##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684919
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57522568362098	31.0	31.0	34.0	30.0	34.0
2	31.78149679013139	31.0	31.0	34.0	30.0	34.0
3	31.96888099176691	33.0	31.0	34.0	30.0	34.0
4	35.70784282520999	37.0	35.0	37.0	33.0	37.0
5	35.465719304034494	37.0	35.0	37.0	33.0	37.0
6	32.66115555270039	37.0	35.0	37.0	26.0	37.0
7	34.21120745664816	37.0	35.0	37.0	27.0	37.0
8	35.29864991334742	37.0	35.0	37.0	32.0	37.0
9	37.142064974106425	39.0	37.0	39.0	34.0	39.0
10	36.61789788281534	38.0	35.0	39.0	32.0	39.0
11	36.6774260898004	39.0	35.0	39.0	32.0	39.0
12	36.72217590693206	39.0	35.0	39.0	32.0	39.0
13	36.738712168884206	39.0	37.0	39.0	32.0	39.0
14	37.865695067591936	40.0	37.0	41.0	33.0	41.0
15	37.90728392700451	40.0	37.0	41.0	33.0	41.0
16	37.784475244517964	40.0	37.0	41.0	33.0	41.0
17	37.726623148138685	39.0	37.0	41.0	33.0	41.0
18	37.50058620070403	39.0	36.0	41.0	32.0	41.0
19	37.40477487118915	39.0	35.0	41.0	32.0	41.0
20	37.28115733393292	39.0	35.0	40.0	32.0	41.0
21	37.2327516100444	39.0	35.0	40.0	32.0	41.0
22	37.15678058281344	39.0	35.0	40.0	32.0	41.0
23	36.950427714810075	39.0	35.0	40.0	31.0	41.0
24	36.881226831201936	39.0	35.0	40.0	31.0	41.0
25	36.875773631626515	39.0	35.0	40.0	31.0	41.0
26	36.79721689718054	39.0	35.0	40.0	31.0	41.0
27	36.7211465881367	39.0	35.0	40.0	31.0	41.0
28	36.660881067688294	39.0	35.0	40.0	31.0	41.0
29	36.455472836934	38.0	35.0	40.0	30.0	41.0
30	36.440393681588624	38.0	35.0	40.0	30.0	41.0
31	36.008830241240204	38.0	35.0	40.0	29.0	41.0
32	36.13291060694768	38.0	35.0	40.0	30.0	41.0
33	36.07753909586389	38.0	35.0	40.0	30.0	41.0
34	35.99271738701949	38.0	35.0	40.0	30.0	41.0
35	35.95232428944153	38.0	35.0	40.0	30.0	41.0
36	35.86259397096591	38.0	35.0	40.0	29.0	41.0
37	35.78723323487887	38.0	35.0	40.0	29.0	41.0
38	35.69802852600088	38.0	35.0	40.0	28.0	41.0
39	35.61208259662821	38.0	35.0	40.0	27.0	41.0
40	35.45883381830552	38.0	34.0	40.0	27.0	41.0
41	35.3982616922585	38.0	34.0	40.0	27.0	41.0
42	35.23870997884421	38.0	34.0	40.0	26.0	41.0
43	35.175086397077614	38.0	34.0	40.0	26.0	41.0
44	34.98965279105997	38.0	34.0	40.0	25.0	41.0
45	34.768146306351554	38.0	34.0	40.0	24.0	41.0
46	34.51362277875194	37.0	33.0	40.0	23.0	41.0
47	34.484950775201156	37.0	33.0	40.0	23.0	41.0
48	34.47099876043737	37.0	33.0	40.0	23.0	41.0
49	34.32711751316579	37.0	33.0	40.0	23.0	41.0
50	34.162489287054385	37.0	33.0	40.0	23.0	41.0
51	33.14614866867469	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	4.0
11	17.0
12	17.0
13	21.0
14	22.0
15	60.0
16	131.0
17	293.0
18	571.0
19	1088.0
20	1788.0
21	2470.0
22	3687.0
23	4599.0
24	5738.0
25	6433.0
26	7307.0
27	8308.0
28	10084.0
29	12232.0
30	15032.0
31	19595.0
32	25027.0
33	32917.0
34	47621.0
35	69590.0
36	68440.0
37	91730.0
38	125101.0
39	124972.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.3454437678032	34.75374460337646	18.33048871472393	25.570322914096412
2	21.991359562225608	34.86748068019722	18.196604269993973	24.9445554875832
