##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 744014 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.723001180085323 31.0 31.0 34.0 30.0 34.0 2 31.92438851957087 33.0 31.0 34.0 30.0 34.0 3 32.09037329942716 34.0 31.0 34.0 30.0 34.0 4 35.79898227721522 37.0 35.0 37.0 35.0 37.0 5 35.541173956404045 37.0 35.0 37.0 33.0 37.0 6 34.17356393831299 37.0 35.0 37.0 32.0 37.0 7 34.96838097132581 37.0 35.0 37.0 32.0 37.0 8 35.681425349522996 37.0 35.0 37.0 33.0 37.0 9 37.397073173354265 39.0 37.0 39.0 35.0 39.0 10 36.80911111887679 39.0 37.0 39.0 32.0 39.0 11 36.77208358982492 39.0 37.0 39.0 32.0 39.0 12 36.71323926700304 39.0 35.0 39.0 32.0 39.0 13 36.701226052197946 39.0 35.0 39.0 32.0 39.0 14 37.88468496560549 40.0 37.0 41.0 33.0 41.0 15 37.88388256134965 40.0 37.0 41.0 33.0 41.0 16 37.80476442647585 40.0 37.0 41.0 33.0 41.0 17 37.76352192297457 39.0 37.0 41.0 33.0 41.0 18 37.65933571142479 39.0 36.0 41.0 33.0 41.0 19 37.64964906574339 39.0 36.0 41.0 33.0 41.0 20 37.524902757206185 39.0 36.0 41.0 32.0 41.0 21 37.47947888077375 39.0 36.0 41.0 32.0 41.0 22 37.47090242925536 39.0 36.0 41.0 32.0 41.0 23 37.311120489668205 39.0 35.0 41.0 32.0 41.0 24 37.10982857849449 39.0 35.0 41.0 31.0 41.0 25 37.18867655716156 39.0 35.0 41.0 32.0 41.0 26 37.12882150067069 39.0 35.0 41.0 32.0 41.0 27 37.056705922200386 39.0 35.0 41.0 31.0 41.0 28 36.97007314378493 39.0 35.0 41.0 31.0 41.0 29 36.82915375248315 39.0 35.0 40.0 31.0 41.0 30 36.809049292083216 39.0 35.0 40.0 31.0 41.0 31 36.74494565962468 39.0 35.0 40.0 31.0 41.0 32 36.65045415812068 39.0 35.0 40.0 31.0 41.0 33 36.606350149325145 39.0 35.0 40.0 30.0 41.0 34 36.5072659923066 39.0 35.0 40.0 30.0 41.0 35 36.404232984863185 39.0 35.0 40.0 30.0 41.0 36 36.35023131285164 39.0 35.0 40.0 30.0 41.0 37 36.25886206442352 38.0 35.0 40.0 30.0 41.0 38 36.18731099145984 38.0 35.0 40.0 30.0 41.0 39 36.11782842795969 38.0 35.0 40.0 30.0 41.0 40 35.99135365732365 38.0 35.0 40.0 29.0 41.0 41 35.94253468348714 38.0 35.0 40.0 29.0 41.0 42 35.84389675463096 38.0 35.0 40.0 29.0 41.0 43 35.76254479082383 38.0 35.0 40.0 29.0 41.0 44 35.63733881351695 38.0 35.0 40.0 28.0 41.0 45 35.485453230718775 38.0 34.0 40.0 28.0 41.0 46 35.29718257989769 38.0 34.0 40.0 27.0 41.0 47 35.21053367275347 38.0 34.0 40.0 27.0 41.0 48 35.127308088288665 37.0 34.0 40.0 27.0 41.0 49 34.95419978656316 37.0 34.0 40.0 26.0 41.0 50 34.86982906235635 37.0 34.0 40.0 26.0 41.0 51 33.640881757601335 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 5.0 10 10.0 11 13.0 12 24.0 13 14.0 14 34.0 15 56.0 16 94.0 17 214.0 18 414.0 19 752.0 20 1223.0 21 1877.0 22 2754.0 23 3411.0 24 4342.0 25 5135.0 26 6358.0 27 7636.0 28 9200.0 29 11514.0 30 15069.0 31 19360.0 32 25389.0 33 34516.0 34 51282.0 35 67521.0 36 73390.0 37 101291.0 38 145039.0 39 156048.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.74342687099974 32.60812296542807 19.418317397253276 27.230132766318917 2 22.658444599160767 32.113105398554325 19.31361506638316 25.914834935901744 3 20.534694239624525 31.972919864411153 20.998529597561337 26.493856298402985 4 20.013467488515 33.25690107981839 19.472751856819897 27.25687957484671 5 17.51781552497668 37.11072641106215 17.084087127392763 28.287370936568397 6 18.105035657931168 42.23253863502568 17.555986849709818 22.10643885733333 7 83.41670990062015 9.135580782082057 4.1249223804928405 3.322786936804953 8 85.29718526801915 7.354297096560011 3.616464206318698 3.7320534291021406 9 78.65967575878948 10.81296857317201 6.281199009696054 4.24615665834245 10 36.03158005091302 36.39676135126489 12.706750141798405 14.864908456023675 11 25.03501278201754 31.28744889209074 24.71431989182999 18.963218434061723 12 25.191192638848193 26.533774902085177 29.842852419443723 18.43218003962291 13 24.385025012970186 26.366036122976183 27.29142193560874 21.957516928444896 14 20.045187321743946 29.872959379796615 28.820156609956264 21.26169668850317 15 