FastQCFastQC Report
Sat 18 Jun 2016
SRR3548602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455406
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237895.223690509128119No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56281.2358203449229919No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44120.9688058567519972No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24630.5408360891160855No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT19370.4253347562394874Illumina Single End Adapter 1 (95% over 24bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.2083854846005542No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.1728128307488263No Hit
TTTAAAGGGATGGGTGTTAGAGCTTAATGTATAAATTAGATACTGTAAGAG5590.12274761421676482No Hit
AAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT5460.11989301853730518Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT5280.11594050144266874No Hit
TTGAACGGGATTGAAATTGCTATGTGATGTTGTTAAAAGAGTTTGAAAATG5000.10979214151767873No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.10627879298911302No Hit
AATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT4770.1047417030078655Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGT302.1626038E-645.00000422
CACGCTT302.1626038E-645.00000427
CACGACC302.1626038E-645.00000427
GCACCTA600.045.0000049
CCCACGG302.1626038E-645.0000042
ATACTAC302.1626038E-645.00000440
TAGTTCG600.045.0000041
TACGACG302.1626038E-645.0000041
CGCTACT302.1626038E-645.00000429
AACGCCA302.1626038E-645.00000414
TAGGGCG302.1626038E-645.0000045
AGTTCGG600.045.0000042
GAGCCGT302.1626038E-645.0000048
CCAAGCG302.1626038E-645.0000041
CGCACCC302.1626038E-645.00000431
ACATCGG1050.045.0000042
CGGTATC302.1626038E-645.00000427
ATTGCCG302.1626038E-645.00000412
CGCCGAC302.1626038E-645.00000435
TGTCGCC302.1626038E-645.00000424
Produced by FastQC (version 0.11.5)