##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129174
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78297676000333	31.0	31.0	34.0	30.0	34.0
2	31.986553002460205	33.0	31.0	34.0	30.0	34.0
3	32.15533655574783	34.0	31.0	34.0	30.0	34.0
4	35.863594981818565	37.0	35.0	37.0	35.0	37.0
5	35.61412235846734	37.0	35.0	37.0	33.0	37.0
6	34.17152272369006	37.0	35.0	37.0	32.0	37.0
7	35.00394978984638	37.0	35.0	37.0	32.0	37.0
8	35.72435072008388	37.0	35.0	37.0	33.0	37.0
9	37.44813465418084	39.0	37.0	39.0	35.0	39.0
10	36.85343091498741	39.0	37.0	39.0	32.0	39.0
11	36.88804825474196	39.0	37.0	39.0	33.0	39.0
12	36.82873764362268	39.0	37.0	39.0	33.0	39.0
13	36.811633105261016	39.0	37.0	39.0	33.0	39.0
14	37.986713296622135	40.0	37.0	41.0	33.0	41.0
15	37.9777545356163	40.0	37.0	41.0	33.0	41.0
16	37.89862855503226	40.0	37.0	41.0	33.0	41.0
17	37.887358369923504	40.0	37.0	41.0	33.0	41.0
18	37.79750596453691	39.0	37.0	41.0	33.0	41.0
19	37.80917909905825	40.0	36.0	41.0	33.0	41.0
20	37.68604838581122	39.0	36.0	41.0	33.0	41.0
21	37.64562680330932	39.0	36.0	41.0	33.0	41.0
22	37.5781305626945	39.0	36.0	41.0	32.0	41.0
23	37.45333491561088	39.0	36.0	41.0	32.0	41.0
24	37.27072886906712	39.0	36.0	41.0	32.0	41.0
25	37.29133508210426	39.0	36.0	41.0	32.0	41.0
26	37.2620039072809	39.0	36.0	41.0	32.0	41.0
27	37.21679563999879	39.0	35.0	41.0	32.0	41.0
28	37.11369018415231	39.0	35.0	41.0	31.0	41.0
29	36.96652508825035	39.0	35.0	40.0	31.0	41.0
30	36.96260895132194	39.0	35.0	40.0	31.0	41.0
31	36.92516033844208	39.0	35.0	40.0	31.0	41.0
32	36.844516434136814	39.0	35.0	40.0	31.0	41.0
33	36.82023054020018	39.0	35.0	40.0	31.0	41.0
34	36.686421224718245	39.0	35.0	40.0	31.0	41.0
35	36.603612906425404	39.0	35.0	40.0	30.0	41.0
36	36.535111506286896	39.0	35.0	40.0	30.0	41.0
37	36.51991544261558	39.0	35.0	40.0	30.0	41.0
38	36.428865701831604	39.0	35.0	40.0	30.0	41.0
39	36.399700134788795	39.0	35.0	40.0	30.0	41.0
40	36.25448336571689	38.0	35.0	40.0	30.0	41.0
41	36.15054012933348	38.0	35.0	40.0	30.0	41.0
42	36.08246381868516	38.0	35.0	40.0	30.0	41.0
43	36.0027701665111	38.0	35.0	40.0	30.0	41.0
44	35.873316247097435	38.0	35.0	40.0	29.0	41.0
45	35.736790786893785	38.0	35.0	40.0	29.0	41.0
46	35.566853292760904	38.0	34.0	40.0	28.0	41.0
47	35.452397947526244	38.0	34.0	40.0	28.0	41.0
48	35.33276979455779	38.0	34.0	40.0	28.0	41.0
49	35.15599721566384	37.0	34.0	40.0	27.0	41.0
50	35.071161751864636	37.0	34.0	40.0	27.0	41.0
51	33.89060764771417	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	13.0
11	15.0
12	13.0
13	24.0
14	35.0
15	88.0
16	127.0
17	275.0
18	495.0
19	962.0
20	1583.0
21	2454.0
22	3334.0
23	4326.0
24	5455.0
25	6786.0
26	8211.0
27	9904.0
28	12687.0
29	16542.0
30	21479.0
31	27828.0
32	37193.0
33	50858.0
34	75998.0
35	100711.0
36	114979.0
37	158642.0
38	226826.0
39	241287.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.12322812958853	32.45124312107788	20.14047436444693	26.285054384886653
2	23.60389098580024	30.16257901793701	20.135426426750882	26.09810356951187
