##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639503
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62291341870171	31.0	31.0	34.0	30.0	34.0
2	31.825591123106538	33.0	31.0	34.0	30.0	34.0
3	31.991656020378326	33.0	31.0	34.0	30.0	34.0
4	35.74168534002186	37.0	35.0	37.0	35.0	37.0
5	35.50516103911944	37.0	35.0	37.0	33.0	37.0
6	32.69222349230574	37.0	35.0	37.0	26.0	37.0
7	34.24560009882674	37.0	35.0	37.0	28.0	37.0
8	35.37443921295131	37.0	35.0	37.0	32.0	37.0
9	37.22314516116422	39.0	37.0	39.0	34.0	39.0
10	36.65426589085587	38.0	35.0	39.0	32.0	39.0
11	36.72811386342207	39.0	37.0	39.0	32.0	39.0
12	36.80203845799003	39.0	37.0	39.0	32.0	39.0
13	36.851340806845315	39.0	37.0	39.0	33.0	39.0
14	37.97180153963312	40.0	37.0	41.0	33.0	41.0
15	38.009613715651064	40.0	37.0	41.0	33.0	41.0
16	37.92959063522767	40.0	37.0	41.0	33.0	41.0
17	37.88096850210241	39.0	37.0	41.0	33.0	41.0
18	37.73012010889707	39.0	37.0	41.0	33.0	41.0
19	37.70186848224324	39.0	36.0	41.0	33.0	41.0
20	37.57713724564232	39.0	36.0	41.0	32.0	41.0
21	37.51127047097512	39.0	36.0	41.0	32.0	41.0
22	37.445886884033385	39.0	36.0	41.0	32.0	41.0
23	37.294050223376594	39.0	36.0	41.0	32.0	41.0
24	37.21274333349492	39.0	35.0	41.0	32.0	41.0
25	37.20199279753184	39.0	35.0	41.0	32.0	41.0
26	37.15870449395859	39.0	35.0	40.0	32.0	41.0
27	37.09745536768397	39.0	35.0	40.0	32.0	41.0
28	37.02865506494887	39.0	35.0	40.0	31.0	41.0
29	36.78674064077885	39.0	35.0	40.0	31.0	41.0
30	36.84142998547309	39.0	35.0	40.0	31.0	41.0
31	36.421682150044646	39.0	35.0	40.0	30.0	41.0
32	36.522476047805874	39.0	35.0	40.0	30.0	41.0
33	36.551737833911645	39.0	35.0	40.0	30.0	41.0
34	36.53391618178492	39.0	35.0	40.0	30.0	41.0
35	36.49278736769022	39.0	35.0	40.0	30.0	41.0
36	36.40148365214862	39.0	35.0	40.0	30.0	41.0
37	36.3330883514229	39.0	35.0	40.0	30.0	41.0
38	36.242552419613354	39.0	35.0	40.0	30.0	41.0
39	36.13037155416003	39.0	35.0	40.0	30.0	41.0
40	36.041111613237156	38.0	35.0	40.0	30.0	41.0
41	35.98262713388365	38.0	35.0	40.0	29.0	41.0
42	35.851034318838224	38.0	35.0	40.0	29.0	41.0
43	35.80137387940322	38.0	35.0	40.0	29.0	41.0
44	35.630328552016174	38.0	35.0	40.0	28.0	41.0
45	35.45894233490695	38.0	34.0	40.0	27.0	41.0
46	35.22742191983462	38.0	34.0	40.0	26.0	41.0
47	35.23110133963406	38.0	34.0	40.0	26.0	41.0
48	35.205783241048124	38.0	34.0	40.0	26.0	41.0
49	35.067525875562744	38.0	34.0	40.0	26.0	41.0
50	34.93710740997306	38.0	34.0	40.0	26.0	41.0
51	33.88752359253983	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	13.0
12	8.0
13	9.0
14	16.0
15	44.0
16	87.0
17	186.0
18	355.0
19	662.0
20	1185.0
21	1975.0
22	2565.0
23	3363.0
24	4015.0
25	4735.0
26	5303.0
27	6380.0
28	7733.0
29	9758.0
30	12905.0
31	16578.0
32	21679.0
33	29257.0
34	42250.0
35	59770.0
36	65190.0
37	88679.0
38	124776.0
39	130010.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.734074742417157	32.9584067627517	20.60334353396309	26.70417496086805
2	22.82553795681959	32.75434204374334	20.751114537382936	23.669005462054127
3	19.057611926761876	34.13009790415369	20.831645824960944	25.980644344123483
4	18.286700766063646	35.50913756464004	19.04072381208532	27.163437857210994
5	18.264339651260432	37.07379011513628	16.634011099244255	28.027859134359023
6	16.920952677313476	44.61042403241267	17.86903267068333	20.599590619590526
7	83.00164346375232	9.608086279501425	4.108346637936022	3.281923618810232
8	84.73220610380248	8.650624000200155	3.151822587227894	3.465347308769466
9	78.56835698972483	12.552873090509348	5.680505017177402	3.1982649025884164
10	29.222380504860805	47.56912790088553	11.40495040680028	11.803541187453382
11	19.777076886269494	30.188130470068163	32.86020550333618	17.17458714032616
12	22.58081666544176	27.708861412690794	33.15652936733682	16.553792554530627
13	22.775029984222122	27.675554297634257	29.65693671491768	19.892479003225944
