##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559713
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526946845972848	31.0	31.0	34.0	30.0	34.0
2	31.728491208887412	31.0	31.0	34.0	30.0	34.0
3	31.85923500079505	31.0	31.0	34.0	30.0	34.0
4	35.5681983445087	37.0	35.0	37.0	33.0	37.0
5	35.30347517388376	37.0	35.0	37.0	33.0	37.0
6	33.96847134156255	37.0	35.0	37.0	31.0	37.0
7	34.678033206303944	37.0	35.0	37.0	32.0	37.0
8	35.40231332843797	37.0	35.0	37.0	32.0	37.0
9	37.126659555879534	39.0	37.0	39.0	34.0	39.0
10	36.556383360758105	38.0	35.0	39.0	32.0	39.0
11	36.56127158025631	38.0	35.0	39.0	32.0	39.0
12	36.40484855631368	38.0	35.0	39.0	32.0	39.0
13	36.449455345864756	38.0	35.0	39.0	32.0	39.0
14	37.52457777468095	39.0	36.0	41.0	32.0	41.0
15	37.58685254764495	39.0	36.0	41.0	32.0	41.0
16	37.38830257649903	39.0	36.0	41.0	32.0	41.0
17	37.35253424522925	39.0	36.0	40.0	32.0	41.0
18	37.111121235347404	39.0	36.0	40.0	32.0	41.0
19	36.986516303891456	39.0	35.0	40.0	31.0	41.0
20	37.023385199200305	39.0	35.0	40.0	31.0	41.0
21	37.05219996676868	39.0	35.0	40.0	31.0	41.0
22	36.98512094591335	39.0	35.0	40.0	31.0	41.0
23	36.83203713331654	39.0	35.0	40.0	31.0	41.0
24	36.6623716083064	39.0	35.0	40.0	31.0	41.0
25	36.75108314439722	39.0	35.0	40.0	31.0	41.0
26	36.61608538661778	39.0	35.0	40.0	31.0	41.0
27	36.53718959538192	39.0	35.0	40.0	30.0	41.0
28	36.465517148967415	38.0	35.0	40.0	30.0	41.0
29	36.33274553208519	38.0	35.0	40.0	30.0	41.0
30	36.35878387673683	38.0	35.0	40.0	30.0	41.0
31	36.25914352534245	38.0	35.0	40.0	30.0	41.0
32	36.108825415882784	38.0	35.0	40.0	30.0	41.0
33	35.97525517542026	38.0	35.0	40.0	29.0	41.0
34	35.894354785398946	38.0	35.0	40.0	29.0	41.0
35	35.75824038391104	38.0	35.0	40.0	28.0	41.0
36	35.771575789735095	38.0	35.0	40.0	29.0	41.0
37	35.72079976702346	38.0	35.0	40.0	28.0	41.0
38	35.57268099901199	38.0	35.0	40.0	27.0	41.0
39	35.528613771700854	38.0	34.0	40.0	27.0	41.0
40	35.18600604238244	38.0	34.0	40.0	26.0	41.0
41	35.147125401768406	38.0	34.0	40.0	26.0	41.0
42	35.08526334031906	38.0	34.0	40.0	26.0	41.0
43	35.09188101759295	38.0	34.0	40.0	26.0	41.0
44	34.99022177437365	38.0	34.0	40.0	25.0	41.0
45	34.779320830497056	37.0	33.0	40.0	24.0	41.0
46	34.56859318972402	37.0	33.0	40.0	24.0	41.0
47	34.45794005141921	37.0	33.0	40.0	23.0	41.0
48	34.325856287061406	37.0	33.0	40.0	23.0	41.0
49	34.17828958769941	37.0	33.0	40.0	23.0	41.0
50	34.06803308123985	37.0	33.0	40.0	23.0	41.0
51	32.67611257912537	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	2.0
12	11.0
13	13.0
14	17.0
15	43.0
16	101.0
17	206.0
18	425.0
19	847.0
20	1468.0
21	2415.0
22	3262.0
23	4124.0
24	4568.0
25	5099.0
26	5835.0
27	6776.0
28	8262.0
29	10598.0
30	13546.0
31	17199.0
32	22633.0
33	29477.0
34	40634.0
35	54876.0
36	56155.0
37	72860.0
38	100352.0
39	97887.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.78460389521058	36.774203922367356	18.394784469897964	25.0464077125241
2	21.749539496134627	37.8301022130985	17.68352709334963	22.73683119741725
3	18.81285587434989	32.83611422282491	25.072671172547363	23.27835873027784
