##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455406
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62128518289175	31.0	31.0	34.0	30.0	34.0
2	31.806787789357188	33.0	31.0	34.0	30.0	34.0
3	32.00373073696877	33.0	31.0	34.0	30.0	34.0
4	35.73630782203133	37.0	35.0	37.0	35.0	37.0
5	35.480962042660835	37.0	35.0	37.0	33.0	37.0
6	34.10672454908367	37.0	35.0	37.0	32.0	37.0
7	34.90428979855338	37.0	35.0	37.0	32.0	37.0
8	35.57272631454131	37.0	35.0	37.0	32.0	37.0
9	37.22992670276632	39.0	37.0	39.0	34.0	39.0
10	36.71553075717053	38.0	37.0	39.0	32.0	39.0
11	36.72944361734365	39.0	37.0	39.0	32.0	39.0
12	36.80904731162962	39.0	37.0	39.0	32.0	39.0
13	36.78729529255214	39.0	37.0	39.0	32.0	39.0
14	37.95058914463139	40.0	37.0	41.0	33.0	41.0
15	37.90237721944814	40.0	37.0	41.0	33.0	41.0
16	37.832964431737835	40.0	37.0	41.0	33.0	41.0
17	37.73537898051409	39.0	37.0	41.0	33.0	41.0
18	37.4701804543638	39.0	36.0	40.0	33.0	41.0
19	37.22798338186146	39.0	35.0	40.0	32.0	41.0
20	37.10244265556449	39.0	35.0	40.0	32.0	41.0
21	37.09109234397439	39.0	35.0	40.0	32.0	41.0
22	37.04736432985073	39.0	35.0	40.0	32.0	41.0
23	36.83002419818799	39.0	35.0	40.0	31.0	41.0
24	36.67259983399428	38.0	35.0	40.0	31.0	41.0
25	36.71679117095515	38.0	35.0	40.0	31.0	41.0
26	36.641456195131376	38.0	35.0	40.0	31.0	41.0
27	36.58868350438949	38.0	35.0	40.0	31.0	41.0
28	36.47370697794935	38.0	35.0	40.0	31.0	41.0
29	36.309168082985295	38.0	35.0	40.0	30.0	41.0
30	36.28032349156577	38.0	35.0	40.0	30.0	41.0
31	36.14442277879518	38.0	35.0	40.0	30.0	41.0
32	36.03703508517675	38.0	35.0	40.0	30.0	41.0
33	35.861589438874326	38.0	35.0	40.0	30.0	41.0
34	35.80496304396517	38.0	35.0	40.0	30.0	41.0
35	35.62470850186427	38.0	35.0	40.0	28.0	41.0
36	35.519431013205796	38.0	35.0	40.0	28.0	41.0
37	35.49015603659152	38.0	35.0	40.0	28.0	41.0
38	35.342103529597765	38.0	34.0	40.0	27.0	41.0
39	35.250567625371644	38.0	34.0	40.0	27.0	41.0
40	35.09411162786613	38.0	34.0	40.0	26.0	41.0
41	34.97258051057737	38.0	34.0	40.0	25.0	41.0
42	34.84472536593721	38.0	34.0	40.0	24.0	41.0
43	34.71822505632337	37.0	33.0	40.0	24.0	41.0
44	34.60078040254191	37.0	33.0	40.0	24.0	41.0
45	34.4822905275732	37.0	33.0	40.0	23.0	41.0
46	34.3302130406714	37.0	33.0	40.0	23.0	41.0
47	34.21737526514802	37.0	33.0	40.0	23.0	41.0
48	34.08505377619092	37.0	33.0	40.0	23.0	41.0
49	33.887175399533604	36.0	33.0	40.0	22.0	41.0
50	33.757392304888384	36.0	33.0	40.0	22.0	41.0
51	32.77009964734764	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	3.0
13	6.0
14	21.0
15	26.0
16	84.0
17	178.0
18	367.0
19	674.0
20	1141.0
21	1801.0
22	2488.0
23	3218.0
24	3980.0
25	4501.0
26	5053.0
27	5597.0
28	6687.0
29	8097.0
30	10311.0
31	13037.0
32	16968.0
33	22583.0
34	32216.0
35	52190.0
36	47706.0
37	59581.0
38	80425.0
39	76447.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.31177894011058	36.65103226571455	19.925736595477442	24.111452198697425
2	20.877854046718753	38.92921920220638	17.958481003763673	22.23444574731119
