##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1115696
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763069868494643	31.0	31.0	34.0	30.0	34.0
2	31.976418307495948	33.0	31.0	34.0	30.0	34.0
3	32.14198670605613	34.0	31.0	34.0	30.0	34.0
4	35.862170340307756	37.0	35.0	37.0	35.0	37.0
5	35.6277794309561	37.0	35.0	37.0	33.0	37.0
6	32.67240897161951	37.0	35.0	37.0	26.0	37.0
7	34.2510271615207	37.0	35.0	37.0	27.0	37.0
8	35.421637256026735	37.0	35.0	37.0	32.0	37.0
9	37.31790649065695	39.0	37.0	39.0	34.0	39.0
10	36.8132833675123	39.0	37.0	39.0	32.0	39.0
11	36.89211935867835	39.0	37.0	39.0	33.0	39.0
12	36.846538842121866	39.0	37.0	39.0	33.0	39.0
13	36.84633807058554	39.0	37.0	39.0	33.0	39.0
14	38.00018732701381	40.0	37.0	41.0	33.0	41.0
15	38.0253608509845	40.0	37.0	41.0	33.0	41.0
16	37.975490635441915	40.0	37.0	41.0	33.0	41.0
17	37.97060937746483	40.0	37.0	41.0	33.0	41.0
18	37.88781173366222	40.0	37.0	41.0	33.0	41.0
19	37.89548407451492	40.0	37.0	41.0	33.0	41.0
20	37.76791437811016	39.0	36.0	41.0	33.0	41.0
21	37.71715682408111	39.0	36.0	41.0	33.0	41.0
22	37.614053469762375	39.0	36.0	41.0	33.0	41.0
23	37.493304627783914	39.0	36.0	41.0	32.0	41.0
24	37.3749399477994	39.0	36.0	41.0	32.0	41.0
25	37.36432146391132	39.0	36.0	41.0	32.0	41.0
26	37.336709999856595	39.0	36.0	41.0	32.0	41.0
27	37.26619616813182	39.0	36.0	41.0	32.0	41.0
28	37.198235003083276	39.0	35.0	41.0	32.0	41.0
29	37.02233314451249	39.0	35.0	41.0	31.0	41.0
30	37.019907752649466	39.0	35.0	40.0	31.0	41.0
31	36.69415414234702	39.0	35.0	40.0	31.0	41.0
32	36.79090451162324	39.0	35.0	40.0	31.0	41.0
33	36.81422358778735	39.0	35.0	40.0	31.0	41.0
34	36.71009576085242	39.0	35.0	40.0	31.0	41.0
35	36.70100995253187	39.0	35.0	40.0	31.0	41.0
36	36.620849227746625	39.0	35.0	40.0	31.0	41.0
37	36.584333008274655	39.0	35.0	40.0	31.0	41.0
38	36.48234286042076	39.0	35.0	40.0	30.0	41.0
39	36.44266717815606	39.0	35.0	40.0	30.0	41.0
40	36.31517545998193	39.0	35.0	40.0	30.0	41.0
41	36.265299866630336	38.0	35.0	40.0	30.0	41.0
42	36.18886775609126	38.0	35.0	40.0	30.0	41.0
43	36.118885431156876	38.0	35.0	40.0	30.0	41.0
44	35.94473315311698	38.0	35.0	40.0	30.0	41.0
45	35.79742151984053	38.0	35.0	40.0	29.0	41.0
46	35.53712391188998	38.0	34.0	40.0	28.0	41.0
47	35.48898624714976	38.0	34.0	40.0	28.0	41.0
48	35.47304014713686	38.0	34.0	40.0	28.0	41.0
49	35.33422993360198	38.0	34.0	40.0	28.0	41.0
50	35.21675617730995	37.0	34.0	40.0	27.0	41.0
51	34.11915163270282	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	5.0
11	11.0
12	13.0
13	28.0
14	39.0
15	89.0
16	140.0
17	253.0
18	525.0
19	913.0
20	1546.0
21	2404.0
22	3291.0
23	4274.0
24	5245.0
25	6555.0
26	7728.0
27	9592.0
28	12146.0
29	15597.0
30	21014.0
31	27537.0
32	36408.0
33	51176.0
34	76058.0
35	100870.0
36	117288.0
37	162049.0
38	220972.0
39	231891.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.166428848001605	32.499802813669675	20.11381236465847	26.219955973670245
2	23.74024823966385	30.009608352095913	20.09346632057478	26.156677087665457
3	20.79849708164231	32.02745192238746	20.55739197774304	26.61665901822719