3	20.751009144737658	32.00578475947905	20.523409146863106	26.719796948920184
4	20.663157316764288	32.900421015715914	18.689325117298132	27.747096550221666
5	18.560558425893618	35.55395359793973	16.477619923944406	29.40786805222224
6	18.32534224131976	42.29764411862122	17.36348870944602	22.013524930612995
7	86.38385226723251	6.890612075729692	3.683666113459928	3.041869543577872
8	87.44294501998806	6.434880718117846	3.154783939410578	2.9673903224835145
9	80.57252469504257	9.695582788835026	5.545026718645665	4.18686579747674
10	35.161277181373286	37.33800105209649	12.277115838657284	15.22360592787294
11	25.91407524438218	29.955436451778027	24.291561796499035	19.838926507340766
12	25.372971747489757	26.964666207333853	28.17767677966372	19.484685265512667
13	27.04773577854255	24.96665704311293	27.275867138279843	20.709740040064684
14	21.772729446480348	29.411144783709155	27.08484254862404	21.73128322118646
15	20.092208995247855	31.497094336213905	27.88578199639737	20.524914672140877
16	22.55967636520147	29.45675334359452	27.139130018934193	20.844440272269818
17	22.30320570611792	28.272082070610903	27.562979664781512	21.861732558489656
18	21.956226409747302	29.61456781682894	26.739988699704387	21.689217073719373
19	22.271855356216136	31.83893713457802	24.846303581201834	21.04290392800401
20	23.246638693416603	30.30817216832835	26.109882090802657	20.335307047452385
21	25.32665470512073	29.4098163790523	25.86448147052624	19.399047445300724
22	22.862995428516776	28.564065414187716	25.176544978896075	23.396394178399433
23	21.747135516758267	30.201014192675352	25.693294390412813	22.35855590015356
24	21.061501593199985	30.901260567459044	25.770607541441798	22.266630297899173
25	22.55604539247273	30.56933652386612	24.58894731901372	22.285670764647435
26	22.938006011473874	30.228733569848405	24.629153699961208	22.204106718716513
27	21.623948124912545	28.9521366946104	26.259106213922745	23.16480896655431
28	20.563704088121053	30.719711931022147	28.141721293618165	20.57486268723864
29	22.749461110510868	29.823127347955232	24.954612840890775	22.472798700643125
30	22.833504845134588	29.7615779321876	26.00865765595028	21.396259566727537
31	22.04620368517164	29.71729777695909	23.602208339901555	24.63429019796772
32	22.251132243569195	29.265551633317806	26.638232902989266	21.845083220123737
33	21.618545945974667	29.361019647990478	25.812585128598425	23.207849277436427
34	21.059907507611758	30.469086252428767	26.754158349377512	21.716847890581963
35	22.484134420381626	28.502958799972365	26.539045355277395	22.47386142436861
36	22.881770214333663	31.280475816836024	25.18035307224573	20.657400896584583
37	21.748109680173293	30.119273026123523	24.92264256881579	23.209974724887395
38	21.2961864159111	29.717474897579997	26.2270473815373	22.759291304971597
39	21.132881203428347	28.166783861477505	25.360838984957145	25.339495950137003
40	21.466842134161784	28.71196113265095	26.740520061567132	23.08067667162014
41	20.368605724184228	27.917486587540978	27.997456547883676	23.71645114039112
42	21.219404626744858	29.310983072582257	25.24943011440221	24.220182186270673
