Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548598_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 519401 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 23469 | 4.518474165432874 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 5862 | 1.128607761633112 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4573 | 0.8804372729355546 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2820 | 0.5429331094857345 | No Hit |
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT | 2446 | 0.4709270871638676 | RNA PCR Primer, Index 27 (96% over 25bp) |
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT | 906 | 0.17443170113265088 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT | 889 | 0.1711587001180206 | No Hit |
AGACACGGGGATGAGGTATGGGGGACTTTTGGTATAGCATTGAAAATGTAA | 851 | 0.16384258020296455 | No Hit |
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 577 | 0.11108950502598185 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 566 | 0.10897168084004459 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCAGA | 35 | 1.21021E-7 | 45.000004 | 32 |
ACTGCCG | 35 | 1.21021E-7 | 45.000004 | 1 |
CACGGTT | 35 | 1.21021E-7 | 45.000004 | 25 |
GGCAACG | 35 | 1.21021E-7 | 45.000004 | 1 |
CGCGCCT | 35 | 1.21021E-7 | 45.000004 | 17 |
TAGCCGT | 35 | 1.21021E-7 | 45.000004 | 44 |
TACGCAG | 70 | 0.0 | 45.000004 | 1 |
CGTAGTG | 35 | 1.21021E-7 | 45.000004 | 28 |
CGAGGAC | 35 | 1.21021E-7 | 45.000004 | 3 |
TTGCGCG | 35 | 1.21021E-7 | 45.000004 | 14 |
ACGTACG | 35 | 1.21021E-7 | 45.000004 | 1 |
GCCCGCC | 35 | 1.21021E-7 | 45.000004 | 3 |
TCACGAG | 55 | 1.8189894E-12 | 45.0 | 1 |
TCGCCTG | 25 | 3.8878432E-5 | 45.0 | 43 |
CTTCGTT | 20 | 7.029711E-4 | 45.0 | 35 |
CTAGATT | 25 | 3.8878432E-5 | 45.0 | 39 |
CGGGTAT | 40 | 6.8030204E-9 | 45.0 | 6 |
GACAGGT | 25 | 3.8878432E-5 | 45.0 | 39 |
CAGCGCG | 45 | 3.8380676E-10 | 45.0 | 1 |
AGCCGAG | 25 | 3.8878432E-5 | 45.0 | 1 |