4	23.99711995254711	33.478050357951304	17.7667840482533	24.758045641248284
5	16.58028310937927	40.76964444277692	16.359276986598488	26.290795461245313
6	17.4044554977283	40.60420251092971	22.811155002653148	19.180186988688845
7	74.96913596789784	15.572087837874054	5.240721584097565	4.218054610130549
8	76.9930303566292	9.185243151400806	3.8337505114228185	9.987975980547173
9	74.87283661447921	11.839996569670527	7.515101489513376	5.77206532633689
10	32.786088584685366	33.67833157350285	15.911904851236258	17.623674990575527
11	22.844743645403984	34.52287154309441	23.162406447590104	19.469978363911505
12	24.209192925660115	29.074186234731016	29.778833080525196	16.937787759083673
13	24.075910332616896	29.895142689199645	25.39998177637468	20.628965201808786
14	18.272578982442788	30.275873528040265	31.3121188895023	20.13942860001465
15	17.458411721721667	33.32189890175858	26.20807449532171	23.011614881198042
16	19.367783131712144	32.69479179508069	28.248763205428496	19.688661867778663
17	18.241134295612216	31.9930035571802	27.517316910630985	22.248545236576604
18	19.602010316001238	31.555993875432588	26.450877503291863	22.39111830527431
19	20.924295129825467	32.94920789761896	27.34222717714257	18.784269795413007
20	21.0920596805863	36.538011445151355	24.54311406024159	17.826814814020757
21	20.819777278712483	29.89460670021958	27.997205710783916	21.28841031028402
22	20.014721830652498	32.33174859258227	26.458917337992865	21.194612238772372
23	20.317734267383464	31.024471470199906	25.96294886843793	22.6948453939787
24	17.4836032037848	35.9975558902509	26.138395927913056	20.38044497805125
25	20.861941745144385	30.91387907731284	26.648478773943076	21.5757004035997
26	22.778817000855796	32.446450234316515	22.763273320433868	22.011459444393825
27	19.205735796738686	31.520975928734906	27.876072201288878	21.397216073237534
28	22.15242454615133	30.6701827543759	24.46289437622496	22.71449832324781
29	18.900043415107383	34.36654142390832	24.550081916982453	22.183333244001837
30	21.616256903091404	29.537459376501886	26.167696658823363	22.678587061583347
31	23.245663402493776	30.90780453553875	22.14134744056329	23.705184621404186
32	20.5875153873503	29.626790873179647	24.88025112870346	24.90544261076659
33	22.852604817111626	30.576027356877546	24.680863228118696	21.890504597892136
34	19.3872573979879	29.58998629654841	25.990462969414686	25.032293336049012
35	20.77118094451978	30.836160675203182	26.55646733236498	21.836191047912052
36	18.75032382667546	29.632686751960378	26.865018321889966	24.751971099474197
37	19.165715286227048	30.452749891462233	25.659936431706964	24.721598390603756
38	21.60285717858974	32.57383694857901	23.719477660872627	22.10382821195863
39	18.880033159851568	28.059559095464998	27.561803995976508	25.498603748706927
40	21.050252540141106	32.66709903111059	24.980302404982556	21.30234602376575
41	19.00009469138648	29.490292346256027	30.474010787671542	21.035602174685955
42	22.130448997968603	30.690193009631724	25.05980743702576	22.119550555373916
