##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518191
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57043059412456	31.0	31.0	34.0	30.0	34.0
2	31.784673990864373	31.0	31.0	34.0	30.0	34.0
3	31.956770765991692	33.0	31.0	34.0	30.0	34.0
4	35.70090179103844	37.0	35.0	37.0	33.0	37.0
5	35.46611577584327	37.0	35.0	37.0	33.0	37.0
6	32.499966228668576	37.0	35.0	37.0	25.0	37.0
7	34.128338778558486	37.0	35.0	37.0	26.0	37.0
8	35.286593167384225	37.0	35.0	37.0	32.0	37.0
9	37.163951129988746	39.0	37.0	39.0	34.0	39.0
10	36.57574909637566	38.0	35.0	39.0	32.0	39.0
11	36.628854997481625	39.0	35.0	39.0	32.0	39.0
12	36.721932646456615	39.0	35.0	39.0	32.0	39.0
13	36.733675420839035	39.0	37.0	39.0	32.0	39.0
14	37.844954852554366	40.0	37.0	41.0	33.0	41.0
15	37.872666642222654	40.0	37.0	41.0	33.0	41.0
16	37.816866753764536	40.0	37.0	41.0	33.0	41.0
17	37.76881883321015	39.0	37.0	41.0	33.0	41.0
18	37.59573400541499	39.0	36.0	41.0	33.0	41.0
19	37.52894975018864	39.0	36.0	41.0	32.0	41.0
20	37.391847793574186	39.0	36.0	41.0	32.0	41.0
21	37.35848750750206	39.0	35.0	41.0	32.0	41.0
22	37.28695210839246	39.0	35.0	41.0	32.0	41.0
23	37.099048420370096	39.0	35.0	40.0	32.0	41.0
24	36.9925085537958	39.0	35.0	40.0	31.0	41.0
25	37.02512008120558	39.0	35.0	40.0	31.0	41.0
26	36.951228408058036	39.0	35.0	40.0	31.0	41.0
27	36.892460502015666	39.0	35.0	40.0	31.0	41.0
28	36.80017406709109	39.0	35.0	40.0	31.0	41.0
29	36.63495892441204	39.0	35.0	40.0	31.0	41.0
30	36.65990339469423	39.0	35.0	40.0	31.0	41.0
31	36.22805876597625	38.0	35.0	40.0	30.0	41.0
32	36.30165518119767	38.0	35.0	40.0	30.0	41.0
33	36.281770621257415	38.0	35.0	40.0	30.0	41.0
34	36.20128678421663	38.0	35.0	40.0	30.0	41.0
35	36.171703097892475	38.0	35.0	40.0	30.0	41.0
36	36.099725776788866	38.0	35.0	40.0	30.0	41.0
37	36.01449272565521	38.0	35.0	40.0	30.0	41.0
38	35.90889652657032	38.0	35.0	40.0	29.0	41.0
39	35.84446082622045	38.0	35.0	40.0	29.0	41.0
40	35.74379717131328	38.0	35.0	40.0	28.0	41.0
41	35.7174593923862	38.0	35.0	40.0	28.0	41.0
42	35.593078613870176	38.0	35.0	40.0	28.0	41.0
43	35.52348072428892	38.0	35.0	40.0	27.0	41.0
44	35.319691001966454	38.0	34.0	40.0	26.0	41.0
45	35.09268783132088	38.0	34.0	40.0	26.0	41.0
46	34.86099719987418	38.0	34.0	40.0	25.0	41.0
47	34.837718138678596	38.0	34.0	40.0	25.0	41.0
48	34.836535177183706	38.0	34.0	40.0	25.0	41.0
49	34.69383875829568	37.0	33.0	40.0	25.0	41.0
50	34.5623949470369	37.0	33.0	40.0	24.0	41.0
51	33.518552039691926	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	4.0
13	3.0
14	14.0
15	29.0
16	76.0
17	183.0
18	316.0
19	707.0
20	1147.0
21	1708.0
22	2351.0
23	2953.0
24	3772.0
25	4225.0
26	4889.0
27	5765.0
28	6983.0
29	8699.0
30	11226.0
31	14259.0
32	18741.0
33	25118.0
34	35516.0
35	50814.0
36	54310.0
37	71750.0
38	96014.0
39	96587.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.526952417158924	33.691052141005926	21.0462937411109	26.735701700724253
2	22.529723596125752	34.016414796860616	19.610143750084426	23.843717856929203
3	18.396498588358345	36.30514617197134	20.036241463089866	25.262113776580453