4	20.72786852332535	32.89032137786637	18.650241642884797	27.731568455923476
5	18.61931924108359	35.42156644820811	16.453227402446544	29.505886908261747
6	17.543130028251426	44.765599231331834	16.57700663980152	21.11426410061522
7	86.44200570764797	6.858230198907229	3.6773458002896846	3.0224182931551247
8	87.31661671279632	6.472372402518249	3.1087321277480604	3.1022787569373733
9	80.55581448710043	9.73885359452754	5.5331380591128765	4.172193859259153
10	35.1914858527771	37.31061149273637	12.331136797120362	15.166765857366164
11	25.979209390371572	29.934050135520785	24.383344566978817	19.703395907128822
12	25.465180479270337	26.86574120548967	28.123341842222256	19.54573647301774
13	27.041237039480286	24.981984339820166	27.340332850525588	20.636445770173953
14	21.815082244625778	29.32501326526222	27.036845162123015	21.823059327988986
15	20.123582051024652	31.494062898854168	27.920777702886806	20.46157734723437
16	22.514914456984698	29.465642970845106	27.19575941833618	20.82368315383402
17	22.29549984942135	28.353063917052673	27.506776039351223	21.844660194174757
18	21.968529061679885	29.529997418651675	26.807391977743038	21.6940815419254
19	22.230697250864033	31.846040498487042	24.89226455952159	21.030997691127332
20	23.32077913696921	30.138406877859204	26.17200384334084	20.36881014183075
21	25.365153231704696	29.389636603519236	25.903920064246893	19.341290100529175
22	22.757991424187235	28.603759447017822	25.39168375614863	23.246565372646312
23	21.872176650270326	30.22167328734709	25.70225222641293	22.203897835969656
24	21.065505298934475	30.856882161448993	25.85435459121481	22.223257948401713
25	22.43227545854785	30.725036210580658	24.593975419827625	22.24871291104387
26	22.946304369649077	30.231442973713268	24.666755101748148	22.155497554889504
27	21.636270095079663	29.014444794997917	26.218790781718315	23.130494328204097
28	20.58141285798282	30.7086338931035	28.205353429608067	20.504599819305618
29	22.72904088568929	29.761332836184767	25.002778534654603	22.50684774347134
30	22.852013451692933	29.7878633606287	26.07600995253187	21.284113235146492
31	22.10270539645208	29.673136768438713	23.679030847112475	24.545126987996728
32	22.242438800533478	29.373144655891924	26.68155124693465	21.702865296639946
33	21.599073582768067	29.407562633548924	25.84808048070442	23.14528330297859
34	21.125916020134518	30.48904002523985	26.737838981227863	21.64720497339777
35	22.4777179446731	28.427456941675867	26.659681490298432	22.435143623352598
36	22.92864723006984	31.376916292610176	25.143318610087334	20.55111786723265
37	21.640572342286788	30.00109348783181	25.14699344624342	23.21134072363798
38	21.219579527039624	29.783650743571727	26.326615852346873	22.670153877041773
39	21.15307395562949	28.166543574593796	25.515283733203308	25.165098736573405
40	21.57084008547131	28.71508009350217	26.73506044657326	22.979019374453255
41	20.31637650399392	28.018295306248298	28.024300526308245	23.64102766344954
42	21.145724283317318	29.38838178141716	25.2650363539889	24.20085758127662