43	21.051582838428796	28.2473737439934	26.44942232109489	24.251621096482918
44	21.618988747526956	27.91031320239396	26.478470102924796	23.99222794715429
45	22.758494262177486	27.145772042218468	27.222642391695167	22.873091303908875
46	22.617506247929903	27.370006748295655	27.77295616087512	22.23953084289932
47	21.00181194395195	29.056637860949685	27.47468503525586	22.466865159842506
48	20.74498704362658	28.502958799972365	27.539953984062688	23.212100172338364
49	20.229211795524872	28.749422143974268	27.971331256298853	23.05003480420201
50	21.217810541156634	27.039145428428213	28.11302775303009	23.630016277385064
51	21.561778786971715	26.733346676420112	27.910933124567162	23.793941412041015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18522.0
1	13540.5
2	8559.0
3	5478.0
4	2397.0
5	2034.0
6	1671.0
7	1706.0
8	1741.0
9	1791.5
10	1842.0
11	2111.0
12	2380.0
13	2619.0
14	2858.0
15	2532.0
16	2206.0
17	2557.5
18	2909.0
19	3422.0
20	3935.0
21	4177.5
22	4420.0
23	6107.0
24	7794.0
25	7424.0
26	10047.5
27	13041.0
28	15020.5
29	17000.0
30	22385.0
31	27770.0
32	28566.5
33	29363.0
34	34993.0
35	40623.0
36	41138.0
37	41653.0
38	43463.0
39	45273.0
40	48714.5
41	52156.0
42	54511.5
43	56867.0
44	61627.5
45	66388.0
46	71280.0
47	76172.0
48	76541.5
49	76911.0
50	77772.0
51	78633.0
52	75583.5
53	72534.0
54	67227.0
55	61920.0
56	61405.5
57	60891.0
58	56476.5
59	52062.0
60	48565.5
61	45069.0
62	41250.0
63	37431.0
64	33376.5
65	29322.0
66	26052.5
67	22783.0
68	19783.0
69	16783.0
70	15752.5
71	14722.0
72	12341.5
73	9961.0
74	7799.0
75	4834.5
76	4032.0
77	3566.5
78	3101.0
79	2267.0
80	1433.0
81	1031.0
82	629.0
83	463.0
84	297.0
85	274.0
86	251.0
87	174.5
88	98.0
89	63.0
90	28.0
91	23.0
92	18.0
93	17.5
94	17.0
95	13.5
96	10.0
97	7.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1129174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.352139864668814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0957054889377	14.919668755872378
2	9.280417287028529	3.591918666821329
3	3.5289120630601243	2.048759994433695
4	1.7652565149044612	1.3664596389379586
5	1.0701781113191722	1.0355118245177868
6	0.7244697551995691	0.8412024018206664
7	0.501265438140467	0.6790391207753168
8	0.38039076348740014	0.5889100206589059
9	0.29808961214621793	0.519180467981264
>10	3.0092026938749874	13.560032197070662
>50	1.0896679601540684	15.332158462108572
>100	1.2461379079969277	42.0404561177175
>500	0.007027093466148088	0.8294440436234898
>1k	0.0028108373864592353	1.1922634842816169
>5k	0.0	0.0
>10k+	4.6847289774320583E-4	1.4549948033788813
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16049	1.4213044225247835	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3286	0.291009180161782	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2627	0.23264793557060295	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	2146	0.19005042624077423	TruSeq Adapter, Index 13 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2138	0.18934194375711805	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1726	0.152855095848824	No Hit
