FastQCFastQC Report
Sat 18 Jun 2016
SRR3548579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298611
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101213.38935940069187No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24270.8127630931211509No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18260.6114979019527077No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12580.42128387768702424No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT10780.36100478549015275No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGT10170.3405768709123241No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC9810.32852105247294977No Hit
AATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT8630.2890047586994451No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTC4280.14333028589033894No Hit
AGAGAAGGGACTGGAGCTAAGTCACTCCGACTTGGGTTGGCTTGCTTTTGA3490.11687446209282311No Hit
AGTGAAGGGAAAGTGAGAGGAAGAGCAGGTCTGGGAGAAAGAGCCAAGATG3150.10548841134452515No Hit
AGACCAGGGAGGATTAGGAAGCCATTTTCGGTCTTTTTACCTTTTTTTTTT3050.1021395728891434No Hit
TCATGAGGGACTTTTCAAATATATTCTCCTCAGACCCCCTATCCACACCAT3040.10180468904360522No Hit
GCTACAGGGGTGTTCTTTTTTAACTTTTGATTTTTTTTCCTTAATCTACTT3010.1008000375069907No Hit
AGGGGAGGGGAGGGGTGGTGATTTTTGTTTTCAAAAAGACTCAATTTTGTA3000.10046515366145252No Hit
TAACGTGGGAGGGTTTGTGTTGGGGGTTTTGTTGTTTTGGATTTTTGTTTT2990.10013026981591434No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA207.024891E-445.010
GTCATCG207.024891E-445.037
CGGGTGT453.8198777E-1045.06
ACTATTA207.024891E-445.020
AACCGCT207.024891E-445.018
TCGTTGA253.8838487E-545.024
CTGTCGA406.7866495E-945.012
TTGGCGA253.8838487E-545.025
GTCGCAT253.8838487E-545.043
CAAACGT351.2078999E-745.017
GCCCACG207.024891E-445.01
TGCCCGA207.024891E-445.016
GATAATC351.2078999E-745.09
ATGATGG253.8838487E-545.032
AGTTTCG207.024891E-445.010
ATAGGTT207.024891E-445.05
CGAGTTC207.024891E-445.040
GTACAGA207.024891E-445.029
ACGTTTT253.8838487E-545.013
CATGCGG453.8198777E-1045.02