##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350573
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60938520650478	31.0	31.0	34.0	30.0	34.0
2	31.815419327786223	31.0	31.0	34.0	30.0	34.0
3	31.98903509397472	33.0	31.0	34.0	30.0	34.0
4	35.735510150524995	37.0	35.0	37.0	35.0	37.0
5	35.498526697720585	37.0	35.0	37.0	33.0	37.0
6	32.46154153343241	37.0	35.0	37.0	25.0	37.0
7	34.12582828683327	37.0	35.0	37.0	25.0	37.0
8	35.30854058926386	37.0	35.0	37.0	32.0	37.0
9	37.20441391664504	39.0	37.0	39.0	34.0	39.0
10	36.71260479272505	38.0	37.0	39.0	32.0	39.0
11	36.78033391048369	39.0	37.0	39.0	32.0	39.0
12	36.78290398861293	39.0	37.0	39.0	32.0	39.0
13	36.81105219169759	39.0	37.0	39.0	33.0	39.0
14	37.978095860206004	40.0	37.0	41.0	33.0	41.0
15	37.973731576590325	40.0	37.0	41.0	33.0	41.0
16	37.913008132400385	40.0	37.0	41.0	33.0	41.0
17	37.892002521586086	39.0	37.0	41.0	33.0	41.0
18	37.69513910084348	39.0	37.0	41.0	33.0	41.0
19	37.5930234216554	39.0	36.0	41.0	33.0	41.0
20	37.47837397631877	39.0	36.0	41.0	32.0	41.0
21	37.44163697717737	39.0	36.0	40.0	32.0	41.0
22	37.3649539468242	39.0	35.0	40.0	32.0	41.0
23	37.205409429705085	39.0	35.0	40.0	32.0	41.0
24	37.121803447498806	39.0	35.0	40.0	32.0	41.0
25	37.1592107777838	39.0	35.0	40.0	32.0	41.0
26	37.05284206142515	39.0	35.0	40.0	32.0	41.0
27	36.991337039646524	39.0	35.0	40.0	31.0	41.0
28	36.97463010557002	39.0	35.0	40.0	31.0	41.0
29	36.746166989471526	39.0	35.0	40.0	31.0	41.0
30	36.799565283122206	39.0	35.0	40.0	31.0	41.0
31	36.34676657928591	39.0	35.0	40.0	30.0	41.0
32	36.464696368516684	39.0	35.0	40.0	30.0	41.0
33	36.44623231110211	39.0	35.0	40.0	30.0	41.0
34	36.37278113260291	38.0	35.0	40.0	30.0	41.0
35	36.357862699066956	38.0	35.0	40.0	30.0	41.0
36	36.22961836764383	38.0	35.0	40.0	30.0	41.0
37	36.18278646672733	38.0	35.0	40.0	30.0	41.0
38	36.04913099411535	38.0	35.0	40.0	30.0	41.0
39	35.950070313458255	38.0	35.0	40.0	30.0	41.0
40	35.84407527105624	38.0	35.0	40.0	29.0	41.0
41	35.78668066280061	38.0	35.0	40.0	29.0	41.0
42	35.62422662327104	38.0	35.0	40.0	28.0	41.0
43	35.528982551422956	38.0	35.0	40.0	28.0	41.0
44	35.40374757896358	38.0	34.0	40.0	27.0	41.0
45	35.30853773679091	38.0	34.0	40.0	27.0	41.0
46	35.01856104149493	38.0	34.0	40.0	26.0	41.0
47	35.02673337650076	38.0	34.0	40.0	26.0	41.0
48	34.98758318524244	38.0	34.0	40.0	26.0	41.0
49	34.87942026339735	37.0	34.0	40.0	26.0	41.0
50	34.730698028655944	37.0	33.0	40.0	25.0	41.0
51	33.68772837611567	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	3.0
14	9.0
15	29.0
16	44.0
17	99.0
18	199.0
19	399.0
20	661.0
21	987.0
22	1417.0
23	1786.0
24	2293.0
25	2632.0
26	2951.0
27	3471.0
28	4289.0
29	5493.0
30	7225.0
31	9341.0
32	12592.0
33	16591.0
34	24267.0
35	35922.0
36	37421.0
37	49069.0
38	65954.0
39	65411.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.30068773122859	33.53937696285795	22.199655991762057	24.960279314151403
2	22.72422576752916	34.51378172306481	20.609402321342486	22.15259018806354
3	20.226029956670935	35.34556283570041	19.93365147914985	24.494755728478804
4	19.311812375739144	37.51144554771759	18.602117105424547	24.574624971118713
5	16.678409346983365	40.00992660587096	15.800418172534677	27.511245874610996
6	16.338394571173477	48.31689833501154	16.448785274393636	18.895921819421346
7	81.77669130252472	11.462947802597462	3.7729659728501628	2.987394922027652
8	82.83923747693063	11.302353575432221	2.8133940719907122	3.0450148756464417
9	76.67789590185211	14.747570406163623	5.4661939168161835	3.108339775168082
10	28.758917543564394	48.30292121754955	9.756313235759743	13.181848003126309
11	18.303463187410326	33.724787704700596	31.054302527576283	16.917446580312802
12	22.66974353415694	30.78959303768402	30.73996000832922	15.80070341982982
