FastQCFastQC Report
Sat 18 Jun 2016
SRR3548573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639503
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154732.419535170280671No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35590.5565259271653143No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30310.47396181096883044No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT22650.3541812939110528Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15090.23596449117517823No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGT14150.22126557654928905No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT11920.18639474717084986No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC10340.16168806088478083No Hit
GAACTGGGGTGATGACTGTAGCAAGAAGCCCTTGTTTCTCGGTGTAAATGG8330.13025740301452846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG650.045.0000041
CCTAGCG650.045.0000041
ACGTCAT650.045.00000437
TCATAGA650.045.00000415
TCGGTGT1150.045.00000439
ACGCGAG351.2107921E-745.01
ACAACGA900.045.013
AACCGAT207.0309377E-445.032
TCACCGA253.8888575E-545.044
CTCCGTA253.8888575E-545.043
CGTATAA453.8380676E-1045.034
AGATACG253.8888575E-545.01
CACGGAT502.1827873E-1145.033
CGACGAG502.1827873E-1145.01
TTAGCGA207.0309377E-445.030
CTATCCG453.8380676E-1045.01
TAGACCG351.2107921E-745.026
ACGCAAC253.8888575E-545.026
CCGGCAT253.8888575E-545.019
GGCGTTG207.0309377E-445.01