##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302465
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65483940290612	31.0	31.0	34.0	30.0	34.0
2	31.826965103400394	31.0	31.0	34.0	30.0	34.0
3	31.99978840527003	33.0	31.0	34.0	30.0	34.0
4	35.720701568776555	37.0	35.0	37.0	33.0	37.0
5	35.47546658291042	37.0	35.0	37.0	33.0	37.0
6	34.152986295935065	37.0	35.0	37.0	32.0	37.0
7	34.847400525680655	37.0	35.0	37.0	32.0	37.0
8	35.502835038764815	37.0	35.0	37.0	32.0	37.0
9	37.09938009356455	39.0	37.0	39.0	34.0	39.0
10	36.61909642437968	38.0	35.0	39.0	32.0	39.0
11	36.66606384209743	39.0	35.0	39.0	32.0	39.0
12	36.628743821599194	39.0	35.0	39.0	32.0	39.0
13	36.59221066900302	39.0	35.0	39.0	32.0	39.0
14	37.690870018018614	39.0	37.0	41.0	33.0	41.0
15	37.75114145438315	39.0	37.0	41.0	33.0	41.0
16	37.6237283652654	39.0	36.0	41.0	32.0	41.0
17	37.55758186897658	39.0	36.0	41.0	32.0	41.0
18	37.28205908121601	39.0	36.0	40.0	32.0	41.0
19	37.217119336121534	39.0	35.0	40.0	32.0	41.0
20	37.134894946522735	39.0	35.0	40.0	32.0	41.0
21	37.07472269518787	39.0	35.0	40.0	31.0	41.0
22	36.96404542674359	39.0	35.0	40.0	31.0	41.0
23	36.849020547831984	39.0	35.0	40.0	31.0	41.0
24	36.684518869951894	39.0	35.0	40.0	31.0	41.0
25	36.61768469079067	38.0	35.0	40.0	31.0	41.0
26	36.643750516588696	39.0	35.0	40.0	31.0	41.0
27	36.573494453903756	39.0	35.0	40.0	31.0	41.0
28	36.26473476269982	38.0	35.0	40.0	30.0	41.0
29	35.91704825351694	38.0	35.0	40.0	29.0	41.0
30	35.91873770518903	38.0	35.0	40.0	29.0	41.0
31	35.7874068073992	38.0	35.0	40.0	28.0	41.0
32	35.75718843502554	38.0	35.0	40.0	28.0	41.0
33	35.67722215793563	38.0	35.0	40.0	28.0	41.0
34	35.628796720281684	38.0	35.0	40.0	28.0	41.0
35	35.43392128014812	38.0	34.0	40.0	27.0	41.0
36	35.34719389020217	38.0	34.0	40.0	27.0	41.0
37	35.342423090274906	38.0	34.0	40.0	27.0	41.0
38	35.18478501644818	38.0	34.0	40.0	26.0	41.0
39	35.03271783512142	38.0	34.0	40.0	26.0	41.0
40	34.83898302282909	37.0	33.0	40.0	25.0	41.0
41	34.7170813151935	37.0	33.0	40.0	24.0	41.0
42	34.61327756930554	37.0	33.0	40.0	24.0	41.0
43	34.44451093514952	37.0	33.0	40.0	23.0	41.0
44	34.39123865571223	37.0	33.0	40.0	23.0	41.0
45	34.22836691848644	37.0	33.0	40.0	23.0	41.0
46	33.979895194485316	36.0	33.0	40.0	23.0	41.0
47	33.94583505529565	36.0	33.0	40.0	23.0	41.0
48	33.814107417387135	36.0	33.0	40.0	23.0	41.0
49	33.47381349908254	36.0	32.0	39.0	21.0	41.0
50	33.299052782966626	35.0	32.0	39.0	20.0	41.0
51	32.0751690278214	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	9.0
11	5.0
12	9.0
13	7.0
14	16.0
15	31.0
16	64.0
17	113.0
18	219.0
19	461.0
20	911.0
21	1309.0
22	1845.0
23	2364.0
24	2709.0
25	3099.0
26	3584.0
27	4282.0
28	4954.0
29	6222.0
30	7619.0
31	9609.0
32	11950.0
33	15942.0
34	22963.0
35	31714.0
36	29801.0
37	38639.0
38	52069.0
39	49940.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.859603590498075	34.606648703155734	19.862463425520307	24.67128428082588
2	20.886714165275322	35.24606152778008	18.167060651645645	25.700163655298958
3	19.905774221810788	33.73844907675268	20.41128725637677	25.94448944505976
4	19.665415833236903	34.77625510389632	18.042087514257847	27.516241548608928
5	16.523895326732017	38.657365314995126	15.050005785793397	29.768733572479462
