Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548574_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 645947 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15387 | 2.382083979026143 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3557 | 0.5506643733928636 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3196 | 0.4947774353004194 | No Hit |
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT | 2314 | 0.3582337250579382 | Illumina Single End Adapter 1 (95% over 21bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1607 | 0.2487820208159493 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGT | 1582 | 0.2449117342444504 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT | 1287 | 0.19924235270076338 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC | 1171 | 0.18128422300900848 | No Hit |
GAACTGGGGTGATGACTGTAGCAAGAAGCCCTTGTTTCTCGGTGTAAATGG | 883 | 0.13669852170534116 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCCGGAT | 25 | 3.8889008E-5 | 45.000004 | 14 |
GGTACGC | 20 | 7.0309883E-4 | 45.000004 | 37 |
CGAGTTC | 20 | 7.0309883E-4 | 45.000004 | 40 |
CGTATAG | 20 | 7.0309883E-4 | 45.000004 | 1 |
CGTATAA | 40 | 6.8066583E-9 | 45.000004 | 34 |
GATCGCA | 20 | 7.0309883E-4 | 45.000004 | 40 |
TTTACGA | 45 | 3.8380676E-10 | 45.000004 | 32 |
CACGGAT | 40 | 6.8066583E-9 | 45.000004 | 33 |
AGATACG | 20 | 7.0309883E-4 | 45.000004 | 1 |
GAATGCG | 25 | 3.8889008E-5 | 45.000004 | 1 |
CGAAACA | 20 | 7.0309883E-4 | 45.000004 | 18 |
ACGTTGT | 20 | 7.0309883E-4 | 45.000004 | 14 |
GCGAATC | 25 | 3.8889008E-5 | 45.000004 | 40 |
CGACGCG | 20 | 7.0309883E-4 | 45.000004 | 1 |
CCTAGCG | 40 | 6.8066583E-9 | 45.000004 | 1 |
TAGACCG | 20 | 7.0309883E-4 | 45.000004 | 26 |
TTTCGGC | 20 | 7.0309883E-4 | 45.000004 | 32 |
TAGCGTT | 20 | 7.0309883E-4 | 45.000004 | 15 |
TAGGTAC | 25 | 3.8889008E-5 | 45.000004 | 22 |
ATTACGG | 40 | 6.8066583E-9 | 45.000004 | 2 |