##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355956
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62407432379283	31.0	31.0	34.0	30.0	34.0
2	31.820039555450673	33.0	31.0	34.0	30.0	34.0
3	31.9979295193788	33.0	31.0	34.0	30.0	34.0
4	35.727598354852844	37.0	35.0	37.0	33.0	37.0
5	35.469583319286656	37.0	35.0	37.0	33.0	37.0
6	34.00158446549573	37.0	35.0	37.0	32.0	37.0
7	34.894686421917314	37.0	35.0	37.0	32.0	37.0
8	35.62606052433447	37.0	35.0	37.0	32.0	37.0
9	37.342399060558044	39.0	37.0	39.0	34.0	39.0
10	36.754562361640204	38.0	37.0	39.0	32.0	39.0
11	36.780231264538315	39.0	37.0	39.0	32.0	39.0
12	36.783082740563444	39.0	37.0	39.0	32.0	39.0
13	36.77007551495129	39.0	37.0	39.0	32.0	39.0
14	37.96629077751183	40.0	37.0	41.0	33.0	41.0
15	37.92480250368023	40.0	37.0	41.0	33.0	41.0
16	37.83805301778872	40.0	37.0	41.0	33.0	41.0
17	37.78940936520244	39.0	37.0	41.0	33.0	41.0
18	37.60205755767567	39.0	37.0	41.0	33.0	41.0
19	37.48122520760993	39.0	36.0	41.0	32.0	41.0
20	37.38364292215892	39.0	35.0	40.0	32.0	41.0
21	37.353664497859285	39.0	35.0	40.0	32.0	41.0
22	37.33210284417175	39.0	35.0	40.0	32.0	41.0
23	37.1540639854364	39.0	35.0	40.0	32.0	41.0
24	36.99937351807527	39.0	35.0	40.0	31.0	41.0
25	37.08258324062525	39.0	35.0	40.0	32.0	41.0
26	36.97982615828923	39.0	35.0	40.0	31.0	41.0
27	36.94538650844486	39.0	35.0	40.0	31.0	41.0
28	36.91264931620762	39.0	35.0	40.0	31.0	41.0
29	36.709110114733285	39.0	35.0	40.0	31.0	41.0
30	36.75123329849757	39.0	35.0	40.0	31.0	41.0
31	36.62867039746485	39.0	35.0	40.0	31.0	41.0
32	36.54261762689771	39.0	35.0	40.0	31.0	41.0
33	36.472670779534546	39.0	35.0	40.0	30.0	41.0
34	36.3629605906348	38.0	35.0	40.0	30.0	41.0
35	36.236282012383555	38.0	35.0	40.0	30.0	41.0
36	36.14168605108497	38.0	35.0	40.0	30.0	41.0
37	36.118851768196066	38.0	35.0	40.0	30.0	41.0
38	35.98787209655126	38.0	35.0	40.0	30.0	41.0
39	35.91925406510917	38.0	35.0	40.0	29.0	41.0
40	35.7997505309645	38.0	35.0	40.0	29.0	41.0
41	35.670838530604904	38.0	35.0	40.0	28.0	41.0
42	35.512167234152535	38.0	35.0	40.0	27.0	41.0
43	35.40864320309252	38.0	34.0	40.0	27.0	41.0
44	35.3258633089483	38.0	34.0	40.0	27.0	41.0
45	35.235737001202395	38.0	34.0	40.0	26.0	41.0
46	35.041985526301005	38.0	34.0	40.0	26.0	41.0
47	34.97417377428671	38.0	34.0	40.0	26.0	41.0
48	34.82135151535583	37.0	34.0	40.0	25.0	41.0
49	34.64616132330962	37.0	33.0	40.0	24.0	41.0
50	34.51387531043163	37.0	33.0	40.0	24.0	41.0
51	33.434233444583036	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	6.0
14	11.0
15	15.0
16	50.0
17	83.0
18	199.0
19	386.0
20	682.0
21	1018.0
22	1438.0
23	1897.0
24	2314.0
25	2690.0
26	3148.0
27	3717.0
28	4556.0
29	5743.0
30	7329.0
31	9690.0
32	12468.0
33	16819.0
34	24132.0
35	36098.0
36	37107.0
37	48249.0
38	67294.0
39	68802.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.38582296688355	33.66989178437784	22.179145737113576	24.76513951162503
2	22.743541336569688	34.38964366382362	20.654238164267493	22.2125768353392
3	20.281439279012012	35.26081875287957	19.962298711076652	24.495443257031766
4	19.28075380103159	37.60886176943218	18.623931047657578	24.486453381878658
5	16.63716863882053	39.97572733708661	15.737619256312577	27.649484767780287
6	17.11559855712504	45.90426906696333	17.251289485217274	19.728842890694356
7	81.71768420816056	11.40562316690827	3.8510377687129873	3.025654856218184
8	82.92963175223905	11.173291080920114	2.9267662295339876	2.9703109373068584
9	76.56929508141455	14.72766296958051	5.595073548416096	3.107968400588837
10	28.67180213284788	48.24247940756723	9.803177920866624	13.28254053871827
11	18.252536830394767	33.8024924428862	30.91589971794267	17.029071008776366
12	22.773882165211432	30.933598534650354	30.59254514602928	15.699974154108935
