##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957553
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654524605948705	31.0	31.0	34.0	30.0	34.0
2	31.853805481263176	33.0	31.0	34.0	30.0	34.0
3	32.028330546716475	33.0	31.0	34.0	30.0	34.0
4	35.755068387859474	37.0	35.0	37.0	35.0	37.0
5	35.51539183731867	37.0	35.0	37.0	33.0	37.0
6	32.61337074814658	37.0	35.0	37.0	26.0	37.0
7	34.18209435926784	37.0	35.0	37.0	26.0	37.0
8	35.32570938632117	37.0	35.0	37.0	32.0	37.0
9	37.19652593642336	39.0	37.0	39.0	34.0	39.0
10	36.715253359344075	39.0	37.0	39.0	32.0	39.0
11	36.761882632084074	39.0	37.0	39.0	32.0	39.0
12	36.71407535666433	39.0	35.0	39.0	32.0	39.0
13	36.68038635981507	39.0	35.0	39.0	32.0	39.0
14	37.8418259876999	40.0	37.0	41.0	33.0	41.0
15	37.89192765309074	40.0	37.0	41.0	33.0	41.0
16	37.86884381334506	40.0	37.0	41.0	33.0	41.0
17	37.807631535800105	39.0	37.0	41.0	33.0	41.0
18	37.677511323133025	39.0	36.0	41.0	33.0	41.0
19	37.642535713427876	39.0	36.0	41.0	33.0	41.0
20	37.504501578502705	39.0	36.0	41.0	32.0	41.0
21	37.44011349763407	39.0	36.0	41.0	32.0	41.0
22	37.40740094804152	39.0	36.0	41.0	32.0	41.0
23	37.23488203786109	39.0	35.0	41.0	32.0	41.0
24	37.095934115396226	39.0	35.0	41.0	31.0	41.0
25	37.1167110332274	39.0	35.0	41.0	31.0	41.0
26	37.02557665215398	39.0	35.0	41.0	31.0	41.0
27	36.948017498770305	39.0	35.0	40.0	31.0	41.0
28	36.91170514843565	39.0	35.0	40.0	31.0	41.0
29	36.717924751945844	39.0	35.0	40.0	31.0	41.0
30	36.77069363262399	39.0	35.0	40.0	31.0	41.0
31	36.3558957049897	39.0	35.0	40.0	30.0	41.0
32	36.466003448373094	39.0	35.0	40.0	30.0	41.0
33	36.44838144729326	39.0	35.0	40.0	30.0	41.0
34	36.392777214420505	39.0	35.0	40.0	30.0	41.0
35	36.28466518302381	39.0	35.0	40.0	30.0	41.0
36	36.203193974641614	38.0	35.0	40.0	30.0	41.0
37	36.12179273627674	38.0	35.0	40.0	30.0	41.0
38	36.04285298046165	38.0	35.0	40.0	30.0	41.0
39	35.972853721934975	38.0	35.0	40.0	29.0	41.0
40	35.86160870468789	38.0	35.0	40.0	29.0	41.0
41	35.81298058697534	38.0	35.0	40.0	29.0	41.0
42	35.70030483952324	38.0	35.0	40.0	28.0	41.0
43	35.61107322518962	38.0	35.0	40.0	28.0	41.0
44	35.45789214800643	38.0	34.0	40.0	27.0	41.0
45	35.25010834909399	38.0	34.0	40.0	27.0	41.0
46	35.0020949232053	38.0	34.0	40.0	26.0	41.0
47	34.96440197043923	37.0	34.0	40.0	26.0	41.0
48	34.96343178915423	37.0	34.0	40.0	26.0	41.0
49	34.80930246158698	37.0	34.0	40.0	26.0	41.0
50	34.704103062702536	37.0	33.0	40.0	26.0	41.0
51	33.59191292805725	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	20.0
11	19.0
12	27.0
13	31.0
14	67.0
15	93.0
16	162.0
17	347.0
18	687.0
19	1193.0
20	1860.0
21	2886.0
22	3838.0
23	4979.0
24	6076.0
25	7180.0
26	8398.0
27	10199.0
28	12496.0
29	15931.0
30	20193.0
31	25717.0
32	34060.0
33	46191.0
34	67068.0
35	89387.0
36	98316.0
37	133272.0
38	180189.0
39	186621.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.689403093092498	31.854842499579654	20.30874531226992	27.14700909505792
2	23.89215009508612	31.73192502138263	19.813837980769733	24.562086902761518
3	21.139926458378806	32.83640696650734	20.158466424312806	25.865200150801055