4	18.22667703607357	37.07783423486707	19.299447501017962	25.396041228041398
5	16.72510715161012	38.97848476719974	16.02883878724254	28.2675692939476
6	16.353622505987175	46.29875856585699	16.679178140878555	20.66844078727728
7	82.60486963301177	11.326711579321138	3.2918364078110196	2.7765823798560763
8	83.95552991078578	10.35930766840798	2.56970885252735	3.1154535682788778
9	77.30354251617648	14.6723891383679	5.007806002033998	3.016262343421634
10	28.585019809298117	49.19807561304616	10.026997767232546	12.189906810423183
11	16.96613796843249	32.520634283497785	33.08548392388135	17.42774382418838
12	22.69954514841053	29.48410914122399	30.1859738976555	17.630371812709985
13	22.07274923725036	30.750437579965688	27.46979395628253	19.707019226501423
14	18.39283198666129	33.686227665088744	28.1828514968419	19.738088851408072
15	16.90380573958251	37.11160556628734	26.52342476036828	19.461163933761874
16	19.695247505263502	34.26748052359072	26.574950163163773	19.462321807982
17	18.213940419652214	35.638789558290284	26.876962355579316	19.27030766647819
18	18.529847102709233	33.65728080958566	27.61259844343109	20.200273644274024
19	18.637529405180715	36.56180829076538	25.420163607627305	19.380498696426606
20	19.3745163462893	35.83099667883078	27.14558917464796	17.64889780023196
21	19.88243717085013	36.00969526680316	26.696526956276738	17.411340606069963
22	20.735211533971064	32.06288800847564	26.318674002443114	20.883226455110183
23	17.579425347024554	36.31151448018202	25.062187494572463	21.046872678220964
24	16.31946521649353	36.44119639283584	27.63421209554006	19.60512629513056
25	18.20236167745098	36.31402320765895	25.05890685094878	20.424708263941287
26	20.026978469328878	35.867276737727984	23.81149035780243	20.29425443514071
27	17.35923626616441	34.641087938617225	25.482109878403907	22.517565916814455
28	16.768141476791378	36.65598206066875	27.176851778591292	19.399024683948582
29	18.64852921027189	35.566615398569255	24.87615570320596	20.908699687952897
30	19.225729509003436	34.367057706521344	26.143256058094412	20.263956726380812
31	20.40830504582287	35.35974187124053	23.491145156901606	20.740807926034996
32	20.206641952484702	33.9909415640179	24.12604618760264	21.676370295894756
33	18.786895179576643	34.17735931345778	23.954101865914307	23.081643641051272
34	16.82198262802712	35.114272536574354	27.08634461038497	20.977400225013557
35	18.25813261905359	34.46489807812178	25.85320856595348	21.423760736871152
36	18.75621151274337	36.82889127754052	24.043837117973872	20.371060091742237
37	17.563215107942824	36.71252491841811	24.801086857934624	20.92317311570444
38	18.1778533397917	35.5764573294403	25.914575899620022	20.331113431147973
39	18.03061033479933	33.104009911403324	26.31828804436974	22.547091709427605
40	20.110924350287828	33.861838588474136	25.0419246957203	20.985312365517732
41	17.9871900515447	32.55537051010149	27.749227601405657	21.708211836948152
42	19.508829755823626	32.56308967156898	25.359375211070823	22.568705361536576
43	18.8389995194822	33.80162912902772	26.12588794479256	21.23348340669753
