##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548582_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728579
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.794947425056172	31.0	31.0	34.0	30.0	34.0
2	31.99874824830252	33.0	31.0	34.0	30.0	34.0
3	32.15521721048781	34.0	31.0	34.0	30.0	34.0
4	35.87273857742262	37.0	35.0	37.0	35.0	37.0
5	35.61129266695856	37.0	35.0	37.0	33.0	37.0
6	34.290481883227486	37.0	35.0	37.0	32.0	37.0
7	35.05117358584313	37.0	35.0	37.0	32.0	37.0
8	35.721518188144316	37.0	35.0	37.0	33.0	37.0
9	37.43134512523693	39.0	37.0	39.0	35.0	39.0
10	36.870560364764835	39.0	37.0	39.0	32.0	39.0
11	36.88149809423549	39.0	37.0	39.0	33.0	39.0
12	36.79154353886126	39.0	37.0	39.0	33.0	39.0
13	36.78235853627403	39.0	37.0	39.0	33.0	39.0
14	37.951782854021324	40.0	37.0	41.0	33.0	41.0
15	37.973043417391935	40.0	37.0	41.0	33.0	41.0
16	37.87210721143486	40.0	37.0	41.0	33.0	41.0
17	37.85367955980065	40.0	37.0	41.0	33.0	41.0
18	37.72565638043369	39.0	37.0	41.0	33.0	41.0
19	37.68291702066625	39.0	36.0	41.0	33.0	41.0
20	37.61570124859487	39.0	36.0	41.0	33.0	41.0
21	37.57983142528127	39.0	36.0	41.0	33.0	41.0
22	37.530892326020926	39.0	36.0	41.0	32.0	41.0
23	37.41578058110377	39.0	36.0	41.0	32.0	41.0
24	37.22672352620649	39.0	35.0	41.0	32.0	41.0
25	37.25305972310484	39.0	35.0	41.0	32.0	41.0
26	37.202553189153136	39.0	35.0	41.0	32.0	41.0
27	37.14700396250784	39.0	35.0	41.0	32.0	41.0
28	37.07144729672417	39.0	35.0	41.0	31.0	41.0
29	36.87270289151897	39.0	35.0	40.0	31.0	41.0
30	36.903753745304215	39.0	35.0	40.0	31.0	41.0
31	36.8328911483861	39.0	35.0	40.0	31.0	41.0
32	36.730664759758376	39.0	35.0	40.0	31.0	41.0
33	36.689820870488994	39.0	35.0	40.0	31.0	41.0
34	36.58257100465426	39.0	35.0	40.0	30.0	41.0
35	36.48422888938605	39.0	35.0	40.0	30.0	41.0
36	36.43277393391794	39.0	35.0	40.0	30.0	41.0
37	36.339628235235985	39.0	35.0	40.0	30.0	41.0
38	36.23582068656933	38.0	35.0	40.0	30.0	41.0
39	36.20859783221861	38.0	35.0	40.0	30.0	41.0
40	36.05404904615697	38.0	35.0	40.0	30.0	41.0
41	35.951671678706084	38.0	35.0	40.0	29.0	41.0
42	35.83808756497236	38.0	35.0	40.0	29.0	41.0
43	35.75013828287667	38.0	35.0	40.0	29.0	41.0
44	35.58180787532992	38.0	34.0	40.0	28.0	41.0
45	35.43691075367256	38.0	34.0	40.0	28.0	41.0
46	35.27130894522077	38.0	34.0	40.0	27.0	41.0
47	35.17573660509018	37.0	34.0	40.0	27.0	41.0
48	35.025338364130725	37.0	34.0	40.0	26.0	41.0
49	34.82216478926787	37.0	34.0	40.0	26.0	41.0
50	34.72613402252878	37.0	34.0	40.0	26.0	41.0
51	33.527325108190055	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	5.0
11	11.0
12	10.0
13	15.0
14	44.0
15	69.0
16	122.0
17	212.0
18	440.0
19	756.0
20	1230.0
21	1796.0
22	2509.0
23	3364.0
24	4163.0
25	4968.0
26	5898.0
27	7024.0
28	8776.0
29	11132.0
30	14176.0
31	18687.0
32	24288.0
33	33128.0
34	49951.0
35	65069.0
36	71626.0
37	99875.0
38	144587.0
39	154619.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.880537319906285	33.03595080286421	19.503993389872615	25.579518487356896
2	25.22746332243998	30.59970161094404	19.201349476172112	24.971485590443866
3	20.74078445851445	33.40214307576804	19.324328590310728	26.532743875406783
4	20.21263308440128	34.07578313401841	18.907352531434476	26.804231250145833
5	18.84462769308476	35.63827670026175	16.776629576202442	28.740466030451056
6	17.997499241674547	42.72494815249959	16.8440210327226	22.43353157310326
7	85.19501660080788	8.425304599775728	3.7006282091578266	2.6790505902585715
8	86.3107501039695	7.548666651111273	3.1151048822433807	3.025478362675839
9	80.36959615909875	10.938278484556925	4.9831246851748405	3.7090006711694956
10	35.99650827158071	38.16799550906628	11.09708075582744	14.738415463525575
11	25.386265593710494	31.96729524183376	23.483108901025147	19.163330263430595
12	25.00593621281975	27.107698684699944	28.38690107730253	19.499464025177776
