SRR3548590_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269337
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13694	5.08433672313867	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2982	1.1071631450561936	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2462	0.9140964665085006	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1481	0.549868751786795	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1297	0.48155285014684207	TruSeq Adapter, Index 20 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	1181	0.4384841295477413	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	1062	0.39430156272625	No Hit
AATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	817	0.3033374545643562	No Hit
GCATACGGGGGAGACAAAGAGAATATGATATAGGAGACACGGATAACTTTT	508	0.1886112936581309	No Hit
AAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	475	0.176358985211835	No Hit
TAATTTGGGATTATTTCAGAATCCTCTATTTCTTCCATGTTTGCTTTGCTT	400	0.14851282965207155	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	399	0.1481415475779414	No Hit
ATGATAGGGTAAAGTTGATAAAATAATCAAATCCTGAAAGGTAATGAATTA	363	0.13477539290925494	No Hit
ACGTCTGGGACTTTTCTATTGTTTGATCAAATATCTGGCAGAAGCAATTTA	348	0.12920616179730227	No Hit
TTTATAGGGGTCCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT	347	0.1288348797231721	No Hit
AGACGAGGGGAGAAATAGTCAAGCTCAGGGCCAAAATCAACCAAATAGAAA	341	0.12660718727839101	No Hit
AGCAGCGGGTGAAATGGGATTTAAGGAGTTCACCTCAATCTCATGTGTATG	325	0.12066667409230815	No Hit
TAACAGGGCAGAAAGTTTTTTTTTTTCTTTTTGCCTTTTTTCTTCAGAGTA	311	0.11546872505448565	No Hit
GCTTCTTTTTTTATTTTCTTTTTATTTTTTAATTTTCTCCAGTCCCTGGGA	305	0.11324103260970458	No Hit
TAAGCTGGGACTGAAGAAGATGGAGGAGAAAAGAAAGAAGCAGTGGCAGCA	288	0.10692923734949153	No Hit
GGTAGGGGTTTTCTATGCTTTATTTTTTAATGTAGCTTACTTCCTGAGATT	288	0.10692923734949153	No Hit
GCAATGGGGAATGGAGAGGATTAATTCCTAAAAGTGGTTATTTTCTCAAGC	281	0.10433026283058029	No Hit
ACACACGGGTCTTTTTCATCTACCTCTTTAAACCAATGTTTTGTGTCATAT	280	0.1039589807564501	No Hit
AATTGGGGACAGTTTTTTTCCAACAGATGCATTGTAGTAATTTTTTTAAAT	279	0.10358769868231991	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	275	0.10210257038579922	No Hit
CGCACTGGGCAATGGGAATACGTTCACTTTTGTTTTTTGAGACAGGGTCTT	274	0.10173128831166903	No Hit
TTAGATGGGGCCTTTTCCTGTTGATCCCAAATTCATGATTTACTTGTTTAC	274	0.10173128831166903	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	55	1.8189894E-12	45.000004	29
CGCGGAT	55	1.8189894E-12	45.000004	4
GGGCGAT	75	0.0	45.000004	7
GCGGGTG	55	1.8189894E-12	45.000004	5
CATACGA	55	1.8189894E-12	45.000004	18
GCATAGG	55	1.8189894E-12	45.000004	2
AAGTCAC	55	1.8189894E-12	45.000004	31
TGATCAT	20	7.0236577E-4	45.0	23
CTTGTGA	35	1.2072996E-7	45.0	30
TCTGCCA	20	7.0236577E-4	45.0	12
GTCATGC	25	3.8828257E-5	45.0	32
CAGGTAC	20	7.0236577E-4	45.0	34
TAATAGA	25	3.8828257E-5	45.0	10
CAGCGCA	25	3.8828257E-5	45.0	29
AACCGCG	25	3.8828257E-5	45.0	1
AGTCCTC	20	7.0236577E-4	45.0	31
TCGTTGA	25	3.8828257E-5	45.0	24
TCGTTCA	20	7.0236577E-4	45.0	16
GGTACCC	25	3.8828257E-5	45.0	9
AGTCCAA	25	3.8828257E-5	45.0	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content