14	19.26011293144833	31.35231578272502	29.890712006042193	19.496859279784456
15	17.888109985410544	33.869739469556826	29.170621560805817	19.071528984226813
16	20.87292788305919	32.87099513215732	27.261951859490885	18.99412512529261
17	18.671999974980572	31.23847738009048	29.418783023691837	20.670739621237118
18	19.828210344595725	31.32760909643895	27.719338298647543	21.124842260317777
19	19.68434862698064	33.00828924962041	28.388295285557692	18.91906683784126
20	21.02007340075027	33.00375447808689	26.817544249206026	19.158627871956817
21	21.649312043884077	32.618768012034344	27.88352830244737	17.848391641634205
22	19.146743643110355	30.911348343948347	27.933567160748268	22.00834085219303
23	19.052920783796168	33.92134204217963	26.873994336226726	20.151742837797475
24	18.159883534557306	32.93291821930468	27.714959898546216	21.1922383475918
25	19.440096449899375	33.89147509863128	26.14608531938083	20.522343132088512
26	21.449000239248292	33.23565331202512	24.808953202721487	20.5063932460051
27	17.7032789525616	31.433941670328363	27.728720584578966	23.134058792531075
28	20.360811442635924	31.48319867146831	28.550765203603422	19.605224682292345
29	20.39880970065817	32.612356783314546	26.715590075417943	20.27324344060935
30	20.751270908815126	29.75920363157014	27.730909784629628	21.758615674985105
31	21.395833952303587	31.68523056185819	25.607542106917403	21.31139337892082
32	20.543922389730778	30.741685340021863	27.18955188638677	21.524840383860592
33	18.92860549520487	29.983440265331048	29.63848488591922	21.449469353544863
34	21.01991702931808	28.960927470238605	29.366554965340274	20.65260053510304
35	20.25479161161089	28.475081430423316	30.092743896432072	21.17738306153372
36	20.41053755807244	30.858963914164594	28.095724335929624	20.634774191833348
37	19.425397535273486	31.226749522676204	27.859447101890062	21.488405840160247
38	20.26949052623678	29.867099919781452	30.619090137184656	19.244319416797108
39	20.742044994315897	28.656472291764075	28.49838077381967	22.10310194010036
40	21.971906308492688	29.388916080143485	28.270391225686197	20.36878638567763
41	20.275432640660014	28.112143336309604	30.63472728040369	20.977696742626694
42	22.013813852319693	28.562493061017697	28.98829247087191	20.435400615790698
43	20.144549751916724	28.648028234425798	29.08993390179561	22.117488111861867
44	21.935002650495775	27.875709730837855	26.674777131616274	23.5145104870501
45	20.728909794011912	28.089782221506386	29.19470276136312	21.986605223118577
46	19.33720404751815	29.10963670225159	30.330741216225725	21.22241803400453
47	20.827580167723998	30.11526138266748	28.362650370678484	20.694508078930042
48	20.978947714084217	27.878837159481662	30.335432359191433	20.806782767242687
49	19.810384001326028	27.937163703688643	30.724797225345306	21.527655069640016
50	19.515936594511675	29.08274081591486	28.54388486058705	22.857437728986415
51	19.952994747483594	27.115275456096377	30.320420701701167	22.61130909471887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17654.0
1	13341.0
2	9028.0
3	5608.5
4	2189.0
5	1846.5
6	1504.0
7	1596.0
8	1688.0
9	1776.5
10	1865.0
11	2105.0
12	2345.0
13	2438.5
14	2532.0
15	2597.5
16	2663.0
17	2743.0
18	2823.0
19	3202.0
20	3581.0
21	3157.5
22	2734.0
23	4175.0
24	5616.0
25	5877.0
26	7441.0
27	8744.0
28	10390.0
29	12036.0
30	13003.0
31	13970.0
32	17755.0
33	21540.0
34	24200.5
35	26861.0
36	27496.0
37	28131.0
38	26348.0
39	24565.0
40	28715.5
41	32866.0
42	33664.0
43	34462.0
44	38393.0
45	42324.0
46	45702.0
47	49080.0
48	50209.0
49	51338.0
50	49927.0
51	48516.0
52	44266.0
53	40016.0
54	35386.0
55	30756.0
56	28801.0
57	26846.0
58	25037.0
59	23228.0
60	20630.5
61	18033.0
62	16288.0
63	14543.0
64	11867.5
65	9192.0
66	8053.5
67	6915.0
68	5407.5
69	3900.0
70	3261.0
71	2622.0
72	2564.5
73	2507.0
74	2043.0