3	18.918283904911224	38.03902451878105	19.208135158517894	23.83455641778984
4	17.381413507946757	40.59564432616171	18.315964216545236	23.706977949346296
5	15.70203291129234	42.791926325081356	14.551850436753138	26.95419032687316
6	17.15041084219356	47.008603312209324	15.52241296776942	20.318572877827695
7	76.7903804517288	16.29908257686548	3.671888380917247	3.2386485904884874
8	78.33779089427895	15.730798452369973	2.9870050021299677	2.9444056512211083
9	71.45667821679996	20.241279210199252	5.459524029108093	2.842518543892702
10	27.049709489993546	51.484170169035984	9.759203875223427	11.706916465747048
11	16.095088777925632	37.56208745602825	29.871806695563958	16.471017070482162
12	21.539900660070355	33.231885394570995	28.63862136203739	16.589592583321256
13	22.789993983390644	33.6846681861899	26.62437473375406	16.900963096665393
14	17.229680768369324	38.7430117301924	27.448035379419682	16.579272122018594
15	15.299974089054603	41.62176168078594	25.76711769278402	17.31114653737544
16	18.278195719863156	40.10531262214376	24.080710399072476	17.53578125892061
17	17.99471241046451	37.19230752339671	26.139532636811985	18.6734474293268
18	18.596373345981387	37.63784403367544	25.62153331313158	18.144249307211588
19	17.663798895930224	42.20387961511267	24.38615213677466	15.746169352182449
20	17.47715225535017	41.580040667009214	24.034378115352016	16.908428962288596
21	19.402028080438114	39.03044755668568	25.486049810498763	16.08147455237744
22	18.074860673772413	37.68681132879233	25.11648946215026	19.121838535285
23	16.956078751707267	39.48652411255012	24.715089392761623	18.84230774298099
24	16.779752572429878	40.980136405756625	24.27218789387931	17.967923127934196
25	17.578380609829473	40.482778004681535	23.25990434908631	18.678937036402683
26	18.05224349261977	41.21091948722678	22.61849865834003	18.118338361813414
27	16.221788909237034	39.45248854867964	24.4447811403451	19.880941401738227
28	16.901402265231464	39.48476743828584	26.505579636631925	17.10825065985077
29	18.273364865636378	39.00277993702323	24.65755831060636	18.066296886734033
30	19.265666240673156	39.39254203941099	24.41579601498443	16.925995704931424
31	18.31025502518632	40.78119304532659	22.138706999907775	18.76984492957932
32	19.995125228916617	38.32536242385915	24.129897278472395	17.54961506875184
33	18.917844736345153	39.572381567216944	24.176449146475893	17.33332454996201
34	19.156532852004585	37.70262139717088	25.317189496844573	17.823656253979966
35	18.152813094249968	38.41912491271525	25.596500704865548	17.831561288169237
36	20.380495645643666	38.31833572680202	25.575859782260224	15.725308845294089
37	20.663539786476242	39.43009095181003	22.880682292284245	17.025686969429476
38	20.381154398492775	38.931854213602804	23.997048787236004	16.689942600668413
39	20.784091557862656	37.56208745602825	23.297892430051427	18.35592855605767
40	21.10270835254696	38.73883962881472	23.047346763108084	17.11110525553023
