##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548606_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 410606 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.548226280181 31.0 31.0 34.0 30.0 34.0 2 31.73336970234239 31.0 31.0 34.0 30.0 34.0 3 31.922351353852598 33.0 31.0 34.0 30.0 34.0 4 35.63935987296825 37.0 35.0 37.0 33.0 37.0 5 35.36970234239149 37.0 35.0 37.0 33.0 37.0 6 34.039483105458764 37.0 35.0 37.0 32.0 37.0 7 34.76503265904541 37.0 35.0 37.0 32.0 37.0 8 35.40170625855443 37.0 35.0 37.0 32.0 37.0 9 37.064095507615576 39.0 37.0 39.0 33.0 39.0 10 36.62959138444153 38.0 35.0 39.0 32.0 39.0 11 36.61961344938944 39.0 35.0 39.0 32.0 39.0 12 36.500650258398565 38.0 35.0 39.0 32.0 39.0 13 36.4463938666264 38.0 35.0 39.0 32.0 39.0 14 37.56243698338553 39.0 36.0 41.0 32.0 41.0 15 37.59696887040131 39.0 36.0 41.0 32.0 41.0 16 37.51668022386424 39.0 36.0 41.0 32.0 41.0 17 37.42167917663161 39.0 36.0 40.0 32.0 41.0 18 37.21019420076667 39.0 36.0 40.0 32.0 41.0 19 37.027637199651245 39.0 35.0 40.0 32.0 41.0 20 36.908337920049874 39.0 35.0 40.0 31.0 41.0 21 36.848989055201336 39.0 35.0 40.0 31.0 41.0 22 36.824091708353016 39.0 35.0 40.0 31.0 41.0 23 36.58310886835555 38.0 35.0 40.0 31.0 41.0 24 36.41486242285792 38.0 35.0 40.0 30.0 41.0 25 36.45396803748606 38.0 35.0 40.0 31.0 41.0 26 36.34433739399814 38.0 35.0 40.0 30.0 41.0 27 36.29008830850012 38.0 35.0 40.0 30.0 41.0 28 36.139744670073014 38.0 35.0 40.0 30.0 41.0 29 35.941430471059846 38.0 35.0 40.0 30.0 41.0 30 35.92931910395854 38.0 35.0 40.0 30.0 41.0 31 35.810782112292564 38.0 35.0 40.0 29.0 41.0 32 35.69630010277492 38.0 35.0 40.0 29.0 41.0 33 35.60597994184206 38.0 34.0 40.0 28.0 41.0 34 35.43915334895252 38.0 34.0 40.0 27.0 41.0 35 35.326597760383436 38.0 34.0 40.0 27.0 41.0 36 35.24599981490772 38.0 34.0 40.0 26.0 41.0 37 35.12254326532004 38.0 34.0 40.0 26.0 41.0 38 35.0116656843787 38.0 34.0 40.0 26.0 41.0 39 34.85503621476549 37.0 34.0 40.0 25.0 41.0 40 34.675219066452996 37.0 33.0 40.0 24.0 41.0 41 34.55949742575608 37.0 33.0 40.0 24.0 41.0 42 34.46584316839014 37.0 33.0 40.0 24.0 41.0 43 34.40924876889281 37.0 33.0 40.0 23.0 41.0 44 34.253432731133984 37.0 33.0 40.0 23.0 41.0 45 34.05545705615602 36.0 33.0 40.0 23.0 41.0 46 33.83052122959723 36.0 33.0 40.0 22.0 41.0 47 33.72724704461211 36.0 33.0 40.0 22.0 41.0 48 33.56381299834878 36.0 33.0 40.0 22.0 41.0 49 33.43208574643332 36.0 32.0 39.0 21.0 41.0 50 33.300402332162705 35.0 32.0 39.0 20.0 41.0 51 32.14070422741022 35.0 30.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 7.0 11 12.0 12 13.0 13 33.0 14 29.0 15 68.0 16 115.0 17 225.0 18 400.0 19 796.0 20 1200.0 21 1853.0 22 2601.0 23 3250.0 24 3927.0 25 4439.0 26 5007.0 27 5869.0 28 7000.0 29 8594.0 30 10719.0 31 13488.0 32 16762.0 33 21751.0 34 32520.0 35 45595.0 36 40819.0 37 51640.0 38 67596.0 39 64267.