3	19.729194255087098	36.85078089525915	18.07892612119097	25.34109872846278
4	18.642350409318475	37.17169475514623	18.28595790159128	25.899996933944013
5	17.6639865443943	39.39473134779441	15.74536551037422	27.19591659743707
6	16.766362153772928	45.97010741416138	16.081463647526203	21.182066784539487
7	80.17488199334521	12.790563555690525	3.881334873174784	3.153219577789491
8	81.41517464108895	12.377084005553941	3.185194161645392	3.0225471917117206
9	74.79088768160906	16.194031703018897	5.387644378386349	3.6274362369856874
10	29.463922011215928	46.989498028233996	11.718173973856763	11.828405986693317
11	19.076562338028292	33.721213749363066	30.951105167180355	16.251118745428293
12	21.42735126343407	30.4389278148219	30.681730248394334	17.451990673349695
13	22.1728408760744	31.494381087398654	27.908555610225445	18.424222426301505
14	17.96635806569828	35.0723224206074	28.505706514200952	18.455612999493372
15	17.93525949783843	36.129235719844246	28.057916337552324	17.877588444765003
16	20.209835031587676	35.147951801599895	26.933549806619467	17.708663360192958
17	19.82643203064888	34.61577208399825	27.331407071493125	18.226388813859742
18	20.266192060667027	33.61579982450479	26.980562665074263	19.137445449753912
19	19.348127296804442	36.95298276146522	26.852226321652633	16.846663620077702
20	21.261930242846233	35.72597635632827	25.38986361890968	17.62222978191582
21	21.13373990209061	34.8184237844183	26.75834660740905	17.289489706082033
22	19.342871200828128	33.60032354190787	27.321186884872517	19.73561837239148
23	18.006362796184657	35.92468598476608	26.541970656384184	19.526980562665074
24	17.366287108402602	35.40082841912693	27.948122332713794	19.284762139756673
25	18.926763602703385	36.72448858916164	25.79458300908575	18.55416479904923
26	19.72452216977482	35.63165863408666	24.812277072179338	19.831542123959185
27	17.556382579545904	35.24489757182966	26.994578921011097	20.204140927613338
28	19.049551844816687	35.57982768765358	27.163212000251125	18.20740846727861
29	19.911551584931942	34.6601568944649	26.584749437524728	18.84354208307844
30	20.506804454249334	33.796405122357534	26.72841606087727	18.96837436251586
31	21.232583706978488	35.17934237479176	24.00167027049914	19.586403647730606
32	20.220931234204336	33.42161627871325	26.89938518277344	19.458067304308976
33	19.52712656533108	34.477507559288036	26.137835276872156	19.85753059850873
34	18.60847779080446	34.71884996620038	27.920089820840126	18.75258242215503
35	19.428282760443206	33.22991477824385	29.07818296762099	18.263619493691955
36	19.800297553433328	34.20871665116605	27.38367602592423	18.60730976947639
37	18.762802608775637	34.529338505721114	26.95326016653064	19.75459871897261
38	19.51092026940412	34.43458277548148	27.046409867444183	19.00808708767022
39	20.27904029527579	31.22690420327075	26.794409265913195	21.69964623554026
40	21.08439100097968	32.43726630448272	26.581391376206533	19.896951318331073
41	20.302692727169198	31.31246176555184	28.127413606572453	20.257431900706507
