157	24.329021317150428	23.733935053468066
29	19.894044932797016	32.89091953861894	23.802889938473182	23.41214559011086
30	22.63037658897563	26.03018878502053	25.85051365783241	25.488920968171435
31	25.72914184804698	29.895586607055264	20.06194727474037	24.31332427015739
32	21.170270914212836	26.440834746534737	25.13882075934465	27.25007357990778
33	19.209541562136483	25.680649185014925	24.415635380022703	30.694173872825885
34	17.49043461198862	29.054953679696148	25.44351165365587	28.01110005465936
35	19.253549354599095	26.094378495045618	27.562893302126106	27.089178848229178
36	21.254081932979215	29.85438185869855	25.35689759078359	23.534638617538647
37	19.049487743689646	29.72880548275427	20.660957800170987	30.560748973385092
38	20.048492663032054	30.684643522865834	23.677593866939496	25.58926994716262
39	19.513671847626522	25.46677691973483	24.27884682765483	30.740704404983816
40	23.677033258118314	26.01028717186865	24.59867416013791	25.714005409875124
41	20.402236829196507	24.57344676318482	27.161497386161372	27.8628190214573
42	24.731538450757522	26.02430239239815	23.131841179520958	26.112317977323375
43	21.459825370352203	24.730136928704574	25.97468851172373	27.835349189219492
44	23.98312567448249	23.426721419461536	25.06902496110776	27.521127944948216
45	25.98786281902146	24.059929082984123	25.495087665204412	24.45712043279001
46	21.570825916945804	23.18425810430127	28.472200810079745	26.772715168673177
47	21.651833891606284	27.77536404535325	25.02109290689689	25.55170915614357
48	21.147566256955052	27.04152709842889	28.396518619220473	23.41438802539558
49	23.429804767978023	24.859076957575926	26.31890232792813	25.392215946517922
50	20.73411725133495	25.455845047721827	26.045885832013564	27.76415186892966
51	20.749814298327983	22.33914030637272	29.17408305419686	27.73696234110244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3338.0
1	2671.5
2	2005.0
3	1422.0
4	839.0
5	809.5
6	780.0
7	848.0
8	916.0
9	1015.0
10	1114.0
11	1248.5
12	1383.0
13	1330.5
14	1278.0
15	1257.5
16	1237.0
17	1243.5
18	1250.0
19	1118.5
20	987.0
21	1035.0
22	1083.0
23	1075.0
24	1067.0
25	1214.5
26	1573.5
27	1785.0
28	2489.5
29	3194.0
30	3349.0
31	3504.0
32	4096.0
33	4688.0
34	5434.5
35	6181.0
36	7227.0
37	8273.0
38	9197.5
39	10122.0
40	10993.0
41	11864.0
42	14270.0
43	16676.0
44	21113.5
45	25551.0
46	28382.0
47	31213.0
48	33888.5
49	36564.0
50	36368.5
51	36173.0
52	33396.5
53	30620.0
54	28248.5
55	25877.0
56	22161.0
57	18445.0
58	16779.0
59	15113.0
60	13806.5
61	12500.0
62	11013.0
63	9526.0
64	8398.5
65	7271.0
66	6350.5
67	5430.0
68	4779.0
69	4128.0
70	3826.5
71	3525.0
72	3593.0
73	3661.0
74	3099.5
75	2052.5
76	1567.0
77	1270.0
78	973.0
79	734.5
80	496.0
81	387.5
82	279.0
83	225.5
84	172.0
85	133.5
86	95.0
87	72.0
88	49.0
89	41.5
90	34.0
91	24.5
92	15.0
93	11.0
94	7.0
95	5.5
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	356755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.197967753118625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0979052384989	29.42281328400915
2	8.961249627569092	6.666805493479227
3	3.0833968683672137	3.440882918387717
4	1.4325121426607814	2.1314616195458647
5	0.8886392816042582	1.6527787670634855
6	0.6318711025650584	1.4102592538405534
7	0.5085168704903397	1.324105590529152
8	0.3786986739517756	1.1269456849445547
9	0.35768315021526537	1.1974577658787195
>10	4.253112138966552	35.72685050971429
>50	0.33517982026662024	7.83476243415305
>100	0.06298681186218368	4.210114428463318
>500	0.004499057990155977	1.1745588881846452
