##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623120
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614281358325844	31.0	31.0	34.0	30.0	34.0
2	31.813702015663115	31.0	31.0	34.0	30.0	34.0
3	31.97312235203492	33.0	31.0	34.0	30.0	34.0
4	35.6981287713442	37.0	35.0	37.0	33.0	37.0
5	35.44630729233535	37.0	35.0	37.0	33.0	37.0
6	32.670469572474005	37.0	35.0	37.0	26.0	37.0
7	34.11404223905508	37.0	35.0	37.0	26.0	37.0
8	35.21020509693157	37.0	35.0	37.0	32.0	37.0
9	37.091446270381304	39.0	37.0	39.0	34.0	39.0
10	36.6551386570805	38.0	35.0	39.0	32.0	39.0
11	36.68450860187444	39.0	35.0	39.0	32.0	39.0
12	36.51849563486969	39.0	35.0	39.0	32.0	39.0
13	36.56286108614713	39.0	35.0	39.0	32.0	39.0
14	37.663649056361535	39.0	36.0	41.0	32.0	41.0
15	37.72945339581461	39.0	37.0	41.0	33.0	41.0
16	37.573661574014636	39.0	36.0	41.0	32.0	41.0
17	37.568579085890356	39.0	36.0	41.0	32.0	41.0
18	37.41520894851714	39.0	36.0	41.0	32.0	41.0
19	37.32480581589421	39.0	35.0	41.0	32.0	41.0
20	37.291295416613174	39.0	35.0	41.0	32.0	41.0
21	37.25434587238413	39.0	35.0	40.0	32.0	41.0
22	37.14569424829888	39.0	35.0	40.0	31.0	41.0
23	36.99743869559635	39.0	35.0	40.0	31.0	41.0
24	36.91708178200026	39.0	35.0	40.0	31.0	41.0
25	36.92096546411606	39.0	35.0	40.0	31.0	41.0
26	36.858613108229555	39.0	35.0	40.0	31.0	41.0
27	36.75427044550006	39.0	35.0	40.0	31.0	41.0
28	36.667540762613946	39.0	35.0	40.0	31.0	41.0
29	36.43139042239055	39.0	35.0	40.0	30.0	41.0
30	36.48301129798433	39.0	35.0	40.0	30.0	41.0
31	36.070232058030555	38.0	35.0	40.0	29.0	41.0
32	36.140951983566566	38.0	35.0	40.0	30.0	41.0
33	36.15312780844781	38.0	35.0	40.0	30.0	41.0
34	36.07006034150725	38.0	35.0	40.0	30.0	41.0
35	36.033170175889076	38.0	35.0	40.0	30.0	41.0
36	35.9741735139299	38.0	35.0	40.0	29.0	41.0
37	35.80859224547439	38.0	35.0	40.0	29.0	41.0
38	35.6991574656567	38.0	35.0	40.0	28.0	41.0
39	35.5923112723071	38.0	35.0	40.0	28.0	41.0
40	35.50127262806522	38.0	34.0	40.0	27.0	41.0
41	35.47137950956477	38.0	34.0	40.0	27.0	41.0
42	35.35789414558993	38.0	34.0	40.0	27.0	41.0
43	35.313021568879186	38.0	34.0	40.0	27.0	41.0
44	35.121946013608934	38.0	34.0	40.0	26.0	41.0
45	34.91605629734241	37.0	34.0	40.0	26.0	41.0
46	34.6249374117345	37.0	33.0	40.0	24.0	41.0
47	34.60441808961356	37.0	33.0	40.0	24.0	41.0
48	34.605267043266146	37.0	33.0	40.0	25.0	41.0
49	34.47740884580819	37.0	33.0	40.0	24.0	41.0
50	34.307921427654385	36.0	33.0	40.0	24.0	41.0
51	33.09971273590961	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	4.0
11	7.0
12	9.0
13	10.0
14	28.0
15	46.0
16	96.0
17	212.0
18	430.0
19	847.0
20	1436.0
21	2287.0
22	3125.0
23	4007.0
24	4740.0
25	5614.0
26	6276.0
27	7059.0
28	8575.0
29	10873.0
30	13710.0
31	18075.0
32	23893.0
33	32144.0
34	46100.0
35	61334.0
36	64726.0
37	86178.0
38	112198.0
39	109065.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.589164205931443	35.490756194633455	17.769290024393374	27.150789575041724
2	22.739921684426754	34.61500192579278	18.6521055334446	23.99297085633586
3	18.928135832584413	32.35043009372192	22.98818847092053	25.73324560277314
4	22.655186801900115	32.57719219411992	18.70859545512903	26.059025548850943
