##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645947
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636078501796586	31.0	31.0	34.0	30.0	34.0
2	31.83177102765397	33.0	31.0	34.0	30.0	34.0
3	32.00248936832279	33.0	31.0	34.0	30.0	34.0
4	35.73573218855417	37.0	35.0	37.0	33.0	37.0
5	35.48037842113982	37.0	35.0	37.0	33.0	37.0
6	34.1141347509935	37.0	35.0	37.0	32.0	37.0
7	34.95424392403711	37.0	35.0	37.0	32.0	37.0
8	35.661949045355115	37.0	35.0	37.0	33.0	37.0
9	37.34265969189423	39.0	37.0	39.0	34.0	39.0
10	36.688801093588175	38.0	37.0	39.0	32.0	39.0
11	36.71058925887108	39.0	37.0	39.0	32.0	39.0
12	36.78032253420172	39.0	37.0	39.0	32.0	39.0
13	36.80797340958314	39.0	37.0	39.0	33.0	39.0
14	37.96201545947268	40.0	37.0	41.0	33.0	41.0
15	37.95449317049231	40.0	37.0	41.0	33.0	41.0
16	37.841867831261695	40.0	37.0	41.0	33.0	41.0
17	37.76828439484973	39.0	37.0	41.0	33.0	41.0
18	37.63696866770803	39.0	37.0	41.0	33.0	41.0
19	37.59753044754446	39.0	36.0	41.0	32.0	41.0
20	37.494693837110475	39.0	36.0	41.0	32.0	41.0
21	37.44477797714054	39.0	36.0	41.0	32.0	41.0
22	37.41448292197347	39.0	36.0	41.0	32.0	41.0
23	37.252856658518425	39.0	35.0	41.0	32.0	41.0
24	37.10823333802928	39.0	35.0	40.0	31.0	41.0
25	37.14097751053879	39.0	35.0	40.0	32.0	41.0
26	37.091942527792526	39.0	35.0	40.0	32.0	41.0
27	37.06508893144484	39.0	35.0	40.0	31.0	41.0
28	36.96101847365186	39.0	35.0	40.0	31.0	41.0
29	36.7405948166026	39.0	35.0	40.0	31.0	41.0
30	36.79371682196837	39.0	35.0	40.0	31.0	41.0
31	36.68991263989151	39.0	35.0	40.0	31.0	41.0
32	36.604661063523785	39.0	35.0	40.0	30.0	41.0
33	36.574621447270445	39.0	35.0	40.0	30.0	41.0
34	36.51753162411158	39.0	35.0	40.0	30.0	41.0
35	36.385423262280035	39.0	35.0	40.0	30.0	41.0
36	36.308800876852125	39.0	35.0	40.0	30.0	41.0
37	36.26531588504939	39.0	35.0	40.0	30.0	41.0
38	36.17461030084512	38.0	35.0	40.0	30.0	41.0
39	36.08508437998783	38.0	35.0	40.0	30.0	41.0
40	35.97734179429582	38.0	35.0	40.0	29.0	41.0
41	35.85430538418787	38.0	35.0	40.0	29.0	41.0
42	35.730683786750305	38.0	35.0	40.0	28.0	41.0
43	35.6436410417573	38.0	35.0	40.0	28.0	41.0
44	35.5281532385784	38.0	35.0	40.0	27.0	41.0
45	35.36748370996382	38.0	34.0	40.0	27.0	41.0
46	35.23345258976356	38.0	34.0	40.0	26.0	41.0
47	35.15354355697913	38.0	34.0	40.0	26.0	41.0
48	35.03992587627158	38.0	34.0	40.0	26.0	41.0
49	34.84925853049863	38.0	34.0	40.0	25.0	41.0
50	34.74189213666137	37.0	34.0	40.0	24.0	41.0
51	33.62255107617188	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	5.0
11	2.0
12	7.0
13	9.0
14	19.0
15	33.0
16	95.0
17	170.0
18	374.0
19	771.0
20	1207.0
21	1933.0
22	2640.0
23	3490.0
24	4271.0
25	4745.0
26	5637.0
27	6706.0
28	8203.0
29	10130.0
30	13177.0
31	16851.0
32	21916.0
33	29263.0
34	41871.0
35	59394.0
36	64248.0
37	86631.0
38	127258.0
39	134878.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.795277321513993	32.980569613296446	20.473661151766322	26.750491913423236
2	22.72833529685872	32.85486270545416	20.683895118330142	23.732906879356975
3	19.14832021822224	34.1289610447916	20.775079069954653	25.94763966703151