13	24.374360876063335	32.147793547517104	25.705424265920506	17.772421310499052
14	19.20237332704042	33.9808852779557	27.82675386845565	18.989987526548223
15	18.347773320298014	37.23212981379721	26.55496746788929	17.865129398015487
16	21.24167031880345	34.54752834620009	26.34820033936779	17.86260099562867
17	19.349301599074042	34.86919731652227	26.69431053276248	19.087190551641214
18	19.82632684938588	33.873006776118395	27.28651855847352	19.014147816022206
19	18.774792390070683	38.05779365989055	26.346514737776577	16.820899212262187
20	20.24435604400544	36.32977109530391	24.709233725516636	18.71663913517401
21	20.75902639652092	35.337232691680995	27.020474440661207	16.88326647113688
22	19.02875636314601	33.06110867635326	26.058557799278564	21.851577161222174
23	18.501163065097934	36.03310521525132	25.668621964512468	19.797109755138276
24	16.868376990414546	36.44045893312657	27.58683657530706	19.104327501151825
25	19.21080133499646	36.16008720178899	25.10759756823877	19.521513894975783
26	20.707053680792008	37.315005225364935	23.74478868174718	18.233152412095876
27	17.528009079773906	35.69823236579802	25.501747406982883	21.27201114744519
28	18.019923810808077	35.95584847565429	26.455516974008024	19.568710739529603
29	18.180617829169897	37.65437301239479	25.12613918574206	19.038869972693252
30	21.626268415197384	34.54359527582061	24.99690973041612	18.83322657856589
31	19.234118823674837	37.348998190787626	23.00509051680545	20.41179246873209
32	21.096146714762497	35.18496668127521	24.300756273247256	19.418130330715034
33	19.77800627043792	33.990156086707344	26.416748137410238	19.815089505444494
34	18.729000213509536	36.17385294811719	24.774972187573745	20.32217465079954
35	20.060906404162314	34.837732753486385	25.501747406982883	19.599613435368415
36	20.75172212295902	36.43961613233096	24.632538853116678	18.176122891593344
37	21.240265650810773	36.08760633336704	22.896369214172537	19.775758801649644
38	20.52107563856207	35.31953387497332	25.155075346391126	19.00431514007349
39	20.414320871118903	34.36997831192619	24.535897695220758	20.679803121734146
40	22.351077099416784	33.922451089460495	24.61146883322658	19.115002977896147
41	20.95202777871422	32.590544898807714	26.44034655968715	20.017080762790908
42	20.802290170695255	35.011630650979335	24.461450291609076	19.724628886716335
43	18.93351987324276	34.62956095697221	26.00827068514086	20.428648484644167
44	21.01973277596107	33.33501893492454	24.863747204710695	20.78150108440369
45	21.950184854307835	33.71849329692434	25.32532110710313	19.0060007416647
46	19.65074335030172	34.0238681185315	26.73757430693681	19.58781422422996
47	20.10726044792053	34.44274011394667	25.508208879749183	19.94179055838362
48	19.570677274719348	33.38137297868276	27.092393441886074	19.95555630471182
49	20.87926597669375	32.29135061636831	25.82285451010799	21.006528896829945
50	19.86565755318073	31.564294463360643	26.51057995932081	22.059468024137814
51	20.734866107046937	31.06591825956017	27.372484239625123	20.82673139376777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13890.0
1	10326.5
2	6763.0
3	4378.0
4	1993.0
5	1521.5
6	1050.0
7	1151.0
8	1252.0
9	1457.0
10	1662.0
11	1897.5
12	2133.0
13	2314.0
14	2495.0
15	2344.0
16	2193.0
17	2286.0
18	2379.0
19	2243.5
20	2108.0
21	2224.5
22	2341.0
23	2589.5
24	2838.0
25	3072.0
26	4157.0
27	5008.0
28	5665.0
29	6322.0
30	6371.0
31	6420.0
32	8426.0
33	10432.0
34	11066.0
35	11700.0
36	13415.5
37	15131.0
38	15230.0
39	15329.0
40	15576.0
41	15823.0
42	17920.5
43	20018.0
44	22202.5
45	24387.0
46	26281.0
47	28175.0
48	29103.5
49	30032.0
50	29660.0
51	29288.0
52	25753.5
53	22219.0
54	19542.5
55	16866.0
56	15523.0
57	14180.0
58	12033.0
59	9886.0
60	9078.5
61	8271.0
62	7612.5
63	6954.0
64	5702.0
65	4450.0
66	3799.0
67	3148.0
68	2540.5
