FastQCFastQC Report
Sat 18 Jun 2016
SRR3548572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748184
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134931.803433380024165No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29130.38934272852667257No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24370.32572201490542435No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14420.19273333832319323No Hit
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT13540.18097152572094566TruSeq Adapter, Index 1 (96% over 27bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8000.10692556911134159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA351.2111741E-745.00000437
AATCCCG351.2111741E-745.0000041
CGTATAA302.164481E-645.00000421
TTAGACG302.164481E-645.00000445
ATTACGT351.2111741E-745.00000436
TTGTCGT302.164481E-645.00000439
TAAGTCG302.164481E-645.0000041
CGATTCG351.2111741E-745.00000440
TCAATCG351.2111741E-745.00000440
CGCTAAC351.2111741E-745.00000435
TCGTCGT351.2111741E-745.00000439
CGAGCAT351.2111741E-745.00000442
TTACGTG351.2111741E-745.00000437
CGGCTAT351.2111741E-745.00000442
ATAGTCG302.164481E-645.0000041
ATACGTA302.164481E-645.00000418
CGATATT302.164481E-645.00000431
GTACGAG351.2111741E-745.00000440
TACCGAA302.164481E-645.00000424
CAGGTAC207.0317055E-445.021