FastQCFastQC Report
Sat 18 Jun 2016
SRR3548593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797883
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198492.48770809755315No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45590.5713870329359066No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36010.4513193037074358No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21160.26520179023741575No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT18930.23725283030218716Illumina PCR Primer Index 11 (95% over 23bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10220.12808895539822254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT551.8189894E-1245.00000423
ATTCGCG551.8189894E-1245.0000041
CATTCTA1250.045.00000445
TGGGCGA700.045.0000046
TGCGTGA351.2113014E-745.00000431
CGGACAA253.889729E-545.019
ACAACGT253.889729E-545.012
TCGCATA253.889729E-545.028
CTCGTAA253.889729E-545.029
GCGAAAT207.0319884E-445.023
GTTTACG207.0319884E-445.01
TAGGTCC207.0319884E-445.044
CGTTGCA406.8102963E-945.039
CGCATAT253.889729E-545.029
CGTTGAT1050.045.025
TTCACGG453.8380676E-1045.02
ACCGAAA207.0319884E-445.010
TCGATCC207.0319884E-445.030
ATACTCG253.889729E-545.044
CGTTAGA207.0319884E-445.09