13	24.29622361105961	32.15820955977785	25.739004429890493	17.80656239927205
14	19.239359562772947	34.066513964281334	27.72917480809988	18.964951664845838
15	18.306600907656893	37.26584762659988	26.580198703265793	17.84735276247743
16	21.340205891497636	34.5425916998742	26.387942026339733	17.729260382288427
17	19.471836108314104	34.82555701665559	26.74221916690675	18.96038770812356
18	19.80842791658228	33.891372125063825	27.42766841713423	18.87253154121966
19	18.940135150168437	38.05455639766896	26.278121817709867	16.72718663445274
20	20.09453095361023	36.37131210903293	24.8421869339624	18.69197000339444
21	20.801088503678265	35.34527758840527	27.112470156001745	16.741163751914723
22	19.045961896666313	33.26981826894826	25.942385751327112	21.741834083058308
23	18.31629931569174	36.14054704726262	25.69222387348712	19.85092976355852
24	16.85497742267659	36.48683726356565	27.66328268292196	18.994902630835803
25	19.357737190257094	36.14168803644319	25.018755009655617	19.481819763644094
26	20.553493851494554	37.56706877027038	23.66240412125292	18.21703325698214
27	17.347599501387727	35.73492539356995	25.586967621579525	21.33050748346279
28	18.038753697518064	36.277465748931036	26.399351918145435	19.284428635405465
29	18.327423960202296	37.69343332201852	24.944875960213707	19.034266757565472
30	21.625167939345015	34.67123822998349	24.939741508901143	18.763852321770358
31	19.32664523508656	37.34885458948636	23.097614476870724	20.226885698556362
32	21.067509477341382	35.36182193152354	24.200665767186862	19.37000282394822
33	19.644695969170474	34.071077921003614	26.446702969139096	19.83752314068682
34	18.652035382074487	36.41010574117231	24.538969059225895	20.398889817527305
35	19.972730358584375	34.72201224851885	25.71704038816452	19.588217004732254
36	20.55092662583827	36.65313643663374	24.606857915469817	18.189079022058174
37	21.262618627218867	36.06638275052557	22.887387220350682	19.78361140190488
38	20.537805250261716	35.35896945857211	25.265779167249047	18.83744612391713
39	20.248564492987196	34.56797870914189	24.54724123078503	20.636215567085888
40	22.316892630065635	34.04854338468735	24.58346763726813	19.05109634797888
41	20.904633271815	32.83196367090449	26.29323992435242	19.970163132928093
42	20.672156726273844	35.253713206664514	24.362400983532673	19.711729083528965
43	18.860836402118817	34.78419615885993	25.931546354111696	20.423421084909563
44	20.90919722853728	33.537950726382235	24.97340068972796	20.579451355352525
45	21.92867106137666	33.84402107407016	25.278900542825607	18.948407321727572
46	19.716007792956102	34.14723894880667	26.648943301395146	19.487809956842085
47	20.108793318367358	34.55571307545076	25.56243635419727	19.773057251984607
48	19.467842646182106	33.61781996902214	26.993522033927313	19.920815350868434
49	20.92403008788469	32.38983036343358	25.89389371115288	20.792245837528846
50	19.98328450850465	31.89264432800016	26.341731964526648	21.782339198968543
51	20.770281795802873	31.206054088592104	27.38858953769971	20.635074577905314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13712.0
1	10295.0
2	6878.0
3	4437.5
4	1997.0
5	1520.5
6	1044.0
7	1158.5
8	1273.0
9	1458.5
10	1644.0
11	1877.0
12	2110.0
13	2356.5
14	2603.0
15	2381.0
16	2159.0
17	2254.0
18	2349.0
19	2231.0
20	2113.0
21	2189.5
22	2266.0
23	2600.5
24	2935.0
25	3073.5
26	4118.0
27	5024.0
28	5695.0
29	6366.0
30	6314.0
31	6262.0
32	8269.5
33	10277.0
34	11025.5
35	11774.0
36	13378.0
37	14982.0
38	14976.0
39	14970.0
40	15204.5
41	15439.0
42	17739.0
43	20039.0
44	22032.0
45	24025.0
46	25710.0
47	27395.0
48	28429.5
49	29464.0
50	29001.5
51	28539.0
52	25146.0
53	21753.0
54	19259.0
55	16765.0
56	15241.0
57	13717.0
58	11745.5
59	9774.0
60	8929.0
61	8084.0
62	7461.0
63	6838.0
64	5672.5
65	4507.0
66	3752.0
67	2997.0
68	2446.0
