FastQCFastQC Report
Sat 18 Jun 2016
SRR3548574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645947
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153872.382083979026143No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35570.5506643733928636No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31960.4947774353004194No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT23140.3582337250579382Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16070.2487820208159493No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGT15820.2449117342444504No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT12870.19924235270076338No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC11710.18128422300900848No Hit
GAACTGGGGTGATGACTGTAGCAAGAAGCCCTTGTTTCTCGGTGTAAATGG8830.13669852170534116No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT253.8889008E-545.00000414
GGTACGC207.0309883E-445.00000437
CGAGTTC207.0309883E-445.00000440
CGTATAG207.0309883E-445.0000041
CGTATAA406.8066583E-945.00000434
GATCGCA207.0309883E-445.00000440
TTTACGA453.8380676E-1045.00000432
CACGGAT406.8066583E-945.00000433
AGATACG207.0309883E-445.0000041
GAATGCG253.8889008E-545.0000041
CGAAACA207.0309883E-445.00000418
ACGTTGT207.0309883E-445.00000414
GCGAATC253.8889008E-545.00000440
CGACGCG207.0309883E-445.0000041
CCTAGCG406.8066583E-945.0000041
TAGACCG207.0309883E-445.00000426
TTTCGGC207.0309883E-445.00000432
TAGCGTT207.0309883E-445.00000415
TAGGTAC253.8889008E-545.00000422
ATTACGG406.8066583E-945.0000042