43	21.1583436511212	28.089574478348727	27.752794735873564	22.999287134656512
44	20.557678667459932	26.502511108371614	28.164434272564687	24.775375951603767
45	23.88849285258695	29.117422679123052	25.403555036241787	21.59052943204821
46	19.071738551722042	28.573393864355484	31.386085368751488	20.96878221517099
47	21.73739041258645	31.19616660681456	25.113049009045707	21.95339397155328
48	19.252009512017768	27.787812682571243	31.245477592980684	21.714700212430298
49	20.736162997822095	28.447079128053126	27.59289135682037	23.223866517304405
50	20.38812748676554	28.07331614595337	26.585232074295217	24.953324292985872
51	18.88342775672532	27.150164459285385	30.384679290993777	23.58172849299552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16827.0
1	12631.5
2	8436.0
3	5327.0
4	2218.0
5	1803.5
6	1389.0
7	1413.0
8	1437.0
9	1570.5
10	1704.0
11	1894.0
12	2084.0
13	2202.0
14	2320.0
15	2414.0
16	2508.0
17	2355.5
18	2203.0
19	2168.0
20	2133.0
21	2376.0
22	2619.0
23	3099.5
24	3580.0
25	4447.5
26	6015.5
27	6716.0
28	7737.5
29	8759.0
30	9289.0
31	9819.0
32	10796.0
33	11773.0
34	13511.0
35	15249.0
36	15843.0
37	16437.0
38	17223.5
39	18010.0
40	19852.5
41	21695.0
42	23954.5
43	26214.0
44	38327.5
45	50441.0
46	50790.0
47	51139.0
48	49534.0
49	47929.0
50	44779.5
51	41630.0
52	38628.0
53	35626.0
54	32499.0
55	29372.0
56	27498.5
57	25625.0
58	23394.5
59	21164.0
60	19275.5
61	17387.0
62	16369.5
63	15352.0
64	13203.0
65	11054.0
66	9440.0
67	7826.0
68	6461.5
69	5097.0
70	4056.0
71	3015.0
72	2780.5
73	2546.0
74	2293.0
75	1642.0
76	1244.0
77	889.5
78	535.0
79	596.5
80	658.0
81	502.0
82	346.0
83	251.0
84	156.0
85	106.0
86	56.0
87	35.0
88	14.0
89	10.5
90	7.0
91	6.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.480526586303142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5104086414773	19.21976146049209
2	8.742555325045647	4.2804471613401205
3	3.234642098930083	2.3755722570009987
4	1.6555376492338867	1.621137337467839
5	1.005950099808971	1.2313094081433908
6	0.7019522328624804	1.0310496179342885
7	0.5027619749058301	0.8615514525285112
8	0.40078261672572124	0.7849095603265728
9	0.3091689243163558	0.6811756264246763
>10	3.3736120790954254	19.159456818059393
>50	0.9941334651949731	17.352020069120993
>100	0.5582385412517094	21.309592721806624
>500	0.0014651930216580298	0.2752922473318732
>1k	0.005860772086632119	2.8668055854071612
>5k	0.002197789532487045	4.314286841573643
>10k+	7.325965108290149E-4	2.6356318350418295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14696	2.62563135035277	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	9212	1.6458434947910803	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	8077	1.4430609973325614	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	6767	1.209012476036826	RNA PCR Primer, Index 14 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3534	0.6313950185184192	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2554	0.45630528503000645	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	2467	0.44076160460807595	