43	21.069269765240712	28.284407222038976	26.47943525834994	24.166887754370368
44	21.63582194432892	27.956002351895137	26.47181669558733	23.93635900818861
45	22.732894982145673	27.012286500982345	27.364353730765366	22.89046478610661
46	22.48757726118943	27.498798955988015	27.74124851213951	22.272375270683053
47	20.94405644548336	29.100758629590857	27.576329035866397	22.378855889059384
48	20.797959300741418	28.62527068305345	27.54451033256371	23.032259683641424
49	20.16759045474753	28.89846338070586	27.99301960390644	22.94092656064017
50	21.1838170971304	27.19333940428217	28.17828512426324	23.44455837432419
51	21.525128708895615	26.786060002007716	27.938434842466048	23.75037644663062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18234.0
1	13289.0
2	8344.0
3	5400.0
4	2456.0
5	2014.0
6	1572.0
7	1591.5
8	1611.0
9	1698.0
10	1785.0
11	2070.5
12	2356.0
13	2573.0
14	2790.0
15	2564.5
16	2339.0
17	2619.0
18	2899.0
19	3436.0
20	3973.0
21	4248.5
22	4524.0
23	6356.5
24	8189.0
25	7709.0
26	10031.5
27	12834.0
28	15016.0
29	17198.0
30	22385.0
31	27572.0
32	28693.0
33	29814.0
34	35222.5
35	40631.0
36	40998.0
37	41365.0
38	43040.0
39	44715.0
40	48347.0
41	51979.0
42	54121.0
43	56263.0
44	60835.0
45	65407.0
46	70497.5
47	75588.0
48	75560.0
49	75532.0
50	76514.0
51	77496.0
52	74220.5
53	70945.0
54	66019.5
55	61094.0
56	60320.0
57	59546.0
58	55444.5
59	51343.0
60	47899.0
61	44455.0
62	40628.0
63	36801.0
64	32802.5
65	28804.0
66	25475.5
67	22147.0
68	19415.0
69	16683.0
70	15533.0
71	14383.0
72	12081.0
73	9779.0
74	7543.0
75	4680.0
76	4053.0
77	3545.5
78	3038.0
79	2224.5
80	1411.0
81	999.5
82	588.0
83	423.0
84	258.0
85	236.5
86	215.0
87	152.0
88	89.0
89	58.0
90	27.0
91	19.0
92	11.0
93	13.5
94	16.0
95	9.5
96	3.0
97	2.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1115696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.4629970884548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6743943424908	14.728505167584787
2	9.338802332979187	3.6352216523285756
3	3.5604744341437304	2.0789251063577154
4	1.861784115574253	1.449435952830123
5	1.2529715870987401	1.2193291175809684
6	0.887042947731314	1.0358708585417356
7	0.6402959283162889	0.8723454451998576
8	0.5080197493682437	0.7910069522265332
9	0.41378303699175906	0.7248112239800333
>10	3.580955603086494	15.426752740986686
>50	1.1120504188490568	15.697136182346949
>100	1.1586968290273136	38.886724939233616
>500	0.007463425629805563	0.8703835451874923
>1k	0.0027987846111770863	1.1579088072724812
>5k	0.0	0.0
>10k+	4.664641018628477E-4	1.425642308342463
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15703	1.4074622477807575	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3124	0.2800045890636876	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2484	0.222641292968694	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2131	0.19100184996629904	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	1960	0.17567509429091796	TruSeq Adapter, Index 13 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1760	0.15774906426123245	No Hit
