SRR3548589_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266621
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13492	5.060366587778157	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2983	1.1188165973422948	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2419	0.9072803717636645	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1456	0.5460935185150456	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1271	0.476706636011417	TruSeq Adapter, Index 20 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	1042	0.390816927398817	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	924	0.3465593482883944	No Hit
AATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	805	0.3019267049482224	No Hit
GCATACGGGGGAGACAAAGAGAATATGATATAGGAGACACGGATAACTTTT	486	0.18228121565818145	No Hit
AAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	417	0.15640178380547667	No Hit
ATGATAGGGTAAAGTTGATAAAATAATCAAATCCTGAAAGGTAATGAATTA	395	0.1481503707509911	No Hit
TAATTTGGGATTATTTCAGAATCCTCTATTTCTTCCATGTTTGCTTTGCTT	388	0.1455249211427457	No Hit
TTTATAGGGGTCCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT	369	0.13839870077750815	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	367	0.13764857231800945	No Hit
ACGTCTGGGACTTTTCTATTGTTTGATCAAATATCTGGCAGAAGCAATTTA	347	0.13014728772302256	No Hit
GGTAGGGGTTTTCTATGCTTTATTTTTTAATGTAGCTTACTTCCTGAGATT	330	0.12377119581728371	No Hit
AGACGAGGGGAGAAATAGTCAAGCTCAGGGCCAAAATCAACCAAATAGAAA	327	0.12264600312803567	No Hit
AGCAGCGGGTGAAATGGGATTTAAGGAGTTCACCTCAATCTCATGTGTATG	312	0.11702003968179552	No Hit
TAACAGGGCAGAAAGTTTTTTTTTTTCTTTTTGCCTTTTTTCTTCAGAGTA	312	0.11702003968179552	No Hit
GCTTCTTTTTTTATTTTCTTTTTATTTTTTAATTTTCTCCAGTCCCTGGGA	298	0.11176914046530469	No Hit
CGCACTGGGCAATGGGAATACGTTCACTTTTGTTTTTTGAGACAGGGTCTT	286	0.10726836970831255	No Hit
TAAGCTGGGACTGAAGAAGATGGAGGAGAAAAGAAAGAAGCAGTGGCAGCA	277	0.10389279164056844	No Hit
GCAATGGGGAATGGAGAGGATTAATTCCTAAAAGTGGTTATTTTCTCAAGC	277	0.10389279164056844	No Hit
ACACACGGGTCTTTTTCATCTACCTCTTTAAACCAATGTTTTGTGTCATAT	275	0.10314266318106975	No Hit
TACAACGGATGTATGTATGTATGTATCTATCTATCTATCTATCTATCTATC	272	0.10201747049182174	No Hit
GACAATGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	271	0.10164240626207238	No Hit
TTAGATGGGGCCTTTTCCTGTTGATCCCAAATTCATGATTTACTTGTTTAC	268	0.10051721357282435	No Hit
ATGGCAGCTTTTAAAAGATTTTTTTTTTTCTTCAACACCATGAATTCCTTT	268	0.10051721357282435	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	35	1.2072451E-7	45.000004	30
ACAACAG	30	2.1592095E-6	45.000004	1
CTAGAGG	30	2.1592095E-6	45.000004	2
CTCACTA	70	0.0	45.000004	45
ACACGTG	30	2.1592095E-6	45.000004	42
GGTACAA	70	0.0	45.000004	8
ATGATAG	30	2.1592095E-6	45.000004	1
ATCTAGC	35	1.2072451E-7	45.000004	12
CTCAACG	30	2.1592095E-6	45.000004	1
GGCAACC	35	1.2072451E-7	45.000004	8
GAATCCG	30	2.1592095E-6	45.000004	10
GTCTACT	30	2.1592095E-6	45.000004	15
CGACAGT	30	2.1592095E-6	45.000004	26
CGACAGG	30	2.1592095E-6	45.000004	2
TAACGAC	30	2.1592095E-6	45.000004	23
ACCTCTA	30	2.1592095E-6	45.000004	26
AGTATGC	35	1.2072451E-7	45.000004	44
TAGTTCT	35	1.2072451E-7	45.000004	12
TCTACTT	30	2.1592095E-6	45.000004	16
TACGACG	35	1.2072451E-7	45.000004	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content