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.963907005742733 35.35773953619772 19.207220547191227 25.471132910868327 2 20.74567833884551 37.6769944910693 19.002157786296355 22.57516938378884 3 19.87111732415016 36.12343706618997 20.739833319532593 23.265612290127276 4 20.617818541375428 35.73425619693818 18.452238885939316 25.195686375747066 5 16.09572193294789 41.56222753686015 16.582076248276937 25.75997428191502 6 17.90962625972343 46.32908432901613 17.04553757129706 18.71575183996337 7 74.0675976483539 16.451050398679026 5.309956503314614 4.171395449652465 8 75.93654257365941 13.903596148132271 4.657262680038772 5.502598598169535 9 70.11855647506368 17.180703642908288 7.686687481429886 5.01405240059814 10 34.367982932543605 35.70600527025908 13.23093184220396 16.695079954993354 11 24.04324340121674 35.215754275388086 21.971427597258685 18.76957472613649 12 25.22588564219714 30.642026663029764 26.83521429302056 17.29687340175253 13 23.24832077465989 32.1697685859437 24.478210255086385 20.10370038431002 14 18.92690316264253 34.0655031831001 27.36150957365455 19.646084080602815 15 17.447869734002914 35.81998314686098 25.371036955134606 21.361110164001502 16 19.789043511297937 33.040920006039855 24.866416954452685 22.303619528209527 17 19.419833124698616 32.71871331641525 27.475974535199192 20.38547902368694 18 20.80875583893075 33.18436652167772 24.863494444796228 21.143383194595305 19 20.270526977199555 35.32072108054924 24.212748961291357 20.196002980959847 20 20.159715152725482 35.706492355201824 24.07733934720876 20.056453144863934 21 21.36744226825716 33.61957691801874 25.616040681334418 19.396940132389688 22 20.569110047101113 34.01265446681247 23.476763612806437 21.94147187327998 23 19.357486252027492 34.39818219899368 22.83210669108586 23.41222485789297 24 19.062069234253762 35.92421932460802 23.366925958217855 21.646785482920368 25 19.509943839106104 34.096920161907036 24.01937623902232 22.37375975996454 26 20.888150684597885 34.81878004705241 21.12682230654204 23.16624696180767 27 18.69091050788347 34.081333443739254 23.989176972572245 23.23857907580503 28 20.119774187420543 33.55674296040487 24.7103062303035 21.613176621871087 29 20.298047276464544 34.94128191015231 22.329922115117657 22.43074869826549 30 21.180158107772414 34.62370252748377 22.18330954735196 22.012829817391854 31 22.052283697754053 33.448853645587256 21.763685869178726 22.73517678747997 32 20.443198589402005 33.57671344305733 23.128497878745076 22.851590088795586 33 22.053257867639537 32.683156115595 22.142394412161536 23.121191604603926 34 18.896460353721086 33.31807133846071 23.098298612294997 24.687169695523203 35 21.524770704763206 31.40285334359459 23.928778439672094 23.143597511970114 36 22.596114036326796 32.56990886640721 24.90173061280157 19.932246484464425 37 21.700851911564857 33.82317842408538 23.560055138015517 20.915914526334248 38 22.20084460529072 32.81125945553645 24.904896664929396 20.082999274243434 39 20.924682055303627 31.542646722161876 26.64939138736404 20.883279835170455 40 21.42905851351417 31.10767986829223 26.346668095449168 21.11659352274443 41 19.91909519101036 29.553878900941534 28.341280935982425 22.18574497206568 42 19.652659727329848 31.824181819067427 26.0215388961681 22.501619557434623 43 20.176519583250123 30.13911145964745 26.53541351076214 23.14895544634029 44 21.124873966771066 29.780129856845733 24.748785940780213 24.346210235602985 45 22.050091815511706 30.94889017695796 25.925826704919068 21.075191302611263 46 20.04208413905301 31.588432706779734 27.29258705425639 21.076896099910865 