##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548579_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298611
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63663763223726	31.0	31.0	34.0	30.0	34.0
2	31.821255747444	31.0	31.0	34.0	30.0	34.0
3	31.99649711497567	33.0	31.0	34.0	30.0	34.0
4	35.723071152770665	37.0	35.0	37.0	35.0	37.0
5	35.49478418410574	37.0	35.0	37.0	33.0	37.0
6	32.82134951492075	37.0	35.0	37.0	27.0	37.0
7	34.21265124191674	37.0	35.0	37.0	28.0	37.0
8	35.23686669278761	37.0	35.0	37.0	32.0	37.0
9	37.00515051354438	39.0	37.0	39.0	33.0	39.0
10	36.56940970024547	38.0	35.0	39.0	32.0	39.0
11	36.674904809266906	39.0	35.0	39.0	32.0	39.0
12	36.64351614642461	39.0	35.0	39.0	32.0	39.0
13	36.63014758331073	39.0	35.0	39.0	32.0	39.0
14	37.701407516802796	39.0	37.0	41.0	33.0	41.0
15	37.81644011774516	40.0	37.0	41.0	33.0	41.0
16	37.69748937581	39.0	36.0	41.0	33.0	41.0
17	37.657865919205925	39.0	36.0	41.0	33.0	41.0
18	37.39881986932832	39.0	36.0	40.0	32.0	41.0
19	37.32726858689064	39.0	35.0	40.0	32.0	41.0
20	37.24130055490253	39.0	35.0	40.0	32.0	41.0
21	37.16132024607265	39.0	35.0	40.0	32.0	41.0
22	37.01325805144486	39.0	35.0	40.0	31.0	41.0
23	36.91296703738308	39.0	35.0	40.0	31.0	41.0
24	36.82287323641795	39.0	35.0	40.0	31.0	41.0
25	36.706534588477986	39.0	35.0	40.0	31.0	41.0
26	36.739942600908876	39.0	35.0	40.0	31.0	41.0
27	36.644078751285114	39.0	35.0	40.0	31.0	41.0
28	36.37126227767899	38.0	35.0	40.0	30.0	41.0
29	35.98962194962677	38.0	35.0	40.0	29.0	41.0
30	36.02621470742873	38.0	35.0	40.0	30.0	41.0
31	35.56221304640486	38.0	34.0	40.0	27.0	41.0
32	35.73870687951884	38.0	35.0	40.0	28.0	41.0
33	35.68187709093101	38.0	35.0	40.0	28.0	41.0
34	35.65134238189484	38.0	35.0	40.0	28.0	41.0
35	35.56456727983899	38.0	34.0	40.0	28.0	41.0
36	35.45372407580431	38.0	34.0	40.0	27.0	41.0
37	35.411404804243645	38.0	34.0	40.0	27.0	41.0
38	35.2597894920147	38.0	34.0	40.0	26.0	41.0
39	35.104540690061654	38.0	34.0	40.0	26.0	41.0
40	34.93922193087327	38.0	34.0	40.0	25.0	41.0
41	34.87352106921714	37.0	33.0	40.0	25.0	41.0
42	34.75346855943016	37.0	33.0	40.0	24.0	41.0
43	34.62170181272626	37.0	33.0	40.0	24.0	41.0
44	34.51228521387357	37.0	33.0	40.0	24.0	41.0
45	34.347485524645776	37.0	33.0	40.0	23.0	41.0
46	33.99940390675494	36.0	33.0	40.0	23.0	41.0
47	34.028451731516924	36.0	33.0	40.0	23.0	41.0
48	34.02859908040896	36.0	33.0	40.0	23.0	41.0
49	33.742430787881226	36.0	33.0	40.0	22.0	41.0
50	33.53697285096664	36.0	33.0	40.0	21.0	41.0
51	32.359899668799876	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	6.0
12	8.0
13	10.0
14	18.0
15	29.0
16	61.0
17	105.0
18	192.0
19	443.0
20	770.0
21	1259.0
22	1701.0
23	2243.0
24	2630.0
25	3001.0
26	3470.0
27	4011.0
28	4717.0
29	5965.0
30	7546.0
31	9487.0
32	12081.0
33	15909.0
34	22749.0
35	31437.0
36	30026.0
37	39539.0
38	50738.0
39	48443.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.984826412958665	34.108254551908665	20.06624002464745	24.840679010485214
2	20.96037989223438	35.048943274025405	18.17615560042999	25.814521233310227
