##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519401
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45277155800624	31.0	31.0	34.0	30.0	34.0
2	31.652336056341824	31.0	31.0	34.0	30.0	34.0
3	31.847019932576178	33.0	31.0	34.0	30.0	34.0
4	35.600366576113636	37.0	35.0	37.0	33.0	37.0
5	35.333139520332075	37.0	35.0	37.0	33.0	37.0
6	33.96792266476191	37.0	35.0	37.0	31.0	37.0
7	34.82002730067905	37.0	35.0	37.0	32.0	37.0
8	35.50296784180238	37.0	35.0	37.0	32.0	37.0
9	37.17614136283912	39.0	37.0	39.0	34.0	39.0
10	36.50962551092508	38.0	35.0	39.0	32.0	39.0
11	36.515052916725224	38.0	35.0	39.0	32.0	39.0
12	36.61559758259995	39.0	35.0	39.0	32.0	39.0
13	36.61806966101336	39.0	35.0	39.0	32.0	39.0
14	37.71443451206294	39.0	37.0	41.0	33.0	41.0
15	37.693346759055146	39.0	37.0	41.0	32.0	41.0
16	37.591893354075175	39.0	36.0	41.0	32.0	41.0
17	37.518383676581294	39.0	36.0	40.0	32.0	41.0
18	37.29630478185448	39.0	36.0	40.0	32.0	41.0
19	37.146152972366245	39.0	35.0	40.0	32.0	41.0
20	36.998482867764984	39.0	35.0	40.0	31.0	41.0
21	36.9729842645663	39.0	35.0	40.0	31.0	41.0
22	36.96448216310712	39.0	35.0	40.0	31.0	41.0
23	36.73218572933052	39.0	35.0	40.0	31.0	41.0
24	36.54358578439395	38.0	35.0	40.0	31.0	41.0
25	36.64855285222785	38.0	35.0	40.0	31.0	41.0
26	36.533581953057464	38.0	35.0	40.0	31.0	41.0
27	36.51783689288238	38.0	35.0	40.0	31.0	41.0
28	36.44239229420044	38.0	35.0	40.0	31.0	41.0
29	36.28060207816311	38.0	35.0	40.0	30.0	41.0
30	36.219044630256775	38.0	35.0	40.0	30.0	41.0
31	36.12755462542429	38.0	35.0	40.0	30.0	41.0
32	36.00799382365456	38.0	35.0	40.0	30.0	41.0
33	35.90095898929729	38.0	35.0	40.0	30.0	41.0
34	35.85791132477604	38.0	35.0	40.0	29.0	41.0
35	35.66880695262427	38.0	35.0	40.0	28.0	41.0
36	35.65060136580407	38.0	35.0	40.0	29.0	41.0
37	35.609788968446345	38.0	35.0	40.0	28.0	41.0
38	35.494419533270055	38.0	34.0	40.0	27.0	41.0
39	35.40797572588424	38.0	34.0	40.0	27.0	41.0
40	35.239652984880664	38.0	34.0	40.0	26.0	41.0
41	35.13834397700428	38.0	34.0	40.0	26.0	41.0
42	35.05049470447689	38.0	34.0	40.0	25.0	41.0
43	34.97270894742213	38.0	34.0	40.0	25.0	41.0
44	34.817239473932474	38.0	34.0	40.0	24.0	41.0
45	34.637607551775986	37.0	33.0	40.0	24.0	41.0
46	34.44431181303078	37.0	33.0	40.0	23.0	41.0
47	34.350249614459734	37.0	33.0	40.0	23.0	41.0
48	34.22600842123908	37.0	33.0	40.0	23.0	41.0
49	34.062749205334605	37.0	33.0	40.0	23.0	41.0
50	33.958065155823725	37.0	33.0	40.0	22.0	41.0
51	32.91661356062079	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	8.0
12	10.0
13	17.0
14	30.0
15	63.0
16	134.0
17	234.0
18	475.0
19	865.0
20	1358.0
21	2037.0
22	2766.0
23	3565.0
24	4136.0
25	4967.0
26	5640.0
27	6593.0
28	7926.0
29	9572.0
30	12197.0
31	15483.0
32	19743.0
33	26145.0
34	36941.0
35	57614.0
36	54151.0
37	68901.0
38	92810.0
39	85003.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.482059141203038	36.30682266687973	19.413901783015437	25.79721640890179
2	21.1237560189526	37.170124816856344	18.417561768267674	23.28855739592338
3	18.777206820934115	38.62391485576654	19.04405266836221	23.55482565493713