Illumina PCR Primer Index 7 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	2056	0.3673311143389559	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1661	0.29675923196352416	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1391	0.24852022375753288	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT	1228	0.21939815584058256	No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1094	0.19545731473094244	Illumina PCR Primer Index 7 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC	952	0.17008716967445817	No Hit
TCGCATGGGAGTGTAGGTTTGTAGCTTTCAGTTATCGACAGTTGTATGTTT	583	0.10416052512627007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5481380636147454	0.0
2	0.0	0.0	0.0	0.667842269162946	0.0
3	0.0	0.0	0.0	1.040354610309212	0.0
4	0.0	0.0	0.0	2.985101292984083	0.0
5	0.0	0.0	0.0	3.1753773809077153	0.0
6	0.0	0.0	0.0	4.144445456868073	0.0
7	0.0	0.0	0.0	4.943247700160618	0.0
8	0.0	0.0	0.0	5.296821764011199	0.0
9	0.0	0.0	0.0	7.66499974093866	0.0
10	0.0	0.0	0.0	8.509718373523574	0.0
11	0.0	0.0	0.0	10.926135358657026	0.0
12	0.0	0.0	0.0	11.343670774128883	0.0
13	0.0	0.0	0.0	11.587903086045884	0.0
14	0.0	0.0	0.0	12.01151304329183	0.0
15	0.0	0.0	0.0	12.234484458999523	0.0
16	0.0	0.0	0.0	12.587522533870038	0.0
17	0.0	0.0	0.0	12.947171139494705	0.0
18	0.0	0.0	0.0	13.70488089431548	0.0
19	0.0	0.0	0.0	13.949649195212546	0.0
20	0.0	0.0	0.0	14.183519053514926	0.0
21	0.0	0.0	0.0	14.459017389269142	0.0
22	0.0	0.0	0.0	14.745414167618048	0.0
23	0.0	0.0	0.0	15.052535853196192	0.0
24	0.0	0.0	0.0	15.251387764800889	0.0
25	0.0	0.0	0.0	15.44613042755841	0.0
26	0.0	0.0	0.0	15.62765202880762	0.0
27	0.0	0.0	0.0	15.812389563937232	0.0
28	0.0	0.0	0.0	16.00087902192731	0.0
29	0.0	0.0	0.0	16.20562681231274	0.0
30	0.0	0.0	0.0	16.468082749552003	0.0
31	1.7866299335552328E-4	0.0	0.0	16.67300920293079	0.0
32	1.7866299335552328E-4	0.0	0.0	16.873826407462396	0.0
33	1.7866299335552328E-4	0.0	0.0	17.089472640442512	0.0
34	1.7866299335552328E-4	0.0	0.0	17.284929955173453	0.0
35	1.7866299335552328E-4	0.0	0.0	17.50593607795424	0.0
36	1.7866299335552328E-4	0.0	0.0	17.70067874071176	0.0
37	1.7866299335552328E-4	0.0	0.0	17.902210597216786	0.0
38	1.7866299335552328E-4	0.0	0.0	18.108387691549062	0.0
39	1.7866299335552328E-4	0.0	0.0	18.33886295297769	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	30	2.1634987E-6	45.000004	33
TCGCACG	30	2.1634987E-6	45.000004	1
TCCGCTA	30	2.1634987E-6	45.000004	24
CTATCCG	30	2.1634987E-6	45.000004	1
GCGAACG	30	2.1634987E-6	45.000004	10
CTATAGG	60	0.0	45.000004	2
CGCTATC	30	2.1634987E-6	45.000004	26
TATTCGA	30	2.1634987E-6	45.000004	19
TCTCGAG	30	2.1634987E-6	45.000004	14
CTCGAGT	30	2.1634987E-6	45.000004	15
CACGTCG	30	2.1634987E-6	45.000004	32
CGATATG	30	2.1634987E-6	45.000004	1
CCGTTCA	30	2.1634987E-6	45.000004	16
GCGTAAG	30	2.1634987E-6	45.000004	1
TGATCGA	45	3.8380676E-10	45.0	36
ACAACGT	25	3.8882317E-5	45.0	18
CGGCGAA	25	3.8882317E-5	45.0	30
GGTACGT	45	3.8380676E-10	45.0	9
TCGTTAC	20	7.030181E-4	45.0	43
CAAACGA	25	3.8882317E-5	45.0	43
>>END_MODULE