##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548610_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 691442 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.592081476103562 31.0 31.0 34.0 30.0 34.0 2 31.79711096520026 33.0 31.0 34.0 30.0 34.0 3 31.97702627263024 33.0 31.0 34.0 30.0 34.0 4 35.70612141003873 37.0 35.0 37.0 33.0 37.0 5 35.446173359443016 37.0 35.0 37.0 33.0 37.0 6 34.089011081189746 37.0 35.0 37.0 32.0 37.0 7 34.91887822839804 37.0 35.0 37.0 32.0 37.0 8 35.593724419401774 37.0 35.0 37.0 32.0 37.0 9 37.262763904998536 39.0 37.0 39.0 34.0 39.0 10 36.665342863175795 38.0 37.0 39.0 32.0 39.0 11 36.67305428365648 39.0 35.0 39.0 32.0 39.0 12 36.712088649517966 39.0 35.0 39.0 32.0 39.0 13 36.70324770551977 39.0 35.0 39.0 32.0 39.0 14 37.859545703038 40.0 37.0 41.0 33.0 41.0 15 37.86029920080065 40.0 37.0 41.0 33.0 41.0 16 37.70818955169052 39.0 37.0 41.0 32.0 41.0 17 37.619934860769234 39.0 37.0 41.0 32.0 41.0 18 37.40920279647462 39.0 36.0 40.0 32.0 41.0 19 37.30320692118789 39.0 35.0 40.0 32.0 41.0 20 37.19148243815099 39.0 35.0 40.0 32.0 41.0 21 37.16512014022868 39.0 35.0 40.0 32.0 41.0 22 37.13067473482953 39.0 35.0 40.0 32.0 41.0 23 36.90815715562549 39.0 35.0 40.0 31.0 41.0 24 36.77388558982532 39.0 35.0 40.0 31.0 41.0 25 36.823111699896735 39.0 35.0 40.0 31.0 41.0 26 36.72799309269613 39.0 35.0 40.0 31.0 41.0 27 36.67899838308925 39.0 35.0 40.0 31.0 41.0 28 36.58970528258335 39.0 35.0 40.0 31.0 41.0 29 36.41606382024812 38.0 35.0 40.0 30.0 41.0 30 36.40213495853593 38.0 35.0 40.0 30.0 41.0 31 36.28439840217979 38.0 35.0 40.0 30.0 41.0 32 36.21043124369072 38.0 35.0 40.0 30.0 41.0 33 36.10923982054894 38.0 35.0 40.0 30.0 41.0 34 35.98845456307254 38.0 35.0 40.0 30.0 41.0 35 35.84553006615161 38.0 35.0 40.0 29.0 41.0 36 35.783666598210694 38.0 35.0 40.0 29.0 41.0 37 35.73784641372668 38.0 35.0 40.0 29.0 41.0 38 35.65519161404716 38.0 35.0 40.0 28.0 41.0 39 35.59126578946607 38.0 35.0 40.0 28.0 41.0 40 35.413923365951156 38.0 34.0 40.0 27.0 41.0 41 35.27829087616893 38.0 34.0 40.0 26.0 41.0 42 35.13141666256895 38.0 34.0 40.0 26.0 41.0 43 35.043153294130235 38.0 34.0 40.0 25.0 41.0 44 34.91851088016059 38.0 34.0 40.0 25.0 41.0 45 34.71189340537601 37.0 33.0 40.0 24.0 41.0 46 34.558046806528964 37.0 33.0 40.0 23.0 41.0 47 34.467386997029394 37.0 33.0 40.0 23.0 41.0 48 34.32800003471007 37.0 33.0 40.0 23.0 41.0 49 34.14247471226798 37.0 33.0 40.0 23.0 41.0 50 34.019421730239124 37.0 33.0 40.0 23.0 41.0 51 32.932039129818556 35.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 8.0 10 8.0 11 12.0 12 15.0 13 24.0 14 24.0 15 55.0 16 139.0 17 315.0 18 577.0 19 1101.0 20 1710.0 21 2572.0 22 3582.0 23 4662.0 24 5781.0 25 6643.0 26 7402.0 27 8639.0 28 10332.0 29 12635.0 30 15655.0 31 19537.0 32 25075.0 33 33108.0 34 47264.0 35 69653.0 36 67581.0 37 90367.0 38 128127.0 39 128823.