4	18.3557598079326	39.303736419452406	17.60739775241095	24.733106020204044
5	16.0777511017499	41.166459055719955	16.211366554935395	26.54442328759475
6	16.14725424094293	47.154125617778945	16.271628279498884	20.426991861779243
7	77.55221880589372	14.437785063948663	4.431450844337997	3.5785452858196267
8	79.4565278079942	14.031740408663056	3.3459696843094258	3.16576209903331
9	72.96597426651084	17.622800110126857	6.374265740728262	3.0369598826340343
10	28.02613009986504	50.24691904713314	10.024431989926857	11.702518863074966
11	16.5018935273517	35.49935406362329	32.43486246657207	15.56388994245294
12	19.813400436271785	32.04364258058802	30.84341385557594	17.299543127564252
13	21.735614679217022	32.67282889328284	27.4173519111438	18.17420451635634
14	16.54001436269857	36.338590029668794	28.314154189152507	18.80724141848013
15	14.732355155265392	40.157027036913675	27.35632006869452	17.754297739126418
16	17.648214000358106	37.884216626460095	26.94488458820834	17.52268478497346
17	17.442014936436394	36.67744189941875	27.756011251422315	18.124531912722542
18	17.21521521906966	36.295270898592804	27.570605370417077	18.918908511920463
19	16.94394119379824	39.48798712362895	26.426787780539506	17.1412839020333
20	18.349598864846236	39.03631298360997	25.75293463046856	16.861153521075238
21	18.532501862722636	37.78294612447801	26.47241726527288	17.212134747526477
22	17.16477249755006	35.62854133896546	26.622975311945872	20.5837108515386
23	17.367313501514246	37.95988070873949	25.72482532763703	18.947980462109236
24	15.005747004722746	38.0490218540203	27.94719301657101	18.998038124685937
25	17.138203430490123	39.431768517965885	23.84073192003866	19.58929613150533
26	18.279518137238856	39.759068619428916	22.615666893209678	19.345746350122546
27	15.835934085610154	37.88729709800327	24.007654971784806	22.269113844601762
28	16.979751675487726	37.654528967021626	26.14184416279522	19.223875194695427
29	18.07813231010337	37.27293555461002	24.304150357816024	20.344781777470587
30	18.883483089173875	36.90712955885722	24.717511133016686	19.491876218952218
31	18.460303310929323	36.76446522051363	23.382896836933316	21.392334631623736
32	18.012479760339314	36.103319015558306	25.660327954701668	20.223873269400713
33	18.04155171052809	37.64586514080643	23.44007808995362	20.872505058711862
34	17.21194221805503	35.93177525649739	25.425827058476973	21.430455466970606
35	17.040013400051212	35.612368863363756	25.8536275440363	21.493990192548722
36	17.43623905229293	37.046905955129084	26.03999607239878	19.476858920179208
37	16.974168320815707	36.41560181824833	25.091403366570336	21.518826494365626
38	17.33631625661098	36.08445112735632	26.902143045546694	19.677089570486004
39	17.4578023530952	35.226539802580284	26.87307109535792	20.442586748966598
40	18.737353220344204	35.48010111647841	27.0925546928096	18.68999097036779
41	17.395615333817226	33.545757516831884	29.3618995727771	19.69672757657378
42	18.66630984537958	35.1658930190739	25.59159493339443	20.576202202152093
43	16.51864359136775	34.719609704255475	26.886548158359343	21.875198546017433
