##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797883
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60165337524424	31.0	31.0	34.0	30.0	34.0
2	31.806778688103392	33.0	31.0	34.0	30.0	34.0
3	31.98930670286245	33.0	31.0	34.0	30.0	34.0
4	35.72324639076155	37.0	35.0	37.0	33.0	37.0
5	35.484163718239394	37.0	35.0	37.0	33.0	37.0
6	32.6834485757937	37.0	35.0	37.0	26.0	37.0
7	34.17630780452773	37.0	35.0	37.0	27.0	37.0
8	35.26492104732147	37.0	35.0	37.0	32.0	37.0
9	37.1192881663101	39.0	37.0	39.0	34.0	39.0
10	36.659071568137186	38.0	37.0	39.0	32.0	39.0
11	36.67034765748863	39.0	37.0	39.0	32.0	39.0
12	36.57515826255228	39.0	35.0	39.0	32.0	39.0
13	36.578824213575174	39.0	35.0	39.0	32.0	39.0
14	37.720520427180425	39.0	37.0	41.0	33.0	41.0
15	37.72938513541459	40.0	37.0	41.0	33.0	41.0
16	37.674128913637716	39.0	36.0	41.0	33.0	41.0
17	37.65481279836768	39.0	36.0	41.0	33.0	41.0
18	37.50263885807819	39.0	36.0	41.0	32.0	41.0
19	37.4405896603888	39.0	35.0	41.0	32.0	41.0
20	37.29449555887267	39.0	35.0	41.0	32.0	41.0
21	37.24573151702693	39.0	35.0	40.0	32.0	41.0
22	37.15209623466097	39.0	35.0	40.0	32.0	41.0
23	37.00776680290218	39.0	35.0	40.0	31.0	41.0
24	36.842440558327475	39.0	35.0	40.0	31.0	41.0
25	36.84888761886141	39.0	35.0	40.0	31.0	41.0
26	36.77922828284348	39.0	35.0	40.0	31.0	41.0
27	36.68171899890084	39.0	35.0	40.0	31.0	41.0
28	36.602946797964115	39.0	35.0	40.0	31.0	41.0
29	36.42320365266587	38.0	35.0	40.0	30.0	41.0
30	36.432117240247	38.0	35.0	40.0	30.0	41.0
31	36.05579138796039	38.0	35.0	40.0	30.0	41.0
32	36.128847211934584	38.0	35.0	40.0	30.0	41.0
33	36.10047463099227	38.0	35.0	40.0	30.0	41.0
34	36.060116583509114	38.0	35.0	40.0	30.0	41.0
35	36.00589434791818	38.0	35.0	40.0	30.0	41.0
36	35.903003322542276	38.0	35.0	40.0	29.0	41.0
37	35.8144051195476	38.0	35.0	40.0	29.0	41.0
38	35.72133633627988	38.0	35.0	40.0	29.0	41.0
39	35.600494057399395	38.0	34.0	40.0	28.0	41.0
40	35.4320608410005	38.0	34.0	40.0	27.0	41.0
41	35.377201920582344	38.0	34.0	40.0	27.0	41.0
42	35.24738464160785	38.0	34.0	40.0	27.0	41.0
43	35.165906530155425	38.0	34.0	40.0	26.0	41.0
44	35.00788085471178	38.0	34.0	40.0	26.0	41.0
45	34.84334294627157	37.0	34.0	40.0	25.0	41.0
46	34.58059890986523	37.0	33.0	40.0	24.0	41.0
47	34.50311887833178	37.0	33.0	40.0	24.0	41.0
48	34.486813229508584	37.0	33.0	40.0	24.0	41.0
49	34.35681547294528	37.0	33.0	40.0	24.0	41.0
50	34.23704227311523	36.0	33.0	40.0	24.0	41.0
51	33.13163834797834	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	10.0
11	20.0
12	28.0
13	34.0
14	70.0
15	109.0
16	179.0
17	380.0
18	717.0
19	1204.0
20	1802.0
21	2692.0
22	3687.0
23	4613.0
24	5765.0
25	6724.0
26	8068.0
27	9498.0
28	11757.0
29	14680.0
30	18303.0
31	23514.0
32	30598.0
33	40584.0
34	59522.0
35	79421.0
36	82623.0
37	108844.0
38	143058.0
39	139344.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.027247102645376	33.240963900722285	19.42314850673595	27.30864048989639