42	20.522426739512266	32.63290987693435	26.169809860728055	20.674853522825327
43	19.439524965725873	32.09489005269236	27.873076962385333	20.59250801919643
44	20.573089664617275	30.8207247864346	27.420030689760395	21.186154859187727
45	21.20893127508508	31.208069859355632	27.47799374816584	20.105005117393443
46	19.279944051778386	31.795584587374563	28.090912940070282	20.83355842077676
47	19.626262375550976	32.55728049594185	27.662687120666824	20.153770007840343
48	20.534544960790985	31.224130152616585	28.08624085475801	20.155084031834424
49	20.122817442646504	30.774149935977828	28.308456912423217	20.794575708952443
50	18.709949643680492	31.316987848198107	28.206985059547186	21.76607744857421
51	18.548908703072918	30.4793705533063	29.057450589047757	21.914270154573025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29232.0
1	21666.0
2	14100.0
3	8809.0
4	3518.0
5	2936.0
6	2354.0
7	2500.0
8	2646.0
9	3247.0
10	3848.0
11	3863.0
12	3878.0
13	4034.5
14	4191.0
15	4212.5
16	4234.0
17	4662.0
18	5090.0
19	4697.5
20	4305.0
21	4470.5
22	4636.0
23	5402.5
24	6169.0
25	6685.0
26	9203.0
27	11205.0
28	12530.5
29	13856.0
30	16145.5
31	18435.0
32	20144.5
33	21854.0
34	23058.5
35	24263.0
36	24071.0
37	23879.0
38	26575.0
39	29271.0
40	29595.5
41	29920.0
42	34212.0
43	38504.0
44	40420.0
45	42336.0
46	45188.5
47	48041.0
48	49458.0
49	50875.0
50	50154.5
51	49434.0
52	45201.0
53	40968.0
54	36944.0
55	32920.0
56	29361.0
57	25802.0
58	23639.5
59	21477.0
60	19251.5
61	17026.0
62	15902.0
63	14778.0
64	12530.0
65	10282.0
66	8970.0
67	7658.0
68	6581.5
69	5505.0
70	4867.5
71	4230.0
72	3477.5
73	2725.0
74	2443.5
75	1508.5
76	855.0
77	686.0
78	517.0
79	421.0
80	325.0
81	296.5
82	268.0
83	160.5
84	53.0
85	54.5
86	56.0
87	37.5
88	19.0
89	12.5
90	6.0
91	4.0
92	2.0
93	4.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.012463377394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42747236337864	19.366518168931943
2	9.08782990025063	4.546180251200102
3	3.4082401154123616	2.557454432043504
4	1.7743958892425669	1.7752804878671264
5	1.092798128684706	1.3666786586305455
6	0.7668452055045973	1.1508412571288353
7	0.6019362443928252	1.0539135787881138
8	0.45884196942056865	0.918141436491465
9	0.3894656587570283	0.876734597377152
>10	3.50605045649127	19.155946633660953
>50	0.8719695804648646	15.554974355913423
>100	0.6064849314735322	25.103905592417753
>500	0.004129761206531834	0.5629604800998919
>1k	0.0023598635465896197	1.4700425433172013
>5k	5.899658866474049E-4	0.7995071772427822
>10k+	5.899658866474049E-4	3.7409203488892167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25351	3.7013135859860804	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5418	0.7910424444350355	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4428	0.6464998050864409	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2855	0.41683761145478515	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	1661	0.24251042824041968	No Hit