44	19.30666286741843	33.04652859736505	25.063294063738805	22.583514471477724
45	19.57408630326087	34.07463597490186	26.458362613857116	19.892915107980155
46	17.278749944647778	35.66030870175452	26.993594544484896	20.067346809112806
47	17.643015704628986	34.35880947476035	26.987241071927087	21.01093374868358
48	17.67228018428921	32.75850450807757	29.944108694438405	19.625106613194816
49	17.560420561377434	32.774869513150726	27.944305074499283	21.720404850972564
50	17.023455865506612	32.59408433946026	26.9273644063065	23.45509538872663
51	18.147250390353502	31.000132845335298	29.36883063374926	21.483786130561935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26998.0
1	20548.0
2	14098.0
3	8617.0
4	3136.0
5	2523.5
6	1911.0
7	2074.5
8	2238.0
9	2605.5
10	2973.0
11	3389.5
12	3806.0
13	4122.0
14	4438.0
15	4684.5
16	4931.0
17	4643.0
18	4355.0
19	4310.0
20	4265.0
21	4371.5
22	4478.0
23	4544.5
24	4611.0
25	4750.0
26	5632.0
27	6375.0
28	7173.0
29	7971.0
30	9642.0
31	11313.0
32	13580.0
33	15847.0
34	16086.0
35	16325.0
36	18742.0
37	21159.0
38	21891.5
39	22624.0
40	23635.0
41	24646.0
42	26517.5
43	28389.0
44	31867.5
45	35346.0
46	37768.0
47	40190.0
48	42334.0
49	44478.0
50	43109.0
51	41740.0
52	36715.5
53	31691.0
54	27201.5
55	22712.0
56	19418.5
57	16125.0
58	14854.5
59	13584.0
60	11645.0
61	9706.0
62	8616.5
63	7527.0
64	6156.5
65	4786.0
66	4295.5
67	3805.0
68	2970.0
69	2135.0
70	1909.0
71	1683.0
72	1312.0
73	941.0
74	668.5
75	338.0
76	280.0
77	246.0
78	212.0
79	197.0
80	182.0
81	116.0
82	50.0
83	37.0
84	24.0
85	21.5
86	19.0
87	12.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.241290301733997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8823904214898	22.481402974060362
2	9.658265860329267	5.648403116664294
3	3.537893737013722	3.1035773346211437
4	1.8743350689113047	2.192319034910242
5	1.1716387324209832	1.7130114151738804
6	0.8575267888581628	1.5045113864709174
7	0.5946031724940076	1.2170874786860506
8	0.4863928294688698	1.1378203141744818
9	0.39794183516715964	1.0472699452794925
>10	3.493451152751951	21.980598966298547
>50	0.6915152177183187	14.084764321319232
>100	0.3474151237626069	15.56070803471501
>500	0.0033150298068951043	0.7345791054849081
>1k	0.0019890178841370626	1.9075016676869914
>5k	6.630059613790208E-4	1.1364747205997707
>10k+	6.630059613790208E-4	4.5499701838546605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23469	4.518474165432874	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5862	1.128607761633112	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4573	0.8804372729355546	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2820	0.5429331094857345	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	2446	0.4709270871638676	RNA PCR Primer, Index 27 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	906	0.17443170113265088	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	889	0.1711587001180206	No Hit