4	18.279673100115023	35.49811362232505	19.006667729705377	27.215545547854546
5	18.205208786479385	37.15180966859511	16.634646495765132	28.008335049160383
6	17.720184473339142	42.15051699288022	18.75664721718655	21.372651316594084
7	82.96624955298189	9.626021949169203	4.104671126268873	3.3030573715800213
8	84.81965238634129	8.58847552508178	3.16542998109752	3.4264421074794065
9	78.37020684359553	12.653360105395645	5.734216584332771	3.2422164666760587
10	29.02683966331603	47.53578853992665	11.537014646712501	11.900357150044819
11	19.610587246322066	30.209134805177513	33.0093645453884	17.17091340311202
12	22.637306156697065	27.62811809637633	33.182288949402974	16.552286797523635
13	22.776946096196745	27.61588799081039	29.61744539412676	19.9897205188661
14	19.187642329788666	31.406601470399277	29.80538651003875	19.60036968977331
15	17.919272014577047	33.89256394100445	29.129479663192182	19.058684381226325
16	20.82694091001274	32.74788798461793	27.33057046475949	19.094600640609833
17	18.657103446567596	31.30040080687734	29.48229498705002	20.560200759505037
18	19.67049928244887	31.323777337769197	27.69035230444603	21.3153710753359
19	19.472650232913846	33.10519284089871	28.40403314823043	19.018123777957015
20	21.144149597412788	32.928707773238365	26.841830676510614	19.085311952838236
21	21.719583804863245	32.75965365579529	27.713264400949306	17.80749813839216
22	19.10187677936425	30.974058243168557	27.79825589405942	22.12580908340777
23	19.0000108368024	34.06332098453898	26.860253240590946	20.076414938067675
24	18.182606312901832	32.92065757716964	27.655984159691123	21.240751950237403
25	19.52791792515485	33.83296152780336	26.000585187329612	20.638535359712172
26	21.3923123723773	33.19188726010029	24.856528476794537	20.559271890727874
27	17.70470332705315	31.476885874537697	27.685243526171654	23.133167272237507
28	20.422108934633957	31.34823754890107	28.568597733250563	19.66105578321441
29	20.469790865194824	32.44910186129822	26.75312370829186	20.327983565215103
30	20.785451437966273	29.612336615852385	27.83572026807153	21.766491678109816
31	21.301128420752786	31.625969313271835	25.709384825690034	21.36351744028535
32	20.57924256943681	30.71382017410097	27.099746573635297	21.607190682826918
33	18.909446131029327	29.885424036337348	29.66295996420759	21.542169868425738
34	21.032375721227904	28.960425545749107	29.338784760978843	20.668413972044146
35	20.344548391741117	28.2869956823083	30.145352482479215	21.22310344347137
36	20.44362772797149	30.844326237291913	28.137447809185584	20.574598225551014
37	19.455311349073533	31.084284004724843	27.885724370575293	21.574680275626328
38	20.279527577339937	29.827369737764865	30.607309887653322	19.28579279724188
39	20.671510201301345	28.52602458096407	28.670928110200993	22.131537107533592
40	21.970997620547816	29.34095212145888	28.33699978481207	20.351050473181235
41	20.321171860849265	27.93046488334182	30.740757368638604	21.00760588717031
42	22.006139822617026	28.62432985988014	28.906705968136702	20.462824349366123
43	20.06232709494742	28.582221141982238	29.169885454998628	22.185566308071714