2	23.301286028151996	32.10119779466413	19.576178462255744	25.021337714928126
3	20.898678126993556	33.83077468751684	19.048908173253473	26.221639012236132
4	19.748133498269794	34.45505168050955	18.514493979693764	27.282320841526893
5	18.305064777667905	37.256840915272036	15.658436136626547	28.779658170433507
6	16.637527055971866	44.88615498763603	17.731797769848463	20.744520186543642
7	81.66823957898589	10.50705429242132	4.2016185330430655	3.6230875955497233
8	82.93659596707788	9.554659016422207	3.8095811039964502	3.699163912503462
9	76.77804891193321	12.65423627273673	6.383392051215529	4.184322764114539
10	36.49394710753331	33.75118908411384	13.356218894248906	16.398644914103947
11	26.233545519831853	32.62270282735689	22.756970633539	18.38678101927225
12	25.814937779097942	28.660843757794062	26.324034977559368	19.20018348554863
13	24.28213159072195	28.82552955759178	25.34657336977978	21.545765481906496
14	21.853454704511815	30.506728430108176	26.237932127893437	21.401884737486572
15	20.470043853547452	33.364540916400024	25.708656532348726	20.456758697703798
16	22.282715636252433	31.78561267754796	25.475915641767028	20.45575604443258
17	21.102091409392106	31.5761834755221	26.282926193439387	21.038798921646407
18	21.895440810244107	31.875099482004256	24.804263281709225	21.425196426042415
19	20.718701864809752	33.951469075039824	24.24139880157868	21.088430258571744
20	22.79431946789191	31.91746158271325	25.571543697509536	19.716675251885302
21	23.561725215351125	32.191687252391645	25.1204750571199	19.126112475137333
22	22.23195631439697	31.06959291023872	24.585058210288977	22.11339256507533
23	21.944570820533837	31.358482384008685	24.462859842859167	22.234086952598314
24	20.320272521159115	32.482582032704045	25.58595683828331	21.61118860785353
25	21.176037088144504	33.11851486997467	24.155295951912752	21.550152089968076
26	23.004625991530087	32.56229296776595	21.824879086282074	22.60820195442189
27	20.74915745792303	31.54109061102944	24.747864035203158	22.96188789584438
28	20.054820067603895	32.33882661994302	26.072745001460113	21.533608310992967
29	21.15297606290647	31.84652386377451	23.947872056429325	23.052628016889695
30	21.308512651604307	32.384447343783485	23.764887834431867	22.54215217018034
31	21.507288662623466	32.05482508087025	22.36505853615129	24.07282772035499
32	21.84430549341194	30.786468692778264	24.448446702085395	22.9207791117244
33	20.882761006312954	31.73046674763092	24.464238491107093	22.922533754949033
34	19.626436457475595	30.63607070209542	26.441846736927594	23.29564610350139
35	21.60354337666049	30.701869823019162	24.647473376422358	23.04711342389799
36	21.091062223408695	32.7075523604338	25.039636137127875	21.16174927902963
37	19.98663964516101	32.552642430030474	24.638574828640287	22.822143096168233
38	21.25111075182702	30.63118276739823	25.901541955399477	22.216164525375273
39	20.76657855851046	29.758623758119924	25.90066463378716	23.574133049582457