3	20.124509813771095	33.68194741653857	20.361272692566583	25.83227007712375
4	19.69351430456346	34.68626406930756	18.04588578451564	27.574335841613333
5	16.694294583923565	38.7025260288469	14.900321823375561	29.70285756385398
6	15.787764014051724	46.7029010987539	17.276322707468914	20.233012179725463
7	79.3560853417992	12.512934888533911	4.583890077726541	3.5470896919403505
8	79.99805767369588	11.131204141843401	3.9938247418882757	4.876913442572444
9	74.40114396321636	13.97202380354374	6.9639095679663505	4.66292266527355
10	33.51885898376148	37.47919534109594	12.69511170050668	16.306833974635897
11	23.04871555301044	36.05995760370516	22.138836144683218	18.75249069860119
12	24.949516260285122	26.986614692693838	29.751080837611475	18.312788209409568
13	26.638670377179675	28.537126897535593	24.97898603869248	19.845216686592256
14	18.099802083647287	33.98803125136047	26.87978674596716	21.032379919025086
15	17.261252934419698	32.48172371412976	29.635880794746345	20.621142556704207
16	18.619541811922534	33.61430087973986	25.525181590765243	22.240975717572358
17	19.72733757296282	29.12685734952832	25.676883972794034	25.46892110471483
18	23.135115585159287	30.35420664342573	26.109888785074897	20.40078898634009
19	22.258724561385883	33.17794722900362	23.601273898148428	20.96205431146207
20	22.377608326551936	30.003248373301723	24.340027661405642	23.279115638740702
21	24.016194982770227	31.58390012424191	24.251618326183564	20.148286566804305
22	20.138574935283696	33.878524233869484	22.221552454531146	23.761348376315674
23	20.209905194383328	31.611025715730502	23.467320359933158	24.711748729953015
24	17.490983252458886	33.43111941623048	24.919711598032222	24.158185733278412
25	18.985904738941297	33.63104507201677	24.23956250774419	23.143487681297742
26	20.498575069237233	30.833760310236396	23.50181339602359	25.165851224502784
27	18.04253694606026	32.01188167883969	23.992418229737016	25.95316314536303
28	21.669328993238697	30.45333226170503	26.87442860443855	21.002910140617725
29	21.554128950373563	32.58118421625459	23.61701343888872	22.247673394483122
30	21.27349628781257	31.945239793577596	24.483357947295982	22.29790597131385
31	21.830408122942558	35.398227124921725	21.41180331601984	21.359561436115886
32	23.3966598685246	31.626430372625254	23.142483029761127	21.834426729089014
33	20.201198214399334	31.103676689740162	26.23044696946864	22.46467812639186
34	21.74735692924909	31.130467397383217	24.774372009068653	22.347803664299036
35	21.773142985355527	31.72254203629471	24.58248356557528	21.921831412774477
36	22.60231538690805	32.88190990954787	25.148437264534795	19.36733743900928
37	23.922762389865078	32.087230544085784	22.551747926231787	21.438259139817355
38	22.44056648951311	30.830411471781012	25.643730472085757	21.085291566620118
39	26.253219070965233	28.314429140252706	23.868846090733427	21.563505698048633
40	24.185981092458082	30.15528563917605	24.498762604190734	21.15997066417513
41	21.043765969773386	29.2986527622894	26.01813061139744	23.63945065653978