41	20.089107302055748	36.00984615925131	26.56838074158004	17.332665797112906
42	21.136743916417437	36.1532346960734	24.750442462330316	17.95957892517885
43	18.773797446673957	36.507204560326386	25.218156985195627	19.500841007804027
44	20.39696446687132	35.52829782655477	24.401962205153204	19.672775501420713
45	21.131693477907625	36.26785769181785	25.350566307865947	17.249882522408576
46	19.445066599913044	35.54849958059402	26.76798285485918	18.238450964633756
47	19.30541099590256	37.7480753437592	24.678199233211686	18.268314427126565
48	20.316157450714307	35.992279416608476	25.73154503893229	17.96001809374492
49	19.940887911006882	35.454956676020956	25.252851301915214	19.351304111056947
50	18.8541652942649	34.60494591639109	25.1428395761145	21.398049213229513
51	19.338787806923932	34.457165693908294	26.668511174644166	19.53553532452361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27078.0
1	20833.5
2	14589.0
3	8967.0
4	3345.0
5	2668.0
6	1991.0
7	2171.5
8	2352.0
9	2857.5
10	3363.0
11	3926.0
12	4489.0
13	4753.0
14	5017.0
15	4991.5
16	4966.0
17	4742.0
18	4518.0
19	4303.0
20	4088.0
21	4258.5
22	4429.0
23	4290.0
24	4151.0
25	4103.0
26	4375.5
27	4696.0
28	6023.5
29	7351.0
30	8030.5
31	8710.0
32	10601.0
33	12492.0
34	15258.0
35	18024.0
36	17556.5
37	17089.0
38	17440.5
39	17792.0
40	17551.5
41	17311.0
42	20192.0
43	23073.0
44	25295.5
45	27518.0
46	29559.0
47	31600.0
48	34772.0
49	37944.0
50	35898.0
51	33852.0
52	30266.5
53	26681.0
54	23522.0
55	20363.0
56	18198.5
57	16034.0
58	14497.0
59	12960.0
60	11409.0
61	9858.0
62	8921.0
63	7984.0
64	6524.5
65	5065.0
66	4388.0
67	3711.0
68	3040.5
69	2370.0
70	1989.5
71	1609.0
72	1167.5
73	726.0
74	701.5
75	664.5
76	652.0
77	518.0
78	384.0
79	253.5
80	123.0
81	137.0
82	151.0
83	104.5
84	58.0
85	58.5
86	59.0
87	44.0
88	29.0
89	18.0
90	7.0
91	8.5
92	10.0
93	8.5
94	7.0
95	5.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.022468117631878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.40035064510515	22.46349208891092
2	9.213654432643768	5.348059840365628
3	3.346208222327949	2.9134566434241163
4	1.8021540479158111	2.0921183359479145
5	1.1535838175647208	1.6739924783144091
6	0.7854886155365772	1.3678090980703872
7	0.633238154081807	1.2864693896395114
8	0.5002892162720379	1.1615702263080208
9	0.4311858797998945	1.126267060433894
>10	3.7902664063911686	23.54342886052413
>50	0.6065332818395299	12.310488339320424
>100	0.32945612555488857	15.548223662166189
>500	0.003795577483351251	0.7422380445473182
>1k	0.0022773464900107507	1.9414074350106763
>5k	7.591154966702502E-4	1.2399274902678263
>10k+	7.591154966702502E-4	5.241051006748636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23789	5.223690509128119	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5628	1.2358203449229919	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4412	0.9688058567519972	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2463	0.5408360891160855	No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	1937	0.4253347562394874	Illumina Single End Adapter 1 (95% over 24bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	949	0.2083854846005542	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	787	0.1728128307488263	No Hit