69	1895.0
70	1647.5
71	1400.0
72	1040.5
73	681.0
74	540.0
75	422.0
76	445.0
77	304.0
78	163.0
79	143.0
80	123.0
81	106.5
82	90.0
83	65.5
84	41.0
85	32.5
86	24.0
87	17.0
88	10.0
89	9.0
90	8.0
91	5.5
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.923352146716745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58150242070796	21.860704102920604
2	9.575433749605821	5.539072845948111
3	3.6725154019055997	3.1866436870066988
4	2.0253907436251923	2.3432435885028773
5	1.4025647523776945	2.0283437120796317
6	0.932710059044166	1.618626089311163
7	0.7247746756662172	1.467403921992149
8	0.5528281309819921	1.2791714167202726
9	0.40887288526216875	1.0643376999313638
>10	4.08173102815634	25.227203380353743
>50	0.7027815118280232	14.274442697612386
>100	0.3338961948087621	14.46383177488787
>500	0.0	0.0
>1k	0.003998756824057031	2.2076116106844625
>5k	0.0	0.0
>10k+	9.996892060142577E-4	3.4393634720486816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11895	3.393016575720321	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2734	0.7798661049196601	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2173	0.6198423723447043	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1382	0.3942117618869679	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	1346	0.383942859261837	No Hit
TAAGTAGGGATTTATATGGATTCCTTTTATTTTTAGTTCAGAAAGAAAGAA	414	0.11809238018900486	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	352	0.10040704789016838	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4635268546066012	0.0
2	0.0	0.0	0.0	0.5918881374207369	0.0
3	0.0	0.0	0.0	0.7570463213082582	0.0
4	0.0	0.0	0.0	1.5506042963947595	0.0
5	0.0	0.0	0.0	1.6601392577294887	0.0
6	0.0	0.0	0.0	2.4922056176602307	0.0
7	0.0	0.0	0.0	3.126880849352346	0.0
8	0.0	0.0	0.0	3.7390215447282023	0.0
9	0.0	0.0	0.0	4.640973491968863	0.0
10	0.0	0.0	0.0	5.894350106825112	0.0
11	0.0	0.0	0.0	7.03904750223206	0.0
12	0.0	0.0	0.0	7.624089704569377	0.0
13	0.0	0.0	0.0	7.862271196013384	0.0
14	0.0	0.0	0.0	8.085905075405122	0.0
15	0.0	0.0	0.0	8.296417579220305	0.0
16	0.0	0.0	0.0	8.739977123166929	0.0
17	0.0	0.0	0.0	9.369803150841623	0.0
18	0.0	0.0	0.0	10.107737903375332	0.0
19	0.0	0.0	0.0	10.42065418614668	0.0
20	0.0	0.0	0.0	10.788908444175679	0.0
21	0.0	0.0	0.0	11.147464294169831	0.0
22	0.0	0.0	0.0	11.57904345172047	0.0
23	0.0	0.0	0.0	12.004632416073115	0.0
24	0.0	0.0	0.0	12.303001086792195	0.0
25	0.0	0.0	0.0	12.564287609142747	0.0
26	0.0	0.0	0.0	12.810170777555602	0.0
27	0.0	0.0	0.0	13.090854115975846	0.0
28	0.0	0.0	0.0	13.38522932456293	0.0
29	0.0	0.0	0.0	13.681030769625727	0.0
30	0.0	0.0	0.0	14.058412941099286	0.0
31	0.0	0.0	0.0	14.365909525262927	0.0
32	0.0	0.0	0.0	14.644881379912315	0.0
33	0.0	0.0	0.0	14.958082909978806	0.0
34	0.0	0.0	0.0	15.248464656433896	0.0
35	0.0	0.0	0.0	15.579351518799223	0.0
36	0.0	0.0	0.0	15.876008705747449	0.0
37	0.0	0.0	0.0	16.170383914334533	0.0
38	0.0	0.0	0.0	16.47759525120303	0.0
39	0.0	0.0	0.0	16.78737381372781	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTC	20	7.0265704E-4	45.000004	28
TTGGCGC	20	7.0265704E-4	45.000004	25
TCGCAGT	20	7.0265704E-4	45.000004	34
TACCCGT	20	7.0265704E-4	45.000004	24
ACGCCTA	20	7.0265704E-4	45.000004	32
CTACTTA	20	7.0265704E-4	45.000004	12
GCGACCG	20	7.0265704E-4	45.000004	32
TTGGACG	20	7.0265704E-4	45.000004	1
CGAAATC	20	7.0265704E-4	45.000004	35
CCGATTA	20	7.0265704E-4	45.000004	12
CCTAGGA	20	7.0265704E-4	45.000004	3
CTATCCT	20	7.0265704E-4	45.000004	19
TAGACGG	20	7.0265704E-4	45.000004	2
ATCAGTT	20	7.0265704E-4	45.000004	23
TAGCGAT	20	7.0265704E-4	45.000004	45
CAACCGT	20	7.0265704E-4	45.000004	44
ATTCGGA	20	7.0265704E-4	45.000004	43
CACGATT	20	7.0265704E-4	45.000004	36
TTGTACG	20	7.0265704E-4	45.000004	1
TTCAACG	20	7.0265704E-4	45.000004	1
>>END_MODULE