##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266621
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534511535100386	31.0	31.0	34.0	30.0	34.0
2	31.744374974214335	31.0	31.0	34.0	30.0	34.0
3	31.91833351461438	33.0	31.0	34.0	30.0	34.0
4	35.6618270878888	37.0	35.0	37.0	33.0	37.0
5	35.41710142861965	37.0	35.0	37.0	33.0	37.0
6	32.62241908926904	37.0	35.0	37.0	26.0	37.0
7	34.083320518638814	37.0	35.0	37.0	26.0	37.0
8	35.13395794029728	37.0	35.0	37.0	32.0	37.0
9	36.94661710817977	39.0	37.0	39.0	33.0	39.0
10	36.58156709336474	38.0	35.0	39.0	32.0	39.0
11	36.61013948638704	39.0	35.0	39.0	32.0	39.0
12	36.45938616988159	38.0	35.0	39.0	32.0	39.0
13	36.44333717149062	38.0	35.0	39.0	32.0	39.0
14	37.548936505376545	39.0	36.0	41.0	32.0	41.0
15	37.56989134389264	39.0	36.0	41.0	32.0	41.0
16	37.46732627962538	39.0	36.0	41.0	32.0	41.0
17	37.39699048462049	39.0	36.0	40.0	32.0	41.0
18	37.14626754831765	39.0	36.0	40.0	32.0	41.0
19	36.948912501265845	39.0	35.0	40.0	32.0	41.0
20	36.812156581814634	39.0	35.0	40.0	31.0	41.0
21	36.69172720828442	38.0	35.0	40.0	31.0	41.0
22	36.61027075886746	38.0	35.0	40.0	31.0	41.0
23	36.420026929611694	38.0	35.0	40.0	31.0	41.0
24	36.28052178935643	38.0	35.0	40.0	30.0	41.0
25	36.349946178283034	38.0	35.0	40.0	31.0	41.0
26	36.26547796310118	38.0	35.0	40.0	30.0	41.0
27	36.13075489177522	38.0	35.0	40.0	30.0	41.0
28	36.06902682084307	38.0	35.0	40.0	30.0	41.0
29	35.80723198847803	38.0	34.0	40.0	29.0	41.0
30	35.814812036561264	38.0	34.0	40.0	29.0	41.0
31	35.38177787946186	38.0	34.0	40.0	27.0	41.0
32	35.43829630824278	38.0	34.0	40.0	27.0	41.0
33	35.388589045874106	38.0	34.0	40.0	27.0	41.0
34	35.297497196394886	38.0	34.0	40.0	27.0	41.0
35	35.21987390340596	38.0	34.0	40.0	27.0	41.0
36	35.13803863911695	38.0	34.0	40.0	26.0	41.0
37	35.08970411182915	38.0	34.0	40.0	26.0	41.0
38	34.954058382498005	37.0	34.0	40.0	25.0	41.0
39	34.83696708061255	37.0	33.0	40.0	25.0	41.0
40	34.69394758852454	37.0	33.0	40.0	24.0	41.0
41	34.69154342681184	37.0	33.0	40.0	24.0	41.0
42	34.48102362529583	37.0	33.0	40.0	24.0	41.0
43	34.37358647668413	37.0	33.0	40.0	23.0	41.0
44	34.16933774908953	37.0	33.0	40.0	23.0	41.0
45	33.982585767812736	36.0	33.0	40.0	23.0	41.0
46	33.662014620003674	36.0	33.0	40.0	22.0	41.0
47	33.58490141436721	35.0	33.0	40.0	22.0	41.0
48	33.57995056653452	35.0	33.0	40.0	22.0	41.0
49	33.40373413947138	35.0	32.0	39.0	21.0	41.0
50	33.25130428585895	35.0	32.0	39.0	20.0	41.0
51	32.09214577996482	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	4.0
13	5.0
14	9.0
15	21.0
16	69.0
17	125.0
18	270.0
19	548.0
20	885.0
21	1349.0
22	1774.0
23	2235.0
24	2541.0
25	2914.0
26	3317.0
27	3829.0
28	4633.0
29	5665.0
30	7135.0
31	8912.0
32	11497.0
33	15223.0
34	21797.0
35	30966.0
36	26634.0
37	33498.0
38	42767.0
39	37992.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.552848425292833	36.049673506588	19.545722204927593	24.85175586319157
2	21.519685246098394	38.13465555976461	17.483244005535948	22.862415188601048
3	19.05438806395595	40.72109848811609	18.07772080968866	22.146792638239297
4	18.95199552923438	39.78643842758072	16.615345377895967	24.646220665288933
5	16.140514063033294	41.398839551273156	15.058078695976688	27.40256768971686
6	16.1656433664265	48.022848912876334	16.65810270008739	19.153405020609778
7	74.5657693880077	17.105929390408107	4.495519857775644	3.8327813638085524
8	75.73109394983891	15.213730351322663	3.821154372686322	5.234021326152104
9	69.95923051822625	18.47266344361472	7.569546284801272	3.9985597533577626