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.357250499680376 34.81101813311891 18.404435946905163 25.427295420295554 2 21.890339319856185 35.053120869140145 18.202683666887463 24.85385614411621 3 19.717344332568747 36.68420489354132 18.071653153843705 25.52679762004622 4 18.67792237092916 37.20355431113528 18.303487494251144 25.81503582368442 5 17.56908026992864 39.42702352474973 15.714550171959472 27.28934603336216 6 17.57775778734876 43.51500198136648 16.77624442831069 22.130995802974073 7 80.15885641890425 12.750310221247767 3.952753810153274 3.138079549694696 8 81.51616476870078 12.320773108952016 3.186239771376341 2.9768223509708696 9 74.68638005790797 16.178508103355018 5.430968902670072 3.704142936066944 10 29.48837357290995 46.84210678552937 11.771197005677989 11.89832263588269 11 19.239502373301015 33.61626860965924 30.864483210450043 16.27974580658971 12 21.385452431295757 30.366104459954702 30.700912007080856 17.547531101668685 13 22.178432898204044 31.346085427266495 28.048628807622332 18.426852866907133 14 18.026674688549438 35.18082500050619 28.406576401202127 18.38592390974225 15 17.97186170351237 35.96946670870442 28.081603373818776 17.97706821396444 16 20.12171664434616 35.23303473031722 26.871523569583566 17.77372505575305 17 19.82682567735255 34.60839810135919 27.258540846520752 18.306235374767514 18 20.329109310686942 33.49001073119655 26.963071378365793 19.217808579750724 19 19.267270429045386 36.941059409176766 26.84028450687115 16.9513856549067 20 21.20640632186069 35.66141484029029 25.289756769186717 17.8424220686623 21 21.102276112819297 34.753312642275134 26.803838933706658 17.340572311198915 22 19.37935502905522 33.4943494899066 27.387112729628804 19.739182751409373 23 17.97489883460941 35.69699266171277 26.689006453180454 19.639102050497367 24 17.418525342689627 35.308384506581895 27.844562522959265 19.428527627769213 25 18.97845372424585 36.79701262000283 25.697600087932177 18.526933567819135 26 19.87686602780855 35.50941366014792 24.802080290176182 19.811640021867344 27 17.57067114812233 35.13859441572829 26.923299423523595 20.367435012625787 28 18.996676510828095 35.61600249912502 27.198087475160605 18.18923351488628 29 19.83289993954663 34.572820279936714 26.73629892312009 18.857980857396573 30 20.577575559482934 33.703043783860394 26.68379994272839 19.035580713928283 31 21.03589310455541 35.2190060771547 24.088643732952296 19.656457085337596 32 20.22483447635521 33.356521588217085 26.87311444777725 19.545529487650445 33 19.384127663636285 34.32189540120502 26.23661854501173 20.057358390146966 34 18.569164152597036 34.571229401743025 28.000613211231023 18.858993234428915 35 19.396999314476123 33.09417131154891 29.05261757312978 18.45621180084519 36 19.790524729478395 34.131713144414135 27.373517952337288 18.704244173770178 37 18.84120432371768 34.474908958379736 26.90522126223168 19.7786654556709 38 19.50807153745361 34.35168821101408 27.063152079277796 19.077088172254506 39 20.306981641265644 31.242099843515437 26.7970415450609 21.653876970158016 40 21.034157601071385 32.36149959071043 26.729067658603324 19.87527514961486 41 20.37408777598121 