ATAAGCAAACCAATTTGAAATAAAATATGAACATGTGCCATTGTATTATAA	1295	0.11607104444221365	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6889045044528256E-4	0.0	0.0	0.20928640059657827	0.0
2	2.6889045044528256E-4	0.0	0.0	0.282603863417992	0.0
3	2.6889045044528256E-4	0.0	0.0	0.36739398545840446	0.0
4	2.6889045044528256E-4	0.0	0.0	0.7636488792646026	0.0
5	3.585206005937101E-4	0.0	0.0	0.8298855602242905	0.0
6	3.585206005937101E-4	0.0	0.0	1.2489961423183376	0.0
7	3.585206005937101E-4	0.0	0.0	1.564583900990951	0.0
8	3.585206005937101E-4	0.0	0.0	1.8904791269306334	0.0
9	3.585206005937101E-4	0.0	0.0	2.3347757812163885	0.0
10	3.585206005937101E-4	0.0	0.0	3.0730593279889864	0.0
11	3.585206005937101E-4	0.0	0.0	3.6542212215513903	0.0
12	3.585206005937101E-4	0.0	0.0	3.9511659089931306	0.0
13	3.585206005937101E-4	0.0	0.0	4.073242173495289	0.0
14	3.585206005937101E-4	0.0	0.0	4.2068807273665945	0.0
15	3.585206005937101E-4	0.0	0.0	4.331377005922761	0.0
16	3.585206005937101E-4	0.0	0.0	4.576694726879007	0.0
17	3.585206005937101E-4	0.0	0.0	4.872026071618075	0.0
18	3.585206005937101E-4	0.0	0.0	5.300906340078301	0.0
19	3.585206005937101E-4	0.0	0.0	5.468425090705712	0.0
20	4.4815075074213764E-4	0.0	0.0	5.696623452983609	0.0
21	4.4815075074213764E-4	0.0	0.0	5.8994564827695	0.0
22	4.4815075074213764E-4	0.0	0.0	6.151586095137026	0.0
23	4.4815075074213764E-4	0.0	0.0	6.419580244080825	0.0
24	4.4815075074213764E-4	0.0	0.0	6.601170928281539	0.0
25	4.4815075074213764E-4	0.0	0.0	6.7642081714015285	0.0
26	4.4815075074213764E-4	0.0	0.0	6.907616411639013	0.0
27	4.4815075074213764E-4	0.0	0.0	7.0702055040082605	0.0
28	4.4815075074213764E-4	0.0	0.0	7.237544994335375	0.0
29	4.4815075074213764E-4	0.0	0.0	7.412144526824512	0.0
30	4.4815075074213764E-4	0.0	0.0	7.622596119373019	0.0
31	4.4815075074213764E-4	0.0	0.0	7.795044528258594	0.0
32	4.4815075074213764E-4	0.0	0.0	7.9791448566634635	0.0
33	4.4815075074213764E-4	0.0	0.0	8.170057076479614	0.0
34	4.4815075074213764E-4	0.0	0.0	8.354157404884484	0.0
35	4.4815075074213764E-4	0.0	0.0	8.569717915991452	0.0
36	4.4815075074213764E-4	0.0	0.0	8.751577490642612	0.0
37	4.4815075074213764E-4	0.0	0.0	8.949570492320488	0.0
38	5.377809008905651E-4	0.0	0.0	9.167102866730723	0.0
39	5.377809008905651E-4	0.0	0.0	9.377912979879824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	20	7.033197E-4	45.000004	23
TCCGCAA	20	7.033197E-4	45.000004	19
GATCGAC	20	7.033197E-4	45.000004	36
ACGTTGT	20	7.033197E-4	45.000004	40
CCGATAT	40	6.8139343E-9	45.000004	21
CCTAGCG	40	6.8139343E-9	45.000004	1
TAAACCG	40	6.8139343E-9	45.000004	1
ATCACGC	20	7.033197E-4	45.000004	42
AACGGCC	20	7.033197E-4	45.000004	33
TAACACG	55	1.8189894E-12	45.000004	1
CGAATTG	20	7.033197E-4	45.000004	2
CGTCGGA	20	7.033197E-4	45.000004	14
TTCGGAT	40	6.8139343E-9	45.000004	17
TAGGCGT	20	7.033197E-4	45.000004	33
ATACGTT	20	7.033197E-4	45.000004	17
CACGTAT	20	7.033197E-4	45.000004	45
ATCGGCG	20	7.033197E-4	45.000004	38
CCTATCG	20	7.033197E-4	45.000004	12
GATTCGC	40	6.8139343E-9	45.000004	23
TATACGA	40	6.8139343E-9	45.000004	23
>>END_MODULE