10	33.79853799963244	37.18724331541777	13.339159331035441	15.675059353914358
11	24.09562637601689	36.28108813634335	21.634829964631443	17.988455523008316
12	24.299286252770788	32.06536619396071	26.48553564797972	17.149811905288782
13	24.01686288776953	33.72652566752056	24.059245145731207	18.1973662989787
14	20.21671211194917	34.39301480378515	24.426433026655815	20.963840057609865
15	18.829724590336095	36.222578116502454	26.370390929446668	18.577306363714786
16	20.13757355947206	35.20727924657098	25.265451708605095	19.389695485351865
17	19.257672876480097	34.011949546359816	24.8648831112328	21.86549446592729
18	20.576773772508542	35.53996121835865	22.79190311340817	21.091361895724642
19	21.850116832507567	36.7390415608673	24.922267938384447	16.488573668240686
20	22.50647923456892	36.68578244024289	22.344826551546955	18.462911773641235
21	22.264187742150842	35.821634454900405	23.252481987540367	18.66169581540839
22	21.23201098188065	35.69111210294763	23.102831359870375	19.974045555301345
23	21.302898121303272	35.385809819931666	22.057902415788703	21.25338964297636
24	17.84893162954156	36.67715596295866	23.5172773337434	21.95663507375638
25	20.48638329313895	34.5355392110899	23.38863030293938	21.589447192831773
26	21.16712487013401	36.01854317551881	22.241308824136134	20.57302313021105
27	18.903987307826466	35.4818262627475	23.04844704655672	22.565739382869314
28	20.762055502004717	34.19310556932874	23.92572227994044	21.119116648726095
29	20.751178639341987	37.90024041617127	22.204927593850446	19.143653350636296
30	21.708717617892066	34.21973512964095	22.046650488896226	22.024896763570762
31	21.250764193368113	35.44506996823206	21.846366190210073	21.457799648189752
32	22.561238612112327	34.75945255625026	21.233886303029394	21.445422528608024
33	22.11753762831885	33.13880001950334	23.785448258014185	20.958214094163626
34	20.348359656591192	34.364509922324196	23.870587838167285	21.416542582917327
35	21.64795721267267	33.69577040068112	24.09900195408464	20.557270432561577
36	19.28955333600879	34.4207695567866	25.36671905063742	20.922958056567186
37	21.287520487883548	35.292793890953824	21.556066476384082	21.863619144778543
38	20.826191485291858	33.0184044017538	25.6363902318272	20.519013881127144
39	20.510762468072656	33.28094936257834	24.677726060587876	21.530562108761124
40	22.823033444477367	31.27923156840609	24.327791134231738	21.56994385288481
41	21.579695522858287	30.57261055955832	26.538794768604124	21.30889914897926
42	22.719515717066546	32.56795226182484	24.44556130237303	20.266970718735582
43	20.596652176685257	32.20751553703572	24.774867696092954	22.420964590186067
44	20.49163419235544	30.919169907846715	24.771117053795464	23.81807884600238
45	22.983935999039836	30.98255576267436	25.736532381170274	20.29697585711553
46	19.9987997944648	31.093199710450413	27.976040897003614	20.93195959808117
47	20.438375071731034	31.695177799198117	25.679522618248374	22.18692451082248
48	20.714422344826552	30.768394087487483	27.884525224944774	20.632658342741195
49	20.75530434586923	30.90754291672449	26.109346225541124	22.227806511865154
50	20.37873985920089	29.44591761339129	26.52004155711666	23.65530097029116
51	19.552473361063083	29.76659752982698	27.73149901920704	22.949430089902894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15410.0
1	11407.0
2	7404.0
3	4645.0
4	1886.0
5	1425.0
6	964.0
7	992.5
8	1021.0
9	1002.5
10	984.0
11	1044.5
12	1105.0
13	1092.0