AGACACGGGGATGAGGTATGGGGGACTTTTGGTATAGCATTGAAAATGTAA	851	0.16384258020296455	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	577	0.11108950502598185	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	566	0.10897168084004459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9252947144884203E-4	0.0	0.0	0.5883700647476613	0.0
2	1.9252947144884203E-4	0.0	0.0	0.7912961276547408	0.0
3	1.9252947144884203E-4	0.0	0.0	1.0231016112791465	0.0
4	1.9252947144884203E-4	0.0	0.0	2.25625287590898	0.0
5	1.9252947144884203E-4	0.0	0.0	2.443969110571601	0.0
6	1.9252947144884203E-4	0.0	0.0	3.59799076243596	0.0
7	1.9252947144884203E-4	0.0	0.0	4.491905098372934	0.0
8	1.9252947144884203E-4	0.0	0.0	5.076232044220169	0.0
9	1.9252947144884203E-4	0.0	0.0	5.976114793772057	0.0
10	1.9252947144884203E-4	0.0	0.0	7.300139968925743	0.0
11	1.9252947144884203E-4	0.0	0.0	8.643418091224314	0.0
12	1.9252947144884203E-4	0.0	0.0	9.333058657954066	0.0
13	1.9252947144884203E-4	0.0	0.0	9.591048149695515	0.0
14	1.9252947144884203E-4	0.0	0.0	9.848460053022617	0.0
15	1.9252947144884203E-4	0.0	0.0	10.070831592546028	0.0
16	1.9252947144884203E-4	0.0	0.0	10.591431283343697	0.0
17	1.9252947144884203E-4	0.0	0.0	11.330166865292904	0.0
18	1.9252947144884203E-4	0.0	0.0	12.21695761078627	0.0
19	1.9252947144884203E-4	0.0	0.0	12.595855610597592	0.0
20	1.9252947144884203E-4	0.0	0.0	13.015377328884618	0.0
21	1.9252947144884203E-4	0.0	0.0	13.452996817487836	0.0
22	1.9252947144884203E-4	0.0	0.0	13.969938448327978	0.0
23	1.9252947144884203E-4	0.0	0.0	14.471862780395108	0.0
24	1.9252947144884203E-4	0.0	0.0	14.822073888960553	0.0
25	1.9252947144884203E-4	0.0	0.0	15.119916981291912	0.0
26	1.9252947144884203E-4	0.0	0.0	15.428541724024406	0.0
27	1.9252947144884203E-4	0.0	0.0	15.734278524685166	0.0
28	1.9252947144884203E-4	0.0	0.0	16.092383341580014	0.0
29	1.9252947144884203E-4	0.0	0.0	16.444712274331394	0.0
30	1.9252947144884203E-4	0.0	0.0	16.86750699363305	0.0
31	1.9252947144884203E-4	0.0	0.0	17.19538468351043	0.0
32	1.9252947144884203E-4	0.0	0.0	17.539627378460956	0.0
33	1.9252947144884203E-4	0.0	0.0	17.892148840683788	0.0
34	3.8505894289768406E-4	0.0	0.0	18.203661525488013	0.0
35	3.8505894289768406E-4	0.0	0.0	18.582174466356438	0.0
36	3.8505894289768406E-4	0.0	0.0	18.951638522066766	0.0
37	3.8505894289768406E-4	0.0	0.0	19.307047926361328	0.0
38	3.8505894289768406E-4	0.0	0.0	19.703658637545942	0.0
39	3.8505894289768406E-4	0.0	0.0	20.07177498695613	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGA	35	1.21021E-7	45.000004	32
ACTGCCG	35	1.21021E-7	45.000004	1
CACGGTT	35	1.21021E-7	45.000004	25
GGCAACG	35	1.21021E-7	45.000004	1
CGCGCCT	35	1.21021E-7	45.000004	17
TAGCCGT	35	1.21021E-7	45.000004	44
TACGCAG	70	0.0	45.000004	1
CGTAGTG	35	1.21021E-7	45.000004	28
CGAGGAC	35	1.21021E-7	45.000004	3
TTGCGCG	35	1.21021E-7	45.000004	14
ACGTACG	35	1.21021E-7	45.000004	1
GCCCGCC	35	1.21021E-7	45.000004	3
TCACGAG	55	1.8189894E-12	45.0	1
TCGCCTG	25	3.8878432E-5	45.0	43
CTTCGTT	20	7.029711E-4	45.0	35
CTAGATT	25	3.8878432E-5	45.0	39
CGGGTAT	40	6.8030204E-9	45.0	6
GACAGGT	25	3.8878432E-5	45.0	39
CAGCGCG	45	3.8380676E-10	45.0	1
AGCCGAG	25	3.8878432E-5	45.0	1
>>END_MODULE