44	19.596249259442946	32.2103239925047	24.515863841710875	23.677562906341485
45	19.767807623057905	33.87303137260199	26.29879716166433	20.06036384267577
46	18.57133759559699	33.209183486397876	26.831805260994496	21.387673657010637
47	18.04952228039468	33.43921449812907	26.73087722480707	21.78038599666918
48	18.55589927266201	32.433600737951835	28.07169557171004	20.938804417676106
49	18.65412560233582	32.14393920388428	27.89550571121459	21.30642948256531
50	18.108380886584293	30.860049672804042	28.064362368315933	22.967207072295736
51	17.82547361880079	30.563247914379062	28.496249452421985	23.115029014398168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17125.0
1	13043.5
2	8962.0
3	5649.5
4	2337.0
5	2035.0
6	1733.0
7	1940.5
8	2148.0
9	2454.5
10	2761.0
11	2966.5
12	3172.0
13	3432.5
14	3693.0
15	3821.5
16	3950.0
17	3758.5
18	3567.0
19	3644.0
20	3721.0
21	3727.0
22	3733.0
23	3712.0
24	3691.0
25	4329.5
26	5663.5
27	6359.0
28	9046.5
29	11734.0
30	11697.0
31	11660.0
32	13353.0
33	15046.0
34	16841.0
35	18636.0
36	20226.5
37	21817.0
38	22486.0
39	23155.0
40	24021.0
41	24887.0
42	26202.0
43	27517.0
44	30349.0
45	33181.0
46	35585.5
47	37990.0
48	39650.0
49	41310.0
50	42270.0
51	43230.0
52	39445.0
53	35660.0
54	30890.5
55	26121.0
56	22571.0
57	19021.0
58	17332.5
59	15644.0
60	13856.5
61	12069.0
62	10338.5
63	8608.0
64	7350.5
65	6093.0
66	5471.0
67	4849.0
68	3731.0
69	2613.0
70	2213.0
71	1813.0
72	1560.0
73	1307.0
74	1182.0
75	809.5
76	562.0
77	423.5
78	285.0
79	194.5
80	104.0
81	103.5
82	103.0
83	111.0
84	119.0
85	78.0
86	37.0
87	28.0
88	19.0
89	17.0
90	15.0
91	11.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.300574629600288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63782745875233	21.688945554468507
2	8.92974477174696	5.0543381667221565
3	3.4456858256044596	2.925446665730247
4	1.929258669211858	2.1839651579133412
5	1.2361865888137589	1.7492395406417394
6	0.9152961333474031	1.5542043917989656
7	0.6742377305857326	1.33569206487737
8	0.5804466098179392	1.3141578079720855
9	0.4469537861346613	1.1384144082437753
>10	4.1027176108401955	24.480950939007677
>50	0.7031187597600738	13.819212313441188
>100	0.3916191913398447	17.305583509550512
>500	0.003453432022397219	0.6528638593297345
>1k	0.002762745617917775	1.8913505098426677
>5k	0.0	0.0
>10k+	6.906864044794438E-4	2.9056351104600306
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14865	2.8686333803558917	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3435	0.6628829910206855	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2736	0.5279906443763014	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	1755	0.33867820938611437	TruSeq Adapter, Index 13 (96% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1750	0.33771331420267814	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	785	0.15148854379948706	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGT	739	0.14261150811187379	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	678	0.1308397868739519	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	595	0.11482252682891057	No Hit
