FastQCFastQC Report
Sat 18 Jun 2016
SRR3548585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115696
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157031.4074622477807575No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31240.2800045890636876No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24840.222641292968694No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG21310.19100184996629904No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT19600.17567509429091796TruSeq Adapter, Index 13 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.15774906426123245No Hit
ATAAGCAAACCAATTTGAAATAAAATATGAACATGTGCCATTGTATTATAA12950.11607104444221365No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA207.033197E-445.00000423
TCCGCAA207.033197E-445.00000419
GATCGAC207.033197E-445.00000436
ACGTTGT207.033197E-445.00000440
CCGATAT406.8139343E-945.00000421
CCTAGCG406.8139343E-945.0000041
TAAACCG406.8139343E-945.0000041
ATCACGC207.033197E-445.00000442
AACGGCC207.033197E-445.00000433
TAACACG551.8189894E-1245.0000041
CGAATTG207.033197E-445.0000042
CGTCGGA207.033197E-445.00000414
TTCGGAT406.8139343E-945.00000417
TAGGCGT207.033197E-445.00000433
ATACGTT207.033197E-445.00000417
CACGTAT207.033197E-445.00000445
ATCGGCG207.033197E-445.00000438
CCTATCG207.033197E-445.00000412
GATTCGC406.8139343E-945.00000423
TATACGA406.8139343E-945.00000423