14	1079.0
15	1375.0
16	1671.0
17	1671.5
18	1672.0
19	1462.0
20	1252.0
21	1254.0
22	1256.0
23	1444.0
24	1632.0
25	1713.5
26	2522.5
27	3250.0
28	3685.5
29	4121.0
30	3991.5
31	3862.0
32	4589.5
33	5317.0
34	5582.5
35	5848.0
36	6584.5
37	7321.0
38	7145.0
39	6969.0
40	7336.0
41	7703.0
42	9158.0
43	10613.0
44	13431.0
45	16249.0
46	17805.5
47	19362.0
48	19691.0
49	20020.0
50	19565.5
51	19111.0
52	17429.5
53	15748.0
54	15065.5
55	14383.0
56	13880.0
57	13377.0
58	12390.0
59	11403.0
60	10295.0
61	9187.0
62	9068.0
63	8949.0
64	8121.5
65	7294.0
66	6192.0
67	5090.0
68	4586.0
69	4082.0
70	3697.5
71	3313.0
72	2380.5
73	1448.0
74	1374.0
75	988.0
76	676.0
77	749.0
78	822.0
79	508.0
80	194.0
81	182.5
82	171.0
83	127.5
84	84.0
85	115.0
86	146.0
87	100.0
88	54.0
89	33.5
90	13.0
91	10.5
92	8.0
93	5.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	266621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.441199305381048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.39199606363785	22.06052786539695
2	8.044940134492373	4.415256112609285
3	2.867530479470778	2.3606542620423747
4	1.4911705210212673	1.6367802986261397
5	0.9635886501558144	1.3221014098664396
6	0.6779290361379914	1.1161911477340496
7	0.4934120605762397	0.9477873085765938
8	0.4018369690011481	0.8821510683704583
9	0.3307637635995845	0.8168898923940725
>10	3.0424799081515497	18.326013329782725
>50	0.7626701656552404	14.868671259953267
>100	0.5207479088076103	22.098409352601635
>500	0.002733584823137062	0.6484860532366168
>1k	0.006833962057842655	3.4397140510312387
>5k	0.0	0.0
>10k+	0.001366792411568531	5.060366587778157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13492	5.060366587778157	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2983	1.1188165973422948	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2419	0.9072803717636645	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1456	0.5460935185150456	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1271	0.476706636011417	TruSeq Adapter, Index 20 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	1042	0.390816927398817	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	924	0.3465593482883944	No Hit
AATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	805	0.3019267049482224	No Hit
GCATACGGGGGAGACAAAGAGAATATGATATAGGAGACACGGATAACTTTT	486	0.18228121565818145	No Hit
AAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	417	0.15640178380547667	No Hit
ATGATAGGGTAAAGTTGATAAAATAATCAAATCCTGAAAGGTAATGAATTA	395	0.1481503707509911	No Hit
TAATTTGGGATTATTTCAGAATCCTCTATTTCTTCCATGTTTGCTTTGCTT	388	0.1455249211427457	No Hit
TTTATAGGGGTCCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT	369	0.13839870077750815	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	367	0.13764857231800945	No Hit
ACGTCTGGGACTTTTCTATTGTTTGATCAAATATCTGGCAGAAGCAATTTA	347	0.13014728772302256	No Hit
GGTAGGGGTTTTCTATGCTTTATTTTTTAATGTAGCTTACTTCCTGAGATT	330	0.12377119581728371	No Hit
AGACGAGGGGAGAAATAGTCAAGCTCAGGGCCAAAATCAACCAAATAGAAA	327	0.12264600312803567	No Hit
AGCAGCGGGTGAAATGGGATTTAAGGAGTTCACCTCAATCTCATGTGTATG	312	0.11702003968179552	No Hit
TAACAGGGCAGAAAGTTTTTTTTTTTCTTTTTGCCTTTTTTCTTCAGAGTA	312	0.11702003968179552	No Hit
GCTTCTTTTTTTATTTTCTTTTTATTTTTTAATTTTCTCCAGTCCCTGGGA	298	0.11176914046530469	No Hit
CGCACTGGGCAATGGGAATACGTTCACTTTTGTTTTTTGAGACAGGGTCTT	286	0.10726836970831255	No Hit
TAAGCTGGGACTGAAGAAGATGGAGGAGAAAAGAAAGAAGCAGTGGCAGCA	277	0.10389279164056844	No Hit