75	1451.5
76	1324.0
77	934.0
78	544.0
79	370.5
80	197.0
81	233.5
82	270.0
83	163.5
84	57.0
85	56.5
86	56.0
87	53.5
88	51.0
89	36.5
90	22.0
91	19.0
92	16.0
93	20.0
94	24.0
95	17.5
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.73000659735111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6620950008888	18.958541151387376
2	9.405706561783036	4.6520637065128625
3	3.622195166868489	2.6873073112065313
4	1.8857172841360206	1.8653520350969122
5	1.1811260500649452	1.4604627505204675
6	0.8137421256662548	1.20743088817614
7	0.6570358113919997	1.1373949965294066
8	0.4649827304664098	0.9199220793670918
9	0.35517777399920963	0.7905193824809642
>10	3.417925237812658	18.87909068804727
>50	0.8749134137013399	15.279277361776044
>100	0.6517081641532642	27.10507901117302
>500	0.002558226355851871	0.39524780422419475
>1k	0.004476896122740774	2.2150642249539207
>5k	0.0	0.0
>10k+	6.395565889629677E-4	2.4472466085478053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15473	2.419535170280671	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3559	0.5565259271653143	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3031	0.47396181096883044	No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	2265	0.3541812939110528	Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1509	0.23596449117517823	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGT	1415	0.22126557654928905	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT	1192	0.18639474717084986	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC	1034	0.16168806088478083	No Hit
GAACTGGGGTGATGACTGTAGCAAGAAGCCCTTGTTTCTCGGTGTAAATGG	833	0.13025740301452846	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4198572954309831	0.0
2	0.0	0.0	0.0	0.5657518416645426	0.0
3	0.0	0.0	0.0	0.7501137602169184	0.0
4	0.0	0.0	0.0	1.6397108379475938	0.0
5	0.0	0.0	0.0	1.8037444703152292	0.0
6	0.0	0.0	0.0	2.616563174840462	0.0
7	0.0	0.0	0.0	3.2387651035257066	0.0
8	0.0	0.0	0.0	3.73946642939908	0.0
9	0.0	0.0	0.0	4.665498050830098	0.0
10	0.0	0.0	0.0	5.751966761688374	0.0
11	0.0	0.0	0.0	6.789647585703273	0.0
12	0.0	0.0	0.0	7.270333368256287	0.0
13	0.0	0.0	0.0	7.505985116567084	0.0
14	0.0	0.0	0.0	7.736163864751221	0.0
15	0.0	0.0	0.0	7.915521897473507	0.0
16	0.0	0.0	0.0	8.331469907099732	0.0
17	0.0	0.0	0.0	8.848277490488707	0.0
18	0.0	0.0	0.0	9.602613279374765	0.0
19	0.0	0.0	0.0	9.868913828394863	0.0
20	0.0	0.0	0.0	10.229819093890098	0.0
21	0.0	0.0	0.0	10.5428747011351	0.0
22	0.0	0.0	0.0	10.892990337809206	0.0
23	0.0	0.0	0.0	11.251237288957206	0.0
24	0.0	0.0	0.0	11.50002423757199	0.0
25	0.0	0.0	0.0	11.716129556858998	0.0
26	0.0	0.0	0.0	11.919099675842022	0.0
27	0.0	0.0	0.0	12.15975530998291	0.0
28	0.0	0.0	0.0	12.388683086709523	0.0
29	0.0	0.0	0.0	12.627149520799746	0.0
30	0.0	0.0	0.0	12.978047014634802	0.0
31	0.0	0.0	0.0	13.217451677318167	0.0
32	0.0	0.0	0.0	13.46514402590762	0.0
33	0.0	0.0	0.0	13.72753528912296	0.0
34	0.0	0.0	0.0	13.97006738045013	0.0
35	0.0	0.0	0.0	14.239651729546226	0.0
36	0.0	0.0	0.0	14.484216649491872	0.0
37	0.0	0.0	0.0	14.741604026877122	0.0
38	0.0	0.0	0.0	14.994925747025425	0.0
39	0.0	0.0	0.0	15.260600810316761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	65	0.0	45.000004	1
CCTAGCG	65	0.0	45.000004	1
ACGTCAT	65	0.0	45.000004	37
TCATAGA	65	0.0	45.000004	15
TCGGTGT	115	0.0	45.000004	39
ACGCGAG	35	1.2107921E-7	45.0	1
ACAACGA	90	0.0	45.0	13
AACCGAT	20	7.0309377E-4	45.0	32
TCACCGA	25	3.8888575E-5	45.0	44
CTCCGTA	25	3.8888575E-5	45.0	43
CGTATAA	45	3.8380676E-10	45.0	34
AGATACG	25	3.8888575E-5	45.0	1
CACGGAT	50	2.1827873E-11	45.0	33
CGACGAG	50	2.1827873E-11	45.0	1
TTAGCGA	20	7.0309377E-4	45.0	30
CTATCCG	45	3.8380676E-10	45.0	1
TAGACCG	35	1.2107921E-7	45.0	26
ACGCAAC	25	3.8888575E-5	45.0	26
CCGGCAT	25	3.8888575E-5	45.0	19
GGCGTTG	20	7.0309377E-4	45.0	1
>>END_MODULE