##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548611_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415763 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95287459442038 31.0 31.0 33.0 28.0 34.0 2 31.15206740378533 31.0 31.0 34.0 28.0 34.0 3 31.365782909975152 31.0 31.0 34.0 28.0 34.0 4 35.362826898978504 37.0 35.0 37.0 33.0 37.0 5 34.978341026017226 35.0 35.0 37.0 32.0 37.0 6 35.00298006316098 35.0 35.0 37.0 32.0 37.0 7 32.7432527666002 35.0 35.0 37.0 26.0 37.0 8 34.123264936995355 37.0 35.0 37.0 28.0 37.0 9 34.358555234592785 38.0 35.0 39.0 28.0 39.0 10 34.83730635001191 37.0 34.0 39.0 27.0 39.0 11 35.80677934303918 37.0 35.0 39.0 30.0 39.0 12 36.027739361126415 37.0 35.0 39.0 32.0 39.0 13 36.098933767555074 37.0 35.0 39.0 32.0 39.0 14 37.16703506565038 39.0 36.0 40.0 32.0 41.0 15 37.16240502401609 39.0 36.0 40.0 32.0 41.0 16 37.10495161907144 39.0 36.0 40.0 32.0 41.0 17 37.02031205278007 39.0 36.0 40.0 32.0 41.0 18 36.872088184855315 38.0 36.0 40.0 31.0 41.0 19 36.814476035626065 39.0 35.0 40.0 31.0 41.0 20 36.794055748106494 39.0 35.0 40.0 31.0 41.0 21 36.82327672255588 39.0 35.0 40.0 31.0 41.0 22 36.8430596277206 39.0 35.0 40.0 31.0 41.0 23 36.723371247561715 38.0 35.0 40.0 31.0 41.0 24 36.415000853851836 38.0 35.0 40.0 30.0 41.0 25 36.531247369294526 38.0 35.0 40.0 30.0 41.0 26 36.426954779525836 38.0 35.0 40.0 30.0 41.0 27 36.368450294999796 38.0 35.0 40.0 30.0 41.0 28 36.2388812857325 38.0 35.0 40.0 30.0 41.0 29 35.85190841897908 38.0 34.0 40.0 29.0 41.0 30 35.92268672296477 38.0 35.0 40.0 29.0 41.0 31 35.76595079408221 38.0 34.0 40.0 29.0 41.0 32 35.745441513554596 38.0 34.0 40.0 29.0 41.0 33 35.73395179465224 38.0 35.0 40.0 29.0 41.0 34 35.618294557235735 38.0 34.0 40.0 28.0 41.0 35 35.60213390801972 38.0 34.0 40.0 28.0 41.0 36 35.34706551569043 38.0 34.0 40.0 27.0 41.0 37 35.35525287242972 38.0 34.0 40.0 27.0 41.0 38 35.17632882194904 38.0 34.0 40.0 26.0 41.0 39 35.1294824214757 38.0 34.0 40.0 26.0 41.0 40 34.92039695692017 38.0 34.0 40.0 25.0 41.0 41 34.85167511298504 38.0 33.0 40.0 25.0 41.0 42 34.81676339645423 38.0 33.0 40.0 25.0 41.0 43 34.75097591656785 38.0 33.0 40.0 25.0 41.0 44 34.61423214667972 37.0 33.0 40.0 24.0 41.0 45 34.30176326416733 37.0 33.0 40.0 24.0 41.0 46 34.20517698785125 37.0 33.0 40.0 23.0 41.0 47 34.019268188847974 37.0 33.0 40.0 23.0 41.0 48 33.95150843148621 36.0 33.0 40.0 23.0 41.0 49 33.74137669778215 36.0 33.0 39.0 23.0 41.0 50 33.555739688235846 36.0 32.0 39.0 22.0 40.0 51 31.616134672878538 35.0 29.0 38.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 9.0 11 12.0 12 2.0 13 16.0 14 28.0 15 70.0 16 97.0 17 186.0 18 360.0 19 674.0 20 1178.0 21 1639.0 22 2274.0 23 3082.0 24 3774.0 25 4328.0 26 4862.0 27 5795.0 28 7269.0 29 8907.0 30 11450.0 31 14479.0 32 18657.0 33 24569.0 34 34767.0 35 41701.0 36 51301.0 37 67139.0 38 71086.0 39 36048.