GCAATGGGGAATGGAGAGGATTAATTCCTAAAAGTGGTTATTTTCTCAAGC	277	0.10389279164056844	No Hit
ACACACGGGTCTTTTTCATCTACCTCTTTAAACCAATGTTTTGTGTCATAT	275	0.10314266318106975	No Hit
TACAACGGATGTATGTATGTATGTATCTATCTATCTATCTATCTATCTATC	272	0.10201747049182174	No Hit
GACAATGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTA	271	0.10164240626207238	No Hit
TTAGATGGGGCCTTTTCCTGTTGATCCCAAATTCATGATTTACTTGTTTAC	268	0.10051721357282435	No Hit
ATGGCAGCTTTTAAAAGATTTTTTTTTTTCTTCAACACCATGAATTCCTTT	268	0.10051721357282435	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011251926892480338	0.0	0.0	0.5877256480172229	0.0
2	0.0011251926892480338	0.0	0.0	0.7370012114574621	0.0
3	0.0011251926892480338	0.0	0.0	0.9365353816841134	0.0
4	0.0011251926892480338	0.0	0.0	2.0456003090529253	0.0
5	0.0011251926892480338	0.0	0.0	2.226756332021859	0.0
6	0.0011251926892480338	0.0	0.0	3.1434133095292567	0.0
7	0.0011251926892480338	0.0	0.0	3.887165677122207	0.0
8	0.0011251926892480338	0.0	0.0	4.4621391413279525	0.0
9	0.0011251926892480338	0.0	0.0	5.62483825355092	0.0
10	0.0011251926892480338	0.0	0.0	6.846047385614787	0.0
11	0.0011251926892480338	0.0	0.0	8.128016922898047	0.0
12	0.0011251926892480338	0.0	0.0	8.662483450290862	0.0
13	0.0011251926892480338	0.0	0.0	8.878520446626485	0.0
14	0.0011251926892480338	0.0	0.0	9.117061296747067	0.0
15	0.0011251926892480338	0.0	0.0	9.303093154702744	0.0
16	0.0011251926892480338	0.0	0.0	9.7002861740073	0.0
17	0.0011251926892480338	0.0	0.0	10.158989726990747	0.0
18	0.0011251926892480338	0.0	0.0	11.055018171861931	0.0
19	0.0011251926892480338	0.0	0.0	11.299184985428754	0.0
20	0.0011251926892480338	0.0	0.0	11.612363617269457	0.0
21	0.0011251926892480338	0.0	0.0	11.871908064256004	0.0
22	0.0011251926892480338	0.0	0.0	12.128451997404555	0.0
23	0.0011251926892480338	0.0	0.0	12.405249398959572	0.0
24	0.0011251926892480338	0.0	0.0	12.579654265793017	0.0
25	0.0011251926892480338	0.0	0.0	12.758934967613204	0.0
26	0.0011251926892480338	0.0	0.0	12.91533675141868	0.0
27	0.0011251926892480338	0.0	0.0	13.122747270470068	0.0
28	0.0011251926892480338	0.0	0.0	13.297527201533262	0.0
29	0.0011251926892480338	0.0	0.0	13.468181426069215	0.0
30	0.0011251926892480338	0.0	0.0	13.728100937285511	0.0
31	0.0011251926892480338	0.0	0.0	13.9058813821867	0.0
32	0.0011251926892480338	0.0	0.0	14.096038946669617	0.0
33	0.0011251926892480338	0.0	0.0	14.284696254233538	0.0
34	0.0011251926892480338	0.0	0.0	14.46660240566197	0.0
35	0.0011251926892480338	0.0	0.0	14.66613657588862	0.0
36	0.0011251926892480338	0.0	0.0	14.851043241155049	0.0
37	0.0015002569189973784	0.0	0.0	15.019447080312503	0.0
38	0.0015002569189973784	0.0	0.0	15.220481507458151	0.0
39	0.0015002569189973784	0.0	0.0	15.430142411888037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	35	1.2072451E-7	45.000004	30
ACAACAG	30	2.1592095E-6	45.000004	1
CTAGAGG	30	2.1592095E-6	45.000004	2
CTCACTA	70	0.0	45.000004	45
ACACGTG	30	2.1592095E-6	45.000004	42
GGTACAA	70	0.0	45.000004	8
ATGATAG	30	2.1592095E-6	45.000004	1
ATCTAGC	35	1.2072451E-7	45.000004	12
CTCAACG	30	2.1592095E-6	45.000004	1
GGCAACC	35	1.2072451E-7	45.000004	8
GAATCCG	30	2.1592095E-6	45.000004	10
GTCTACT	30	2.1592095E-6	45.000004	15
CGACAGT	30	2.1592095E-6	45.000004	26
CGACAGG	30	2.1592095E-6	45.000004	2
TAACGAC	30	2.1592095E-6	45.000004	23
ACCTCTA	30	2.1592095E-6	45.000004	26
AGTATGC	35	1.2072451E-7	45.000004	44
TAGTTCT	35	1.2072451E-7	45.000004	12
TCTACTT	30	2.1592095E-6	45.000004	16
TACGACG	35	1.2072451E-7	45.000004	14
>>END_MODULE