AGCTAGGGGGTCTCTTGCTTCTTGCTCTCTTGCTTCTTGCTCTCTTGCTTC	543	0.10478761692117385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9297903668724466E-4	0.0	0.0	0.39927362690590923	0.0
2	1.9297903668724466E-4	0.0	0.0	0.5235521265324947	0.0
3	1.9297903668724466E-4	0.0	0.0	0.6883562238634017	0.0
4	1.9297903668724466E-4	0.0	0.0	1.4564127898786354	0.0
5	1.9297903668724466E-4	0.0	0.0	1.5770246878081633	0.0
6	1.9297903668724466E-4	0.0	0.0	2.3273271824481707	0.0
7	1.9297903668724466E-4	0.0	0.0	2.9195798460413247	0.0
8	1.9297903668724466E-4	0.0	0.0	3.442938993537132	0.0
9	1.9297903668724466E-4	0.0	0.0	4.306713161749239	0.0
10	1.9297903668724466E-4	0.0	0.0	5.428693281048879	0.0
11	1.9297903668724466E-4	0.0	0.0	6.5126565301211325	0.0
12	1.9297903668724466E-4	0.0	0.0	7.002051367159986	0.0
13	1.9297903668724466E-4	0.0	0.0	7.210661705818897	0.0
14	1.9297903668724466E-4	0.0	0.0	7.4154124637440635	0.0
15	1.9297903668724466E-4	0.0	0.0	7.591988282312892	0.0
16	1.9297903668724466E-4	0.0	0.0	7.97177102651339	0.0
17	1.9297903668724466E-4	0.0	0.0	8.528322568319403	0.0
18	1.9297903668724466E-4	0.0	0.0	9.177890005808669	0.0
19	1.9297903668724466E-4	0.0	0.0	9.483761778957952	0.0
20	3.859580733744893E-4	0.0	0.0	9.779791621236186	0.0
21	3.859580733744893E-4	0.0	0.0	10.104575339980817	0.0
22	3.859580733744893E-4	0.0	0.0	10.51272600257434	0.0
23	3.859580733744893E-4	0.0	0.0	10.907947069709817	0.0
24	3.859580733744893E-4	0.0	0.0	11.178310700108646	0.0
25	3.859580733744893E-4	0.0	0.0	11.422236202481324	0.0
26	3.859580733744893E-4	0.0	0.0	11.664424893523817	0.0
27	3.859580733744893E-4	0.0	0.0	11.916455515437358	0.0
28	3.859580733744893E-4	0.0	0.0	12.19164362175337	0.0
29	3.859580733744893E-4	0.0	0.0	12.489410275361788	0.0
30	3.859580733744893E-4	0.0	0.0	12.835421688142016	0.0
31	3.859580733744893E-4	0.0	0.0	13.115820228448584	0.0
32	3.859580733744893E-4	0.0	0.0	13.400657286598957	0.0
33	3.859580733744893E-4	0.0	0.0	13.6798979526854	0.0
34	3.859580733744893E-4	0.0	0.0	13.953156268634539	0.0
35	3.859580733744893E-4	0.0	0.0	14.25767718852701	0.0
36	3.859580733744893E-4	0.0	0.0	14.539619561127076	0.0
37	3.859580733744893E-4	0.0	0.0	14.819825122396955	0.0
38	3.859580733744893E-4	0.0	0.0	15.129363497243295	0.0
39	3.859580733744893E-4	0.0	0.0	15.452989341767804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCC	20	7.0296956E-4	45.000004	18
AATCCGA	20	7.0296956E-4	45.000004	14
CCTTACG	35	1.2101918E-7	45.000004	1
TACCCGT	20	7.0296956E-4	45.000004	42
ACGCCTA	35	1.2101918E-7	45.000004	27
GCGACCG	65	0.0	45.000004	32
CGTATAA	35	1.2101918E-7	45.000004	17
GTCGAGA	20	7.0296956E-4	45.000004	27
CGAAATA	20	7.0296956E-4	45.000004	30
CCGATTC	20	7.0296956E-4	45.000004	44
ACCGCGC	65	0.0	45.000004	35
GACGTAA	20	7.0296956E-4	45.000004	32
ACTCTAC	30	2.1631859E-6	45.000004	35
TTGTCGA	20	7.0296956E-4	45.000004	25
TCTTACG	60	0.0	45.000004	1
TTGTCCG	80	0.0	45.000004	12
CTCCCGT	20	7.0296956E-4	45.000004	44
TTCCGGT	30	2.1631859E-6	45.000004	45
CGATTGT	35	1.2101918E-7	45.000004	24
CCGCTTA	20	7.0296956E-4	45.000004	25
>>END_MODULE