19.601781686903742 31.236508990422223 28.151217584615345 21.010491738058693 16 21.88440002473072 29.79150929955619 26.93443940571011 21.389651270002986 17 20.987239487429 29.313023679661942 28.215194875365246 21.484541957543808 18 22.076331896980435 29.785326620198006 26.29654818323311 21.84179329958845 19 21.367474267957324 30.877913587647544 26.636864360079244 21.11774778431589 20 23.14916117169838 30.363541546261224 26.22464093417597 20.26265634786442 21 22.708712470464263 30.10507866787453 26.662670326096016 20.52353853556519 22 22.489764977540748 29.49124613246525 25.957038442825 22.061950447169004 23 21.57459402645649 30.353595496858933 26.10622918385944 21.965581292825135 24 18.86940299510493 31.41661312824759 28.350407384807276 21.36357649184021 25 20.628106460362304 31.420914122583714 24.744830070401903 23.20614934665208 26 22.349041819105555 30.75788896445497 23.732080310316743 23.160988906122736 27 20.161582980965413 30.632891316561246 26.66119185929297 22.544333843180368 28 20.74248602848871 30.472276059321462 28.35027297873427 20.434964933455554 29 22.238291214950255 29.947285938167827 25.54185808331564 22.272564763566276 30 20.759690005833225 30.339751671339517 27.772998895182077 21.127559427645178 31 22.39863766004403 30.81958135196381 23.44969315093533 23.332087837056832 32 22.03453160827619 30.528054579618125 25.925856233888073 21.511557578217612 33 22.73707215186811 30.113277438327774 25.050335074339998 22.099315335464116 34 19.730542704841575 29.081710828022057 27.510638240678265 23.677108226458106 35 20.687782756776084 28.17406661702602 28.2102218506641 22.927928775533793 36 22.162217377629993 30.268650858720402 26.726244398626907 20.8428873650227 37 21.253766730195935 30.293515982226143 26.20353918071434 22.24917810686358 38 21.20040751921335 30.659369312943035 26.671541126914278 21.468682040929338 39 21.696231522525114 28.76249640463755 25.9839196574258 23.557352415411536 40 21.385619087812863 28.832118750453624 27.032018214711012 22.750243947022504 41 20.26494125110549 27.985360490528404 28.319090769797345 23.430607488568764 42 20.924337445263127 29.31221724322392 26.337273223353325 23.426172088159632 43 21.082802205334847 28.760749125688495 26.234990201797277 23.92145846717938 44 22.004693460069298 26.56979572965025 27.53362167916195 23.8918891311185 45 22.71287905872739 27.765606561166862 28.362503931377635 21.159010448728115 46 20.15540030160723 28.32863360098063 27.857137096882585 23.65882900052956 47 21.79582642262108 28.908461399919894 26.86226334450696 22.43344883295207 48 20.80162470061047 27.80633160128707 28.587230885440327 22.804812812662128 49 20.547328410486898 29.249718419277055 27.98697336340445 22.215979806831594 50 20.993422166787184 27.33980812189018 28.37984231479515 23.286927396527485 51 21.386156712104878 26.58269871265863 28.62096680976433 23.410177765472152 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15849.0 1 11810.5 2 7772.0 3 4851.0 4 1930.0 5 1606.5 6 1283.0 7 1303.5 8 1324.0 9 1460.5 10 1597.0 11 1822.5 12 2048.0 13 1891.0 14 1734.0 15 1949.5 16 2165.0 17 2306.0 18 2447.0 19 2603.0 20 2759.0 21 2992.0 22 3225.0 23 5837.0 24 8449.0 25 8226.5 26 9481.5 27 10959.0 28 11527.0 29 12095.0 30 13262.5 31 14430.0 32 16625.0 33 18820.0 34 22540.0 35 26260.0 36 26078.5 37 25897.0 38 27828.5 39 29760.0 40 30418.0 41 31076.0 42 33560.5 43 36045.0 44 40304.5 45 44564.0 46 48632.0 47 52700.0 48 53203.0 49 53706.0 50 53078.0 51 52450.0 52 50269.0 53 48088.0 54 42905.0 55 37722.0 56 36506.0 57 35290.0 58 33145.0 59 31000.0 60 29736.5 61 28473.0 62 26031.5 63 23590.0 64 21933.0 65 20276.0 66 17498.5 67 14721.0 68 12657.0 69 10593.0 70 9377.0 71 8161.0 72 7228.5 73 6296.0 74 5415.0 75 3500.5 76 2467.0 77 2126.0 78 1785.0 79 1339.0 80 893.0 81 671.5 82 450.0 83 295.5 84 141.0 85 105.5 86 70.0 87 74.5 88 79.0 89 46.5 90 14.0 91 11.5 92 9.0 93 8.0 94 7.0 95 6.0 96 5.0 97 3.