40	21.275550425312982	31.044401246799342	26.00656988555966	21.67347844232801
41	19.917707232764702	29.671894250159486	28.447403942683323	21.962994574392486
42	21.417425863190466	30.399695193405552	25.02935894109788	23.153520002306102
43	20.477313089763786	29.3291121630615	26.902941910029416	23.290632837145296
44	22.09283817301534	28.63214280790542	24.78822082936972	24.486798189709518
45	21.254369374958486	29.546186596280403	25.557255888394664	23.642188140366443
46	20.118237887008497	30.891371291279548	26.073246328095724	22.91714449361623
47	20.260739983180493	30.051523844974763	27.31941901256199	22.368317159282753
48	20.485710310910246	28.652321204988702	28.179820850926763	22.68214763317429
49	19.76104265913674	30.53029078198182	26.625833612196274	23.082832946685166
50	20.726723090979505	28.379348851899337	26.955330543450607	23.93859751367055
51	21.068126529829563	27.901083241527893	27.143077368486356	23.88771286015619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23024.0
1	17294.0
2	11564.0
3	7316.5
4	3069.0
5	2717.0
6	2365.0
7	2228.5
8	2092.0
9	2342.5
10	2593.0
11	2719.5
12	2846.0
13	2970.5
14	3095.0
15	3513.5
16	3932.0
17	3853.5
18	3775.0
19	3681.5
20	3588.0
21	3587.5
22	3587.0
23	4449.0
24	5311.0
25	5390.0
26	6807.0
27	8145.0
28	9781.5
29	11418.0
30	13492.5
31	15567.0
32	18038.5
33	20510.0
34	21781.0
35	23052.0
36	26571.5
37	30091.0
38	30410.5
39	30730.0
40	32030.0
41	33330.0
42	33794.5
43	34259.0
44	38530.5
45	42802.0
46	45625.0
47	48448.0
48	53582.0
49	58716.0
50	56661.5
51	54607.0
52	52304.5
53	50002.0
54	47343.0
55	44684.0
56	41390.5
57	38097.0
58	36259.5
59	34422.0
60	32278.5
61	30135.0
62	29276.5
63	28418.0
64	24297.0
65	20176.0
66	18970.0
67	17764.0
68	16254.0
69	14744.0
70	12374.5
71	10005.0
72	8560.0
73	7115.0
74	6313.5
75	4584.5
76	3657.0
77	2907.0
78	2157.0
79	1649.5
80	1142.0
81	965.0
82	788.0
83	692.0
84	596.0
85	380.5
86	165.0
87	174.5
88	184.0
89	116.0
90	48.0
91	38.0
92	28.0
93	25.5
94	23.0
95	21.0
96	19.0
97	18.0
98	17.0
99	8.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	797883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.195243342569892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51211398838038	17.979123459574982
2	8.949761749639148	4.151838032818081
3	3.3839336618194027	2.35473494223044
4	1.7000831800938487	1.577353722595476
5	1.107221599944818	1.284113722243481
6	0.8056376099434653	1.121217624513905
7	0.5724185957141912	0.9294172034981958
8	0.443517029299461	0.8229988336939758
9	0.38094428220761883	0.7952485793189703
>10	3.25028587713165	16.88931777724011
>50	1.0726187680705626	17.96461063068767
>100	0.8154315481190819	29.568119709772024
>500	0.002741868016540289	0.35933037159126713
>1k	0.002741868016540289	1.6778502412957186
>5k	0.0	0.0