6	16.40421205759344	44.51886995189526	17.98918883176566	21.087729158745642
7	78.96021027226291	12.718165738184585	4.707321508273685	3.6143024812788256
8	79.89420263501562	11.226753508670424	4.0755128692576	4.803530987056353
9	74.12626254277355	14.18544294381168	6.970062651877076	4.718231861537698
10	33.415105880019176	37.488635048683314	12.766105169193128	16.330153902104374
11	22.976873357247946	36.24849156100706	22.20323012579968	18.57140495594532
12	24.866017555750254	27.114211561668295	29.877506488354022	18.142264394227432
13	26.69631196998	28.628105731241632	24.887507645512706	19.788074653265667
14	17.865538161440167	34.270080835799185	26.961466615972096	20.902914386788556
15	17.312416312631214	32.604764187591954	29.459276279900156	20.62354321987668
16	18.494701866331642	33.78175987304316	25.497164961235185	22.226373299390012
17	19.800638090357563	29.090638586282708	25.55469227844544	25.55403104491429
18	23.11242623113418	30.442530540723723	26.061527780073728	20.38351544806837
19	22.197609640784886	33.239548377498224	23.465524936769544	21.09731704494735
20	22.23529995206057	30.29474484651117	24.224951647298035	23.245003554130232
21	23.93169457623196	31.702841651100126	24.262311341808143	20.10315243085977
22	20.055874233382376	33.974840064139656	22.16421734746169	23.80506835501628
23	20.16398591572579	31.494883705552706	23.41494057163639	24.926189807085116
24	17.564346288000266	33.49544575405419	24.93181029209991	24.008397665845635
25	18.99723935000744	33.640586514142136	24.134693270295738	23.227480865554693
26	20.479394310085464	30.854148413866067	23.566693667035857	25.099763609012616
27	17.938273849866924	31.963698279139734	24.109897012877525	25.988130858115817
28	21.746317755773394	30.417073049774352	26.777643694311738	21.058965500140513
29	21.494387780404345	32.825285570231266	23.635131337510128	22.045195311854265
30	21.26791529598466	32.013952027507315	24.161803845072985	22.556328831435042
31	21.73110938455689	35.642471029705916	21.414378523134907	21.212041062602285
32	23.451969649380917	31.672755525432695	22.901162117931	21.974112707255387
33	20.366653993023988	31.064751293538095	26.13856148645298	22.430033226984943
34	21.633577438711917	31.013175078108212	24.780057196700444	22.573190286479427
35	21.821367761559188	31.68069032780652	24.62202238275503	21.875919527879258
36	22.42474335873572	33.070603210288795	25.017109417618567	19.487544013356917
37	23.67216041525466	32.14983551815912	22.607905046864925	21.57009901972129
38	22.633362537814293	30.65181095333344	25.690906385862828	21.023920122989438
39	26.261881540012894	28.38906980973005	23.88177144462996	21.4672772056271
40	24.15519150976146	30.120179194286944	24.580034053526852	21.144595242424742
41	21.14856264361166	29.289008645628417	26.03111103764072	23.531317673119204
42	22.981501991966013	29.850726530342353	24.90436910055709	22.263402377134543
43	20.41393219050138	31.476369166680442	25.18671581835915	22.92298282445903
44	21.70069264212388	27.722215793562892	24.297356718959218	26.279734845354007
45	22.606251963037046	29.759476303043325	25.359297769989915	22.27497396392971
46	20.496586381895426	31.311722017423506	27.456069297274066	20.735622303407006
47	21.61770783396426	28.867472269518785	26.90493114905857	22.609888747458385