>1k	0.0037492149917966477	2.6802033618062824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2807	0.7868144805258511	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	2605	0.7301929895866911	TruSeq Adapter, Index 20 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	1548	0.4339112275931662	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	1336	0.37448669254810724	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	1313	0.36803969110453955	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	898	0.25171336070973077	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	747	0.20938739471065576	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	728	0.2040616109094477	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	651	0.18247817129402533	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	619	0.1735084301551485	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.15921290521506357	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	489	0.13706885677846142	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	438	0.12277333183837648	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	429	0.12025059214306737	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	362	0.10147019663354404	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	358	0.10034897899118443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.803044105899006E-4	0.0	0.0	0.896974113887682	0.0
2	2.803044105899006E-4	0.0	0.0	1.1327101231937884	0.0
3	2.803044105899006E-4	0.0	0.0	1.5060755980995362	0.0
4	2.803044105899006E-4	0.0	0.0	3.3490770977281326	0.0
5	2.803044105899006E-4	0.0	0.0	3.6829196507407045	0.0
6	2.803044105899006E-4	0.0	0.0	5.280654791103138	0.0
7	2.803044105899006E-4	0.0	0.0	6.559403512214264	0.0
8	2.803044105899006E-4	0.0	0.0	7.572143347675576	0.0
9	2.803044105899006E-4	0.0	0.0	9.046824851789044	0.0
10	2.803044105899006E-4	0.0	0.0	10.91253100867542	0.0
11	2.803044105899006E-4	0.0	0.0	12.744600636291013	0.0
12	2.803044105899006E-4	0.0	0.0	13.651385404549341	0.0
13	2.803044105899006E-4	0.0	0.0	14.081092065983658	0.0
14	2.803044105899006E-4	0.0	0.0	14.493139549550811	0.0
15	2.803044105899006E-4	0.0	0.0	14.77848943953133	0.0
16	2.803044105899006E-4	0.0	0.0	15.387871228153776	0.0
17	2.803044105899006E-4	0.0	0.0	16.092836820787376	0.0
18	2.803044105899006E-4	0.0	0.0	17.03970511976006	0.0
19	2.803044105899006E-4	0.0	0.0	17.386441675659768	0.0
20	2.803044105899006E-4	0.0	0.0	17.777466328432677	0.0
21	2.803044105899006E-4	0.0	0.0	18.190915334052782	0.0
22	2.803044105899006E-4	0.0	0.0	18.624826561645946	0.0
23	2.803044105899006E-4	0.0	0.0	19.09910162436406	0.0
24	2.803044105899006E-4	0.0	0.0	19.423974436237753	0.0
25	2.803044105899006E-4	0.0	0.0	19.711286457092402	0.0
26	2.803044105899006E-4	0.0	0.0	19.978416560384577	0.0
27	2.803044105899006E-4	0.0	0.0	20.27974380176872	0.0
28	2.803044105899006E-4	0.0	0.0	20.600692351894157	0.0
29	2.803044105899006E-4	0.0	0.0	20.93173186080083	0.0
30	2.803044105899006E-4	0.0	0.0	21.448893498339196	0.0
31	2.803044105899006E-4	0.0	0.0	21.765637482305785	0.0
32	2.803044105899006E-4	0.0	0.0	22.11041190733136	0.0
33	2.803044105899006E-4	0.0	0.0	22.47761068520413	0.0
34	2.803044105899006E-4	0.0	0.0	22.802483497077827	0.0
35	2.803044105899006E-4	0.0	0.0	23.19238693220838	0.0
36	2.803044105899006E-4	0.0	0.0	23.47016860310297	0.0
37	2.803044105899006E-4	0.0	0.0	23.759162450421158	0.0
38	2.803044105899006E-4	0.0	0.0	24.044512340401678	0.0
39	2.803044105899006E-4	0.0	0.0	24.40274137713557	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAGT	25	3.8853785E-5	45.000004	24
CGGTTAG	25	3.8853785E-5	45.000004	39
TCGGGTA	25	3.8853785E-5	45.000004	5
CGTTAAG	25	3.8853785E-5	45.000004	1
CCGTAGT	25	3.8853785E-5	45.000004	27
CGTAGTG	25	3.8853785E-5	45.000004	28
TACGATT	25	3.8853785E-5	45.000004	11
AAGCACG	30	2.1612777E-6	45.000004	1
ATAACGG	30	2.1612777E-6	45.000004	2
ACCGTAG	25	3.8853785E-5	45.000004	26
CGGTAGT	35	1.208773E-7	45.000004	12
TAGTGCC	30	2.1612777E-6	45.000004	30
CATCCGG	30	2.1612777E-6	45.000004	2
ACATACG	50	2.1827873E-11	45.000004	17
GGTTAGC	25	3.8853785E-5	45.000004	40
GTAACGG	50	2.1827873E-11	45.000004	2
GTACGAT	25	3.8853785E-5	45.000004	10
ACTATCG	20	7.026739E-4	45.0	1
TAGCGAT	20	7.026739E-4	45.0	45
TAAACGG	20	7.026739E-4	45.0	2
>>END_MODULE