FastQCFastQC Report
Sat 18 Jun 2016
SRR3548598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519401
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234694.518474165432874No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58621.128607761633112No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45730.8804372729355546No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28200.5429331094857345No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT24460.4709270871638676RNA PCR Primer, Index 27 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT9060.17443170113265088No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT8890.1711587001180206No Hit
AGACACGGGGATGAGGTATGGGGGACTTTTGGTATAGCATTGAAAATGTAA8510.16384258020296455No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5770.11108950502598185No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5660.10897168084004459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGA351.21021E-745.00000432
ACTGCCG351.21021E-745.0000041
CACGGTT351.21021E-745.00000425
GGCAACG351.21021E-745.0000041
CGCGCCT351.21021E-745.00000417
TAGCCGT351.21021E-745.00000444
TACGCAG700.045.0000041
CGTAGTG351.21021E-745.00000428
CGAGGAC351.21021E-745.0000043
TTGCGCG351.21021E-745.00000414
ACGTACG351.21021E-745.0000041
GCCCGCC351.21021E-745.0000043
TCACGAG551.8189894E-1245.01
TCGCCTG253.8878432E-545.043
CTTCGTT207.029711E-445.035
CTAGATT253.8878432E-545.039
CGGGTAT406.8030204E-945.06
GACAGGT253.8878432E-545.039
CAGCGCG453.8380676E-1045.01
AGCCGAG253.8878432E-545.01