31.176150711122553 28.16707692040692 20.282684592489318 42 20.5749723042569 32.43366761058773 26.21521400204211 20.77614608311326 43 19.33958307421302 32.034935685133384 27.979353293551736 20.646127947101853 44 20.546191871480183 30.769753645280446 27.466945889893875 21.2171085933455 45 21.130044168563668 31.232554574353312 27.436429953633134 20.20097130344989 46 19.139132421808334 31.734838207687705 28.198171357829 20.92785801267496 47 19.602511852042543 32.508728136271735 27.658429774297772 20.230330237387953 48 20.549662878448228 31.108610700535984 28.130486721952092 20.211239699063697 49 20.192004535449104 30.631925743590934 28.34366439990628 20.83240532105368 50 18.774387439582785 31.125676484795544 28.21408013976588 21.88585593585579 51 18.643212301248695 30.38996763286002 28.81167183943122 22.155148226460064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29385.0 1 21728.0 2 14071.0 3 8839.5 4 3608.0 5 3010.5 6 2413.0 7 2510.5 8 2608.0 9 3259.5 10 3911.0 11 3903.0 12 3895.0 13 4052.5 14 4210.0 15 4199.0 16 4188.0 17 4650.5 18 5113.0 19 4750.5 20 4388.0 21 4520.5 22 4653.0 23 5361.0 24 6069.0 25 6608.0 26 9094.0 27 11041.0 28 12289.0 29 13537.0 30 15793.0 31 18049.0 32 19924.0 33 21799.0 34 23203.0 35 24607.0 36 24394.0 37 24181.0 38 26950.0 39 29719.0 40 29832.0 41 29945.0 42 34392.0 43 38839.0 44 40761.5 45 42684.0 46 45615.5 47 48547.0 48 50260.0 49 51973.0 50 51246.5 51 50520.0 52 46032.0 53 41544.0 54 37327.0 55 33110.0 56 29879.5 57 26649.0 58 24125.0 59 21601.0 60 19467.0 61 17333.0 62 16194.0 63 15055.0 64 12682.5 65 10310.0 66 9060.0 67 7810.0 68 6728.0 69 5646.0 70 4883.0 71 4120.0 72 3466.0 73 2812.0 74 2477.5 75 1534.0 76 925.0 77 711.0 78 497.0 79 415.0 80 333.0 81 302.0 82 271.0 83 171.0 84 71.0 85 71.5 86 72.0 87 47.5 88 23.0 89 16.5 90 10.0 91 5.5 92 1.0 93 2.5 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 691442.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.71633591313663 #Duplication Level Percentage of deduplicated Percentage of total 1 77.68462724120975 19.200793421805447 2 9.238827411447451 4.566999234896595 3 3.3767908038693077 2.503856874504734 4 1.7201977173481708 1.7006793847595298 5 1.0604870535461095 1.3105677123489075 6 0.7532030235479229 1.116985136448037 7 0.5480403800551727 0.9481885089184698 8 0.43060190548772925 0.851432107269714 9 0.3725005921733491 0.8286164787598927 >10 3.2693728593572486 17.98443807552277 >50 0.8913216302735947 15.70272148893369 >100 0.6463557517546928 26.67901572379792 >500 0.004131954577427261 0.5933565761915203 >1k 0.002361116901387006 1.493531170512071 >5k 5.902792253467515E-4 0.8006739341379551 >10k+ 5.902792253467515E-4 3.718144171192745 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25485 3.6857755241943653 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5488 0.7937035933599637 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4511 0.6524046847024045 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2917 0.4218719719079836 No Hit CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT 1743 0.25208188105437623 No Hit GACAATGGGGTGAAATCTAACTGTACTGAGGTCCTTTGTTTTTTATATGTA 1066 0.1541705594973982 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.892505806705407E-4 0.0 0.0 0.31325837886619556 0.0 2 2.892505806705407E-4 0.0 0.0 0.45484653810442527 0.0 3 2.892505806705407E-4 0.0 0.0 0.6065584676661239 0.0 4 2.892505806705407E-4 0.0 0.0 1.3461722024406964 0.0 5 2.892505806705407E-4 0.0 0.0 1.4832769776785326 0.0 6 2.892505806705407E-4 0.0 0.0 2.218985829614053 0.0 7 2.892505806705407E-4 0.0 0.0 2.7862062183089833 0.0 8 2.892505806705407E-4 0.0 0.0 3.2539244072532476 0.0 9 2.892505806705407E-4 0.0 0.0 3.9018457079552586 0.0 10 2.892505806705407E-4 0.0 0.0 4.987547762502134 0.0 11 2.892505806705407E-4 0.0 0.0 5.893191330581596 0.0 12 2.892505806705407E-4 0.0 0.0 6.360041767783849 0.0 13 2.892505806705407E-4 0.0 0.0 6.5509471510264055 0.0 14 2.892505806705407E-4 0.0 0.0 6.752699431044108 0.0 15 2.892505806705407E-4 0.0 0.0 6.924658901252744 0.0 16 2.892505806705407E-4 0.0 0.0 7.285788251219914 0.0 17 2.892505806705407E-4 0.0 0.0 7.755386568938537 0.0 18 2.892505806705407E-4 0.0 0.0 8.334900107311965 0.0 19 2.892505806705407E-4 0.0 0.0 8.601155266819198 0.0 20 2.892505806705407E-4 0.0 0.0 8.888814969296051 0.0 21 2.892505806705407E-4 0.0 0.0 9.171846662482174 0.0 22 2.892505806705407E-4 0.0 0.0 9.478741528573618 0.0 23 2.892505806705407E-4 0.0 0.0 9.779706757761316 0.0 24 2.892505806705407E-4 0.0 0.0 10.012987351072107 0.0 25 2.892505806705407E-4 0.0 0.0 10.19969860089494 0.0 26 2.892505806705407E-4 0.0 0.0 10.389880857685823 0.0 27 2.892505806705407E-4 0.0 0.0 10.60855429667275 0.0 28 2.892505806705407E-4 0.0 0.0 10.827661611530685 0.0 29 2.892505806705407E-4 0.0 0.0 11.067739593487234 0.0 30 2.892505806705407E-4 0.0 0.0 11.367403195061915 0.0 31 2.892505806705407E-4 0.0 0.0 11.589692266307225 0.0 32 2.892505806705407E-4 0.0 0.0 11.823985236650362 0.0 33 2.892505806705407E-4 0.0 0.0 12.05307169654143 0.0 34 2.892505806705407E-4 0.0 0.0 12.265670873334278 0.0 35 2.892505806705407E-4 0.0 0.0 12.518620506130667 0.0 36 2.892505806705407E-4 0.0 0.0 12.731798184084855 0.0 37 2.892505806705407E-4 0.0 0.0 12.969996037267045 0.0 38 2.892505806705407E-4 0.0 0.0 13.204867508771525 0.0 39 2.892505806705407E-4 0.0 0.0 13.469387164794734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGT 20 7.0313347E-4 45.0 25 CTTCGCA 25 3.8891878E-5 45.0 15 CGTGGTA 20 7.0313347E-4 45.0 15 AGCGTCA 25 3.8891878E-5 45.0 1 ACTATCG 25 3.8891878E-5 45.0 1 CGGCGCA 35 1.2109922E-7 45.0 26 TCGCATC 25 3.8891878E-5 45.0 29 CCTTACG 35 1.2109922E-7 45.0 32 TCGCACT 25 3.8891878E-5 45.0 17 ACACGGA 25 3.8891878E-5 45.0 28 ACCGGAC 25 3.8891878E-5 45.0 14 CAACGTA 20 7.0313347E-4 45.0 22 CTCCGAT 35 1.2109922E-7 45.0 43 CGACGTG 35 1.2109922E-7 45.0 15 CGACGTC 45 3.8380676E-10 45.0 43 CGACGTA 45 3.8380676E-10 45.0 23 TACCACG 25 3.8891878E-5 45.0 1 GAATGCG 25 3.8891878E-5 45.0 40 ACACCCG 25 3.8891878E-5 45.0 1 GACGTAA 20 7.0313347E-4 45.0 22 >>END_MODULE