Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548572_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 748184 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 13493 | 1.803433380024165 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2913 | 0.38934272852667257 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2437 | 0.32572201490542435 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1442 | 0.19273333832319323 | No Hit |
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT | 1354 | 0.18097152572094566 | TruSeq Adapter, Index 1 (96% over 27bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 800 | 0.10692556911134159 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGGA | 35 | 1.2111741E-7 | 45.000004 | 37 |
AATCCCG | 35 | 1.2111741E-7 | 45.000004 | 1 |
CGTATAA | 30 | 2.164481E-6 | 45.000004 | 21 |
TTAGACG | 30 | 2.164481E-6 | 45.000004 | 45 |
ATTACGT | 35 | 1.2111741E-7 | 45.000004 | 36 |
TTGTCGT | 30 | 2.164481E-6 | 45.000004 | 39 |
TAAGTCG | 30 | 2.164481E-6 | 45.000004 | 1 |
CGATTCG | 35 | 1.2111741E-7 | 45.000004 | 40 |
TCAATCG | 35 | 1.2111741E-7 | 45.000004 | 40 |
CGCTAAC | 35 | 1.2111741E-7 | 45.000004 | 35 |
TCGTCGT | 35 | 1.2111741E-7 | 45.000004 | 39 |
CGAGCAT | 35 | 1.2111741E-7 | 45.000004 | 42 |
TTACGTG | 35 | 1.2111741E-7 | 45.000004 | 37 |
CGGCTAT | 35 | 1.2111741E-7 | 45.000004 | 42 |
ATAGTCG | 30 | 2.164481E-6 | 45.000004 | 1 |
ATACGTA | 30 | 2.164481E-6 | 45.000004 | 18 |
CGATATT | 30 | 2.164481E-6 | 45.000004 | 31 |
GTACGAG | 35 | 1.2111741E-7 | 45.000004 | 40 |
TACCGAA | 30 | 2.164481E-6 | 45.000004 | 24 |
CAGGTAC | 20 | 7.0317055E-4 | 45.0 | 21 |