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.056075215928306 32.27896662281155 19.104393608858892 26.56056455240125 2 28.101586721281112 31.19493557627783 17.391638986634213 23.311838715806843 3 21.19380512455413 31.716867542325794 18.998083042502582 28.09124429061749 4 21.675569976164304 35.165947907822485 17.485923470823522 25.672558645189685 5 17.728850330596998 36.97563275231322 17.184549851718405 28.11096706537138 6 18.671214129203417 38.955847441932065 19.742497528640115 22.630440900224407 7 75.27774236764695 14.359142107402533 5.646245577408283 4.7168699475422295 8 81.51470910109846 7.211319910622158 5.0095847874871025 6.264386200792278 9 71.94627708574356 14.609525138119553 8.379533532324906 5.06466424381198 10 30.204707970646737 36.605229421569504 13.189485355839745 20.000577251944016 11 25.634315703898615 30.513297239052058 23.893901092689827 19.958485964359504 12 23.36643712884504 28.320461416720583 29.63948210879756 18.673619345636816 13 23.610326075191875 31.048217373840387 25.67472333997975 19.666733210987992 14 19.294886750384233 32.9430468800735 28.40825181653971 19.35381455300255 15 17.565536134769086 31.961237531959313 26.57403376442829 23.899192568843308 16 19.18857618402792 29.999061965590972 26.842696440039155 23.96966541034195 17 17.821451163282926 30.324968792316774 27.170767961555022 24.682812082845274 18 23.673342745746975 27.898586454302087 24.918042250031867 23.510028549919067 19 20.319749472656298 32.780935292462296 23.796730348780436 23.102584886100978 20 25.407022750942243 29.98583327520727 23.2317450085746 21.375398965275892 21 21.30636925363729 33.342793851304705 23.189653720990083 22.16118317406792 22 22.50488860240089 26.969932389366058 23.706534732527906 26.81864427570515 23 20.631706044068377 34.30006999179821 21.448277023207936 23.61994694092548 24 18.509583584878886 32.45214220601641 26.424910345557446 22.61336386354726 25 21.642377989383373 30.87407970406217 22.527738158518194 24.95580414803626 26 25.16241223966539 29.03721591387401 21.94543525999187 23.85493658646873 27 21.028807277222842 27.53443668628521 23.61778224613542 27.818973790356527 28 20.410185610552166 31.454939472728455 24.363880383776333 23.770994532943046 29 19.826199060522463 33.014722329788846 23.81597208024764 23.34310652944105 30 22.591957437289995 26.083850655301216 26.121612553305606 25.20257935410318 31 25.748082441198473 29.999543008877655 20.067442268792558 24.184932281131317 32 21.28977326024682 26.584616716735255 25.069089842049436 27.056520180968484 33 19.12772420826288 25.836834927590957 24.489432681599855 30.54600818254631 34 17.592715080466515 29.048279909467656 25.478217157370906 27.880787852694926 35 19.322065696081662 26.129068724249148 27.429809771432286 27.1190558082369 36 21.28111448108658 29.898523918674826 25.312016701822916 23.508344898415682 37 18.952864973554647 29.9608190242999 20.697127931056876 30.389188071088576 38 20.245428284864214 30.74660323309193 23.513636374569167 25.494332107474694 39 19.523141789913964 25.71513097606088 24.120713002359516 30.641014231665636 40 23.78205852853669 26.15312088858316 24.551006222294912 25.513814360585236 41 20.29112739709883 24.85310140633005 27.06133061383529 27.794440582735835 42 24.70494007403256 25.942183407373914 23.175943987319698 26.176932531273827 43 21.59860305029548 24.885571828180957 25.9643113985612 27.551513722962362 44 24.10026866267561 23.3686018236351 25.07149505848284 27.459634455206448 45 26.21325129941818 24.272001116020427 25.306725225669428 24.208022358891963 46 21.72223117497228 