FastQCFastQC Report
Sat 18 Jun 2016
SRR3548600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355956
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120783.3931160031015066No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26970.757677915247952No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21310.5986694984773399No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT14610.4104439874591242No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14270.40089224510894605No Hit
TAAGTAGGGATTTATATGGATTCCTTTTATTTTTAGTTCAGAAAGAAAGAA4230.1188349121801571No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC3740.10506916585195922No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT3620.10169796266954344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT650.045.00000410
CGAGGCT650.045.0000044
TCCGGAC207.0267176E-445.018
TCACGCC253.885363E-545.043
CTAGACT207.0267176E-445.041
CTATGCG253.885363E-545.01
TCGTTGA253.885363E-545.024
TTGGCGG207.0267176E-445.02
TCGTTAG207.0267176E-445.016
GTACCCG700.045.01
CAAACGA207.0267176E-445.017
ACGCCTC253.885363E-545.045
GCGACTC207.0267176E-445.028
GTCGATG207.0267176E-445.09
GGTCGAT207.0267176E-445.08
TGCCACG253.885363E-545.01
TAGACTT406.7921064E-945.012
ACGTTAA253.885363E-545.022
TAGACGG406.7921064E-945.02
GCAACGC207.0267176E-445.043