13	24.35219790853154	27.085051861225757	26.882191224287276	21.680559005955427
14	20.384886196280704	30.153078801337944	27.804809087278116	21.657225915103233
15	20.15951598934364	31.080775042926025	27.95551340348816	20.804195564242175
16	22.090809644527223	31.4160852838196	25.53806793772535	20.955037133927824
17	22.657666498759916	29.53351661247442	26.194551311525586	21.61426557724008
18	22.431747277920444	29.248852904077665	25.620420023086034	22.698979794915857
19	22.189769400435644	31.499809903936292	25.496068374191406	20.81435232143666
20	23.30962050786531	30.140039721155837	25.963279205137674	20.587060565841178
21	24.48972589108388	28.831739591725814	26.89550481142059	19.783029705769724
22	21.822753606678205	28.818563258068103	25.444598320840978	23.91408481441271
23	21.10793750574749	30.33809648644828	26.186316102989515	22.367649904814716
24	20.920861018503143	30.270979536879324	27.5010671457728	21.307092298844736
25	22.043731702396034	30.62921110819829	25.337677863347697	21.989379326057986
26	22.380688984996823	31.036030409880055	23.542539655960436	23.040740949162686
27	21.372287699755276	29.717299016304338	26.334412603163145	22.576000680777238
28	22.373689057741164	30.297743964621542	26.915132058431553	20.41343491920574
29	21.14444693025739	30.990874016407282	26.3408635165164	21.52381553681893
30	24.100337780803454	29.92633605964487	25.72418365064049	20.249142508911184
31	23.15726914994805	29.243362765053615	24.201768099272694	23.39759998572564
32	22.41541411432391	29.072482187930206	26.681114882531613	21.830988815214273
33	21.881772601186693	29.53928125844967	26.03327847769425	22.545667662669388
34	20.54396297450242	28.621741774056076	28.32623504108683	22.508060210354678
35	21.83661620771392	28.38648931687573	28.588251926009395	21.188642549400956
36	24.24829702750148	29.200951441092865	25.882574161484207	20.66817736992145
37	22.9458987975223	30.06015819835598	25.542460048944587	21.45148295517713
38	20.745039316258087	30.074707066769697	27.46126363784847	21.718989979123744
39	21.824949662287825	28.224392962190787	27.192246825670242	22.75841054985115
40	22.46606064682073	27.40842104974203	28.472821753028843	21.652696550408397
41	20.59941337864528	27.951670306171327	27.878788710627123	23.570127604556266
42	22.297513378782536	28.654545354724746	26.696212764847736	22.35172850164498
43	21.49979617858873	28.04706147171412	26.88026967562886	23.57287267406829
44	22.007359531361733	27.360245079806035	27.31755924889408	23.31483613993815
45	23.44852102517366	26.650232850521355	27.63214421497188	22.2691019093331
46	21.240249856226985	27.290245807249452	29.349734208644495	22.11977012787906
47	21.680284499004227	27.965258400255838	28.035806686714825	22.31865041402511
48	20.75752938253779	28.94497370909675	27.71559432813737	22.58190258022809
49	21.67850020382141	29.07605077829583	27.37932331291459	21.866125704968166
50	21.64569662315274	27.674692792408234	26.957680635867902	23.721929948571123
51	22.007359531361733	27.28969679334705	27.486381023883478	23.21656265140774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15769.0
1	11744.0
2	7719.0
3	4829.0
4	1939.0
5	1744.0
6	1549.0
7	1548.0
8	1547.0
9	1614.0
10	1681.0
11	1774.0
12	1867.0
13	2022.0
14	2177.0
15	2154.5
16	2132.0
17	2097.0
18	2062.0
19	2472.0
20	2882.0
21	2943.0
22	3004.0
23	3968.0
24	4932.0
25	5643.5
26	6719.5
27	7084.0
28	9462.0
29	11840.0
30	12512.0
31	13184.0
32	15068.5
33	16953.0
34	21864.0
35	26775.0
36	27989.5
37	29204.0
38	27296.0
39	25388.0
40	29312.5
41	33237.0
42	36278.5
43	39320.0
44	40660.5
45	42001.0
46	44090.0
47	46179.0
48	49630.0
49	53081.0
50	50801.0
51	48521.0
52	46254.0
53	43987.0
54	41257.5
55	38528.0
56	36340.0
57	34152.0
58	33361.5
59	32571.0
60	31463.5
61	30356.0
62	27624.5
63	24893.0
64	21861.0