48	20.927380027441192	28.661828641330402	27.677913146975687	22.732878184252723
49	23.413287487808507	29.032780652306876	26.460912832889754	21.09301902699486
50	20.86158729109153	27.81677218851768	27.481857404989007	23.839783115401783
51	22.077265138115155	27.44515894401005	27.129419932884797	23.348155984989997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12045.0
1	9055.5
2	6066.0
3	3737.0
4	1408.0
5	1084.0
6	760.0
7	772.5
8	785.0
9	849.5
10	914.0
11	973.0
12	1032.0
13	1082.5
14	1133.0
15	1128.5
16	1124.0
17	1206.0
18	1288.0
19	1167.0
20	1046.0
21	1148.5
22	1251.0
23	1426.5
24	1602.0
25	1782.5
26	2151.5
27	2340.0
28	2883.0
29	3426.0
30	4025.0
31	4624.0
32	4731.5
33	4839.0
34	5586.0
35	6333.0
36	8421.5
37	10510.0
38	10521.0
39	10532.0
40	10679.5
41	10827.0
42	11576.5
43	12326.0
44	14979.0
45	17632.0
46	19468.0
47	21304.0
48	21935.5
49	22567.0
50	23073.5
51	23580.0
52	22137.0
53	20694.0
54	18738.0
55	16782.0
56	15528.5
57	14275.0
58	13810.0
59	13345.0
60	13280.0
61	13215.0
62	11769.5
63	10324.0
64	9353.5
65	8383.0
66	7136.5
67	5890.0
68	5431.0
69	4972.0
70	4600.0
71	4228.0
72	3568.0
73	2908.0
74	2253.5
75	1420.0
76	1241.0
77	944.0
78	647.0
79	478.0
80	309.0
81	213.5
82	118.0
83	111.0
84	104.0
85	90.0
86	76.0
87	69.0
88	62.0
89	38.0
90	14.0
91	10.0
92	6.0
93	8.0
94	10.0
95	7.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.79242226373299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31179165661925	23.411634403980624
2	7.86454924385959	4.528788454862545
3	2.6525198938992043	2.291174185442944
4	1.3446323791151378	1.5486089299588381
5	0.8784319129146715	1.2646091283288976
6	0.5913626603281776	1.0216058056304034
7	0.431752155890087	0.870183326996512
8	0.33529688702102495	0.772320764385962
9	0.2583623273278446	0.6694989502917693
>10	2.993558165971959	20.037029077744535
>50	0.9071388381733209	18.77539550030582
>100	0.4214176627969732	18.09035756203197
>500	0.0011482770103459759	0.3243350470302349
>1k	0.006889662062075854	2.9467872315805135
>5k	0.0	0.0
>10k+	0.0011482770103459759	3.4476716314284297
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10428	3.4476716314284297	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2533	0.83745226720447	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1905	0.6298249384226273	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1245	0.41161787314234705	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	1124	0.37161324450762895	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	1082	0.35772734035342935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGT	1024	0.33855156795001073	No Hit
AATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	981	0.3243350470302349	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTC	414	0.13687534094853948	No Hit
AGAGAAGGGACTGGAGCTAAGTCACTCCGACTTGGGTTGGCTTGCTTTTGA	340	0.11240970029590201	No Hit
TAACGTGGGAGGGTTTGTGTTGGGGGTTTTGTTGTTTTGGATTTTTGTTTT	328	0.10844229910898781	No Hit
GCTACAGGGGTGTTCTTTTTTAACTTTTGATTTTTTTTCCTTAATCTACTT	324	0.10711983204668307	No Hit
AGACCAGGGAGGATTAGGAAGCCATTTTCGGTCTTTTTACCTTTTTTTTTT	323	0.10678921528110691	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	320	0.10579736498437836	No Hit
TCATGAGGGACTTTTCAAATATATTCTCCTCAGACCCCCTATCCACACCAT	316	0.10447489792207362	No Hit
