FastQCFastQC Report
Sat 18 Jun 2016
SRR3548582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728579
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136041.867196282077853No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28580.39227043326804645No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25580.35109439058770564No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15490.2126056337061595No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT10310.14150833334477111No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7450.10225383932284625No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA207.0315856E-445.035
GAAATCG207.0315856E-445.09
AATCCGA207.0315856E-445.024
TACCCGA253.889394E-545.015
GTCGATA253.889394E-545.011
GTACATC207.0315856E-445.014
GGTCGAT253.889394E-545.010
ACGTTTA253.889394E-545.039
CGACGAA253.889394E-545.021
TATGGTC253.889394E-545.035
CTATACG207.0315856E-445.01
ATTACGT351.2111013E-745.020
CCAATTA253.889394E-545.043
TGTATCG207.0315856E-445.027
CACGATT253.889394E-545.036
TAACGTG207.0315856E-445.016
TCGATTG253.889394E-545.012
TAACGCA253.889394E-545.035
TCGATAC253.889394E-545.012
ATCGTTC207.0315856E-445.022