65	18829.0
66	17458.0
67	16087.0
68	13911.0
69	11735.0
70	11039.5
71	10344.0
72	8573.5
73	6803.0
74	5766.0
75	4221.5
76	3714.0
77	2705.5
78	1697.0
79	1178.0
80	659.0
81	606.5
82	554.0
83	398.0
84	242.0
85	200.0
86	158.0
87	99.0
88	40.0
89	38.0
90	36.0
91	33.0
92	30.0
93	25.0
94	20.0
95	26.5
96	33.0
97	16.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	728579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.246066512569005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5969492198833	15.710329950781968
2	8.611886104931656	3.487136377582302
3	3.1594078287153438	1.9189674312150622
4	1.6397531673520986	1.3279420676162512
5	1.0817408426805464	1.0950498525136396
6	0.7654233333800916	0.9298087030731367
7	0.5389485955808069	0.763811237908927
8	0.4186217124078775	0.6780354426412336
9	0.36164414592595195	0.6589684289068232
>10	3.560991749310366	16.471931724318516
>50	1.199084641066157	17.570902500814164
>100	1.0552766601273262	35.60571132253704
>500	0.006847999092325284	0.7866719859043784
>1k	0.0027391996369301136	1.108605780629434
>5k	0.0	0.0
>10k+	6.847999092325284E-4	1.886127193557131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13604	1.867196282077853	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2858	0.39227043326804645	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2558	0.35109439058770564	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1549	0.2126056337061595	No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	1031	0.14150833334477111	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	745	0.10225383932284625	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17335113968423466	0.0
2	0.0	0.0	0.0	0.24129161010679692	0.0
3	0.0	0.0	0.0	0.32021235857745006	0.0
4	0.0	0.0	0.0	0.7082279341018612	0.0
5	0.0	0.0	0.0	0.7723253072075917	0.0
6	0.0	0.0	0.0	1.1676153169388632	0.0
7	0.0	0.0	0.0	1.449122195396793	0.0
8	0.0	0.0	0.0	1.7040018995881023	0.0
9	0.0	0.0	0.0	2.1064290900506326	0.0
10	0.0	0.0	0.0	2.6808348854413864	0.0
11	0.0	0.0	0.0	3.232868364309155	0.0
12	0.0	0.0	0.0	3.4715521583795304	0.0
13	0.0	0.0	0.0	3.5856097966040745	0.0
14	0.0	0.0	0.0	3.7116084872059174	0.0
15	0.0	0.0	0.0	3.820450493357618	0.0
16	0.0	0.0	0.0	4.048840276757908	0.0
17	0.0	0.0	0.0	4.297406321071565	0.0
18	0.0	0.0	0.0	4.697637455924478	0.0
19	0.0	0.0	0.0	4.841616351830069	0.0
20	0.0	0.0	0.0	5.017849814501928	0.0
21	0.0	0.0	0.0	5.186671589491325	0.0
22	0.0	0.0	0.0	5.37333631630887	0.0
23	0.0	0.0	0.0	5.561099070931224	0.0
24	0.0	0.0	0.0	5.705764234214821	0.0
25	0.0	0.0	0.0	5.8327236991458715	0.0
26	0.0	0.0	0.0	5.945408802614404	0.0
27	0.0	0.0	0.0	6.0819760108375345	0.0
28	0.0	0.0	0.0	6.219503993389873	0.0
29	0.0	0.0	0.0	6.3453654305161145	0.0
30	0.0	0.0	0.0	6.5508338834910145	0.0
31	0.0	0.0	0.0	6.689459893848162	0.0
32	0.0	0.0	0.0	6.836870126643782	0.0
33	0.0	0.0	0.0	6.9897704984634474	0.0
34	0.0	0.0	0.0	7.13265136656423	0.0
35	0.0	0.0	0.0	7.289943849603132	0.0
36	0.0	0.0	0.0	7.425138523070251	0.0
37	0.0	0.0	0.0	7.569529179402646	0.0
38	0.0	0.0	0.0	7.719547228234687	0.0
39	1.3725347560113592E-4	0.0	0.0	7.8827416107244375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	20	7.0315856E-4	45.0	35
GAAATCG	20	7.0315856E-4	45.0	9
AATCCGA	20	7.0315856E-4	45.0	24
TACCCGA	25	3.889394E-5	45.0	15
GTCGATA	25	3.889394E-5	45.0	11
GTACATC	20	7.0315856E-4	45.0	14
GGTCGAT	25	3.889394E-5	45.0	10
ACGTTTA	25	3.889394E-5	45.0	39
CGACGAA	25	3.889394E-5	45.0	21
TATGGTC	25	3.889394E-5	45.0	35
CTATACG	20	7.0315856E-4	45.0	1
ATTACGT	35	1.2111013E-7	45.0	20
CCAATTA	25	3.889394E-5	45.0	43
TGTATCG	20	7.0315856E-4	45.0	27
CACGATT	25	3.889394E-5	45.0	36
TAACGTG	20	7.0315856E-4	45.0	16
TCGATTG	25	3.889394E-5	45.0	12
TAACGCA	25	3.889394E-5	45.0	35
TCGATAC	25	3.889394E-5	45.0	12
ATCGTTC	20	7.0315856E-4	45.0	22
>>END_MODULE