AGTGAAGGGAAAGTGAGAGGAAGAGCAGGTCTGGGAGAAAGAGCCAAGATG	315	0.10414428115649743	No Hit
AGGGGAGGGGAGGGGTGGTGATTTTTGTTTTCAAAAAGACTCAATTTTGTA	313	0.10348304762534508	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	308	0.10182996379746417	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.612335311523647E-4	0.0	0.0	0.4612103879787744	0.0
2	6.612335311523647E-4	0.0	0.0	0.6076736151290232	0.0
3	6.612335311523647E-4	0.0	0.0	0.8252194468781512	0.0
4	6.612335311523647E-4	0.0	0.0	1.8117798753574794	0.0
5	6.612335311523647E-4	0.0	0.0	2.052138263931364	0.0
6	6.612335311523647E-4	0.0	0.0	2.7870993338072174	0.0
7	6.612335311523647E-4	0.0	0.0	3.34286611674078	0.0
8	6.612335311523647E-4	0.0	0.0	3.8655712231167243	0.0
9	6.612335311523647E-4	0.0	0.0	4.836592663613972	0.0
10	6.612335311523647E-4	0.0	0.0	6.021192534673434	0.0
11	6.612335311523647E-4	0.0	0.0	7.197857603359067	0.0
12	6.612335311523647E-4	0.0	0.0	7.725521961218654	0.0
13	6.612335311523647E-4	0.0	0.0	8.120278379316614	0.0
14	6.612335311523647E-4	0.0	0.0	8.448580827533764	0.0
15	6.612335311523647E-4	0.0	0.0	8.684971814920734	0.0
16	6.612335311523647E-4	0.0	0.0	9.140231101119138	0.0
17	6.612335311523647E-4	0.0	0.0	9.643099201560512	0.0
18	6.612335311523647E-4	0.0	0.0	10.685203246656638	0.0
19	6.612335311523647E-4	0.0	0.0	10.912336964607475	0.0
20	6.612335311523647E-4	0.0	0.0	11.343791843684393	0.0
21	6.612335311523647E-4	0.0	0.0	11.610268956738796	0.0
22	6.612335311523647E-4	0.0	0.0	11.882366554807994	0.0
23	6.612335311523647E-4	0.0	0.0	12.182235961185592	0.0
24	6.612335311523647E-4	0.0	0.0	12.380606020531301	0.0
25	6.612335311523647E-4	0.0	0.0	12.547567487147273	0.0
26	6.612335311523647E-4	0.0	0.0	12.710230935810754	0.0
27	6.612335311523647E-4	0.0	0.0	12.959515977055197	0.0
28	6.612335311523647E-4	0.0	0.0	13.174086257914139	0.0
29	6.612335311523647E-4	0.0	0.0	13.35328054485643	0.0
30	6.612335311523647E-4	0.0	0.0	13.655133651827484	0.0
31	6.612335311523647E-4	0.0	0.0	13.836972872894384	0.0
32	6.612335311523647E-4	0.0	0.0	14.015505926305522	0.0
33	6.612335311523647E-4	0.0	0.0	14.221149554493909	0.0
34	6.612335311523647E-4	0.0	0.0	14.465475344254708	0.0
35	6.612335311523647E-4	0.0	0.0	14.683682409534988	0.0
36	6.612335311523647E-4	0.0	0.0	14.859570528821516	0.0
37	6.612335311523647E-4	0.0	0.0	15.032152480452284	0.0
38	6.612335311523647E-4	0.0	0.0	15.237134875109517	0.0
39	6.612335311523647E-4	0.0	0.0	15.461293042170169	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	20	7.025035E-4	45.000004	24
TCCGGAT	20	7.025035E-4	45.000004	9
CTTAAGT	35	1.2079545E-7	45.000004	22
GTTGACG	20	7.025035E-4	45.000004	16
GTGCACC	20	7.025035E-4	45.000004	25
TCCATTA	40	6.7866495E-9	45.000004	23
GGTACCA	20	7.025035E-4	45.000004	38
GGTAATT	40	6.7866495E-9	45.000004	8
ATTAGAC	40	6.7866495E-9	45.000004	24
GGTAACC	35	1.2079545E-7	45.000004	6
AGTCAAA	20	7.025035E-4	45.000004	14
ATAGGCG	20	7.025035E-4	45.000004	26
AGATACG	20	7.025035E-4	45.000004	1
ACCATAC	20	7.025035E-4	45.000004	41
GAGTCGA	35	1.2079545E-7	45.000004	29
TCACACG	20	7.025035E-4	45.000004	1
CGCATGT	20	7.025035E-4	45.000004	12
GAGTAGC	40	6.7866495E-9	45.000004	9
GCACGAC	20	7.025035E-4	45.000004	36
GTGATCG	20	7.025035E-4	45.000004	23
>>END_MODULE