47 21.5839515253065 31.201930804713037 25.885155112200014 21.32896255778045 48 20.23277789413696 30.0606907838658 27.634033599119352 22.07249772287789 49 20.570571301929345 29.986653872568837 27.35128078985694 22.091494035644878 50 19.53600288354286 29.172004305830896 26.63672717885272 24.655265631773528 51 20.014076754845277 28.29622557877868 27.734860182267184 23.954837484108854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20489.0 1 15018.5 2 9548.0 3 5925.0 4 2302.0 5 1871.5 6 1441.0 7 1402.0 8 1363.0 9 1467.5 10 1572.0 11 1620.0 12 1668.0 13 1664.0 14 1660.0 15 1601.0 16 1542.0 17 1692.5 18 1843.0 19 1833.0 20 1823.0 21 1928.0 22 2033.0 23 1948.0 24 1863.0 25 2433.5 26 3697.5 27 4391.0 28 4679.0 29 4967.0 30 5778.5 31 6590.0 32 7229.0 33 7868.0 34 10949.0 35 14030.0 36 13058.5 37 12087.0 38 11773.5 39 11460.0 40 13166.5 41 14873.0 42 16527.5 43 18182.0 44 21908.5 45 25635.0 46 28148.0 47 30661.0 48 31842.0 49 33023.0 50 30273.0 51 27523.0 52 26029.0 53 24535.0 54 22535.5 55 20536.0 56 19683.5 57 18831.0 58 18217.0 59 17603.0 60 16196.5 61 14790.0 62 13847.0 63 12904.0 64 11676.0 65 10448.0 66 9216.5 67 7985.0 68 7197.5 69 6410.0 70 5460.5 71 4511.0 72 3567.5 73 2624.0 74 2591.0 75 1970.0 76 1382.0 77 1231.0 78 1080.0 79 729.0 80 378.0 81 331.5 82 285.0 83 197.0 84 109.0 85 120.5 86 132.0 87 79.0 88 26.0 89 20.5 90 15.0 91 17.0 92 19.0 93 11.0 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 410606.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.84530315216252 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68648512821743 19.549895769886895 2 9.068668989425511 4.506276604577843 3 3.359792461716684 2.504251867191042 4 1.7299145836630663 1.7192100903382357 5 1.0652802782607302 1.3233605727703943 6 0.7025224810874862 1.0472630408296772 7 0.5256425263265674 0.9141823541376493 8 0.34376620471227504 0.6832780455635866 9 0.2947995062013809 0.6591944790613011 >10 2.6122234204411106 15.130934061587254 >50 0.926369973505938 16.72889099801167 >100 0.6735419224843605 26.592476332661107 >500 0.0029979610793072426 0.4210899571466621 >1k 0.006995242518383566 3.811410337357553 >5k 0.0 0.0 >10k+ 9.993203597690808E-4 4.408285488879119 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17755 4.324096579202448 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3834 0.9337418352386473 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2883 0.7021329449642723 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1966 0.4788044987165312 No Hit CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT 1937 0.4717417670467553 TruSeq Adapter, Index 15 (95% over 23bp) AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT 1888 0.45980818594954775 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC 1610 0.3921033789082478 No Hit AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT 1233 0.30028786720116124 No Hit AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT 632 0.15391884190684013 No Hit AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT 542 0.1320000194833977 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 522 0.12712917005596605 No Hit AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 472 0.11495204648738694 No Hit