5	17.690974451149057	37.76559892155604	17.23199383746309	27.311432789831812
6	17.142765438438822	42.73927975349852	19.455963538323275	20.661991269739378
7	78.42052895108486	12.712960585441008	5.261426370522532	3.6050840929515986
8	79.59317627423289	8.30353703941456	4.45163050455771	7.651656181794839
9	75.738381050199	10.929355501348056	7.987707022724355	5.344556425728592
10	36.016337142123504	32.741686994479394	14.034856849403004	17.20711901399409
11	26.450763897804595	31.08807292335345	22.316086789061497	20.14507638978046
12	24.382622929772758	27.675568108871484	29.345872384131468	18.59593657722429
13	24.746116317884194	29.226312748748235	24.60457054820901	21.42300038515856
14	20.02359096161253	29.372191552188987	28.412183849017847	22.19203363718064
15	19.35839003723199	32.069103864424186	25.259982025934008	23.31252407240981
16	20.73099884452433	32.0663756579792	26.168795737578638	21.033829759917833
17	19.767139555783796	29.118949801001413	28.643439465913467	22.47047117730132
18	22.096064963409937	27.836532289125692	26.445307484914622	23.62209526254975
19	20.764218770060342	32.474643728334826	25.968513287970215	20.792624213634614
20	21.485909616125305	32.62100397997176	25.070612402105535	20.822474001797406
21	21.163981255616896	30.70130953909359	26.041211965592503	22.093497239697008
22	22.22140197714726	30.06403261009115	25.57260238798305	22.141963024778534
23	21.66885993067146	30.325298497881626	25.038034407497754	22.967807163949157
24	18.161991269739374	32.660161766593916	27.048241109256644	22.129605854410066
25	20.843176274232892	31.19527538836821	25.70179098728977	22.259757350109126
26	23.669116703042754	29.67133136474515	24.502343047888047	22.15720888432405
27	19.69957632558737	29.761843625625882	27.547663371421233	22.990916677365515
28	21.125625882655026	30.108967775067402	27.144049300295286	21.621357041982282
29	20.274906920015408	32.56178585184234	24.919919116703042	22.24338811143921
30	21.47419437668507	28.013865708049813	27.79336243420208	22.718577481063036
31	24.777891898831687	31.16349980742072	22.508505584799078	21.55010270894852
32	22.620843497239697	28.835858261651047	25.43250096289639	23.110797278212864
33	22.42906663243035	28.523398382334058	25.517877776351266	23.529657208884323
34	20.036911028373346	29.294036461676725	27.185774810630374	23.48327769931955
35	22.340159198870204	28.474451149056364	27.628386185646427	21.557003466427012
36	22.39006932854025	30.19803569135961	26.314032610091154	21.097862370008986
37	22.087238413146746	30.789093593529337	25.080722814225187	22.04294517909873
38	23.029272050327386	29.36208114006933	25.70082809089742	21.90781871870587
39	20.358036975221466	28.274810630376173	27.558415714469124	23.80873667993324
40	22.854345872384133	28.063936320451923	26.032706380793424	23.049011426370523
41	20.92486198485043	25.519001155475674	30.48834895365259	23.067787906021312
42	23.408974194376686	26.793555013480553	26.732732058030557	23.064738734112208
43	22.39745153421492	26.75423674412633	26.943766850686867	23.904544870971883
44	21.751027089485174	26.04859417126717	27.541885993067144	24.65849274618051