GACAATGGGGTGAAATCTAACTGTACTGAGGTCCTTTGTTTTTTATATGTA	1018	0.14863071399683758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.460026660086813E-4	0.0	0.0	0.2940493693414842	0.0
2	1.460026660086813E-4	0.0	0.0	0.4304158593935925	0.0
3	1.460026660086813E-4	0.0	0.0	0.5724764534200395	0.0
4	1.460026660086813E-4	0.0	0.0	1.2718292236016229	0.0
5	1.460026660086813E-4	0.0	0.0	1.4096557403138181	0.0
6	1.460026660086813E-4	0.0	0.0	2.1126585771456186	0.0
7	1.460026660086813E-4	0.0	0.0	2.6845510199016234	0.0
8	1.460026660086813E-4	0.0	0.0	3.115112881961225	0.0
9	1.460026660086813E-4	0.0	0.0	3.767306791022004	0.0
10	1.460026660086813E-4	0.0	0.0	4.847142508822211	0.0
11	1.460026660086813E-4	0.0	0.0	5.734546712822977	0.0
12	1.460026660086813E-4	0.0	0.0	6.189637022772036	0.0
13	1.460026660086813E-4	0.0	0.0	6.377834459257226	0.0
14	1.460026660086813E-4	0.0	0.0	6.5799021490132406	0.0
15	1.460026660086813E-4	0.0	0.0	6.747951217589233	0.0
16	1.460026660086813E-4	0.0	0.0	7.096605584017964	0.0
17	1.460026660086813E-4	0.0	0.0	7.551257885968997	0.0
18	1.460026660086813E-4	0.0	0.0	8.111616118110316	0.0
19	1.460026660086813E-4	0.0	0.0	8.364346732971343	0.0
20	1.460026660086813E-4	0.0	0.0	8.641897801053847	0.0
21	1.460026660086813E-4	0.0	0.0	8.905724618531535	0.0
22	1.460026660086813E-4	0.0	0.0	9.202256033195166	0.0
23	1.460026660086813E-4	0.0	0.0	9.479223090613635	0.0
24	1.460026660086813E-4	0.0	0.0	9.697789081628631	0.0
25	1.460026660086813E-4	0.0	0.0	9.872554272841022	0.0
26	1.460026660086813E-4	0.0	0.0	10.051991549365692	0.0
27	1.460026660086813E-4	0.0	0.0	10.27041153771468	0.0
28	1.460026660086813E-4	0.0	0.0	10.478027328779023	0.0
29	1.460026660086813E-4	0.0	0.0	10.707397517078661	0.0
30	1.460026660086813E-4	0.0	0.0	10.990350683803486	0.0
31	1.460026660086813E-4	0.0	0.0	11.197236461537788	0.0
32	1.460026660086813E-4	0.0	0.0	11.420766543197079	0.0
33	1.460026660086813E-4	0.0	0.0	11.65305678481689	0.0
34	1.460026660086813E-4	0.0	0.0	11.865344661193513	0.0
35	1.460026660086813E-4	0.0	0.0	12.113257188076254	0.0
36	1.460026660086813E-4	0.0	0.0	12.311674811182051	0.0
37	1.460026660086813E-4	0.0	0.0	12.543089036805812	0.0
38	1.460026660086813E-4	0.0	0.0	12.774357259763564	0.0
39	1.460026660086813E-4	0.0	0.0	13.02927791461472	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGG	60	0.0	45.000004	2
GATACGA	25	3.8891485E-5	45.000004	8
CCTTACG	25	3.8891485E-5	45.000004	32
CGAACTT	25	3.8891485E-5	45.000004	24
CGAACAC	25	3.8891485E-5	45.000004	12
TCCGATC	25	3.8891485E-5	45.000004	15
ATTACTC	30	2.1642118E-6	45.000004	29
ATTACGA	25	3.8891485E-5	45.000004	40
TTACTCG	30	2.1642118E-6	45.000004	30
TACGGTG	30	2.1642118E-6	45.000004	28
TACGGCA	25	3.8891485E-5	45.000004	35
CGACAAT	25	3.8891485E-5	45.000004	19
TCTAGCG	25	3.8891485E-5	45.000004	1
AATGCGT	30	2.1642118E-6	45.000004	41
AATGCGC	50	2.1827873E-11	45.000004	14
CCCGTAA	25	3.8891485E-5	45.000004	19
TACGCGG	50	2.1827873E-11	45.000004	2
CCGTAAC	25	3.8891485E-5	45.000004	20
CGTAGCG	100	0.0	45.000004	1
TACCTAC	30	2.1642118E-6	45.000004	20
>>END_MODULE