ATCCTAGCTGAGTAATTTCTGCTTCCTAGAACCATTGGTTATTACACTTTT 460 0.11202953683092795 No Hit ACAGCAGAGGGATGAATGCCGCTGCCCAGCTTGCTTTTTTGTTTGTTTGTT 459 0.11178599435955636 No Hit TTCGAAGGGGGAATATGGGAAGAAAACTGAAAATCATGGAAATTGAGAAAC 454 0.11056828200269844 No Hit AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT 436 0.10618451751800996 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.87084942743165E-4 0.0 0.0 0.628826661081426 0.0 2 4.87084942743165E-4 0.0 0.0 0.7873728099443262 0.0 3 4.87084942743165E-4 0.0 0.0 1.040656980170772 0.0 4 4.87084942743165E-4 0.0 0.0 2.245705128517362 0.0 5 4.87084942743165E-4 0.0 0.0 2.444679327627945 0.0 6 4.87084942743165E-4 0.0 0.0 3.402044782589636 0.0 7 4.87084942743165E-4 0.0 0.0 4.2084139053009455 0.0 8 4.87084942743165E-4 0.0 0.0 4.790723954350399 0.0 9 4.87084942743165E-4 0.0 0.0 6.089292411703677 0.0 10 4.87084942743165E-4 0.0 0.0 7.32551399638583 0.0 11 4.87084942743165E-4 0.0 0.0 8.675713457669882 0.0 12 4.87084942743165E-4 0.0 0.0 9.182281798122775 0.0 13 4.87084942743165E-4 0.0 0.0 9.43702722317745 0.0 14 4.87084942743165E-4 0.0 0.0 9.719536489968485 0.0 15 4.87084942743165E-4 0.0 0.0 9.966732098410642 0.0 16 4.87084942743165E-4 0.0 0.0 10.375152822900786 0.0 17 4.87084942743165E-4 0.0 0.0 10.941876153782458 0.0 18 4.87084942743165E-4 0.0 0.0 11.854673336483149 0.0 19 4.87084942743165E-4 0.0 0.0 12.151308066613737 0.0 20 4.87084942743165E-4 0.0 0.0 12.490806271705722 0.0 21 4.87084942743165E-4 0.0 0.0 12.780134727695163 0.0 22 4.87084942743165E-4 0.0 0.0 13.081640307253181 0.0 23 4.87084942743165E-4 0.0 0.0 13.398976147450353 0.0 24 4.87084942743165E-4 0.0 0.0 13.639109024222734 0.0 25 4.87084942743165E-4 0.0 0.0 13.834917171205486 0.0 26 4.87084942743165E-4 0.0 0.0 14.024393213932578 0.0 27 4.87084942743165E-4 0.0 0.0 14.262334208462613 0.0 28 4.87084942743165E-4 0.0 0.0 14.485662654710355 0.0 29 4.87084942743165E-4 0.0 0.0 14.706799218715751 0.0 30 4.87084942743165E-4 0.0 0.0 14.970312172739805 0.0 31 4.87084942743165E-4 0.0 0.0 15.175618476106049 0.0 32 4.87084942743165E-4 0.0 0.0 15.396267955168701 0.0 33 4.87084942743165E-4 0.0 0.0 15.602548428420432 0.0 34 4.87084942743165E-4 0.0 0.0 15.800304915174157 0.0 35 4.87084942743165E-4 0.0 0.0 16.021685021650924 0.0 36 4.87084942743165E-4 0.0 0.0 16.21944150840465 0.0 37 4.87084942743165E-4 0.0 0.0 16.422799471999923 0.0 38 4.87084942743165E-4 0.0 0.0 16.647832715547263 0.0 39 4.87084942743165E-4 0.0 0.0 16.872135331680493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCACTA 70 0.0 45.000004 44 CGAACAG 35 1.2093733E-7 45.000004 1 TACTTAG 35 1.2093733E-7 45.000004 1 TACCAGC 35 1.2093733E-7 45.000004 17 CGTGCCG 30 2.16208E-6 45.000004 27 TAGACGC 30 2.16208E-6 45.000004 28 TCCGAAC 35 1.2093733E-7 45.000004 35 TATGGGC 60 0.0 45.000004 4 ATTACTC 65 0.0 45.000004 30 CGGCACG 35 1.2093733E-7 45.000004 1 ACATTAC 35 1.2093733E-7 45.000004 29 TAAGTAA 30 2.16208E-6 45.000004 14 TGCGGTC 30 2.16208E-6 45.000004 10 ACCCTAT 35 1.2093733E-7 45.000004 29 GTAGACG 30 2.16208E-6 45.000004 27 CCTCATA 35 1.2093733E-7 45.000004 10 CGCTACA 35 1.2093733E-7 45.000004 30 TGTTCGG 30 2.16208E-6 45.000004 2 GAAACGT 30 2.16208E-6 45.000004 19 AACGCCT 30 2.16208E-6 45.000004 32 >>END_MODULE