FastQCFastQC Report
Sat 18 Jun 2016
SRR3548611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415763
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35500.8538518338572696No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT28140.6768279043589737TruSeq Adapter, Index 20 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT17030.40960835860814937No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC15160.3646308113035551No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT15150.3643902896602151No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT9670.23258442910985347No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8450.20324078862236417No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT7800.18760688180525925No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7630.18351801386847796No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7170.17245401827483445No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC6690.16090897939451082No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5460.13132481726368148No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4650.11184256415313532No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT4410.1060700447129735No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.10222169841953227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG302.1621472E-645.0000041
TTTACGC302.1621472E-645.0000041
CGGCCTA253.886495E-545.00000442
GACGTAT253.886495E-545.00000413
TACGGGT253.886495E-545.0000044
CGCTATC253.886495E-545.00000428
TACGAAG302.1621472E-645.0000041
ACGGACT253.886495E-545.0000045
CTCGCGG253.886495E-545.0000042
GTATAAT253.886495E-545.00000422
ATTGCCA253.886495E-545.00000421
TATACGG302.1621472E-645.0000042
CCGTTCG253.886495E-545.00000417
CGGGTAC453.8380676E-1045.06
AGTCAAC207.028087E-445.045
CGCATTG207.028087E-445.01
TTATGTC207.028087E-445.039
ATTACGG453.8380676E-1045.02
CACGCTA207.028087E-445.045
ATTCGCG207.028087E-445.01