>10k+	5.483736033080578E-4	2.5247251489256857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19849	2.48770809755315	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4559	0.5713870329359066	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3601	0.4513193037074358	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2116	0.26520179023741575	No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	1893	0.23725283030218716	Illumina PCR Primer Index 11 (95% over 23bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1022	0.12808895539822254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29327608183154674	0.0
2	0.0	0.0	0.0	0.4009359768286829	0.0
3	0.0	0.0	0.0	0.5097238567559405	0.0
4	0.0	0.0	0.0	1.0947720405122054	0.0
5	0.0	0.0	0.0	1.1850108349219122	0.0
6	0.0	0.0	0.0	1.7861014710176806	0.0
7	0.0	0.0	0.0	2.2483246290496224	0.0
8	0.0	0.0	0.0	2.5795762035285876	0.0
9	0.0	0.0	0.0	3.0837854672928238	0.0
10	0.0	0.0	0.0	3.8637243806422745	0.0
11	0.0	0.0	0.0	4.561821720728478	0.0
12	0.0	0.0	0.0	4.920771591824867	0.0
13	0.0	0.0	0.0	5.0681616226940545	0.0
14	0.0	0.0	0.0	5.210287723889342	0.0
15	0.0	0.0	0.0	5.359933724618773	0.0
16	0.0	0.0	0.0	5.645063248621665	0.0
17	0.0	0.0	0.0	5.996743883501717	0.0
18	0.0	0.0	0.0	6.48440936829084	0.0
19	1.253316589023704E-4	0.0	0.0	6.676292138070369	0.0
20	1.253316589023704E-4	0.0	0.0	6.893993229583786	0.0
21	1.253316589023704E-4	0.0	0.0	7.136635321218775	0.0
22	1.253316589023704E-4	0.0	0.0	7.422892830151789	0.0
23	1.253316589023704E-4	0.0	0.0	7.685086660575548	0.0
24	1.253316589023704E-4	0.0	0.0	7.9084276767395725	0.0
25	1.253316589023704E-4	0.0	0.0	8.099934451542394	0.0
26	1.253316589023704E-4	0.0	0.0	8.257601678441576	0.0
27	1.253316589023704E-4	0.0	0.0	8.438831257214403	0.0
28	1.253316589023704E-4	0.0	0.0	8.632468670218566	0.0
29	1.253316589023704E-4	0.0	0.0	8.837887259159551	0.0
30	1.253316589023704E-4	0.0	0.0	9.083913305584904	0.0
31	1.253316589023704E-4	0.0	0.0	9.262134924544075	0.0
32	2.506633178047408E-4	0.0	0.0	9.454268357641409	0.0
33	2.506633178047408E-4	0.0	0.0	9.659812278241295	0.0
34	2.506633178047408E-4	0.0	0.0	9.857460304330335	0.0
35	2.506633178047408E-4	0.0	0.0	10.065886853084976	0.0
36	2.506633178047408E-4	0.0	0.0	10.26178023594938	0.0
37	2.506633178047408E-4	0.0	0.0	10.450404382597448	0.0
38	2.506633178047408E-4	0.0	0.0	10.652313685089167	0.0
39	2.506633178047408E-4	0.0	0.0	10.8708920982149	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	55	1.8189894E-12	45.000004	23
ATTCGCG	55	1.8189894E-12	45.000004	1
CATTCTA	125	0.0	45.000004	45
TGGGCGA	70	0.0	45.000004	6
TGCGTGA	35	1.2113014E-7	45.000004	31
CGGACAA	25	3.889729E-5	45.0	19
ACAACGT	25	3.889729E-5	45.0	12
TCGCATA	25	3.889729E-5	45.0	28
CTCGTAA	25	3.889729E-5	45.0	29
GCGAAAT	20	7.0319884E-4	45.0	23
GTTTACG	20	7.0319884E-4	45.0	1
TAGGTCC	20	7.0319884E-4	45.0	44
CGTTGCA	40	6.8102963E-9	45.0	39
CGCATAT	25	3.889729E-5	45.0	29
CGTTGAT	105	0.0	45.0	25
TTCACGG	45	3.8380676E-10	45.0	2
ACCGAAA	20	7.0319884E-4	45.0	10
TCGATCC	20	7.0319884E-4	45.0	30
ATACTCG	25	3.889729E-5	45.0	44
CGTTAGA	20	7.0319884E-4	45.0	9
>>END_MODULE