ATAAGCAAACCAATTTGAAATAAAATATGAACATGTGCCATTGTATTATAA	1228	0.1087520612412259	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.856031045702434E-5	0.0	0.0	0.2237033442144435	0.0
2	8.856031045702434E-5	0.0	0.0	0.2964999194101175	0.0
3	8.856031045702434E-5	0.0	0.0	0.39028528818410624	0.0
4	8.856031045702434E-5	0.0	0.0	0.8160832608614793	0.0
5	8.856031045702434E-5	0.0	0.0	0.8850717427075012	0.0
6	8.856031045702434E-5	0.0	0.0	1.3259249681625684	0.0
7	8.856031045702434E-5	0.0	0.0	1.6619227860365187	0.0
8	8.856031045702434E-5	0.0	0.0	1.9924298646621335	0.0
9	8.856031045702434E-5	0.0	0.0	2.4691500158522954	0.0
10	8.856031045702434E-5	0.0	0.0	3.2309458064036187	0.0
11	8.856031045702434E-5	0.0	0.0	3.8464399640799383	0.0
12	8.856031045702434E-5	0.0	0.0	4.141700039143657	0.0
13	8.856031045702434E-5	0.0	0.0	4.26780992123446	0.0
14	8.856031045702434E-5	0.0	0.0	4.405786884926504	0.0
15	8.856031045702434E-5	0.0	0.0	4.53269380981142	0.0
16	8.856031045702434E-5	0.0	0.0	4.792175519450501	0.0
17	8.856031045702434E-5	0.0	0.0	5.095937384318095	0.0
18	8.856031045702434E-5	0.0	0.0	5.539181738155501	0.0
19	8.856031045702434E-5	0.0	0.0	5.713556989445382	0.0
20	8.856031045702434E-5	0.0	0.0	5.958160566927684	0.0
21	8.856031045702434E-5	0.0	0.0	6.160875117563812	0.0
22	8.856031045702434E-5	0.0	0.0	6.419205543166952	0.0
23	8.856031045702434E-5	0.0	0.0	6.688783128198134	0.0
24	8.856031045702434E-5	0.0	0.0	6.883438690582674	0.0
25	8.856031045702434E-5	0.0	0.0	7.048692229895481	0.0
26	8.856031045702434E-5	0.0	0.0	7.203938454126645	0.0
27	8.856031045702434E-5	0.0	0.0	7.37344288834139	0.0
28	8.856031045702434E-5	0.0	0.0	7.550652069565895	0.0
29	8.856031045702434E-5	0.0	0.0	7.721750589368866	0.0
30	8.856031045702434E-5	0.0	0.0	7.941114478370915	0.0
31	8.856031045702434E-5	0.0	0.0	8.116109651833996	0.0
32	8.856031045702434E-5	0.0	0.0	8.30137782131009	0.0
33	8.856031045702434E-5	0.0	0.0	8.497716029593313	0.0
34	8.856031045702434E-5	0.0	0.0	8.677759140752444	0.0
35	8.856031045702434E-5	0.0	0.0	8.901019683414603	0.0
36	8.856031045702434E-5	0.0	0.0	9.094081160210916	0.0
37	8.856031045702434E-5	0.0	0.0	9.29360753967059	0.0
38	8.856031045702434E-5	0.0	0.0	9.5112002224635	0.0
39	8.856031045702434E-5	0.0	0.0	9.730741232086464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCG	35	1.2119017E-7	45.000004	13
CGTATAG	50	2.1827873E-11	45.000004	1
CGTCTAA	35	1.2119017E-7	45.000004	35
CCTCGCG	25	3.8907616E-5	45.000004	1
TATCTCG	25	3.8907616E-5	45.000004	1
CGTAGGA	25	3.8907616E-5	45.000004	26
CTCGCAC	25	3.8907616E-5	45.000004	38
TATAACG	25	3.8907616E-5	45.000004	1
TATCGGA	25	3.8907616E-5	45.000004	14
TCGCGAC	35	1.2119017E-7	45.000004	36
ATTTCGA	35	1.2119017E-7	45.000004	14
CCGATTA	20	7.0332346E-4	45.0	19
CGCGATT	20	7.0332346E-4	45.0	29
ATCCGTA	20	7.0332346E-4	45.0	11
GTAATCG	45	3.8562575E-10	45.0	30
CTAACGG	135	0.0	45.0	2
GCATTAG	40	6.8157533E-9	45.0	1
CCGTAAC	20	7.0332346E-4	45.0	13
TACCTCG	20	7.0332346E-4	45.0	29
CGAATGT	20	7.0332346E-4	45.0	40
>>END_MODULE