4	20.34258155945415	34.94845716111797	19.11288461317546	25.596076666252415
5	18.321492387366547	37.171206189109114	16.33121090947446	28.17609051404987
6	17.08291864784508	45.3292924778054	16.29904558807711	21.288743286272403
7	82.72053870647369	9.192911515080628	4.4049781056505495	3.681571672795135
8	84.17915248555433	8.898933009452218	3.9015072794926233	3.020407225500834
9	77.36229743941067	12.699662577423911	6.227853706270045	3.7101862768953784
10	34.80402651341492	37.121705012672926	12.538835970437145	15.535432503475002
11	23.916691817580855	31.726181213990245	26.023624801969188	18.333502166459713
12	25.088010794180583	27.624789437242637	28.17045113951917	19.116748629057607
13	23.479013694281154	28.908060441563027	26.61701232203335	20.995913542122473
14	20.468840889224932	30.541077099648795	27.651210951247606	21.33887105987867
15	19.189016169339972	34.16479296707337	26.141425069943907	20.504765793642754
16	22.376724839251718	30.45993276612365	26.499629785505345	20.663712609119287
17	19.93299587594629	31.46802317991798	27.78300522268741	20.815975721448314
18	21.203212772556714	31.892647195507717	26.310710738726733	20.593429293208835
19	21.846414767642106	32.33053418453078	25.08550440550027	20.737546642326848
20	23.38251773008909	31.800224112921168	25.538116428020174	19.27914172896957
21	22.294953908556497	31.405676761495187	26.83245731567861	19.466912014269706
22	22.515933843870783	27.93620823077156	26.602496154259867	22.94536177109779
23	20.55384923863222	32.18840105978468	25.40861967953732	21.849130022045777
24	19.147765188976486	31.678873127649332	27.953648518672075	21.21971316470211
25	20.980039747147156	32.547336805377874	24.21286341330454	22.25976003417043
26	22.854191882851392	31.48880531939224	23.949797034733326	21.707205763023037
27	20.039621827721284	31.65631562952651	26.513728221832107	21.790334320920095
28	19.852478139591227	31.24808757322049	26.92905771273235	21.97037657445593
29	20.23908859352955	32.27100745337334	25.79449910344388	21.69540484965323
30	22.08661034950546	30.027580718769613	25.971826102576046	21.913982829148882
31	22.315527182307402	31.82664562692613	23.505226342562764	22.352600848203704
32	20.876233482637517	30.231538097630107	26.00305152821828	22.889176891514097
33	21.044370389941864	30.01452661105965	25.939243049731974	23.001859949266514
34	19.80684097903719	30.06204356312392	27.7789323410819	22.352183116756983
35	21.05168069025944	29.450484725127485	27.72159870001974	21.776235884593333
36	21.570816445669326	32.23842440052926	25.109315097963247	21.081444055838165
37	21.5297743310292	31.432933738393594	24.996423174487468	22.04086875608974
38	19.598392987124473	30.614075669962915	27.023987183999214	22.763544158913398
39	20.746423435569625	29.372577810314414	27.197763465834267	22.683235288281693
40	21.182848364529168	29.863098961624058	27.41279072803281	21.541261945813968
41	20.785690191561198	28.001061037874663	28.13776365381342	23.07548511675072
42	21.690600938015965	29.047373879043768	26.22601568790448	23.036009495035785
