FastQCFastQC Report
Sat 18 Jun 2016
SRR3548591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957553
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196642.053567792069995No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54830.5726053805898994No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34430.3595623427632726No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT24720.25815803407226545No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23000.24019558186335377No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG12330.12876571845109355No Hit
TAGTATGGGGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAAC11690.12208201530359156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA253.8903156E-545.00000419
GTCGCTA253.8903156E-545.00000421
GGTACCG253.8903156E-545.00000420
ACACGAC600.045.00000426
GATCGGT253.8903156E-545.00000424
GACCGTA302.1651213E-645.00000416
ATTACCG302.1651213E-645.00000415
GCGCCTA253.8903156E-545.0000049
TTCGTAC502.1827873E-1145.00000433
CGACACC302.1651213E-645.00000433
TCGGCCC302.1651213E-645.00000435
TAACACG302.1651213E-645.00000434
GTCCGAT253.8903156E-545.00000412
TCGTATA253.8903156E-545.00000430
TCGTACA502.1827873E-1145.00000434
CGTCGTT302.1651213E-645.00000430
TTACGGC302.1651213E-645.00000422
GCGGATA302.1651213E-645.00000413
TAGGCCG302.1651213E-645.00000423
CACGTTA302.1651213E-645.0000045