TTTAAAGGGATGGGTGTTAGAGCTTAATGTATAAATTAGATACTGTAAGAG	559	0.12274761421676482	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	546	0.11989301853730518	Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT	528	0.11594050144266874	No Hit
TTGAACGGGATTGAAATTGCTATGTGATGTTGTTAAAAGAGTTTGAAAATG	500	0.10979214151767873	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	484	0.10627879298911302	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	477	0.1047417030078655	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5017500867357918	0.0
2	0.0	0.0	0.0	0.6769783445980071	0.0
3	0.0	0.0	0.0	0.8653816594423438	0.0
4	0.0	0.0	0.0	1.864929315819291	0.0
5	0.0	0.0	0.0	2.0311546180770566	0.0
6	0.0	0.0	0.0	2.9661444952416085	0.0
7	0.0	0.0	0.0	3.678915077974379	0.0
8	0.0	0.0	0.0	4.176493063332499	0.0
9	0.0	0.0	0.0	5.039678879944489	0.0
10	0.0	0.0	0.0	6.323588182852224	0.0
11	0.0	0.0	0.0	7.583562798909106	0.0
12	0.0	0.0	0.0	8.225188073938419	0.0
13	0.0	0.0	0.0	8.489787134996025	0.0
14	0.0	0.0	0.0	8.757460376016127	0.0
15	0.0	0.0	0.0	8.972872557673812	0.0
16	0.0	0.0	0.0	9.442343754803407	0.0
17	0.0	0.0	0.0	10.052129308792594	0.0
18	0.0	0.0	0.0	10.883036235798386	0.0
19	0.0	0.0	0.0	11.237225684334419	0.0
20	0.0	0.0	0.0	11.653777069252492	0.0
21	0.0	0.0	0.0	12.034536216035802	0.0
22	0.0	0.0	0.0	12.48007272631454	0.0
23	0.0	0.0	0.0	12.932196765084342	0.0
24	0.0	0.0	0.0	13.239834345616877	0.0
25	0.0	0.0	0.0	13.509044676618226	0.0
26	0.0	0.0	0.0	13.762225354957993	0.0
27	0.0	0.0	0.0	14.056687878508408	0.0
28	0.0	0.0	0.0	14.373547998928428	0.0
29	0.0	0.0	0.0	14.66910844389402	0.0
30	0.0	0.0	0.0	15.06216431052731	0.0
31	0.0	0.0	0.0	15.33884050715186	0.0
32	0.0	0.0	0.0	15.61507753521034	0.0
33	0.0	0.0	0.0	15.91305340728932	0.0
34	0.0	0.0	0.0	16.214762212179902	0.0
35	0.0	0.0	0.0	16.546993232412397	0.0
36	0.0	0.0	0.0	16.836624901736034	0.0
37	0.0	0.0	0.0	17.145360403683746	0.0
38	0.0	0.0	0.0	17.458707175575203	0.0
39	0.0	0.0	0.0	17.752071777710437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGGT	30	2.1626038E-6	45.000004	22
CACGCTT	30	2.1626038E-6	45.000004	27
CACGACC	30	2.1626038E-6	45.000004	27
GCACCTA	60	0.0	45.000004	9
CCCACGG	30	2.1626038E-6	45.000004	2
ATACTAC	30	2.1626038E-6	45.000004	40
TAGTTCG	60	0.0	45.000004	1
TACGACG	30	2.1626038E-6	45.000004	1
CGCTACT	30	2.1626038E-6	45.000004	29
AACGCCA	30	2.1626038E-6	45.000004	14
TAGGGCG	30	2.1626038E-6	45.000004	5
AGTTCGG	60	0.0	45.000004	2
GAGCCGT	30	2.1626038E-6	45.000004	8
CCAAGCG	30	2.1626038E-6	45.000004	1
CGCACCC	30	2.1626038E-6	45.000004	31
ACATCGG	105	0.0	45.000004	2
CGGTATC	30	2.1626038E-6	45.000004	27
ATTGCCG	30	2.1626038E-6	45.000004	12
CGCCGAC	30	2.1626038E-6	45.000004	35
TGTCGCC	30	2.1626038E-6	45.000004	24
>>END_MODULE