43	20.12650996863881	30.24051932373456	27.51597039537237	22.117000312254255
44	22.156162635384153	27.71752581841423	26.672988335893677	23.453323210307943
45	22.09371178409968	29.805347589115172	27.09259957412279	21.00834105266236
46	20.75958197614127	29.18198783774893	28.230186736399972	21.828243449709834
47	20.823181588904216	31.186054453382738	26.11865870609773	21.872105251615316
48	20.076904359340944	29.71710182099581	27.865402750552708	22.340591069110534
49	19.6847589637336	29.301772330095567	27.578107948071807	23.43536075809903
50	20.13037398452096	28.646978287363726	28.300156753725382	22.922490974389927
51	20.26383918174764	28.16230537630815	27.199747690206184	24.374107751738023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23060.0
1	18000.5
2	12941.0
3	8012.0
4	3083.0
5	2577.5
6	2072.0
7	2186.5
8	2301.0
9	2378.0
10	2455.0
11	3086.0
12	3717.0
13	3552.5
14	3388.0
15	3183.0
16	2978.0
17	3283.0
18	3588.0
19	3697.5
20	3807.0
21	4543.0
22	5279.0
23	5090.5
24	4902.0
25	7353.5
26	11199.5
27	12594.0
28	14337.0
29	16080.0
30	18458.5
31	20837.0
32	23269.5
33	25702.0
34	28841.5
35	31981.0
36	33305.5
37	34630.0
38	38140.0
39	41650.0
40	42330.0
41	43010.0
42	47347.5
43	51685.0
44	53273.0
45	54861.0
46	60397.0
47	65933.0
48	68748.0
49	71563.0
50	69878.0
51	68193.0
52	62812.0
53	57431.0
54	54103.0
55	50775.0
56	47437.0
57	44099.0
58	41549.0
59	38999.0
60	35238.5
61	31478.0
62	30018.5
63	28559.0
64	25245.5
65	21932.0
66	19552.0
67	17172.0
68	15714.0
69	14256.0
70	11975.0
71	9694.0
72	8846.0
73	7998.0
74	6598.5
75	4562.0
76	3925.0
77	3018.5
78	2112.0
79	1456.0
80	800.0
81	646.0
82	492.0
83	382.5
84	273.0
85	215.0
86	157.0
87	96.5
88	36.0
89	32.5
90	29.0
91	24.0
92	19.0
93	15.5
94	12.0
95	9.5
96	7.0
97	3.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	957553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.94121046934962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33447994274096	15.985364105492852
2	9.541519770282516	3.996219474138931
3	3.638955319431663	2.2861238769833347
4	1.9176503908759122	1.6063168176785219
5	1.1805069435799738	1.2360622183018433
6	0.8013947152768195	1.0069305240981832
7	0.6275288013838318	0.919884889375026
8	0.46657632026462736	0.7816538338140987
9	0.36325768249743456	0.6846350025408262
>10	3.120523502524741	13.991712018423302
>50	0.9413343167887966	14.397671200547357
>100	1.0551442153794592	38.40297005227632
>500	0.007587326572719026	0.9161470701317839
>1k	0.0025291088575730087	1.1246078672203894
>5k	5.058217715146017E-4	0.5807878813195249
>10k+	5.058217715146017E-4	2.082913167657694
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19664	2.053567792069995	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5483	0.5726053805898994	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3443	0.3595623427632726	No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	2472	0.25815803407226545	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2300	0.24019558186335377	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1233	0.12876571845109355	No Hit
TAGTATGGGGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAAC	1169	0.12208201530359156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0886572335943808E-4	0.0	0.0	0.30807694195517116	0.0
2	2.0886572335943808E-4	0.0	0.0	0.39966456164828473	0.0
3	2.0886572335943808E-4	0.0	0.0	0.5153761723894135	0.0
4	2.0886572335943808E-4	0.0	0.0	1.0229198801528478	0.0
5	2.0886572335943808E-4	0.0	0.0	1.102393287891114	0.0
6	2.0886572335943808E-4	0.0	0.0	1.6424156156369412	0.0
7	2.0886572335943808E-4	0.0	0.0	2.090432592242936	0.0
8	2.0886572335943808E-4	0.0	0.0	2.447801844910934	0.0
9	2.0886572335943808E-4	0.0	0.0	2.9604627628966753	0.0
10	2.0886572335943808E-4	0.0	0.0	3.7820360857310247	0.0
11	2.0886572335943808E-4	0.0	0.0	4.490404186504559	0.0
12	2.0886572335943808E-4	0.0	0.0	4.848191170619277	0.0
13	2.0886572335943808E-4	0.0	0.0	4.9812386363992385	0.0
14	2.0886572335943808E-4	0.0	0.0	5.132352987249792	0.0
15	2.0886572335943808E-4	0.0	0.0	5.260700974254167	0.0
16	2.0886572335943808E-4	0.0	0.0	5.543818462267885	0.0
17	2.0886572335943808E-4	0.0	0.0	5.937008186492027	0.0
18	2.0886572335943808E-4	0.0	0.0	6.417608215942094	0.0
19	2.0886572335943808E-4	0.0	0.0	6.625220744961376	0.0
20	2.0886572335943808E-4	0.0	0.0	6.849124800402693	0.0
21	2.0886572335943808E-4	0.0	0.0	7.087753889340851	0.0
22	2.0886572335943808E-4	0.0	0.0	7.372020138833046	0.0
23	2.0886572335943808E-4	0.0	0.0	7.674144407672474	0.0
24	2.0886572335943808E-4	0.0	0.0	7.893140118614844	0.0
25	2.0886572335943808E-4	0.0	0.0	8.082059165393456	0.0
26	2.0886572335943808E-4	0.0	0.0	8.253329058548195	0.0
27	2.0886572335943808E-4	0.0	0.0	8.442352538188487	0.0
28	2.0886572335943808E-4	0.0	0.0	8.63419570509413	0.0
29	2.0886572335943808E-4	0.0	0.0	8.85037172877115	0.0
30	2.0886572335943808E-4	0.0	0.0	9.109991822906931	0.0
31	2.0886572335943808E-4	0.0	0.0	9.305907871418084	0.0
32	2.0886572335943808E-4	0.0	0.0	9.516862252011116	0.0
33	3.132985850391571E-4	0.0	0.0	9.729696424114383	0.0
34	3.132985850391571E-4	0.0	0.0	9.956629032544413	0.0
35	3.132985850391571E-4	0.0	0.0	10.18753008971827	0.0
36	3.132985850391571E-4	0.0	0.0	10.398693336034663	0.0
37	3.132985850391571E-4	0.0	0.0	10.60473937212875	0.0
38	3.132985850391571E-4	0.0	0.0	10.828121263261668	0.0
39	3.132985850391571E-4	0.0	0.0	11.074373951102446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.8903156E-5	45.000004	19
GTCGCTA	25	3.8903156E-5	45.000004	21
GGTACCG	25	3.8903156E-5	45.000004	20
ACACGAC	60	0.0	45.000004	26
GATCGGT	25	3.8903156E-5	45.000004	24
GACCGTA	30	2.1651213E-6	45.000004	16
ATTACCG	30	2.1651213E-6	45.000004	15
GCGCCTA	25	3.8903156E-5	45.000004	9
TTCGTAC	50	2.1827873E-11	45.000004	33
CGACACC	30	2.1651213E-6	45.000004	33
TCGGCCC	30	2.1651213E-6	45.000004	35
TAACACG	30	2.1651213E-6	45.000004	34
GTCCGAT	25	3.8903156E-5	45.000004	12
TCGTATA	25	3.8903156E-5	45.000004	30
TCGTACA	50	2.1827873E-11	45.000004	34
CGTCGTT	30	2.1651213E-6	45.000004	30
TTACGGC	30	2.1651213E-6	45.000004	22
GCGGATA	30	2.1651213E-6	45.000004	13
TAGGCCG	30	2.1651213E-6	45.000004	23
CACGTTA	30	2.1651213E-6	45.000004	5
>>END_MODULE