FastQCFastQC Report
Sat 18 Jun 2016
SRR3548596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559713
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146962.62563135035277No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT92121.6458434947910803No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC80771.4430609973325614No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT67671.209012476036826RNA PCR Primer, Index 14 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35340.6313950185184192No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25540.45630528503000645No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT24670.44076160460807595Illumina PCR Primer Index 7 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT20560.3673311143389559No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT16610.29675923196352416No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13910.24852022375753288No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT12280.21939815584058256No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG10940.19545731473094244Illumina PCR Primer Index 7 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC9520.17008716967445817No Hit
TCGCATGGGAGTGTAGGTTTGTAGCTTTCAGTTATCGACAGTTGTATGTTT5830.10416052512627007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG302.1634987E-645.00000433
TCGCACG302.1634987E-645.0000041
TCCGCTA302.1634987E-645.00000424
CTATCCG302.1634987E-645.0000041
GCGAACG302.1634987E-645.00000410
CTATAGG600.045.0000042
CGCTATC302.1634987E-645.00000426
TATTCGA302.1634987E-645.00000419
TCTCGAG302.1634987E-645.00000414
CTCGAGT302.1634987E-645.00000415
CACGTCG302.1634987E-645.00000432
CGATATG302.1634987E-645.0000041
CCGTTCA302.1634987E-645.00000416
GCGTAAG302.1634987E-645.0000041
TGATCGA453.8380676E-1045.036
ACAACGT253.8882317E-545.018
CGGCGAA253.8882317E-545.030
GGTACGT453.8380676E-1045.09
TCGTTAC207.030181E-445.043
CAAACGA253.8882317E-545.043
Produced by FastQC (version 0.11.5)