45	24.477307741686992	27.775709333675696	27.590030812684557	20.15695211195275
46	21.382237771215816	27.89879958916421	29.271568879188596	21.44739376043138
47	20.861792271151625	28.83617922711516	26.71620233662858	23.585826165104635
48	20.09532674284247	27.865579663628193	29.90724098087046	22.13185261265888
49	21.977147258954936	27.369367056104764	27.544614199512136	23.108871485428168
50	21.095455129028117	27.342726922583132	27.06894338169213	24.492874566696624
51	20.19466555398639	26.487995891642058	29.291308255231733	24.026030299139816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14456.0
1	11179.5
2	7903.0
3	4930.0
4	1957.0
5	1704.5
6	1452.0
7	1468.5
8	1485.0
9	1520.0
10	1555.0
11	1666.5
12	1778.0
13	1922.5
14	2067.0
15	2337.5
16	2608.0
17	2371.0
18	2134.0
19	2303.0
20	2472.0
21	3400.5
22	4329.0
23	3645.0
24	2961.0
25	3883.0
26	5038.0
27	5271.0
28	7323.5
29	9376.0
30	10280.0
31	11184.0
32	12139.5
33	13095.0
34	14795.0
35	16495.0
36	17770.0
37	19045.0
38	20955.5
39	22866.0
40	25204.5
41	27543.0
42	29594.0
43	31645.0
44	38619.5
45	45594.0
46	46194.5
47	46795.0
48	47347.5
49	47900.0
50	46680.5
51	45461.0
52	43872.0
53	42283.0
54	37072.0
55	31861.0
56	28511.0
57	25161.0
58	25619.5
59	26078.0
60	25946.5
61	25815.0
62	22654.0
63	19493.0
64	17313.5
65	15134.0
66	14488.0
67	13842.0
68	11679.5
69	9517.0
70	8127.0
71	6737.0
72	5952.5
73	5168.0
74	4002.0
75	2266.0
76	1696.0
77	1545.5
78	1395.0
79	1131.0
80	867.0
81	617.0
82	367.0
83	373.0
84	379.0
85	257.5
86	136.0
87	88.5
88	41.0
89	44.5
90	48.0
91	33.5
92	19.0
93	13.0
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.60591616092809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20869988023824	17.679793125478525
2	8.706067009806286	3.9361724183020566
3	3.2034274479572513	2.172492369484124
4	1.6436389788305583	1.4862385981710824
5	1.0747210091331254	1.2147526514425733
6	0.780615094001029	1.0587911621365358
7	0.5957746054676698	0.9427621547408508
8	0.43606455644500214	0.7886111042998409
9	0.3857263958249747	0.7847728708568708
>10	3.150249715445984	15.75560700647113
>50	0.9602989573637786	15.76321591300593
>100	0.8439426139883619	31.623851890572478
>500	0.0035912451659079227	0.5664976754200248
>1k	0.0057459922654526764	3.2965326179572263
>5k	7.182490331815846E-4	0.9295497826826143
>10k+	7.182490331815846E-4	2.000358658978133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12320	1.9771472589549366	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGT	5725	0.918763641032225	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	4922	0.7898960071896264	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	4302	0.6903967133136475	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2966	0.4759917832841186	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2321	0.37248042110668894	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	2161	0.346803183977404	TruSeq Adapter, Index 13 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	1376	0.22082423931185005	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1150	0.18455514186673513	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	1105	0.17733341892412377	No Hit
AACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	926	0.1486070098857363	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT	837	0.1343240467325716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41147772499679036	0.0
2	0.0	0.0	0.0	0.5230132237771216	0.0
3	0.0	0.0	0.0	0.8118821414815766	0.0
4	1.6048273205803055E-4	0.0	0.0	2.108261651046347	0.0
5	1.6048273205803055E-4	0.0	0.0	2.2664976248555657	0.0
6	1.6048273205803055E-4	0.0	0.0	3.046443702657594	0.0
7	1.6048273205803055E-4	0.0	0.0	3.6649441520092436	0.0
8	1.6048273205803055E-4	0.0	0.0	4.061978431120812	0.0
9	1.6048273205803055E-4	0.0	0.0	5.606785209911414	0.0
10	1.6048273205803055E-4	0.0	0.0	6.515117473359867	0.0
11	1.6048273205803055E-4	0.0	0.0	8.110957760944922	0.0
12	1.6048273205803055E-4	0.0	0.0	8.528052381563743	0.0
13	1.6048273205803055E-4	0.0	0.0	8.750320965464116	0.0
14	1.6048273205803055E-4	0.0	0.0	9.074977532417511	0.0
15	1.6048273205803055E-4	0.0	0.0	9.255039157786623	0.0
16	1.6048273205803055E-4	0.0	0.0	9.563005520605984	0.0
17	1.6048273205803055E-4	0.0	0.0	9.93163435614328	0.0
18	1.6048273205803055E-4	0.0	0.0	10.599563486968803	0.0
19	1.6048273205803055E-4	0.0	0.0	10.805623314931314	0.0
20	1.6048273205803055E-4	0.0	0.0	11.036878931826935	0.0
21	1.6048273205803055E-4	0.0	0.0	11.25738220567467	0.0
22	1.6048273205803055E-4	0.0	0.0	11.49120554628322	0.0
23	1.6048273205803055E-4	0.0	0.0	11.736262678135832	0.0
24	1.6048273205803055E-4	0.0	0.0	11.923706509179612	0.0
25	1.6048273205803055E-4	0.0	0.0	12.080979586596483	0.0
26	1.6048273205803055E-4	0.0	0.0	12.222846321735782	0.0
27	1.6048273205803055E-4	0.0	0.0	12.396167672358454	0.0
28	1.6048273205803055E-4	0.0	0.0	12.562588265502631	0.0
29	1.6048273205803055E-4	0.0	0.0	12.728687893182693	0.0
30	1.6048273205803055E-4	0.0	0.0	12.977757093336757	0.0
31	1.6048273205803055E-4	0.0	0.0	13.135511618949801	0.0
32	1.6048273205803055E-4	0.0	0.0	13.322794967261522	0.0
33	3.209654641160611E-4	0.0	0.0	13.491783284118629	0.0
34	3.209654641160611E-4	0.0	0.0	13.665586082937477	0.0
35	3.209654641160611E-4	0.0	0.0	13.869238669919117	0.0
36	3.209654641160611E-4	0.0	0.0	14.036943124919759	0.0
37	3.209654641160611E-4	0.0	0.0	14.193574271408396	0.0
38	3.209654641160611E-4	0.0	0.0	14.377006034150725	0.0
39	3.209654641160611E-4	0.0	0.0	14.55995634869688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	55	1.8189894E-12	45.000004	2
TTAGGTA	30	2.1638953E-6	45.000004	37
CGTGACG	30	2.1638953E-6	45.000004	1
ATTCGCA	30	2.1638953E-6	45.000004	15
GATATCA	30	2.1638953E-6	45.000004	9
CGCACTG	55	1.8189894E-12	45.000004	1
ACGTACT	30	2.1638953E-6	45.000004	27
AGGATAC	30	2.1638953E-6	45.000004	45
ATTGCCA	30	2.1638953E-6	45.000004	38
TATAAGC	30	2.1638953E-6	45.000004	21
TACGTAG	30	2.1638953E-6	45.000004	1
TCAAACG	30	2.1638953E-6	45.000004	1
ACAACGT	45	3.8380676E-10	45.0	36
CTAGATC	20	7.030798E-4	45.0	16
ATGATTG	20	7.030798E-4	45.0	28
ATTAGTG	20	7.030798E-4	45.0	12
ATTAGCC	20	7.030798E-4	45.0	42
CGTATAG	20	7.030798E-4	45.0	1
CTACTAG	85	0.0	45.0	1
CAAGTCG	20	7.030798E-4	45.0	30
>>END_MODULE