69	1933.0
70	1714.5
71	1496.0
72	1119.0
73	742.0
74	591.0
75	429.5
76	419.0
77	288.5
78	158.0
79	152.0
80	146.0
81	117.0
82	88.0
83	62.5
84	37.0
85	30.5
86	24.0
87	18.5
88	13.0
89	10.5
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	355956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.4117669454856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72482844540919	21.514761777778446
2	9.55927052984586	5.431915329256584
3	3.877637423636824	3.3051159233838794
4	2.0236802568321206	2.2998532731717867
5	1.2907926691475324	1.8336850245380496
6	0.8808584200808273	1.5016046485983072
7	0.6438965040573879	1.2805966187204
8	0.5019193245029376	1.1408331898570423
9	0.4109339489426913	1.0507823628613042
>10	3.98535941723862	24.770795149801334
>50	0.7438804331223106	14.864920109161844
>100	0.35194343072453405	15.38221523832374
>500	0.0	0.0
>1k	0.00399935716732425	2.1918996247189417
>5k	0.0	0.0
>10k+	9.998392918310626E-4	3.431021729828328
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12078	3.3931160031015066	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2697	0.757677915247952	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2131	0.5986694984773399	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	1461	0.4104439874591242	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1427	0.40089224510894605	No Hit
TAAGTAGGGATTTATATGGATTCCTTTTATTTTTAGTTCAGAAAGAAAGAA	423	0.1188349121801571	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	374	0.10506916585195922	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	362	0.10169796266954344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5183224892964299	0.0
2	0.0	0.0	0.0	0.665250761330052	0.0
3	0.0	0.0	0.0	0.8298778500713572	0.0
4	0.0	0.0	0.0	1.7100428142804167	0.0
5	0.0	0.0	0.0	1.8496668127521378	0.0
6	0.0	0.0	0.0	2.73179831215094	0.0
7	0.0	0.0	0.0	3.4184000269696253	0.0
8	0.0	0.0	0.0	4.038982346132668	0.0
9	0.0	0.0	0.0	4.942464799020104	0.0
10	0.0	0.0	0.0	6.22717414511906	0.0
11	0.0	0.0	0.0	7.393048579037859	0.0
12	0.0	0.0	0.0	7.971209924822169	0.0
13	0.0	0.0	0.0	8.18331479171583	0.0
14	0.0	0.0	0.0	8.410028205733292	0.0
15	0.0	0.0	0.0	8.61342413107238	0.0
16	0.0	0.0	0.0	9.061513220735147	0.0
17	0.0	0.0	0.0	9.705132094978032	0.0
18	0.0	0.0	0.0	10.488655901291171	0.0
19	0.0	0.0	0.0	10.808077402825068	0.0
20	0.0	0.0	0.0	11.173291080920114	0.0
21	0.0	0.0	0.0	11.524177145489892	0.0
22	0.0	0.0	0.0	11.973951836743867	0.0
23	0.0	0.0	0.0	12.415017586443268	0.0
24	0.0	0.0	0.0	12.739776826349324	0.0
25	0.0	0.0	0.0	13.003573475373361	0.0
26	0.0	0.0	0.0	13.259784917236962	0.0
27	0.0	0.0	0.0	13.548865590129116	0.0
28	0.0	0.0	0.0	13.82446145029161	0.0
29	0.0	0.0	0.0	14.136297744665072	0.0
30	0.0	0.0	0.0	14.512748766701502	0.0
31	0.0	0.0	0.0	14.80800997876142	0.0
32	0.0	0.0	0.0	15.076020631763477	0.0
33	0.0	0.0	0.0	15.362853835867355	0.0
34	0.0	0.0	0.0	15.657834114328738	0.0
35	0.0	0.0	0.0	15.991864162986436	0.0
36	0.0	0.0	0.0	16.280663902280057	0.0
37	0.0	0.0	0.0	16.56468777039859	0.0
38	0.0	0.0	0.0	16.873995662385237	0.0
39	0.0	0.0	0.0	17.188079425546977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGAT	65	0.0	45.000004	10
CGAGGCT	65	0.0	45.000004	4
TCCGGAC	20	7.0267176E-4	45.0	18
TCACGCC	25	3.885363E-5	45.0	43
CTAGACT	20	7.0267176E-4	45.0	41
CTATGCG	25	3.885363E-5	45.0	1
TCGTTGA	25	3.885363E-5	45.0	24
TTGGCGG	20	7.0267176E-4	45.0	2
TCGTTAG	20	7.0267176E-4	45.0	16
GTACCCG	70	0.0	45.0	1
CAAACGA	20	7.0267176E-4	45.0	17
ACGCCTC	25	3.885363E-5	45.0	45
GCGACTC	20	7.0267176E-4	45.0	28
GTCGATG	20	7.0267176E-4	45.0	9
GGTCGAT	20	7.0267176E-4	45.0	8
TGCCACG	25	3.885363E-5	45.0	1
TAGACTT	40	6.7921064E-9	45.0	12
ACGTTAA	25	3.885363E-5	45.0	22
TAGACGG	40	6.7921064E-9	45.0	2
GCAACGC	20	7.0267176E-4	45.0	43
>>END_MODULE