44	21.74079297527506	27.953067356919377	26.689031762667838	23.617107905137726
45	20.727397139393787	28.111594294887972	29.135207687317997	22.02580087840024
46	19.231608785240894	29.1220487129749	30.41766584564988	21.228676656134326
47	20.879421995922264	30.006022165905254	28.415644007944923	20.698911830227555
48	21.026647697102085	27.773021625613246	30.287159782458932	20.913170894825736
49	19.73644896562721	27.87426832232366	30.781627594833633	21.6076551172155
50	19.531323777337768	29.034735047921888	28.412392967224864	23.021548207515476
51	19.935846129790836	27.007943376159343	30.18111393039986	22.875096563649958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17665.0
1	13390.5
2	9116.0
3	5573.5
4	2031.0
5	1762.5
6	1494.0
7	1621.5
8	1749.0
9	1835.5
10	1922.0
11	2208.0
12	2494.0
13	2574.0
14	2654.0
15	2634.0
16	2614.0
17	2729.5
18	2845.0
19	3278.0
20	3711.0
21	3203.5
22	2696.0
23	4098.5
24	5501.0
25	5769.0
26	7338.5
27	8640.0
28	10407.5
29	12175.0
30	13160.0
31	14145.0
32	17857.5
33	21570.0
34	24182.0
35	26794.0
36	27732.0
37	28670.0
38	26799.5
39	24929.0
40	28834.5
41	32740.0
42	33648.5
43	34557.0
44	38760.0
45	42963.0
46	46258.0
47	49553.0
48	50972.5
49	52392.0
50	50808.5
51	49225.0
52	45039.0
53	40853.0
54	35639.5
55	30426.0
56	28828.0
57	27230.0
58	25374.0
59	23518.0
60	20868.0
61	18218.0
62	16552.5
63	14887.0
64	12148.5
65	9410.0
66	8171.5
67	6933.0
68	5504.0
69	4075.0
70	3382.5
71	2690.0
72	2659.5
73	2629.0
74	2092.5
75	1476.5
76	1397.0
77	976.5
78	556.0
79	372.5
80	189.0
81	224.0
82	259.0
83	159.0
84	59.0
85	54.0
86	49.0
87	50.5
88	52.0
89	41.5
90	31.0
91	24.0
92	17.0
93	17.0
94	17.0
95	15.5
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	645947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.488087970770575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23702977725414	18.913871797864257
2	9.293880035695633	4.551787038078061
3	3.5884718043193846	2.6362443967440874
4	1.8460832494696156	1.808281960575118
5	1.1339286118607708	1.3883871799910157
6	0.7936790702761112	1.1661409736088488
7	0.5696263352304209	0.9764341865313139
8	0.4159113178752132	0.8147898352133877
9	0.3274870158803522	0.7217577768746883
>10	3.2027801892355248	17.70435544808951
>50	0.9040179286861924	15.815233090666359
>100	0.6794489019412808	28.399647357899376
>500	0.0025519207584649047	0.4004153116276425
>1k	0.004465861327313583	2.298755714119833
>5k	0.0	0.0
>10k+	6.379801896162262E-4	2.403897932116479
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15387	2.382083979026143	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3557	0.5506643733928636	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3196	0.4947774353004194	No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	2314	0.3582337250579382	Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1607	0.2487820208159493	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGT	1582	0.2449117342444504	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT	1287	0.19924235270076338	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC	1171	0.18128422300900848	No Hit
