FastQCFastQC Report
Sat 18 Jun 2016
SRR3548599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350573
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118953.393016575720321No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27340.7798661049196601No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21730.6198423723447043No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13820.3942117618869679No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT13460.383942859261837No Hit
TAAGTAGGGATTTATATGGATTCCTTTTATTTTTAGTTCAGAAAGAAAGAA4140.11809238018900486No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC3520.10040704789016838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC207.0265704E-445.00000428
TTGGCGC207.0265704E-445.00000425
TCGCAGT207.0265704E-445.00000434
TACCCGT207.0265704E-445.00000424
ACGCCTA207.0265704E-445.00000432
CTACTTA207.0265704E-445.00000412
GCGACCG207.0265704E-445.00000432
TTGGACG207.0265704E-445.0000041
CGAAATC207.0265704E-445.00000435
CCGATTA207.0265704E-445.00000412
CCTAGGA207.0265704E-445.0000043
CTATCCT207.0265704E-445.00000419
TAGACGG207.0265704E-445.0000042
ATCAGTT207.0265704E-445.00000423
TAGCGAT207.0265704E-445.00000445
CAACCGT207.0265704E-445.00000444
ATTCGGA207.0265704E-445.00000443
CACGATT207.0265704E-445.00000436
TTGTACG207.0265704E-445.0000041
TTCAACG207.0265704E-445.0000041