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 744014.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.65568212268251 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14505176835291 16.70628718433337 2 9.209596729341186 3.9888019849741845 3 3.4527336067142755 2.2431390432392235 4 1.7943209557721278 1.5542897697707625 5 1.0727535121009852 1.161560452702509 6 0.742139621918864 0.9642923845753619 7 0.5234256263327263 0.7934597285109259 8 0.4174552734999112 0.7232222962681251 9 0.3016518073780666 0.5879228087080859 >10 3.222260423984707 16.367628500768095 >50 1.1755438111675536 18.433131951732992 >100 0.9336675259273213 32.43920743330788 >500 0.005012980004978906 0.7477035664103475 >1k 0.00375973500373418 1.4173799911354046 >5k 0.0 0.0 >10k+ 6.266225006223633E-4 1.8719729035627484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13795 1.854131777090216 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2909 0.39098726636864356 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2461 0.33077334566285044 No Hit CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT 1699 0.22835591803380043 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1342 0.18037294997137152 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT 1018 0.13682538231807467 No Hit AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 1016 0.13655657017206665 No Hit AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT 864 0.11612684707545824 No Hit CCGGGAAGGGCTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATT 847 0.11384194383439021 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC 776 0.10429911265110603 No Hit ACTTCAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGC 754 0.10134217904501797 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.2751292314391933 0.0 2 0.0 0.0 0.0 0.37781547121425135 0.0 3 0.0 0.0 0.0 0.515716102116358 0.0 4 0.0 0.0 0.0 1.1714833323028868 0.0 5 0.0 0.0 0.0 1.2736319477859288 0.0 6 0.0 0.0 0.0 1.855475837820256 0.0 7 0.0 0.0 0.0 2.27737650097982 0.0 8 0.0 0.0 0.0 2.6121820288327906 0.0 9 0.0 0.0 0.0 3.242949729440575 0.0 10 0.0 0.0 0.0 4.070756733072227 0.0 11 0.0 0.0 0.0 4.833914415588954 0.0 12 0.0 0.0 0.0 5.168182319149908 0.0 13 0.0 0.0 0.0 5.3113247868991715 0.0 14 0.0 0.0 0.0 5.473284104868994 0.0 15 0.0 0.0 0.0 5.617098602983277 0.0 16 0.0 0.0 0.0 5.90230828989777 0.0 17 0.0 0.0 0.0 6.252032891854186 0.0 18 0.0 0.0 0.0 6.749200955895991 0.0 19 0.0 0.0 0.0 6.948390756087923 0.0 20 0.0 0.0 0.0 7.195966742561296 0.0 21 0.0 0.0 0.0 7.439779358990557 0.0 22 0.0 0.0 0.0 7.689102624412982 0.0 23 0.0 0.0 0.0 7.938694701981414 0.0 24 0.0 0.0 0.0 8.144470399750542 0.0 25 0.0 0.0 0.0 8.333042120175158 0.0 26 0.0 0.0 0.0 8.497151935313045 0.0 27 0.0 0.0 0.0 8.681960285693549 0.0 28 0.0 0.0 0.0 8.86770947858508 0.0 29 0.0 0.0 0.0 9.055609168644676 0.0 30 0.0 0.0 0.0 9.290954202474685 0.0 31 0.0 0.0 0.0 9.490816033031637 0.0 32 0.0 0.0 0.0 9.688392960347521 0.0 33 0.0 0.0 0.0 9.894168658116648 0.0 34 0.0 0.0 0.0 10.08999830648348 0.0 35 0.0 0.0 0.0 10.315800509130204 0.0 36 0.0 0.0 0.0 10.5272212619655 0.0 37 0.0 0.0 0.0 10.725739031792413 0.0 38 0.0 0.0 0.0 10.939847906087788 0.0 39 0.0 0.0 0.0 11.152209501434113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGAGT 35 1.2111559E-7 45.000004 14 CTATCGA 35 1.2111559E-7 45.000004 17 GCGCCTA 35 1.2111559E-7 45.000004 9 CACGATT 35 1.2111559E-7 45.000004 36 ACCGTTG 35 1.2111559E-7 45.000004 34 TCGACCG 35 1.2111559E-7 45.000004 24 TATCGAT 35 1.2111559E-7 45.000004 18 TATTTCG 20 7.0316816E-4 45.0 30 AATCCCG 50 2.1827873E-11 45.0 1 CGAACTT 20 7.0316816E-4 45.0 34 CGAACGA 45 3.8380676E-10 45.0 27 CGAACAG 40 6.8102963E-9 45.0 1 ACGCCAT 40 6.8102963E-9 45.0 17 GTCGATC 20 7.0316816E-4 45.0 30 GATCGGC 25 3.8894752E-5 45.0 20 GTCGAAT 20 7.0316816E-4 45.0 9 CGAAACT 25 3.8894752E-5 45.0 44 ACGTTGC 45 3.8380676E-10 45.0 33 CGTGCGA 45 3.8380676E-10 45.0 45 CCTAGCG 20 7.0316816E-4 45.0 1 >>END_MODULE