GAACTGGGGTGATGACTGTAGCAAGAAGCCCTTGTTTCTCGGTGTAAATGG	883	0.13669852170534116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.54811462859956E-4	0.0	0.0	0.44492814425951355	0.0
2	1.54811462859956E-4	0.0	0.0	0.6093379178167868	0.0
3	1.54811462859956E-4	0.0	0.0	0.802078189077432	0.0
4	1.54811462859956E-4	0.0	0.0	1.7465829239860236	0.0
5	1.54811462859956E-4	0.0	0.0	1.9105282631547171	0.0
6	1.54811462859956E-4	0.0	0.0	2.750535260632838	0.0
7	1.54811462859956E-4	0.0	0.0	3.391454716873056	0.0
8	1.54811462859956E-4	0.0	0.0	3.9257090752027644	0.0
9	1.54811462859956E-4	0.0	0.0	4.860770310876899	0.0
10	1.54811462859956E-4	0.0	0.0	5.98439190831446	0.0
11	1.54811462859956E-4	0.0	0.0	7.059867140802574	0.0
12	1.54811462859956E-4	0.0	0.0	7.562385149245991	0.0
13	1.54811462859956E-4	0.0	0.0	7.793054228907325	0.0
14	1.54811462859956E-4	0.0	0.0	8.02186557101434	0.0
15	1.54811462859956E-4	0.0	0.0	8.19912469598899	0.0
16	1.54811462859956E-4	0.0	0.0	8.605659597459235	0.0
17	1.54811462859956E-4	0.0	0.0	9.11622780197137	0.0
18	1.54811462859956E-4	0.0	0.0	9.89307172260263	0.0
19	1.54811462859956E-4	0.0	0.0	10.159966684573192	0.0
20	1.54811462859956E-4	0.0	0.0	10.509066533322393	0.0
21	1.54811462859956E-4	0.0	0.0	10.817915401728005	0.0
22	1.54811462859956E-4	0.0	0.0	11.168253742180086	0.0
23	1.54811462859956E-4	0.0	0.0	11.515186230449247	0.0
24	1.54811462859956E-4	0.0	0.0	11.759478718842258	0.0
25	1.54811462859956E-4	0.0	0.0	12.000365355052349	0.0
26	1.54811462859956E-4	0.0	0.0	12.203013559936032	0.0
27	1.54811462859956E-4	0.0	0.0	12.452414826603421	0.0
28	1.54811462859956E-4	0.0	0.0	12.70166128180795	0.0
29	1.54811462859956E-4	0.0	0.0	12.9442508441095	0.0
30	1.54811462859956E-4	0.0	0.0	13.279882095589885	0.0
31	1.54811462859956E-4	0.0	0.0	13.51209928987982	0.0
32	1.54811462859956E-4	0.0	0.0	13.771563301633106	0.0
33	1.54811462859956E-4	0.0	0.0	14.036600526049352	0.0
34	1.54811462859956E-4	0.0	0.0	14.272997629836503	0.0
35	1.54811462859956E-4	0.0	0.0	14.543143632527126	0.0
36	1.54811462859956E-4	0.0	0.0	14.793938202360255	0.0
37	1.54811462859956E-4	0.0	0.0	15.048448247302023	0.0
38	1.54811462859956E-4	0.0	0.0	15.311008488312508	0.0
39	1.54811462859956E-4	0.0	0.0	15.594777899734808	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	25	3.8889008E-5	45.000004	14
GGTACGC	20	7.0309883E-4	45.000004	37
CGAGTTC	20	7.0309883E-4	45.000004	40
CGTATAG	20	7.0309883E-4	45.000004	1
CGTATAA	40	6.8066583E-9	45.000004	34
GATCGCA	20	7.0309883E-4	45.000004	40
TTTACGA	45	3.8380676E-10	45.000004	32
CACGGAT	40	6.8066583E-9	45.000004	33
AGATACG	20	7.0309883E-4	45.000004	1
GAATGCG	25	3.8889008E-5	45.000004	1
CGAAACA	20	7.0309883E-4	45.000004	18
ACGTTGT	20	7.0309883E-4	45.000004	14
GCGAATC	25	3.8889008E-5	45.000004	40
CGACGCG	20	7.0309883E-4	45.000004	1
CCTAGCG	40	6.8066583E-9	45.000004	1
TAGACCG	20	7.0309883E-4	45.000004	26
TTTCGGC	20	7.0309883E-4	45.000004	32
TAGCGTT	20	7.0309883E-4	45.000004	15
TAGGTAC	25	3.8889008E-5	45.000004	22
ATTACGG	40	6.8066583E-9	45.000004	2
>>END_MODULE