SRR3548577_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623120
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12320	1.9771472589549366	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGT	5725	0.918763641032225	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	4922	0.7898960071896264	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	4302	0.6903967133136475	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2966	0.4759917832841186	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2321	0.37248042110668894	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	2161	0.346803183977404	TruSeq Adapter, Index 13 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	1376	0.22082423931185005	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1150	0.18455514186673513	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	1105	0.17733341892412377	No Hit
AACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	926	0.1486070098857363	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT	837	0.1343240467325716	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	55	1.8189894E-12	45.000004	2
TTAGGTA	30	2.1638953E-6	45.000004	37
CGTGACG	30	2.1638953E-6	45.000004	1
ATTCGCA	30	2.1638953E-6	45.000004	15
GATATCA	30	2.1638953E-6	45.000004	9
CGCACTG	55	1.8189894E-12	45.000004	1
ACGTACT	30	2.1638953E-6	45.000004	27
AGGATAC	30	2.1638953E-6	45.000004	45
ATTGCCA	30	2.1638953E-6	45.000004	38
TATAAGC	30	2.1638953E-6	45.000004	21
TACGTAG	30	2.1638953E-6	45.000004	1
TCAAACG	30	2.1638953E-6	45.000004	1
ACAACGT	45	3.8380676E-10	45.0	36
CTAGATC	20	7.030798E-4	45.0	16
ATGATTG	20	7.030798E-4	45.0	28
ATTAGTG	20	7.030798E-4	45.0	12
ATTAGCC	20	7.030798E-4	45.0	42
CGTATAG	20	7.030798E-4	45.0	1
CTACTAG	85	0.0	45.0	1
CAAGTCG	20	7.030798E-4	45.0	30