42	22.922464343242545	29.850876223581853	24.88521856194179	22.34144087123381
43	20.274537776572195	31.605332690356352	25.227804735927347	22.892324797144113
44	21.659282477872548	27.80875453349006	24.317925327600122	26.21403766103727
45	22.634799119925255	29.833127379768325	25.342000127255858	22.190073373050556
46	20.74136585725241	31.34244887160888	27.275284567547747	20.640900703590958
47	21.561831278820943	28.808382812421513	26.86907046290994	22.760715445847605
48	20.88101242084183	28.82378746931627	27.506689304814625	22.788510805027276
49	23.377236605483386	29.08566663652712	26.54423313273791	20.99286362525158
50	20.965068266071913	27.78497778045685	27.66374982837203	23.586204125099208
51	22.133143119309068	27.46717301104112	27.102149619404507	23.297534250245302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11743.0
1	8847.0
2	5951.0
3	3668.0
4	1385.0
5	1076.5
6	768.0
7	769.0
8	770.0
9	812.5
10	855.0
11	915.0
12	975.0
13	1071.0
14	1167.0
15	1181.0
16	1195.0
17	1202.5
18	1210.0
19	1107.0
20	1004.0
21	1086.5
22	1169.0
23	1350.5
24	1532.0
25	1757.5
26	2073.5
27	2164.0
28	2761.5
29	3359.0
30	4018.5
31	4678.0
32	4728.5
33	4779.0
34	5591.5
35	6404.0
36	8436.0
37	10468.0
38	10395.0
39	10322.0
40	10592.5
41	10863.0
42	11548.0
43	12233.0
44	14842.0
45	17451.0
46	19284.5
47	21118.0
48	21678.0
49	22238.0
50	22773.5
51	23309.0
52	21824.5
53	20340.0
54	18444.5
55	16549.0
56	15399.0
57	14249.0
58	13718.0
59	13187.0
60	13115.0
61	13043.0
62	11528.0
63	10013.0
64	9246.0
65	8479.0
66	7171.5
67	5864.0
68	5391.0
69	4918.0
70	4511.5
71	4105.0
72	3426.0
73	2747.0
74	2128.0
75	1361.0
76	1213.0
77	936.5
78	660.0
79	478.5
80	297.0
81	211.5
82	126.0
83	105.0
84	84.0
85	85.0
86	86.0
87	56.5
88	27.0
89	18.0
90	9.0
91	7.0
92	5.0
93	6.0
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	298611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.236699250864838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11770365618986	23.71613905716802
2	7.706405204801612	4.506197025561684
3	2.7009071749289837	2.3689683233370507
4	1.4123064235315679	1.6516471261942796
5	0.9277925410061394	1.356279574429609
6	0.6082195546595803	1.0669399318846258
7	0.4535874644918904	0.9282980198318214
8	0.37226243929258684	0.8706979983992552
9	0.31842756345642814	0.8378793815365141
>10	3.0892055346834053	20.597700687516536
>50	0.8980115458627326	18.86434190301094
>100	0.3860075139741593	16.68089923010204
>500	0.002290845780262073	0.6175258111723949
>1k	0.005727114450655182	2.5471265291633594
>5k	0.0	0.0
>10k+	0.0011454228901310365	3.38935940069187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10121	3.38935940069187	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2427	0.8127630931211509	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1826	0.6114979019527077	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1258	0.42128387768702424	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	1078	0.36100478549015275	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGT	1017	0.3405768709123241	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	981	0.32852105247294977	No Hit
AATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	863	0.2890047586994451	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTC	428	0.14333028589033894	No Hit
AGAGAAGGGACTGGAGCTAAGTCACTCCGACTTGGGTTGGCTTGCTTTTGA	349	0.11687446209282311	No Hit
AGTGAAGGGAAAGTGAGAGGAAGAGCAGGTCTGGGAGAAAGAGCCAAGATG	315	0.10548841134452515	No Hit
AGACCAGGGAGGATTAGGAAGCCATTTTCGGTCTTTTTACCTTTTTTTTTT	305	0.1021395728891434	No Hit
TCATGAGGGACTTTTCAAATATATTCTCCTCAGACCCCCTATCCACACCAT	304	0.10180468904360522	No Hit
GCTACAGGGGTGTTCTTTTTTAACTTTTGATTTTTTTTCCTTAATCTACTT	301	0.1008000375069907	No Hit
AGGGGAGGGGAGGGGTGGTGATTTTTGTTTTCAAAAAGACTCAATTTTGTA	300	0.10046515366145252	No Hit
TAACGTGGGAGGGTTTGTGTTGGGGGTTTTGTTGTTTTGGATTTTTGTTTT	299	0.10013026981591434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4286513222888641	0.0
2	0.0	0.0	0.0	0.564279279731825	0.0
3	0.0	0.0	0.0	0.757507258607352	0.0
4	0.0	0.0	0.0	1.6767634146096426	0.0
5	0.0	0.0	0.0	1.882382095770082	0.0
6	0.0	0.0	0.0	2.5635358375947304	0.0
7	0.0	0.0	0.0	3.110066273513032	0.0
8	0.0	0.0	0.0	3.6505688002116465	0.0
9	0.0	0.0	0.0	4.635462189939419	0.0
10	0.0	0.0	0.0	5.825639376982093	0.0
11	0.0	0.0	0.0	6.9384583957054495	0.0
12	0.0	0.0	0.0	7.50541674620158	0.0
13	0.0	0.0	0.0	7.908282012384005	0.0
14	0.0	0.0	0.0	8.237137948702493	0.0
15	0.0	0.0	0.0	8.466868266741681	0.0
16	0.0	0.0	0.0	8.906235872087766	0.0
17	0.0	0.0	0.0	9.376747675068902	0.0
18	0.0	0.0	0.0	10.439334116961533	0.0
19	0.0	0.0	0.0	10.648301636577353	0.0
20	0.0	0.0	0.0	11.062218069662537	0.0
21	0.0	0.0	0.0	11.318404211499242	0.0
22	0.0	0.0	0.0	11.584971752547629	0.0
23	0.0	0.0	0.0	11.861250925116623	0.0
24	0.0	0.0	0.0	12.049455646309077	0.0
25	0.0	0.0	0.0	12.222590594452313	0.0
26	0.0	0.0	0.0	12.380990653391871	0.0
27	0.0	0.0	0.0	12.646888426749182	0.0
28	0.0	0.0	0.0	12.827390819494259	0.0
29	0.0	0.0	0.0	13.003874606092877	0.0
30	0.0	0.0	0.0	13.296563087093242	0.0
31	0.0	0.0	0.0	13.482423621366928	0.0
32	0.0	0.0	0.0	13.68234927715322	0.0
33	0.0	0.0	0.0	13.898014473679805	0.0
34	0.0	0.0	0.0	14.12473083710915	0.0
35	0.0	0.0	0.0	14.35010766515634	0.0
36	0.0	0.0	0.0	14.536303083275566	0.0
37	0.0	0.0	0.0	14.718145011402795	0.0
38	0.0	0.0	0.0	14.899317171838948	0.0
39	0.0	0.0	0.0	15.124024232195064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCATA	20	7.024891E-4	45.0	10
GTCATCG	20	7.024891E-4	45.0	37
CGGGTGT	45	3.8198777E-10	45.0	6
ACTATTA	20	7.024891E-4	45.0	20
AACCGCT	20	7.024891E-4	45.0	18
TCGTTGA	25	3.8838487E-5	45.0	24
CTGTCGA	40	6.7866495E-9	45.0	12
TTGGCGA	25	3.8838487E-5	45.0	25
GTCGCAT	25	3.8838487E-5	45.0	43
CAAACGT	35	1.2078999E-7	45.0	17
GCCCACG	20	7.024891E-4	45.0	1
TGCCCGA	20	7.024891E-4	45.0	16
GATAATC	35	1.2078999E-7	45.0	9
ATGATGG	25	3.8838487E-5	45.0	32
AGTTTCG	20	7.024891E-4	45.0	10
ATAGGTT	20	7.024891E-4	45.0	5
CGAGTTC	20	7.024891E-4	45.0	40
GTACAGA	20	7.024891E-4	45.0	29
ACGTTTT	25	3.8838487E-5	45.0	13
CATGCGG	45	3.8198777E-10	45.0	2
>>END_MODULE