23.23583387651138 28.53789298230001 26.50404196621633 47 21.628908777356333 27.884395677345026 25.046240285932132 25.440455259366512 48 21.194045646197473 27.163071268968135 28.450824147410902 23.192058937423486 49 23.457594831670928 25.000300652054175 26.347702898045284 25.194401618229616 50 20.703862537070396 25.659329954805983 26.125701421242393 27.511106086881227 51 20.723104268537604 22.550828236278843 29.16493290648759 27.561134588695964 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4164.0 1 3287.0 2 2410.0 3 1672.5 4 935.0 5 913.0 6 891.0 7 980.0 8 1069.0 9 1172.0 10 1275.0 11 1424.0 12 1573.0 13 1531.0 14 1489.0 15 1492.5 16 1496.0 17 1481.5 18 1467.0 19 1331.0 20 1195.0 21 1242.0 22 1289.0 23 1344.0 24 1399.0 25 1461.5 26 1879.0 27 2234.0 28 3099.5 29 3965.0 30 4127.0 31 4289.0 32 4926.0 33 5563.0 34 6576.5 35 7590.0 36 8734.5 37 9879.0 38 10841.5 39 11804.0 40 12860.5 41 13917.0 42 17000.5 43 20084.0 44 25361.0 45 30638.0 46 33608.5 47 36579.0 48 39582.0 49 42585.0 50 42052.5 51 41520.0 52 38432.5 53 35345.0 54 32368.0 55 29391.0 56 25292.5 57 21194.0 58 19407.0 59 17620.0 60 15964.5 61 14309.0 62 12494.5 63 10680.0 64 9482.0 65 8284.0 66 7161.5 67 6039.0 68 5404.0 69 4769.0 70 4515.5 71 4262.0 72 4215.5 73 4169.0 74 3488.0 75 2234.0 76 1661.0 77 1361.0 78 1061.0 79 793.5 80 526.0 81 427.5 82 329.0 83 274.5 84 220.0 85 166.0 86 112.0 87 94.0 88 76.0 89 59.0 90 42.0 91 35.5 92 29.0 93 18.5 94 8.0 95 4.0 96 0.0 97 1.5 98 3.0 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 415763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.96995575709806 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33793014189835 28.591798556915226 2 9.598123876172883 7.096844301065159 3 3.6689709229806287 4.069250780900192 4 1.9679318132437889 2.9101740827443456 5 1.0945723472100546 2.0233145624649347 6 0.7500353542719205 1.663726431821536 7 0.542850589966284 1.4048413605637668 8 0.39187653836439795 1.1590126628461226 9 0.2950403364760367 0.9816865367470562 >10 3.885503994043577 32.661152759096865 >50 0.3980029218952397 9.418744773808035 >100 0.06133159780025006 4.06713590368472 >500 0.004567246644699472 1.2753067743776239 >1k 0.003262319031928195 2.6770105129644164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3550 0.8538518338572696 No Hit CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT 2814 0.6768279043589737 TruSeq Adapter, Index 20 (95% over 23bp) AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT 1703 0.40960835860814937 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC 1516 0.3646308113035551 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT 1515 0.3643902896602151 No Hit AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT 967 0.23258442910985347 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 845 0.20324078862236417 No Hit AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT 780 0.18760688180525925 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 763 0.18351801386847796 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 717 0.17245401827483445 No Hit CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC 669 0.16090897939451082 No Hit CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC 546 0.13132481726368148 No Hit AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 465 0.11184256415313532 No Hit AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT 441 0.1060700447129735 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 425 0.10222169841953227 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.215649300202279E-4 0.0 0.0 0.8509655741371888 0.0 2 7.215649300202279E-4 0.0 0.0 1.0736886158700991 0.0 3 7.215649300202279E-4 0.0 0.0 1.4010385724559424 0.0 4 7.215649300202279E-4 0.0 0.0 3.1015265908702796 0.0 5 7.215649300202279E-4 0.0 0.0 3.4219014197992608 0.0 6 7.215649300202279E-4 0.0 0.0 4.851562067812672 0.0 7 7.215649300202279E-4 0.0 0.0 6.0010150013348955 0.0 8 7.215649300202279E-4 0.0 0.0 6.96719044263198 0.0 9 7.215649300202279E-4 0.0 0.0 8.373279969598064 0.0 10 7.215649300202279E-4 0.0 0.0 10.22529662331665 0.0 11 7.215649300202279E-4 0.0 0.0 12.058552588854708 0.0 12 7.215649300202279E-4 0.0 0.0 12.969648573826916 0.0 13 7.215649300202279E-4 0.0 0.0 13.411486832642636 0.0 14 7.215649300202279E-4 0.0 0.0 13.823259886040846 0.0 15 7.215649300202279E-4 0.0 0.0 14.111404814762256 0.0 16 7.215649300202279E-4 0.0 0.0 14.713189966399126 0.0 17 7.215649300202279E-4 0.0 0.0 15.423450379182372 0.0 18 7.215649300202279E-4 0.0 0.0 16.362446874782027 0.0 19 7.215649300202279E-4 0.0 0.0 16.69701248066807 0.0 20 9.620865733603038E-4 0.0 0.0 17.06573215990841 0.0 21 9.620865733603038E-4 0.0 0.0 17.469567999076396 0.0 22 9.620865733603038E-4 0.0 0.0 17.89048087492153 0.0 23 0.0019241731467206076 0.0 0.0 18.358535992861317 0.0 24 0.0019241731467206076 0.0 0.0 18.67626508371356 0.0 25 0.0019241731467206076 0.0 0.0 18.955751233274725 0.0 26 0.0021646947900606835 0.0 0.0 19.21960347601879 0.0 27 0.0021646947900606835 0.0 0.0 19.521458138410583 0.0 28 0.0021646947900606835 0.0 0.0 19.82692062545248 0.0 29 0.0021646947900606835 0.0 0.0 20.14007980508126 0.0 30 0.0021646947900606835 0.0 0.0 20.67788619958967 0.0 31 0.0021646947900606835 0.0 0.0 20.971803647751244 0.0 32 0.0021646947900606835 0.0 0.0 21.31310385965081 0.0 33 0.0021646947900606835 0.0 0.0 21.681583017247807 0.0 34 0.0021646947900606835 0.0 0.0 22.001476802890107 0.0 35 0.0024052164334007597 0.0 0.0 22.368512830627065 0.0 36 0.0024052164334007597 0.0 0.0 22.64318854732143 0.0 37 0.0024052164334007597 0.0 0.0 22.909446006498897 0.0 38 0.0024052164334007597 0.0 0.0 23.202401368087106 0.0 39 0.0024052164334007597 0.0 0.0 23.5564492270837 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGCG 30 2.1621472E-6 45.000004 1 TTTACGC 30 2.1621472E-6 45.000004 1 CGGCCTA 25 3.886495E-5 45.000004 42 GACGTAT 25 3.886495E-5 45.000004 13 TACGGGT 25 3.886495E-5 45.000004 4 CGCTATC 25 3.886495E-5 45.000004 28 TACGAAG 30 2.1621472E-6 45.000004 1 ACGGACT 25 3.886495E-5 45.000004 5 CTCGCGG 25 3.886495E-5 45.000004 2 GTATAAT 25 3.886495E-5 45.000004 22 ATTGCCA 25 3.886495E-5 45.000004 21 TATACGG 30 2.1621472E-6 45.000004 2 CCGTTCG 25 3.886495E-5 45.000004 17 CGGGTAC 45 3.8380676E-10 45.0 6 AGTCAAC 20 7.028087E-4 45.0 45 CGCATTG 20 7.028087E-4 45.0 1 TTATGTC 20 7.028087E-4 45.0 39 ATTACGG 45 3.8380676E-10 45.0 2 CACGCTA 20 7.028087E-4 45.0 45 ATTCGCG 20 7.028087E-4 45.0 1 >>END_MODULE